##################################
# NO CUT ENZYMES ( 61 enzymes) #
##################################
AccI AflII AgeI AscI AseI
AsnI AvrII BamHI BbeI BbrPI
BciVI BclI BfrI BglII BlnI
BsaBI BsiWI BspDI Bst1107I BstYI
BstZ17I ClaI CpoI EcoRI EcoRV
FbaI FseI HincII HindII HpaI
KasI MamI MfeI MflI MluI
MunI NarI NcoI NdeI NheI
NotI PacI PaeR7I PinAI PmaCI
PmeI PmlI PshAI PshBI RsrII
SalI ScaI SfoI SgrAI SmlI
SnaBI SpeI SspI SwaI XhoI
XhoII
###################################
# ONE CUT ENZYMES ( 40 enzymes) #
###################################
Acc65I 1083 GGTACC G^GTACC
AccIII 3741 TCCGGA T^CCGGA
AclI 1536 AACGTT AA^CGTT
AhdI 2880 GACCTGCCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AlwI 2138 GATCC GGATC(4/5)
Asp718I 1083 GGTACC G^GTACC
AspEI 2880 GACCTGCCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 1928 GACGCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
BsaWI 3741 TCCGGA [AT]^CCGG[AT]
BseAI 3741 TCCGGA T^CCGGA
BsmI 3179 GAATGC GAATGC(1/-1)
BspEI 3741 TCCGGA T^CCGGA
BstBI 1570 TTCGAA TT^CGAA
BstEII 106 GGTCACC G^GT[ACGT]ACC
BstPI 106 GGTCACC G^GT[ACGT]ACC
DraI 1686 TTTAAA TTT^AAA
DrdI 3303 GACAGGGCGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI 890 CGGCCG C^GGCCG
Eam1105I 2880 GACCTGCCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI 890 CGGCCG C^GGCCG
Eco52I 890 CGGCCG C^GGCCG
EcoO65I 106 GGTCACC G^GT[ACGT]ACC
EcoT22I 1524 ATGCAT ATGCA^T
HindIII 1691 AAGCTT A^AGCTT
KpnI 1083 GGTACC GGTAC^C
KspI 2549 CCGCGG CCGC^GG
MroI 3741 TCCGGA T^CCGGA
NruI 3769 TCGCGA TCG^CGA
NsiI 1524 ATGCAT ATGCA^T
NspV 1570 TTCGAA TT^CGAA
PflFI 1928 GACGCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
Psp1406I 1536 AACGTT AA^CGTT
PvuI 2744 CGATCG CGAT^CG
SacII 2549 CCGCGG CCGC^GG
SbfI 4837 CCTGCAGG CCTGCA^GG
SexAI 553 ACCTGGT A^CC[AT]GGT
SfuI 1570 TTCGAA TT^CGAA
Sse8387I 4837 CCTGCAGG CCTGCA^GG
Tth111I 1928 GACGCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
XbaI 4757 TCTAGA T^CTAGA
###################################
# TWO CUT ENZYMES ( 20 enzymes) #
###################################
AatII 3416 GACGTC GACGT^C
4188 GACGTC
AcsI 1652 GAATTT [GA]^AATT[CT]
4498 GAATTT
Aor51HI 34 AGCGCT AGC^GCT
2561 AGCGCT
ApoI 1652 GAATTT [GA]^AATT[CT]
4498 GAATTT
BbvCI 1268 CCTCAGC CCTCAGC(-5/-2)
37 GCTGAGG
BsiEI 890 CGGCCG CG[GA][CT]^CG
2744 CGATCG
Bsp1407I 826 TGTACA T^GTACA
2653 TGTACA
BsrDI 1521 GCAATG GCAATG(2/0)
3043 GCAATG
BsrGI 826 TGTACA T^GTACA
2653 TGTACA
BssHII 2111 GCGCGC G^CGCGC
2481 GCGCGC
DraIII 702 CACTATGTG CAC[ACGT][ACGT][ACGT]^GTG
4212 CACTACGTG
Eco47III 34 AGCGCT AGC^GCT
2561 AGCGCT
NaeI 3702 GCCGGC GCC^GGC
4599 GCCGGC
NgoMIV 3702 GCCGGC G^CCGGC
4599 GCCGGC
PflMI 1301 CCAGCAAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2501 CCAGCGGCTGG
SapI 620 GAAGAGC GCTCTTC(1/4)
2160 GAAGAGC
SfiI 2597 GGCCCGCGTGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
2606 GGCCGTGCTGGCC
SphI 2629 GCATGC GCATG^C
3655 GCATGC
SspBI 826 TGTACA T^GTACA
2653 TGTACA
Van91I 1301 CCAGCAAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2501 CCAGCGGCTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 137 enzymes) #
#########################################
AccII AciI AcyI AfaI AflIII
AluI Alw44I AlwNI AocI ApaI
ApaLI Asp700I AspHI AvaI AvaII
AviII BalI BanI BanII BbsI
BbvI BcgI BcnI BfaI BglI
BlpI BmyI BpmI Bpu1102I BpuAI
BsaAI BsaHI BsaI BsaJI BseRI
BsgI BsiHKAI BsiYI BslI BsmAI
BsmBI BsmFI BsoBI Bsp1286I BspHI
BspLU11I BspMI BsrBI BsrFI BsrI
BssKI BssSI BstAPI BstF5I BstNI
BstUI BstXI Bsu36I Cac8I CelII
CfoI Cfr10I Cfr13I DdeI DpnII
DraII DsaI EaeI EarI Eco81I
EcoNI EcoO109I EcoRII EcoT14I FauI
Fnu4HI FokI FspI HaeII HaeIII
HapII HgaI HhaI Hin1I HinP1I
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeII MaeIII MboI MboII
MluNI MnlI MscI MseI MslI
MspA1I MspI MvaI MvnI MwoI
NciI NdeII NlaIII NlaIV NspI
PleI PpuMI PstI PvuII RcaI
RsaI SacI Sau3AI Sau96I ScrFI
SfaNI SfcI SmaI StuI StyI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I XcmI XmaCI
XmaI XmnI