##################################
# NO CUT ENZYMES ( 71 enzymes) #
##################################
AatII AccIII AgeI Aor51HI ApaI
AscI AspI AvaI AviII BalI
BbeI BbvCI BcgI BcnI BglI
BsaBI BsaI BseAI BsiEI BsiWI
BsoBI BspEI BsrBI BsrFI BstEII
BstPI Cfr10I CpoI EagI EclXI
Eco47III Eco52I EcoO65I FseI FspI
HaeII KasI KspI MamI MluI
MluNI MroI MscI NaeI NarI
NciI NgoMIV NheI NotI NruI
PaeR7I PflFI PinAI PmeI PvuI
RsrII SacI SacII SalI SbfI
SfiI SfoI SgrAI SmaI SnaBI
Sse8387I SwaI Tth111I XhoI XmaCI
XmaI
###################################
# ONE CUT ENZYMES ( 54 enzymes) #
###################################
Acc65I 6802 GGTACC G^GTACC
AccII 7265 CGCG CG^CG
AclI 2977 AACGTT AA^CGTT
AcyI 3211 GGCGTC G[GA]^CG[CT]C
AflII 5781 CTTAAG C^TTAAG
AhdI 1233 GACAGTTTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Asp718I 6802 GGTACC G^GTACC
AspEI 1233 GACAGTTTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvrII 3872 CCTAGG C^CTAGG
BbrPI 5802 CACGTG CAC^GTG
BbsI 455 GTCTTC GAAGAC(2/6)
BfrI 5781 CTTAAG C^TTAAG
BlnI 3872 CCTAGG C^CTAGG
BlpI 6739 GCTAAGC GC^T[ACGT]AGC
Bpu1102I 6739 GCTAAGC GC^T[ACGT]AGC
BpuAI 455 GTCTTC GAAGAC(2/6)
BsaHI 3211 GGCGTC G[GA]^CG[CT]C
BsaWI 6964 TCCGGT [AT]^CCGG[AT]
BsgI 1509 GTGCAG GTGCAG(16/14)
BsmBI 481 CGTCTC CGTCTC(1/5)
BspDI 4959 ATCGAT AT^CGAT
BssHII 7264 GCGCGC G^CGCGC
BssSI 2557 CTCGTG CACGAG(-5/-1)
Bst1107I 51 GTATAC GTA^TAC
BstBI 3331 TTCGAA TT^CGAA
BstUI 7265 CGCG CG^CG
BstXI 578 CCACCATGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstZ17I 51 GTATAC GTA^TAC
CelII 6739 GCTAAGC GC^T[ACGT]AGC
ClaI 4959 ATCGAT AT^CGAT
DraIII 420 CACCAAGTG CAC[ACGT][ACGT][ACGT]^GTG
EaeI 3446 TGGCCG [CT]^GGCC[GA]
Eam1105I 1233 GACAGTTTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoNI 7481 CCTAAAGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
FauI 5604 GCGGG CCCGC(4/6)
HapII 6965 CCGG C^CGG
Hin1I 3211 GGCGTC G[GA]^CG[CT]C
HpaI 3725 GTTAAC GTT^AAC
HpaII 6965 CCGG C^CGG
KpnI 6802 GGTACC GGTAC^C
MspI 6965 CCGG C^CGG
MvnI 7265 CGCG CG^CG
NspV 3331 TTCGAA TT^CGAA
PacI 6589 TTAATTAA TTAAT^TAA
PflMI 4170 CCACCCTTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI 5802 CACGTG CAC^GTG
PmlI 5802 CACGTG CAC^GTG
PshAI 3870 GACCTAGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
Psp1406I 2977 AACGTT AA^CGTT
SexAI 6805 ACCAGGT A^CC[AT]GGT
SfuI 3331 TTCGAA TT^CGAA
SphI 333 GCATGC GCATG^C
StuI 5785 AGGCCT AGG^CCT
Van91I 4170 CCACCCTTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
###################################
# TWO CUT ENZYMES ( 19 enzymes) #
###################################
Alw44I 2464 GTGCAC G^TGCAC
3080 GTGCAC
AocI 486 CCTGAGG CC^T[ACGT]AGG
5882 CCTCAGG
ApaLI 2464 GTGCAC G^TGCAC
3080 GTGCAC
AseI 188 ATTAAT AT^TAAT
5529 ATTAAT
AsnI 188 ATTAAT AT^TAAT
5529 ATTAAT
BamHI 384 GGATCC G^GATCC
4203 GGATCC
BanII 4975 GGGCTC G[GA]GC[CT]^C
7629 GAGCCC
BclI 4889 TGATCA T^GATCA
6227 TGATCA
BstAPI 1551 GCATCTGATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
3181 GCAATGACTGC
Bsu36I 486 CCTGAGG CC^T[ACGT]AGG
5882 CCTCAGG
DrdI 1818 GACCTGGCTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
5950 GACGCTCATGTC
Eco81I 486 CCTGAGG CC^T[ACGT]AGG
5882 CCTCAGG
EcoRV 2934 GATATC GAT^ATC
5523 GATATC
FbaI 4889 TGATCA T^GATCA
6227 TGATCA
HindIII 3653 AAGCTT A^AGCTT
3843 AAGCTT
PpuMI 3410 GGGTCCT [GA]G^G[AT]CC[CT]
3875 AGGTCCT
PshBI 188 ATTAAT AT^TAAT
5529 ATTAAT
SpeI 1773 ACTAGT A^CTAGT
7327 ACTAGT
XbaI 2099 TCTAGA T^CTAGA
4505 TCTAGA
#########################################
# 3 OR MORE CUT ENZYMES ( 114 enzymes) #
#########################################
AccI AciI AcsI AfaI AflIII
AluI AlwI AlwNI ApoI Asp700I
AspHI AvaII BanI BbvI BciVI
BfaI BglII BmyI BpmI BsaAI
BsaJI BseRI BsiHKAI BsiYI BslI
BsmAI BsmFI BsmI Bsp1286I Bsp1407I
BspHI BspLU11I BspMI BsrDI BsrGI
BsrI BssKI BstF5I BstNI BstYI
Cac8I CfoI Cfr13I DdeI DpnII
DraI DraII DsaI EarI EcoO109I
EcoRI EcoRII EcoT14I EcoT22I Fnu4HI
FokI HaeIII HgaI HhaI HinP1I
HincII HindII HinfI HphI ItaI
Ksp632I MaeI MaeII MaeIII MboI
MboII MfeI MflI MnlI MseI
MslI MspA1I MunI MvaI MwoI
NcoI NdeI NdeII NlaIII NlaIV
NsiI NspI PleI PstI PvuII
RcaI RsaI SapI Sau3AI Sau96I
ScaI ScrFI SfaNI SfcI SmlI
SspBI SspI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I XcmI XhoII XmnI