##################################
# NO CUT ENZYMES ( 66 enzymes) #
##################################
AatII Acc65I AccIII AclI AgeI
AscI Asp718I AspI BamHI BanI
BbeI BbvCI BcgI BciVI BsaBI
BseAI BsiWI Bsp1407I BspDI BspEI
BspHI BsrGI Bst1107I BstZ17I ClaI
CpoI DrdI EagI EclXI Eco52I
EcoRV KasI KpnI MamI MfeI
MluI MroI MunI NarI NheI
NotI NruI PacI PflFI PinAI
PmeI PshAI Psp1406I RcaI RsrII
SalI SbfI ScaI SexAI SfoI
SgrAI SmaI SnaBI SpeI Sse8387I
SspBI SwaI Tth111I XbaI XmaCI
XmaI
###################################
# ONE CUT ENZYMES ( 36 enzymes) #
###################################
AhdI 1738 GACTGATTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 404 GTGCAC G^TGCAC
AlwNI 2061 CAGTTTCTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 1410 CCTGAGG CC^T[ACGT]AGG
ApaLI 404 GTGCAC G^TGCAC
AspEI 1738 GACTGATTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AviII 1276 TGCGCA TGC^GCA
BalI 3869 TGGCCA TGG^CCA
BsaWI 2146 ACCGGA [AT]^CCGG[AT]
BsiEI 2807 CGATCG CG[GA][CT]^CG
BsmBI 15 CGTCTC CGTCTC(1/5)
BspLU11I 4271 ACATGT A^CATGT
BsrDI 2445 GCAATG GCAATG(2/0)
BstEII 669 GGTCACC G^GT[ACGT]ACC
BstPI 669 GGTCACC G^GT[ACGT]ACC
Bsu36I 1410 CCTGAGG CC^T[ACGT]AGG
Eam1105I 1738 GACTGATTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I 1410 CCTGAGG CC^T[ACGT]AGG
EcoO65I 669 GGTCACC G^GT[ACGT]ACC
FseI 22 GGCCGGCC GGCCGG^CC
FspI 1276 TGCGCA TGC^GCA
HpaI 2923 GTTAAC GTT^AAC
KspI 1813 CCGCGG CCGC^GG
MluNI 3869 TGGCCA TGG^CCA
MscI 3869 TGGCCA TGG^CCA
NdeI 1125 CATATG CA^TATG
PaeR7I 4518 CTCGAG C^TCGAG
PflMI 1630 CCACGATGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PvuI 2807 CGATCG CGAT^CG
SacII 1813 CCGCGG CCGC^GG
SapI 2857 GCTCTTC GCTCTTC(1/4)
SfiI 2158 GGCCCATGTGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SphI 1867 GCATGC GCATG^C
Van91I 1630 CCACGATGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 3595 CCAGGTGGGACCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 4518 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 31 enzymes) #
###################################
AccI 1896 GTCTAC GT^[AC][GT]AC
3709 GTCTAC
AflIII 4078 ACGTGT A^C[GA][CT]GT
4271 ACATGT
Aor51HI 1249 AGCGCT AGC^GCT
1459 AGCGCT
AvrII 2072 CCTAGG C^CTAGG
2579 CCTAGG
BclI 473 TGATCA T^GATCA
2770 TGATCA
BglII 144 AGATCT A^GATCT
3527 AGATCT
BlnI 2072 CCTAGG C^CTAGG
2579 CCTAGG
BlpI 1973 GCTCAGC GC^T[ACGT]AGC
2440 GCTCAGC
Bpu1102I 1973 GCTCAGC GC^T[ACGT]AGC
2440 GCTCAGC
BsmI 3035 GAATGC GAATGC(1/-1)
483 GCATTC
BspMI 638 GCAGGT ACCTGC(4/8)
2225 GCAGGT
BsrBI 1256 CCGCTC CCGCTC(-3/-3)
4135 CCGCTC
BssHII 1886 GCGCGC G^CGCGC
4052 GCGCGC
BssSI 524 CTCGTG CACGAG(-5/-1)
3379 CTCGTG
BstBI 1802 TTCGAA TT^CGAA
3835 TTCGAA
CelII 1973 GCTCAGC GC^T[ACGT]AGC
2440 GCTCAGC
DraI 84 TTTAAA TTT^AAA
3042 TTTAAA
Eco47III 1249 AGCGCT AGC^GCT
1459 AGCGCT
EcoNI 1274 CCTGCGCAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
2579 CCTAGGGAAGG
EcoRI 246 GAATTC G^AATTC
2504 GAATTC
EcoT22I 2023 ATGCAT ATGCA^T
4258 ATGCAT
FbaI 473 TGATCA T^GATCA
2770 TGATCA
HincII 642 GTCAAC GT[CT]^[GA]AC
2923 GTTAAC
HindII 642 GTCAAC GT[CT]^[GA]AC
2923 GTTAAC
HindIII 3613 AAGCTT A^AGCTT
3641 AAGCTT
NcoI 269 CCATGG C^CATGG
540 CCATGG
NsiI 2023 ATGCAT ATGCA^T
4258 ATGCAT
NspV 1802 TTCGAA TT^CGAA
3835 TTCGAA
SacI 2438 GAGCTC GAGCT^C
2462 GAGCTC
SfuI 1802 TTCGAA TT^CGAA
3835 TTCGAA
StuI 1282 AGGCCT AGG^CCT
2664 AGGCCT
#########################################
# 3 OR MORE CUT ENZYMES ( 125 enzymes) #
#########################################
AccII AciI AcsI AcyI AfaI
AflII AluI AlwI ApaI ApoI
AseI AsnI Asp700I AspHI AvaI
AvaII BanII BbrPI BbsI BbvI
BcnI BfaI BfrI BglI BmyI
BpmI BpuAI BsaAI BsaHI BsaI
BsaJI BseRI BsgI BsiHKAI BsiYI
BslI BsmAI BsmFI BsoBI Bsp1286I
BsrFI BsrI BssKI BstAPI BstF5I
BstNI BstUI BstXI BstYI Cac8I
CfoI Cfr10I Cfr13I DdeI DpnII
DraII DraIII DsaI EaeI EarI
EcoO109I EcoRII EcoT14I FauI Fnu4HI
FokI HaeII HaeIII HapII HgaI
HhaI Hin1I HinP1I HinfI HpaII
HphI ItaI Ksp632I MaeI MaeII
MaeIII MboI MboII MflI MnlI
MseI MslI MspA1I MspI MvaI
MvnI MwoI NaeI NciI NdeII
NgoMIV NlaIII NlaIV NspI PleI
PmaCI PmlI PpuMI PshBI PstI
PvuII RsaI Sau3AI Sau96I ScrFI
SfaNI SfcI SmlI SspI StyI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I XhoII XmnI