################################## # NO CUT ENZYMES ( 68 enzymes) # ################################## Acc65I AclI AcsI AflIII Alw44I Aor51HI ApaLI ApoI AscI AseI AsnI Asp718I AviII BamHI BbeI BcgI BciVI BsaBI BsiWI BspDI BspHI BspLU11I BsrBI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstZ17I ClaI CpoI DraI DrdI Eco47III EcoO65I EcoRI EcoRV FspI HaeII KasI KpnI MamI MluI NarI NdeI NheI NotI NruI NspV PacI PmeI PshAI PshBI Psp1406I PvuI RcaI RsrII SalI SbfI ScaI SfoI SfuI SnaBI Sse8387I SwaI XbaI ################################### # ONE CUT ENZYMES ( 29 enzymes) # ################################### AatII 1579 GACGTC GACGT^C AflII 148 CTTAAG C^TTAAG AhdI 301 GACAGCAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Asp700I 2745 GAACTCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AspEI 301 GACAGCAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AspI 3184 GACAGAGTC GAC[ACGT]^[ACGT][ACGT]GTC BbrPI 1183 CACGTG CAC^GTG BfrI 148 CTTAAG C^TTAAG Bsp1407I 2682 TGTACA T^GTACA BsrGI 2682 TGTACA T^GTACA Eam1105I 301 GACAGCAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EcoT22I 4484 ATGCAT ATGCA^T HindIII 4498 AAGCTT A^AGCTT HpaI 4175 GTTAAC GTT^AAC MfeI 2841 CAATTG C^AATTG MunI 2841 CAATTG C^AATTG NsiI 4484 ATGCAT ATGCA^T PaeR7I 2283 CTCGAG C^TCGAG PflFI 3184 GACAGAGTC GAC[ACGT]^[ACGT][ACGT]GTC PmaCI 1183 CACGTG CAC^GTG PmlI 1183 CACGTG CAC^GTG SexAI 4234 ACCAGGT A^CC[AT]GGT SgrAI 1940 CACCGGTG C[GA]^CCGG[CT]G SpeI 4699 ACTAGT A^CTAGT SspBI 2682 TGTACA T^GTACA SspI 2362 AATATT AAT^ATT Tth111I 3184 GACAGAGTC GAC[ACGT]^[ACGT][ACGT]GTC XhoI 2283 CTCGAG C^TCGAG XmnI 2745 GAACTCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 43 enzymes) # ################################### AccI 1210 GTCTAC GT^[AC][GT]AC 1334 GTCTAC AccIII 904 TCCGGA T^CCGGA 1238 TCCGGA AcyI 24 GACGCC G[GA]^CG[CT]C 1579 GACGTC AgeI 527 ACCGGT A^CCGGT 1941 ACCGGT AlwI 3404 GATCC GGATC(4/5) 1241 GGATC AvrII 2754 CCTAGG C^CTAGG 4954 CCTAGG BbvCI 414 CCTCAGC CCTCAGC(-5/-2) 1548 CCTCAGC BclI 4431 TGATCA T^GATCA 4477 TGATCA BglII 881 AGATCT A^GATCT 4395 AGATCT BlnI 2754 CCTAGG C^CTAGG 4954 CCTAGG BsaAI 1168 CACGTA [CT]AC^GT[GA] 1183 CACGTG BsaHI 24 GACGCC G[GA]^CG[CT]C 1579 GACGTC BseAI 904 TCCGGA T^CCGGA 1238 TCCGGA BsiEI 772 CGGCCG CG[GA][CT]^CG 1638 CGGCCG BsmBI 22 GAGACG CGTCTC(1/5) 3350 GAGACG BsmI 3609 GAATGC GAATGC(1/-1) 4812 GCATTC BspEI 904 TCCGGA T^CCGGA 1238 TCCGGA BsrDI 246 CATTGC GCAATG(2/0) 3217 GCAATG BstYI 881 AGATCT [GA]^GATC[CT] 4395 AGATCT DraIII 1672 CACTCGGTG CAC[ACGT][ACGT][ACGT]^GTG 2735 CACTATGTG EagI 772 CGGCCG C^GGCCG 1638 CGGCCG EclXI 772 CGGCCG C^GGCCG 1638 CGGCCG Eco52I 772 CGGCCG C^GGCCG 1638 CGGCCG FbaI 4431 TGATCA T^GATCA 4477 TGATCA FseI 1525 GGCCGGCC GGCCGG^CC 1639 GGCCGGCC Hin1I 24 GACGCC G[GA]^CG[CT]C 1579 GACGTC KspI 328 CCGCGG CCGC^GG 1647 CCGCGG MflI 881 AGATCT [GA]^GATC[CT] 4395 AGATCT MroI 904 TCCGGA T^CCGGA 1238 TCCGGA NaeI 1526 GCCGGC GCC^GGC 1640 GCCGGC NgoMIV 1526 GCCGGC G^CCGGC 1640 GCCGGC NspI 3001 GCATGC [GA]CATG^[CT] 3456 GCATGC PinAI 527 ACCGGT A^CCGGT 1941 ACCGGT SacII 328 CCGCGG CCGC^GG 1647 CCGCGG SapI 1314 GAAGAGC GCTCTTC(1/4) 4143 GCTCTTC SfiI 1065 GGCCCACCTGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC 3768 GGCCCTGTGGGCC SmaI 1076 CCCGGG CCC^GGG 2874 CCCGGG SphI 3001 GCATGC GCATG^C 3456 GCATGC TaqI 2284 TCGA T^CGA 3384 TCGA TthHB8I 2284 TCGA T^CGA 3384 TCGA XhoII 881 AGATCT [GA]^GATC[CT] 4395 AGATCT XmaCI 1076 CCCGGG C^CCGGG 2874 CCCGGG XmaI 1076 CCCGGG C^CCGGG 2874 CCCGGG ######################################### # 3 OR MORE CUT ENZYMES ( 118 enzymes) # ######################################### AccII AciI AfaI AluI AlwNI AocI ApaI AspHI AvaI AvaII BalI BanI BanII BbsI BbvI BcnI BfaI BglI BlpI BmyI BpmI Bpu1102I BpuAI BsaI BsaJI BsaWI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I BspMI BsrFI BsrI BssKI BstF5I BstNI BstUI BstXI Bsu36I Cac8I CelII CfoI Cfr10I Cfr13I DdeI DpnII DraII DsaI EaeI EarI Eco81I EcoNI EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeIII HapII HgaI HhaI HinP1I HincII HindII HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MluNI MnlI MscI MseI MslI MspA1I MspI MvaI MvnI MwoI NciI NcoI NdeII NlaIII NlaIV PflMI PleI PpuMI PstI PvuII RsaI SacI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI StuI StyI TfiI Tru9I TseI Tsp45I Tsp509I TspRI Van91I XcmI