##################################
# NO CUT ENZYMES (   68 enzymes) #
##################################

Acc65I      AclI        AcsI        AflIII      Alw44I      
Aor51HI     ApaLI       ApoI        AscI        AseI        
AsnI        Asp718I     AviII       BamHI       BbeI        
BcgI        BciVI       BsaBI       BsiWI       BspDI       
BspHI       BspLU11I    BsrBI       BssHII      BssSI       
Bst1107I    BstAPI      BstBI       BstEII      BstPI       
BstZ17I     ClaI        CpoI        DraI        DrdI        
Eco47III    EcoO65I     EcoRI       EcoRV       FspI        
HaeII       KasI        KpnI        MamI        MluI        
NarI        NdeI        NheI        NotI        NruI        
NspV        PacI        PmeI        PshAI       PshBI       
Psp1406I    PvuI        RcaI        RsrII       SalI        
SbfI        ScaI        SfoI        SfuI        SnaBI       
Sse8387I    SwaI        XbaI        


###################################
# ONE CUT ENZYMES (   29 enzymes) #
###################################

AatII        1579  GACGTC               GACGT^C
AflII         148  CTTAAG               C^TTAAG
AhdI          301  GACAGCAAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Asp700I      2745  GAACTCCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI         301  GACAGCAAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI         3184  GACAGAGTC            GAC[ACGT]^[ACGT][ACGT]GTC
BbrPI        1183  CACGTG               CAC^GTG
BfrI          148  CTTAAG               C^TTAAG
Bsp1407I     2682  TGTACA               T^GTACA
BsrGI        2682  TGTACA               T^GTACA
Eam1105I      301  GACAGCAAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoT22I      4484  ATGCAT               ATGCA^T
HindIII      4498  AAGCTT               A^AGCTT
HpaI         4175  GTTAAC               GTT^AAC
MfeI         2841  CAATTG               C^AATTG
MunI         2841  CAATTG               C^AATTG
NsiI         4484  ATGCAT               ATGCA^T
PaeR7I       2283  CTCGAG               C^TCGAG
PflFI        3184  GACAGAGTC            GAC[ACGT]^[ACGT][ACGT]GTC
PmaCI        1183  CACGTG               CAC^GTG
PmlI         1183  CACGTG               CAC^GTG
SexAI        4234  ACCAGGT              A^CC[AT]GGT
SgrAI        1940  CACCGGTG             C[GA]^CCGG[CT]G
SpeI         4699  ACTAGT               A^CTAGT
SspBI        2682  TGTACA               T^GTACA
SspI         2362  AATATT               AAT^ATT
Tth111I      3184  GACAGAGTC            GAC[ACGT]^[ACGT][ACGT]GTC
XhoI         2283  CTCGAG               C^TCGAG
XmnI         2745  GAACTCCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   43 enzymes) #
###################################

AccI         1210  GTCTAC               GT^[AC][GT]AC
             1334  GTCTAC              
AccIII        904  TCCGGA               T^CCGGA
             1238  TCCGGA              
AcyI           24  GACGCC               G[GA]^CG[CT]C
             1579  GACGTC              
AgeI          527  ACCGGT               A^CCGGT
             1941  ACCGGT              
AlwI         3404  GATCC                GGATC(4/5)
             1241  GGATC               
AvrII        2754  CCTAGG               C^CTAGG
             4954  CCTAGG              
BbvCI         414  CCTCAGC              CCTCAGC(-5/-2)
             1548  CCTCAGC             
BclI         4431  TGATCA               T^GATCA
             4477  TGATCA              
BglII         881  AGATCT               A^GATCT
             4395  AGATCT              
BlnI         2754  CCTAGG               C^CTAGG
             4954  CCTAGG              
BsaAI        1168  CACGTA               [CT]AC^GT[GA]
             1183  CACGTG              
BsaHI          24  GACGCC               G[GA]^CG[CT]C
             1579  GACGTC              
BseAI         904  TCCGGA               T^CCGGA
             1238  TCCGGA              
BsiEI         772  CGGCCG               CG[GA][CT]^CG
             1638  CGGCCG              
BsmBI          22  GAGACG               CGTCTC(1/5)
             3350  GAGACG              
BsmI         3609  GAATGC               GAATGC(1/-1)
             4812  GCATTC              
BspEI         904  TCCGGA               T^CCGGA
             1238  TCCGGA              
BsrDI         246  CATTGC               GCAATG(2/0)
             3217  GCAATG              
BstYI         881  AGATCT               [GA]^GATC[CT]
             4395  AGATCT              
DraIII       1672  CACTCGGTG            CAC[ACGT][ACGT][ACGT]^GTG
             2735  CACTATGTG           
EagI          772  CGGCCG               C^GGCCG
             1638  CGGCCG              
EclXI         772  CGGCCG               C^GGCCG
             1638  CGGCCG              
Eco52I        772  CGGCCG               C^GGCCG
             1638  CGGCCG              
FbaI         4431  TGATCA               T^GATCA
             4477  TGATCA              
FseI         1525  GGCCGGCC             GGCCGG^CC
             1639  GGCCGGCC            
Hin1I          24  GACGCC               G[GA]^CG[CT]C
             1579  GACGTC              
KspI          328  CCGCGG               CCGC^GG
             1647  CCGCGG              
MflI          881  AGATCT               [GA]^GATC[CT]
             4395  AGATCT              
MroI          904  TCCGGA               T^CCGGA
             1238  TCCGGA              
NaeI         1526  GCCGGC               GCC^GGC
             1640  GCCGGC              
NgoMIV       1526  GCCGGC               G^CCGGC
             1640  GCCGGC              
NspI         3001  GCATGC               [GA]CATG^[CT]
             3456  GCATGC              
PinAI         527  ACCGGT               A^CCGGT
             1941  ACCGGT              
SacII         328  CCGCGG               CCGC^GG
             1647  CCGCGG              
SapI         1314  GAAGAGC              GCTCTTC(1/4)
             4143  GCTCTTC             
SfiI         1065  GGCCCACCTGGCC        GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
             3768  GGCCCTGTGGGCC       
SmaI         1076  CCCGGG               CCC^GGG
             2874  CCCGGG              
SphI         3001  GCATGC               GCATG^C
             3456  GCATGC              
TaqI         2284  TCGA                 T^CGA
             3384  TCGA                
TthHB8I      2284  TCGA                 T^CGA
             3384  TCGA                
XhoII         881  AGATCT               [GA]^GATC[CT]
             4395  AGATCT              
XmaCI        1076  CCCGGG               C^CCGGG
             2874  CCCGGG              
XmaI         1076  CCCGGG               C^CCGGG
             2874  CCCGGG              


#########################################
# 3 OR MORE CUT ENZYMES (  118 enzymes) #
#########################################

AccII       AciI        AfaI        AluI        AlwNI       
AocI        ApaI        AspHI       AvaI        AvaII       
BalI        BanI        BanII       BbsI        BbvI        
BcnI        BfaI        BglI        BlpI        BmyI        
BpmI        Bpu1102I    BpuAI       BsaI        BsaJI       
BsaWI       BseRI       BsgI        BsiHKAI     BsiYI       
BslI        BsmAI       BsmFI       BsoBI       Bsp1286I    
BspMI       BsrFI       BsrI        BssKI       BstF5I      
BstNI       BstUI       BstXI       Bsu36I      Cac8I       
CelII       CfoI        Cfr10I      Cfr13I      DdeI        
DpnII       DraII       DsaI        EaeI        EarI        
Eco81I      EcoNI       EcoO109I    EcoRII      EcoT14I     
FauI        Fnu4HI      FokI        HaeIII      HapII       
HgaI        HhaI        HinP1I      HincII      HindII      
HinfI       HpaII       HphI        ItaI        Ksp632I     
MaeI        MaeII       MaeIII      MboI        MboII       
MluNI       MnlI        MscI        MseI        MslI        
MspA1I      MspI        MvaI        MvnI        MwoI        
NciI        NcoI        NdeII       NlaIII      NlaIV       
PflMI       PleI        PpuMI       PstI        PvuII       
RsaI        SacI        Sau3AI      Sau96I      ScrFI       
SfaNI       SfcI        SmlI        StuI        StyI        
TfiI        Tru9I       TseI        Tsp45I      Tsp509I     
TspRI       Van91I      XcmI