##################################
# NO CUT ENZYMES ( 95 enzymes) #
##################################
AatII Acc65I AccII AccIII AgeI
AhdI Alw44I AlwNI Aor51HI ApaI
ApaLI AscI Asp718I AspEI AvaII
AviII AvrII BalI BamHI BanII
BglI BlnI BlpI Bpu1102I BsaWI
BseAI BsiEI BsiWI BsmBI BsmI
Bsp1407I BspDI BspEI BspMI BsrBI
BsrFI BsrGI BssHII BstAPI BstUI
CelII Cfr10I Cfr13I ClaI CpoI
DraII DraIII DsaI EaeI EagI
Eam1105I EclXI Eco47III Eco52I EcoO109I
EcoRV FseI FspI KpnI KspI
MluI MluNI MroI MscI MvnI
NaeI NcoI NgoMIV NheI NotI
NruI PacI PaeR7I PflMI PinAI
PmeI PpuMI PshAI PstI PvuI
RsrII SacI SacII Sau96I SbfI
SexAI SfiI SgrAI SphI Sse8387I
SspBI StuI Van91I XcmI XhoI
###################################
# ONE CUT ENZYMES ( 44 enzymes) #
###################################
AclI 4511 AACGTT AA^CGTT
AcyI 2337 GGCGCC G[GA]^CG[CT]C
AlwI 1027 GGATC GGATC(4/5)
AspI 3297 GACATGGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 4392 CCCGGG C^[CT]CG[GA]G
BbeI 2337 GGCGCC GGCGC^C
BbrPI 1386 CACGTG CAC^GTG
BcgI 1358 CGAAGATCTTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI 4819 TGATCA T^GATCA
BsaHI 2337 GGCGCC G[GA]^CG[CT]C
BsoBI 4392 CCCGGG C^[CT]CG[GA]G
BssSI 3104 CACGAG CACGAG(-5/-1)
BstBI 2938 TTCGAA TT^CGAA
BstEII 1263 GGTCACC G^GT[ACGT]ACC
BstPI 1263 GGTCACC G^GT[ACGT]ACC
DrdI 2624 GACTGGGAGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoNI 1597 CCTAAGAAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 1263 GGTCACC G^GT[ACGT]ACC
EcoT22I 48 ATGCAT ATGCA^T
FauI 4337 GCGGG CCCGC(4/6)
FbaI 4819 TGATCA T^GATCA
HaeII 2337 GGCGCC [GA]GCGC^[CT]
HgaI 3292 GCGTC GACGC(5/10)
Hin1I 2337 GGCGCC G[GA]^CG[CT]C
HindIII 1707 AAGCTT A^AGCTT
KasI 2337 GGCGCC G^GCGCC
MfeI 2930 CAATTG C^AATTG
MunI 2930 CAATTG C^AATTG
NarI 2337 GGCGCC GG^CGCC
NsiI 48 ATGCAT ATGCA^T
NspV 2938 TTCGAA TT^CGAA
PflFI 3297 GACATGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PmaCI 1386 CACGTG CAC^GTG
PmlI 1386 CACGTG CAC^GTG
Psp1406I 4511 AACGTT AA^CGTT
SalI 3294 GTCGAC G^TCGAC
SfoI 2337 GGCGCC GGC^GCC
SfuI 2938 TTCGAA TT^CGAA
SmaI 4392 CCCGGG CCC^GGG
SnaBI 27 TACGTA TAC^GTA
SpeI 3167 ACTAGT A^CTAGT
Tth111I 3297 GACATGGTC GAC[ACGT]^[ACGT][ACGT]GTC
XmaCI 4392 CCCGGG C^CCGGG
XmaI 4392 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 36 enzymes) #
###################################
AocI 58 CCTAAGG CC^T[ACGT]AGG
4251 CCTAAGG
Asp700I 534 GAAAAAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
3892 GAATATATTC
AspHI 2750 GAGCAC G[AT]GC[AT]^C
4923 GTGCTC
BanI 2337 GGCGCC G^G[CT][GA]CC
4340 GGCACC
BbvCI 2648 GCTGAGG CCTCAGC(-5/-2)
4368 GCTGAGG
BciVI 3112 GGATAC GTATCC(6/5)
5030 GTATCC
BsaAI 27 TACGTA [CT]AC^GT[GA]
1386 CACGTG
BsaBI 1991 GATGACTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
2254 GATAATGATC
BsaI 4268 GAGACC GGTCTC(1/5)
3336 GGTCTC
BsgI 608 CTGCAC GTGCAG(16/14)
4194 GTGCAG
BsiHKAI 2750 GAGCAC G[AT]GC[AT]^C
4923 GTGCTC
BspHI 3469 TCATGA T^CATGA
3633 TCATGA
BspLU11I 450 ACATGT A^CATGT
1790 ACATGT
BsrDI 4429 CATTGC GCAATG(2/0)
4281 GCAATG
Bsu36I 58 CCTAAGG CC^T[ACGT]AGG
4251 CCTAAGG
CfoI 1383 GCGC GCG^C
2338 GCGC
EarI 2503 GAAGAG CTCTTC(1/4)
2822 GAAGAG
Eco81I 58 CCTAAGG CC^T[ACGT]AGG
4251 CCTAAGG
EcoRI 2935 GAATTC G^AATTC
5050 GAATTC
EcoT14I 840 CCAAGG C^C[AT][AT]GG
2562 CCAAGG
HaeIII 4188 GGCC GG^CC
4232 GGCC
HhaI 1383 GCGC GCG^C
2338 GCGC
HinP1I 1383 GCGC G^CGC
2338 GCGC
HpaI 3142 GTTAAC GTT^AAC
4018 GTTAAC
Ksp632I 2503 GAAGAG CTCTTC(1/4)
2822 GAAGAG
MamI 1991 GATGACTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
2254 GATAATGATC
NdeI 4030 CATATG CA^TATG
5101 CATATG
NlaIV 2337 GGCGCC GG[ACGT]^[ACGT]CC
4340 GGCACC
NspI 450 ACATGT [GA]CATG^[CT]
1790 ACATGT
PvuII 1055 CAGCTG CAG^CTG
2288 CAGCTG
RcaI 3469 TCATGA T^CATGA
3633 TCATGA
SapI 2503 GAAGAGC GCTCTTC(1/4)
2822 GAAGAGC
ScaI 3451 AGTACT AGT^ACT
3750 AGTACT
StyI 840 CCAAGG C^C[AT][AT]GG
2562 CCAAGG
SwaI 228 ATTTAAAT ATTT^AAAT
3508 ATTTAAAT
XmnI 534 GAAAAAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
3892 GAATATATTC
#########################################
# 3 OR MORE CUT ENZYMES ( 83 enzymes) #
#########################################
AccI AciI AcsI AfaI AflII
AflIII AluI ApoI AseI AsnI
BbsI BbvI BcnI BfaI BfrI
BglII BmyI BpmI BpuAI BsaJI
BseRI BsiYI BslI BsmAI BsmFI
Bsp1286I BsrI BssKI Bst1107I BstF5I
BstNI BstXI BstYI BstZ17I Cac8I
DdeI DpnII DraI EcoRII Fnu4HI
FokI HapII HincII HindII HinfI
HpaII HphI ItaI MaeI MaeII
MaeIII MboI MboII MflI MnlI
MseI MslI MspA1I MspI MvaI
MwoI NciI NdeII NlaIII PleI
PshBI RsaI Sau3AI ScrFI SfaNI
SfcI SmlI SspI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I XbaI XhoII