##################################
# NO CUT ENZYMES ( 67 enzymes) #
##################################
AatII AclI AcsI AgeI Alw44I
ApaLI ApoI AscI AseI AsnI
AspI AvrII BamHI BcgI BciVI
BclI BlnI BsaBI BsiWI BspDI
BssHII Bst1107I BstBI BstZ17I ClaI
CpoI DraI DrdI EagI EclXI
Eco52I EcoRI EcoRV EcoT22I FbaI
HpaI KspI MamI MfeI MunI
NdeI NotI NruI NsiI NspV
PacI PaeR7I PflFI PinAI PmeI
PshBI Psp1406I PvuI RsrII SacII
SalI SbfI ScaI SfuI SnaBI
SpeI SphI Sse8387I SwaI Tth111I
XbaI XhoI
###################################
# ONE CUT ENZYMES ( 41 enzymes) #
###################################
AccI 2868 GTAGAC GT^[AC][GT]AC
AcyI 825 GGCGCC G[GA]^CG[CT]C
AflII 1484 CTTAAG C^TTAAG
AhdI 3510 GACAACATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Asp700I 1387 GAACATTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI 3510 GACAACATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AviII 4051 TGCGCA TGC^GCA
BbeI 825 GGCGCC GGCGC^C
BfrI 1484 CTTAAG C^TTAAG
BglII 4722 AGATCT A^GATCT
BsaHI 825 GGCGCC G[GA]^CG[CT]C
BsmBI 3990 GAGACG CGTCTC(1/5)
Bsp1407I 5128 TGTACA T^GTACA
BspHI 4231 TCATGA T^CATGA
BspLU11I 3514 ACATGT A^CATGT
BsrGI 5128 TGTACA T^GTACA
BssSI 2068 CACGAG CACGAG(-5/-1)
BstEII 4783 GGTTACC G^GT[ACGT]ACC
BstPI 4783 GGTTACC G^GT[ACGT]ACC
Eam1105I 3510 GACAACATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoO65I 4783 GGTTACC G^GT[ACGT]ACC
FseI 2582 GGCCGGCC GGCCGG^CC
FspI 4051 TGCGCA TGC^GCA
Hin1I 825 GGCGCC G[GA]^CG[CT]C
HindIII 2965 AAGCTT A^AGCTT
KasI 825 GGCGCC G^GCGCC
MluI 4 ACGCGT A^CGCGT
NarI 825 GGCGCC GG^CGCC
NheI 2246 GCTAGC G^CTAGC
PshAI 53 GACCAGTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
RcaI 4231 TCATGA T^CATGA
SexAI 229 ACCTGGT A^CC[AT]GGT
SfiI 1446 GGCCGGCGGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfoI 825 GGCGCC GGC^GCC
SgrAI 1884 CACCGGCG C[GA]^CCGG[CT]G
SmaI 2716 CCCGGG CCC^GGG
SspBI 5128 TGTACA T^GTACA
SspI 4296 AATATT AAT^ATT
XmaCI 2716 CCCGGG C^CCGGG
XmaI 2716 CCCGGG C^CCGGG
XmnI 1387 GAACATTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 20 enzymes) #
###################################
AccIII 2599 TCCGGA T^CCGGA
3790 TCCGGA
AflIII 4 ACGCGT A^C[GA][CT]GT
3514 ACATGT
BalI 1183 TGGCCA TGG^CCA
2650 TGGCCA
BbrPI 2283 CACGTG CAC^GTG
3858 CACGTG
BsaWI 2599 TCCGGA [AT]^CCGG[AT]
3790 TCCGGA
BseAI 2599 TCCGGA T^CCGGA
3790 TCCGGA
BsiEI 29 CGACCG CG[GA][CT]^CG
3947 CGACCG
BsmI 2726 GAATGC GAATGC(1/-1)
1237 GCATTC
BspEI 2599 TCCGGA T^CCGGA
3790 TCCGGA
BsrDI 4803 CATTGC GCAATG(2/0)
406 GCAATG
HincII 1225 GTCAAC GT[CT]^[GA]AC
4733 GTCAAC
HindII 1225 GTCAAC GT[CT]^[GA]AC
4733 GTCAAC
MluNI 1183 TGGCCA TGG^CCA
2650 TGGCCA
MroI 2599 TCCGGA T^CCGGA
3790 TCCGGA
MscI 1183 TGGCCA TGG^CCA
2650 TGGCCA
NaeI 1447 GCCGGC GCC^GGC
2583 GCCGGC
NgoMIV 1447 GCCGGC G^CCGGC
2583 GCCGGC
NspI 466 GCATGT [GA]CATG^[CT]
3514 ACATGT
PmaCI 2283 CACGTG CAC^GTG
3858 CACGTG
PmlI 2283 CACGTG CAC^GTG
3858 CACGTG
#########################################
# 3 OR MORE CUT ENZYMES ( 130 enzymes) #
#########################################
Acc65I AccII AciI AfaI AluI
AlwI AlwNI AocI Aor51HI ApaI
Asp718I AspHI AvaI AvaII BanI
BanII BbsI BbvCI BbvI BcnI
BfaI BglI BlpI BmyI BpmI
Bpu1102I BpuAI BsaAI BsaI BsaJI
BseRI BsgI BsiHKAI BsiYI BslI
BsmAI BsmFI BsoBI Bsp1286I BspMI
BsrBI BsrFI BsrI BssKI BstAPI
BstF5I BstNI BstUI BstXI BstYI
Bsu36I Cac8I CelII CfoI Cfr10I
Cfr13I DdeI DpnII DraII DraIII
DsaI EaeI EarI Eco47III Eco81I
EcoNI EcoO109I EcoRII EcoT14I FauI
Fnu4HI FokI HaeII HaeIII HapII
HgaI HhaI HinP1I HinfI HpaII
HphI ItaI KpnI Ksp632I MaeI
MaeII MaeIII MboI MboII MflI
MnlI MseI MslI MspA1I MspI
MvaI MvnI MwoI NciI NcoI
NdeII NlaIII NlaIV PflMI PleI
PpuMI PstI PvuII RsaI SacI
SapI Sau3AI Sau96I ScrFI SfaNI
SfcI SmlI StuI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I Van91I XcmI XhoII