##################################
# NO CUT ENZYMES (   70 enzymes) #
##################################

AatII       Acc65I      AclI        AgeI        Aor51HI     
ApaI        AscI        Asp718I     AvaI        AviII       
AvrII       BamHI       BbrPI       BcgI        BglI        
BglII       BlnI        BsaAI       BsiEI       BsiWI       
BsmBI       BsoBI       BspDI       BsrBI       BssHII      
BstBI       ClaI        CpoI        EagI        EclXI       
Eco47III    Eco52I      EcoRV       FseI        FspI        
HpaI        KpnI        MfeI        MluI        MunI        
NaeI        NgoMIV      NheI        NotI        NruI        
NspV        PacI        PaeR7I      PinAI       PmaCI       
PmeI        PmlI        PshAI       Psp1406I    PvuI        
RsrII       SacI        SalI        ScaI        SfiI        
SfuI        SgrAI       SmaI        SnaBI       SphI        
StuI        SwaI        XhoI        XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   47 enzymes) #
###################################

AccI         3478  GTATAC               GT^[AC][GT]AC
AccIII       1032  TCCGGA               T^CCGGA
AcyI         2054  GGCGCC               G[GA]^CG[CT]C
Alw44I        172  GTGCAC               G^TGCAC
ApaLI         172  GTGCAC               G^TGCAC
Asp700I      3195  GAAGGTTTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspI          637  GACTGTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
BanI         2054  GGCGCC               G^G[CT][GA]CC
BanII        2470  GGGCTC               G[GA]GC[CT]^C
BbeI         2054  GGCGCC               GGCGC^C
BbvCI        2082  GCTGAGG              CCTCAGC(-5/-2)
BsaBI        4309  GATTTACATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI        2054  GGCGCC               G[GA]^CG[CT]C
BsaWI        1032  TCCGGA               [AT]^CCGG[AT]
BseAI        1032  TCCGGA               T^CCGGA
BsmI         2609  GAATGC               GAATGC(1/-1)
BspEI        1032  TCCGGA               T^CCGGA
BsrDI        2653  CATTGC               GCAATG(2/0)
BssSI        1155  CTCGTG               CACGAG(-5/-1)
Bst1107I     3478  GTATAC               GTA^TAC
BstAPI       1151  GCATCTCGTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII       2312  GGTTACC              G^GT[ACGT]ACC
BstPI        2312  GGTTACC              G^GT[ACGT]ACC
BstYI         790  GGATCT               [GA]^GATC[CT]
BstZ17I      3478  GTATAC               GTA^TAC
DraIII       1804  CACGGAGTG            CAC[ACGT][ACGT][ACGT]^GTG
EcoO65I      2312  GGTTACC              G^GT[ACGT]ACC
HaeII        2054  GGCGCC               [GA]GCGC^[CT]
HgaI         1018  GCGTC                GACGC(5/10)
Hin1I        2054  GGCGCC               G[GA]^CG[CT]C
KasI         2054  GGCGCC               G^GCGCC
KspI           48  CCGCGG               CCGC^GG
MamI         4309  GATTTACATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MflI          790  GGATCT               [GA]^GATC[CT]
MroI         1032  TCCGGA               T^CCGGA
NarI         2054  GGCGCC               GG^CGCC
NdeI         4263  CATATG               CA^TATG
PflFI         637  GACTGTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
SacII          48  CCGCGG               CCGC^GG
SapI          212  GAAGAGC              GCTCTTC(1/4)
SbfI         1540  CCTGCAGG             CCTGCA^GG
SfoI         2054  GGCGCC               GGC^GCC
SpeI         3260  ACTAGT               A^CTAGT
Sse8387I     1540  CCTGCAGG             CCTGCA^GG
Tth111I       637  GACTGTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
XhoII         790  GGATCT               [GA]^GATC[CT]
XmnI         3195  GAAGGTTTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   39 enzymes) #
###################################

AccII           3  CGCG                 CG^CG
               49  CGCG                
AflII         482  CTTAAG               C^TTAAG
             3644  CTTAAG              
AflIII       1326  ACATGT               A^C[GA][CT]GT
             4232  ACATGT              
AhdI         2505  GACTGGATGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
             2980  GACTGTATGTC         
AspEI        2505  GACTGGATGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
             2980  GACTGTATGTC         
BalI         1989  TGGCCA               TGG^CCA
             4197  TGGCCA              
BclI         1725  TGATCA               T^GATCA
             4581  TGATCA              
BfrI          482  CTTAAG               C^TTAAG
             3644  CTTAAG              
BlpI         2038  GCTGAGC              GC^T[ACGT]AGC
             4146  GCTTAGC             
Bpu1102I     2038  GCTGAGC              GC^T[ACGT]AGC
             4146  GCTTAGC             
BsaI         4174  GAGACC               GGTCTC(1/5)
              133  GGTCTC              
BsmFI        1738  GGGAC                GGGAC(10/14)
              142  GTCCC               
Bsp1407I     4515  TGTACA               T^GTACA
             4694  TGTACA              
BspLU11I     1326  ACATGT               A^CATGT
             4232  ACATGT              
BsrFI         630  ACCGGC               [GA]^CCGG[CT]
             2574  GCCGGT              
BsrGI        4515  TGTACA               T^GTACA
             4694  TGTACA              
BstUI           3  CGCG                 CG^CG
               49  CGCG                
CelII        2038  GCTGAGC              GC^T[ACGT]AGC
             4146  GCTTAGC             
CfoI            2  GCGC                 GCG^C
             2055  GCGC                
Cfr10I        630  ACCGGC               [GA]^CCGG[CT]
             2574  GCCGGT              
DrdI         2439  GACAGCACAGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
             2634  GACAGAGAAGTC        
Eam1105I     2505  GACTGGATGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
             2980  GACTGTATGTC         
EcoRI         320  GAATTC               G^AATTC
             3361  GAATTC              
EcoT14I        54  CCATGG               C^C[AT][AT]GG
             1551  CCATGG              
FbaI         1725  TGATCA               T^GATCA
             4581  TGATCA              
HhaI            2  GCGC                 GCG^C
             2055  GCGC                
HinP1I          2  GCGC                 G^CGC
             2055  GCGC                
MluNI        1989  TGGCCA               TGG^CCA
             4197  TGGCCA              
MscI         1989  TGGCCA               TGG^CCA
             4197  TGGCCA              
MvnI            3  CGCG                 CG^CG
               49  CGCG                
NcoI           54  CCATGG               C^CATGG
             1551  CCATGG              
PflMI        1647  CCAAGAGCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             2658  CCATTTCCTGG         
SexAI        1702  ACCAGGT              A^CC[AT]GGT
             1995  ACCTGGT             
SspBI        4515  TGTACA               T^GTACA
             4694  TGTACA              
StyI           54  CCATGG               C^C[AT][AT]GG
             1551  CCATGG              
TaqI          111  TCGA                 T^CGA
             1690  TCGA                
TthHB8I       111  TCGA                 T^CGA
             1690  TCGA                
Van91I       1647  CCAAGAGCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             2658  CCATTTCCTGG         
XbaI         3485  TCTAGA               T^CTAGA
             4053  TCTAGA              


#########################################
# 3 OR MORE CUT ENZYMES (  102 enzymes) #
#########################################

AciI        AcsI        AfaI        AluI        AlwI        
AlwNI       AocI        ApoI        AseI        AsnI        
AspHI       AvaII       BbsI        BbvI        BciVI       
BcnI        BfaI        BmyI        BpmI        BpuAI       
BsaJI       BseRI       BsgI        BsiHKAI     BsiYI       
BslI        BsmAI       Bsp1286I    BspHI       BspMI       
BsrI        BssKI       BstF5I      BstNI       BstXI       
Bsu36I      Cac8I       Cfr13I      DdeI        DpnII       
DraI        DraII       DsaI        EaeI        EarI        
Eco81I      EcoNI       EcoO109I    EcoRII      EcoT22I     
FauI        Fnu4HI      FokI        HaeIII      HapII       
HincII      HindII      HindIII     HinfI       HpaII       
HphI        ItaI        Ksp632I     MaeI        MaeII       
MaeIII      MboI        MboII       MnlI        MseI        
MslI        MspA1I      MspI        MvaI        MwoI        
NciI        NdeII       NlaIII      NlaIV       NsiI        
NspI        PleI        PpuMI       PshBI       PstI        
PvuII       RcaI        RsaI        Sau3AI      Sau96I      
ScrFI       SfaNI       SfcI        SmlI        SspI        
TfiI        Tru9I       TseI        Tsp45I      Tsp509I     
TspRI       XcmI