##################################
# NO CUT ENZYMES ( 65 enzymes) #
##################################
AatII Acc65I AclI AgeI Aor51HI
AscI AseI AsnI Asp718I AspI
AviII BglII BsiWI Bsp1407I BspLU11I
BsrFI BsrGI BssHII BstBI Cfr10I
CpoI DraIII DrdI Eco47III EcoRI
EcoRV FseI FspI KpnI KspI
MluI NaeI NdeI NgoMIV NheI
NotI NruI NspV PacI PflFI
PinAI PmeI PshAI PshBI Psp1406I
PvuI RsrII SacI SacII SalI
SapI SbfI ScaI SexAI SfiI
SfuI SgrAI SnaBI SpeI SphI
Sse8387I SspBI SspI SwaI Tth111I
###################################
# ONE CUT ENZYMES ( 57 enzymes) #
###################################
AccI 2119 GTATAC GT^[AC][GT]AC
AccII 60 CGCG CG^CG
AccIII 3982 TCCGGA T^CCGGA
AflII 1872 CTTAAG C^TTAAG
AocI 1675 CCTCAGG CC^T[ACGT]AGG
ApaI 3408 GGGCCC GGGCC^C
Asp700I 159 GAAGTGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BalI 268 TGGCCA TGG^CCA
BbeI 2859 GGCGCC GGCGC^C
BbrPI 2363 CACGTG CAC^GTG
BcgI 3963 CGATTCTTCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI 4686 TGATCA T^GATCA
BfrI 1872 CTTAAG C^TTAAG
BlpI 2261 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 2261 GCTGAGC GC^T[ACGT]AGC
BsaBI 4833 GATTCACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI 2943 GGTCTC GGTCTC(1/5)
BsaWI 3982 TCCGGA [AT]^CCGG[AT]
BseAI 3982 TCCGGA T^CCGGA
BsiEI 1253 CGGCCG CG[GA][CT]^CG
BsmBI 2127 CGTCTC CGTCTC(1/5)
BspDI 3485 ATCGAT AT^CGAT
BspEI 3982 TCCGGA T^CCGGA
BsrBI 1152 CCGCTC CCGCTC(-3/-3)
BssSI 1311 CTCGTG CACGAG(-5/-1)
Bst1107I 2119 GTATAC GTA^TAC
BstUI 60 CGCG CG^CG
BstZ17I 2119 GTATAC GTA^TAC
Bsu36I 1675 CCTCAGG CC^T[ACGT]AGG
CelII 2261 GCTGAGC GC^T[ACGT]AGC
ClaI 3485 ATCGAT AT^CGAT
DraI 3785 TTTAAA TTT^AAA
EagI 1253 CGGCCG C^GGCCG
EclXI 1253 CGGCCG C^GGCCG
Eco52I 1253 CGGCCG C^GGCCG
Eco81I 1675 CCTCAGG CC^T[ACGT]AGG
FbaI 4686 TGATCA T^GATCA
HpaI 2499 GTTAAC GTT^AAC
KasI 2859 GGCGCC G^GCGCC
MamI 4833 GATTCACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 253 CAATTG C^AATTG
MluNI 268 TGGCCA TGG^CCA
MroI 3982 TCCGGA T^CCGGA
MscI 268 TGGCCA TGG^CCA
MunI 253 CAATTG C^AATTG
MvnI 60 CGCG CG^CG
NarI 2859 GGCGCC GG^CGCC
NcoI 1066 CCATGG C^CATGG
PaeR7I 1800 CTCGAG C^TCGAG
PflMI 260 CCAGTTCATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI 2363 CACGTG CAC^GTG
PmlI 2363 CACGTG CAC^GTG
SfoI 2859 GGCGCC GGC^GCC
Van91I 260 CCAGTTCATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI 3732 TCTAGA T^CTAGA
XhoI 1800 CTCGAG C^TCGAG
XmnI 159 GAAGTGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 30 enzymes) #
###################################
AfaI 133 GTAC GT^AC
4254 GTAC
AhdI 3299 GACCCAAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
4365 GACAAGATGTC
Alw44I 1172 GTGCAC G^TGCAC
3714 GTGCAC
ApaLI 1172 GTGCAC G^TGCAC
3714 GTGCAC
AspEI 3299 GACCCAAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
4365 GACAAGATGTC
AvrII 1530 CCTAGG C^CTAGG
3266 CCTAGG
BamHI 3821 GGATCC G^GATCC
4388 GGATCC
BciVI 99 GGATAC GTATCC(6/5)
218 GGATAC
BglI 270 GCCACCAAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
2317 GCCCTCCAGGC
BlnI 1530 CCTAGG C^CTAGG
3266 CCTAGG
BsaAI 2344 TACGTG [CT]AC^GT[GA]
2363 CACGTG
BstAPI 2198 GCACCGCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
3771 GCAGGAACTGC
BstEII 3804 GGTCACC G^GT[ACGT]ACC
4648 GGTAACC
BstPI 3804 GGTCACC G^GT[ACGT]ACC
4648 GGTAACC
BstXI 379 CCAAGGGGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
568 CCAGACCTCTGG
Eam1105I 3299 GACCCAAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
4365 GACAAGATGTC
EcoNI 1898 CCTCAGTGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
4417 CCTATAGGAGG
EcoO65I 3804 GGTCACC G^GT[ACGT]ACC
4648 GGTAACC
EcoT22I 2247 ATGCAT ATGCA^T
4816 ATGCAT
HaeII 958 AGCGCC [GA]GCGC^[CT]
2859 GGCGCC
HincII 417 GTCAAC GT[CT]^[GA]AC
2499 GTTAAC
HindII 417 GTCAAC GT[CT]^[GA]AC
2499 GTTAAC
HindIII 303 AAGCTT A^AGCTT
4741 AAGCTT
NsiI 2247 ATGCAT ATGCA^T
4816 ATGCAT
RsaI 133 GTAC GT^AC
4254 GTAC
SmaI 1506 CCCGGG CCC^GGG
3550 CCCGGG
StuI 3475 AGGCCT AGG^CCT
4063 AGGCCT
XcmI 2391 CCAGTGCTGTCGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
4880 CCACACAAGTCTTGG
XmaCI 1506 CCCGGG C^CCGGG
3550 CCCGGG
XmaI 1506 CCCGGG C^CCGGG
3550 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 106 enzymes) #
#########################################
AciI AcsI AcyI AflIII AluI
AlwI AlwNI ApoI AspHI AvaI
AvaII BanI BanII BbsI BbvCI
BbvI BcnI BfaI BmyI BpmI
BpuAI BsaHI BsaJI BseRI BsgI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsmI BsoBI Bsp1286I BspHI BspMI
BsrDI BsrI BssKI BstF5I BstNI
BstYI Cac8I CfoI Cfr13I DdeI
DpnII DraII DsaI EaeI EarI
EcoO109I EcoRII EcoT14I FauI Fnu4HI
FokI HaeIII HapII HgaI HhaI
Hin1I HinP1I HinfI HpaII HphI
ItaI Ksp632I MaeI MaeII MaeIII
MboI MboII MflI MnlI MseI
MslI MspA1I MspI MvaI MwoI
NciI NdeII NlaIII NlaIV NspI
PleI PpuMI PstI PvuII RcaI
Sau3AI Sau96I ScrFI SfaNI SfcI
SmlI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
XhoII