################################## # NO CUT ENZYMES ( 103 enzymes) # ################################## AccII AccIII AclI AgeI AhdI Aor51HI ApaI AscI AspEI AspI AvaI BalI BamHI BbeI BbrPI BbvCI BcgI BclI BlpI Bpu1102I BsaAI BsaBI BsaI BsaWI BseAI BsiEI BsiWI BsmBI BsoBI BspEI BspHI BssHII BssSI Bst1107I BstEII BstPI BstUI BstXI BstZ17I CelII CpoI DraII DraIII DrdI EagI Eam1105I EclXI Eco47III Eco52I EcoO109I EcoO65I EcoT22I FauI FbaI FseI HaeII HpaI KasI KspI MamI MluI MluNI MroI MscI MvnI NaeI NarI NdeI NgoMIV NheI NotI NruI NsiI PacI PaeR7I PflFI PinAI PmaCI PmeI PmlI PpuMI PshAI Psp1406I PvuI RcaI RsrII SacII SalI SapI SbfI SexAI SfiI SfoI SgrAI SmaI SnaBI Sse8387I SwaI Tth111I XbaI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 54 enzymes) # ################################### AatII 3252 GACGTC GACGT^C Acc65I 1564 GGTACC G^GTACC AcyI 3252 GACGTC G[GA]^CG[CT]C AocI 1911 CCTTAGG CC^T[ACGT]AGG Asp718I 1564 GGTACC G^GTACC AvaII 2552 GGACC G^G[AT]CC AviII 1993 TGCGCA TGC^GCA AvrII 1760 CCTAGG C^CTAGG BciVI 1916 GGATAC GTATCC(6/5) BcnI 147 CCGGG CC^[GC]GG BglI 3468 GCCAAAAGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BlnI 1760 CCTAGG C^CTAGG BsaHI 3252 GACGTC G[GA]^CG[CT]C BsgI 4221 CTGCAC GTGCAG(16/14) Bsp1407I 5097 TGTACA T^GTACA BspDI 1738 ATCGAT AT^CGAT BspMI 2903 ACCTGC ACCTGC(4/8) BsrBI 4761 CCGCTC CCGCTC(-3/-3) BsrDI 3728 CATTGC GCAATG(2/0) BsrFI 4225 ACCGGC [GA]^CCGG[CT] BsrGI 5097 TGTACA T^GTACA BstBI 2529 TTCGAA TT^CGAA Bsu36I 1911 CCTTAGG CC^T[ACGT]AGG CfoI 1994 GCGC GCG^C Cfr10I 4225 ACCGGC [GA]^CCGG[CT] Cfr13I 2552 GGACC G^G[ACGT]CC ClaI 1738 ATCGAT AT^CGAT DsaI 3040 CCATGG C^C[GA][CT]GG EaeI 1143 TGGCCG [CT]^GGCC[GA] Eco81I 1911 CCTTAGG CC^T[ACGT]AGG EcoNI 3152 CCTAGAGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG FspI 1993 TGCGCA TGC^GCA HgaI 1631 GCGTC GACGC(5/10) HhaI 1994 GCGC GCG^C Hin1I 3252 GACGTC G[GA]^CG[CT]C HinP1I 1994 GCGC G^CGC HincII 637 GTTGAC GT[CT]^[GA]AC HindII 637 GTTGAC GT[CT]^[GA]AC KpnI 1564 GGTACC GGTAC^C MfeI 5027 CAATTG C^AATTG MunI 5027 CAATTG C^AATTG NciI 147 CCGGG CC^[GC]GG NcoI 3040 CCATGG C^CATGG NspV 2529 TTCGAA TT^CGAA PflMI 922 CCATTCATTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG Sau96I 2552 GGACC G^G[ACGT]CC ScaI 317 AGTACT AGT^ACT SfuI 2529 TTCGAA TT^CGAA SpeI 343 ACTAGT A^CTAGT SphI 726 GCATGC GCATG^C SspBI 5097 TGTACA T^GTACA StuI 4320 AGGCCT AGG^CCT Van91I 922 CCATTCATTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XcmI 5022 CCAGTCAATTGTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG ################################### # TWO CUT ENZYMES ( 13 enzymes) # ################################### AccI 1846 GTCTAC GT^[AC][GT]AC 2523 GTAGAC AflII 290 CTTAAG C^TTAAG 313 CTTAAG Alw44I 630 GTGCAC G^TGCAC 1587 GTGCAC ApaLI 630 GTGCAC G^TGCAC 1587 GTGCAC BanI 816 GGTGCC G^G[CT][GA]CC 1564 GGTACC BfrI 290 CTTAAG C^TTAAG 313 CTTAAG BsmFI 2551 GGGAC GGGAC(10/14) 4787 GTCCC BsmI 3239 GAATGC GAATGC(1/-1) 4268 GAATGC BstAPI 2817 GCAGCAGATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC 3723 GCACACATTGC HapII 147 CCGG C^CGG 4226 CCGG HindIII 513 AAGCTT A^AGCTT 903 AAGCTT HpaII 147 CCGG C^CGG 4226 CCGG MspI 147 CCGG C^CGG 4226 CCGG ######################################### # 3 OR MORE CUT ENZYMES ( 88 enzymes) # ######################################### AciI AcsI AfaI AflIII AluI AlwI AlwNI ApoI AseI AsnI Asp700I AspHI BanII BbsI BbvI BfaI BglII BmyI BpmI BpuAI BsaJI BseRI BsiHKAI BsiYI BslI BsmAI Bsp1286I BspLU11I BsrI BssKI BstF5I BstNI BstYI Cac8I DdeI DpnII DraI EarI EcoRI EcoRII EcoRV EcoT14I Fnu4HI FokI HaeIII HinfI HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MnlI MseI MslI MspA1I MvaI MwoI NdeII NlaIII NlaIV NspI PleI PshBI PstI PvuII RsaI SacI Sau3AI ScrFI SfaNI SfcI SmlI SspI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I XhoII XmnI