##################################
# NO CUT ENZYMES ( 103 enzymes) #
##################################
AccII AccIII AclI AgeI AhdI
Aor51HI ApaI AscI AspEI AspI
AvaI BalI BamHI BbeI BbrPI
BbvCI BcgI BclI BlpI Bpu1102I
BsaAI BsaBI BsaI BsaWI BseAI
BsiEI BsiWI BsmBI BsoBI BspEI
BspHI BssHII BssSI Bst1107I BstEII
BstPI BstUI BstXI BstZ17I CelII
CpoI DraII DraIII DrdI EagI
Eam1105I EclXI Eco47III Eco52I EcoO109I
EcoO65I EcoT22I FauI FbaI FseI
HaeII HpaI KasI KspI MamI
MluI MluNI MroI MscI MvnI
NaeI NarI NdeI NgoMIV NheI
NotI NruI NsiI PacI PaeR7I
PflFI PinAI PmaCI PmeI PmlI
PpuMI PshAI Psp1406I PvuI RcaI
RsrII SacII SalI SapI SbfI
SexAI SfiI SfoI SgrAI SmaI
SnaBI Sse8387I SwaI Tth111I XbaI
XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 54 enzymes) #
###################################
AatII 3252 GACGTC GACGT^C
Acc65I 1564 GGTACC G^GTACC
AcyI 3252 GACGTC G[GA]^CG[CT]C
AocI 1911 CCTTAGG CC^T[ACGT]AGG
Asp718I 1564 GGTACC G^GTACC
AvaII 2552 GGACC G^G[AT]CC
AviII 1993 TGCGCA TGC^GCA
AvrII 1760 CCTAGG C^CTAGG
BciVI 1916 GGATAC GTATCC(6/5)
BcnI 147 CCGGG CC^[GC]GG
BglI 3468 GCCAAAAGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlnI 1760 CCTAGG C^CTAGG
BsaHI 3252 GACGTC G[GA]^CG[CT]C
BsgI 4221 CTGCAC GTGCAG(16/14)
Bsp1407I 5097 TGTACA T^GTACA
BspDI 1738 ATCGAT AT^CGAT
BspMI 2903 ACCTGC ACCTGC(4/8)
BsrBI 4761 CCGCTC CCGCTC(-3/-3)
BsrDI 3728 CATTGC GCAATG(2/0)
BsrFI 4225 ACCGGC [GA]^CCGG[CT]
BsrGI 5097 TGTACA T^GTACA
BstBI 2529 TTCGAA TT^CGAA
Bsu36I 1911 CCTTAGG CC^T[ACGT]AGG
CfoI 1994 GCGC GCG^C
Cfr10I 4225 ACCGGC [GA]^CCGG[CT]
Cfr13I 2552 GGACC G^G[ACGT]CC
ClaI 1738 ATCGAT AT^CGAT
DsaI 3040 CCATGG C^C[GA][CT]GG
EaeI 1143 TGGCCG [CT]^GGCC[GA]
Eco81I 1911 CCTTAGG CC^T[ACGT]AGG
EcoNI 3152 CCTAGAGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
FspI 1993 TGCGCA TGC^GCA
HgaI 1631 GCGTC GACGC(5/10)
HhaI 1994 GCGC GCG^C
Hin1I 3252 GACGTC G[GA]^CG[CT]C
HinP1I 1994 GCGC G^CGC
HincII 637 GTTGAC GT[CT]^[GA]AC
HindII 637 GTTGAC GT[CT]^[GA]AC
KpnI 1564 GGTACC GGTAC^C
MfeI 5027 CAATTG C^AATTG
MunI 5027 CAATTG C^AATTG
NciI 147 CCGGG CC^[GC]GG
NcoI 3040 CCATGG C^CATGG
NspV 2529 TTCGAA TT^CGAA
PflMI 922 CCATTCATTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Sau96I 2552 GGACC G^G[ACGT]CC
ScaI 317 AGTACT AGT^ACT
SfuI 2529 TTCGAA TT^CGAA
SpeI 343 ACTAGT A^CTAGT
SphI 726 GCATGC GCATG^C
SspBI 5097 TGTACA T^GTACA
StuI 4320 AGGCCT AGG^CCT
Van91I 922 CCATTCATTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 5022 CCAGTCAATTGTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 13 enzymes) #
###################################
AccI 1846 GTCTAC GT^[AC][GT]AC
2523 GTAGAC
AflII 290 CTTAAG C^TTAAG
313 CTTAAG
Alw44I 630 GTGCAC G^TGCAC
1587 GTGCAC
ApaLI 630 GTGCAC G^TGCAC
1587 GTGCAC
BanI 816 GGTGCC G^G[CT][GA]CC
1564 GGTACC
BfrI 290 CTTAAG C^TTAAG
313 CTTAAG
BsmFI 2551 GGGAC GGGAC(10/14)
4787 GTCCC
BsmI 3239 GAATGC GAATGC(1/-1)
4268 GAATGC
BstAPI 2817 GCAGCAGATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
3723 GCACACATTGC
HapII 147 CCGG C^CGG
4226 CCGG
HindIII 513 AAGCTT A^AGCTT
903 AAGCTT
HpaII 147 CCGG C^CGG
4226 CCGG
MspI 147 CCGG C^CGG
4226 CCGG
#########################################
# 3 OR MORE CUT ENZYMES ( 88 enzymes) #
#########################################
AciI AcsI AfaI AflIII AluI
AlwI AlwNI ApoI AseI AsnI
Asp700I AspHI BanII BbsI BbvI
BfaI BglII BmyI BpmI BpuAI
BsaJI BseRI BsiHKAI BsiYI BslI
BsmAI Bsp1286I BspLU11I BsrI BssKI
BstF5I BstNI BstYI Cac8I DdeI
DpnII DraI EarI EcoRI EcoRII
EcoRV EcoT14I Fnu4HI FokI HaeIII
HinfI HphI ItaI Ksp632I MaeI
MaeII MaeIII MboI MboII MflI
MnlI MseI MslI MspA1I MvaI
MwoI NdeII NlaIII NlaIV NspI
PleI PshBI PstI PvuII RsaI
SacI Sau3AI ScrFI SfaNI SfcI
SmlI SspI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I XhoII XmnI