##################################
# NO CUT ENZYMES (  103 enzymes) #
##################################

AccII       AccIII      AclI        AgeI        AhdI        
Aor51HI     ApaI        AscI        AspEI       AspI        
AvaI        BalI        BamHI       BbeI        BbrPI       
BbvCI       BcgI        BclI        BlpI        Bpu1102I    
BsaAI       BsaBI       BsaI        BsaWI       BseAI       
BsiEI       BsiWI       BsmBI       BsoBI       BspEI       
BspHI       BssHII      BssSI       Bst1107I    BstEII      
BstPI       BstUI       BstXI       BstZ17I     CelII       
CpoI        DraII       DraIII      DrdI        EagI        
Eam1105I    EclXI       Eco47III    Eco52I      EcoO109I    
EcoO65I     EcoT22I     FauI        FbaI        FseI        
HaeII       HpaI        KasI        KspI        MamI        
MluI        MluNI       MroI        MscI        MvnI        
NaeI        NarI        NdeI        NgoMIV      NheI        
NotI        NruI        NsiI        PacI        PaeR7I      
PflFI       PinAI       PmaCI       PmeI        PmlI        
PpuMI       PshAI       Psp1406I    PvuI        RcaI        
RsrII       SacII       SalI        SapI        SbfI        
SexAI       SfiI        SfoI        SgrAI       SmaI        
SnaBI       Sse8387I    SwaI        Tth111I     XbaI        
XhoI        XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   54 enzymes) #
###################################

AatII        3252  GACGTC               GACGT^C
Acc65I       1564  GGTACC               G^GTACC
AcyI         3252  GACGTC               G[GA]^CG[CT]C
AocI         1911  CCTTAGG              CC^T[ACGT]AGG
Asp718I      1564  GGTACC               G^GTACC
AvaII        2552  GGACC                G^G[AT]CC
AviII        1993  TGCGCA               TGC^GCA
AvrII        1760  CCTAGG               C^CTAGG
BciVI        1916  GGATAC               GTATCC(6/5)
BcnI          147  CCGGG                CC^[GC]GG
BglI         3468  GCCAAAAGGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlnI         1760  CCTAGG               C^CTAGG
BsaHI        3252  GACGTC               G[GA]^CG[CT]C
BsgI         4221  CTGCAC               GTGCAG(16/14)
Bsp1407I     5097  TGTACA               T^GTACA
BspDI        1738  ATCGAT               AT^CGAT
BspMI        2903  ACCTGC               ACCTGC(4/8)
BsrBI        4761  CCGCTC               CCGCTC(-3/-3)
BsrDI        3728  CATTGC               GCAATG(2/0)
BsrFI        4225  ACCGGC               [GA]^CCGG[CT]
BsrGI        5097  TGTACA               T^GTACA
BstBI        2529  TTCGAA               TT^CGAA
Bsu36I       1911  CCTTAGG              CC^T[ACGT]AGG
CfoI         1994  GCGC                 GCG^C
Cfr10I       4225  ACCGGC               [GA]^CCGG[CT]
Cfr13I       2552  GGACC                G^G[ACGT]CC
ClaI         1738  ATCGAT               AT^CGAT
DsaI         3040  CCATGG               C^C[GA][CT]GG
EaeI         1143  TGGCCG               [CT]^GGCC[GA]
Eco81I       1911  CCTTAGG              CC^T[ACGT]AGG
EcoNI        3152  CCTAGAGAAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
FspI         1993  TGCGCA               TGC^GCA
HgaI         1631  GCGTC                GACGC(5/10)
HhaI         1994  GCGC                 GCG^C
Hin1I        3252  GACGTC               G[GA]^CG[CT]C
HinP1I       1994  GCGC                 G^CGC
HincII        637  GTTGAC               GT[CT]^[GA]AC
HindII        637  GTTGAC               GT[CT]^[GA]AC
KpnI         1564  GGTACC               GGTAC^C
MfeI         5027  CAATTG               C^AATTG
MunI         5027  CAATTG               C^AATTG
NciI          147  CCGGG                CC^[GC]GG
NcoI         3040  CCATGG               C^CATGG
NspV         2529  TTCGAA               TT^CGAA
PflMI         922  CCATTCATTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Sau96I       2552  GGACC                G^G[ACGT]CC
ScaI          317  AGTACT               AGT^ACT
SfuI         2529  TTCGAA               TT^CGAA
SpeI          343  ACTAGT               A^CTAGT
SphI          726  GCATGC               GCATG^C
SspBI        5097  TGTACA               T^GTACA
StuI         4320  AGGCCT               AGG^CCT
Van91I        922  CCATTCATTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI         5022  CCAGTCAATTGTTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG


###################################
# TWO CUT ENZYMES (   13 enzymes) #
###################################

AccI         1846  GTCTAC               GT^[AC][GT]AC
             2523  GTAGAC              
AflII         290  CTTAAG               C^TTAAG
              313  CTTAAG              
Alw44I        630  GTGCAC               G^TGCAC
             1587  GTGCAC              
ApaLI         630  GTGCAC               G^TGCAC
             1587  GTGCAC              
BanI          816  GGTGCC               G^G[CT][GA]CC
             1564  GGTACC              
BfrI          290  CTTAAG               C^TTAAG
              313  CTTAAG              
BsmFI        2551  GGGAC                GGGAC(10/14)
             4787  GTCCC               
BsmI         3239  GAATGC               GAATGC(1/-1)
             4268  GAATGC              
BstAPI       2817  GCAGCAGATGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
             3723  GCACACATTGC         
HapII         147  CCGG                 C^CGG
             4226  CCGG                
HindIII       513  AAGCTT               A^AGCTT
              903  AAGCTT              
HpaII         147  CCGG                 C^CGG
             4226  CCGG                
MspI          147  CCGG                 C^CGG
             4226  CCGG                


#########################################
# 3 OR MORE CUT ENZYMES (   88 enzymes) #
#########################################

AciI        AcsI        AfaI        AflIII      AluI        
AlwI        AlwNI       ApoI        AseI        AsnI        
Asp700I     AspHI       BanII       BbsI        BbvI        
BfaI        BglII       BmyI        BpmI        BpuAI       
BsaJI       BseRI       BsiHKAI     BsiYI       BslI        
BsmAI       Bsp1286I    BspLU11I    BsrI        BssKI       
BstF5I      BstNI       BstYI       Cac8I       DdeI        
DpnII       DraI        EarI        EcoRI       EcoRII      
EcoRV       EcoT14I     Fnu4HI      FokI        HaeIII      
HinfI       HphI        ItaI        Ksp632I     MaeI        
MaeII       MaeIII      MboI        MboII       MflI        
MnlI        MseI        MslI        MspA1I      MvaI        
MwoI        NdeII       NlaIII      NlaIV       NspI        
PleI        PshBI       PstI        PvuII       RsaI        
SacI        Sau3AI      ScrFI       SfaNI       SfcI        
SmlI        SspI        StyI        TaqI        TfiI        
Tru9I       TseI        Tsp45I      Tsp509I     TspRI       
TthHB8I     XhoII       XmnI