################################## # NO CUT ENZYMES ( 78 enzymes) # ################################## Acc65I AccIII AclI AgeI Alw44I Aor51HI ApaI ApaLI AscI Asp718I AviII BbeI BbrPI BcgI BsaAI BsaWI BseAI BsiEI BsiWI BsmBI BspDI BspEI BsrBI BssHII BssSI Bst1107I BstAPI BstBI BstZ17I ClaI CpoI DraIII DrdI EagI EclXI Eco47III Eco52I EcoT22I FseI FspI KasI KpnI KspI MluI MroI NaeI NarI NgoMIV NheI NotI NruI NsiI NspV PacI PaeR7I PinAI PmaCI PmeI PmlI PshAI Psp1406I PvuI RsrII SacII SalI SbfI SfiI SfoI SfuI SnaBI SpeI SphI Sse8387I SspI SwaI XbaI XcmI XhoI ################################### # ONE CUT ENZYMES ( 43 enzymes) # ################################### AatII 2353 GACGTC GACGT^C AcyI 2353 GACGTC G[GA]^CG[CT]C AflII 2695 CTTAAG C^TTAAG AocI 320 CCTGAGG CC^T[ACGT]AGG AseI 1481 ATTAAT AT^TAAT AsnI 1481 ATTAAT AT^TAAT AvaI 95 CCCGGG C^[CT]CG[GA]G BamHI 721 GGATCC G^GATCC BbvCI 33 GCTGAGG CCTCAGC(-5/-2) BciVI 4578 GGATAC GTATCC(6/5) BfrI 2695 CTTAAG C^TTAAG BglI 2184 GCCTAAGAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BsaHI 2353 GACGTC G[GA]^CG[CT]C BseRI 4671 CTCCTC GAGGAG(10/8) BsoBI 95 CCCGGG C^[CT]CG[GA]G BsrFI 149 GCCGGT [GA]^CCGG[CT] BstEII 862 GGTGACC G^GT[ACGT]ACC BstPI 862 GGTGACC G^GT[ACGT]ACC Bsu36I 320 CCTGAGG CC^T[ACGT]AGG Cfr10I 149 GCCGGT [GA]^CCGG[CT] Eco81I 320 CCTGAGG CC^T[ACGT]AGG EcoO65I 862 GGTGACC G^GT[ACGT]ACC EcoRI 2997 GAATTC G^AATTC EcoRV 4215 GATATC GAT^ATC FauI 175 GCGGG CCCGC(4/6) HaeII 188 AGCGCC [GA]GCGC^[CT] HgaI 382 GCGTC GACGC(5/10) Hin1I 2353 GACGTC G[GA]^CG[CT]C HindIII 844 AAGCTT A^AGCTT MfeI 1474 CAATTG C^AATTG MunI 1474 CAATTG C^AATTG NcoI 1944 CCATGG C^CATGG PflMI 5130 CCAACAAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PshBI 1481 ATTAAT AT^TAAT SapI 1166 GAAGAGC GCTCTTC(1/4) ScaI 2972 AGTACT AGT^ACT SexAI 5106 ACCTGGT A^CC[AT]GGT SgrAI 148 CGCCGGTG C[GA]^CCGG[CT]G SmaI 95 CCCGGG CCC^GGG StuI 2715 AGGCCT AGG^CCT Van91I 5130 CCAACAAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XmaCI 95 CCCGGG C^CCGGG XmaI 95 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 25 enzymes) # ################################### AccII 146 CGCG CG^CG 381 CGCG AhdI 992 GACTTACAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 1439 GACATTAAGTC Asp700I 1054 GAAGACTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 1271 GAAGAAGTTC AspEI 992 GACTTACAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 1439 GACATTAAGTC AspI 865 GACCATGTC GAC[ACGT]^[ACGT][ACGT]GTC 4550 GACTTTGTC BclI 2911 TGATCA T^GATCA 4110 TGATCA BglII 2489 AGATCT A^GATCT 3317 AGATCT BsaBI 3523 GATAGAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC 4579 GATACCCATC BsgI 1011 GTGCAG GTGCAG(16/14) 4319 GTGCAG BspHI 805 TCATGA T^CATGA 2357 TCATGA BsrDI 3752 CATTGC GCAATG(2/0) 2371 GCAATG BstUI 146 CGCG CG^CG 381 CGCG BstXI 2588 CCAAGTAGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 4812 CCAGAAACATGG DsaI 1728 CCACGG C^C[GA][CT]GG 1944 CCATGG Eam1105I 992 GACTTACAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 1439 GACATTAAGTC FbaI 2911 TGATCA T^GATCA 4110 TGATCA MaeII 1454 ACGT A^CGT 2354 ACGT MamI 3523 GATAGAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC 4579 GATACCCATC MvnI 146 CGCG CG^CG 381 CGCG NdeI 2609 CATATG CA^TATG 5241 CATATG PflFI 865 GACCATGTC GAC[ACGT]^[ACGT][ACGT]GTC 4550 GACTTTGTC PpuMI 598 AGGTCCT [GA]G^G[AT]CC[CT] 1113 AGGACCT RcaI 805 TCATGA T^CATGA 2357 TCATGA Tth111I 865 GACCATGTC GAC[ACGT]^[ACGT][ACGT]GTC 4550 GACTTTGTC XmnI 1054 GAAGACTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 1271 GAAGAAGTTC ######################################### # 3 OR MORE CUT ENZYMES ( 112 enzymes) # ######################################### AccI AciI AcsI AfaI AflIII AluI AlwI AlwNI ApoI AspHI AvaII AvrII BalI BanI BanII BbsI BbvI BcnI BfaI BlnI BlpI BmyI BpmI Bpu1102I BpuAI BsaI BsaJI BsiHKAI BsiYI BslI BsmAI BsmFI BsmI Bsp1286I Bsp1407I BspLU11I BspMI BsrGI BsrI BssKI BstF5I BstNI BstYI Cac8I CelII CfoI Cfr13I DdeI DpnII DraI DraII EaeI EarI EcoNI EcoO109I EcoRII EcoT14I Fnu4HI FokI HaeIII HapII HhaI HinP1I HincII HindII HinfI HpaI HpaII HphI ItaI Ksp632I MaeI MaeIII MboI MboII MflI MluNI MnlI MscI MseI MslI MspA1I MspI MvaI MwoI NciI NdeII NlaIII NlaIV NspI PleI PstI PvuII RsaI SacI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI SspBI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I XhoII