##################################
# NO CUT ENZYMES (   78 enzymes) #
##################################

Acc65I      AccIII      AclI        AgeI        Alw44I      
Aor51HI     ApaI        ApaLI       AscI        Asp718I     
AviII       BbeI        BbrPI       BcgI        BsaAI       
BsaWI       BseAI       BsiEI       BsiWI       BsmBI       
BspDI       BspEI       BsrBI       BssHII      BssSI       
Bst1107I    BstAPI      BstBI       BstZ17I     ClaI        
CpoI        DraIII      DrdI        EagI        EclXI       
Eco47III    Eco52I      EcoT22I     FseI        FspI        
KasI        KpnI        KspI        MluI        MroI        
NaeI        NarI        NgoMIV      NheI        NotI        
NruI        NsiI        NspV        PacI        PaeR7I      
PinAI       PmaCI       PmeI        PmlI        PshAI       
Psp1406I    PvuI        RsrII       SacII       SalI        
SbfI        SfiI        SfoI        SfuI        SnaBI       
SpeI        SphI        Sse8387I    SspI        SwaI        
XbaI        XcmI        XhoI        


###################################
# ONE CUT ENZYMES (   43 enzymes) #
###################################

AatII        2353  GACGTC               GACGT^C
AcyI         2353  GACGTC               G[GA]^CG[CT]C
AflII        2695  CTTAAG               C^TTAAG
AocI          320  CCTGAGG              CC^T[ACGT]AGG
AseI         1481  ATTAAT               AT^TAAT
AsnI         1481  ATTAAT               AT^TAAT
AvaI           95  CCCGGG               C^[CT]CG[GA]G
BamHI         721  GGATCC               G^GATCC
BbvCI          33  GCTGAGG              CCTCAGC(-5/-2)
BciVI        4578  GGATAC               GTATCC(6/5)
BfrI         2695  CTTAAG               C^TTAAG
BglI         2184  GCCTAAGAGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaHI        2353  GACGTC               G[GA]^CG[CT]C
BseRI        4671  CTCCTC               GAGGAG(10/8)
BsoBI          95  CCCGGG               C^[CT]CG[GA]G
BsrFI         149  GCCGGT               [GA]^CCGG[CT]
BstEII        862  GGTGACC              G^GT[ACGT]ACC
BstPI         862  GGTGACC              G^GT[ACGT]ACC
Bsu36I        320  CCTGAGG              CC^T[ACGT]AGG
Cfr10I        149  GCCGGT               [GA]^CCGG[CT]
Eco81I        320  CCTGAGG              CC^T[ACGT]AGG
EcoO65I       862  GGTGACC              G^GT[ACGT]ACC
EcoRI        2997  GAATTC               G^AATTC
EcoRV        4215  GATATC               GAT^ATC
FauI          175  GCGGG                CCCGC(4/6)
HaeII         188  AGCGCC               [GA]GCGC^[CT]
HgaI          382  GCGTC                GACGC(5/10)
Hin1I        2353  GACGTC               G[GA]^CG[CT]C
HindIII       844  AAGCTT               A^AGCTT
MfeI         1474  CAATTG               C^AATTG
MunI         1474  CAATTG               C^AATTG
NcoI         1944  CCATGG               C^CATGG
PflMI        5130  CCAACAAATGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PshBI        1481  ATTAAT               AT^TAAT
SapI         1166  GAAGAGC              GCTCTTC(1/4)
ScaI         2972  AGTACT               AGT^ACT
SexAI        5106  ACCTGGT              A^CC[AT]GGT
SgrAI         148  CGCCGGTG             C[GA]^CCGG[CT]G
SmaI           95  CCCGGG               CCC^GGG
StuI         2715  AGGCCT               AGG^CCT
Van91I       5130  CCAACAAATGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmaCI          95  CCCGGG               C^CCGGG
XmaI           95  CCCGGG               C^CCGGG


###################################
# TWO CUT ENZYMES (   25 enzymes) #
###################################

AccII         146  CGCG                 CG^CG
              381  CGCG                
AhdI          992  GACTTACAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
             1439  GACATTAAGTC         
Asp700I      1054  GAAGACTTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
             1271  GAAGAAGTTC          
AspEI         992  GACTTACAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
             1439  GACATTAAGTC         
AspI          865  GACCATGTC            GAC[ACGT]^[ACGT][ACGT]GTC
             4550  GACTTTGTC           
BclI         2911  TGATCA               T^GATCA
             4110  TGATCA              
BglII        2489  AGATCT               A^GATCT
             3317  AGATCT              
BsaBI        3523  GATAGAAATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
             4579  GATACCCATC          
BsgI         1011  GTGCAG               GTGCAG(16/14)
             4319  GTGCAG              
BspHI         805  TCATGA               T^CATGA
             2357  TCATGA              
BsrDI        3752  CATTGC               GCAATG(2/0)
             2371  GCAATG              
BstUI         146  CGCG                 CG^CG
              381  CGCG                
BstXI        2588  CCAAGTAGCTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             4812  CCAGAAACATGG        
DsaI         1728  CCACGG               C^C[GA][CT]GG
             1944  CCATGG              
Eam1105I      992  GACTTACAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
             1439  GACATTAAGTC         
FbaI         2911  TGATCA               T^GATCA
             4110  TGATCA              
MaeII        1454  ACGT                 A^CGT
             2354  ACGT                
MamI         3523  GATAGAAATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
             4579  GATACCCATC          
MvnI          146  CGCG                 CG^CG
              381  CGCG                
NdeI         2609  CATATG               CA^TATG
             5241  CATATG              
PflFI         865  GACCATGTC            GAC[ACGT]^[ACGT][ACGT]GTC
             4550  GACTTTGTC           
PpuMI         598  AGGTCCT              [GA]G^G[AT]CC[CT]
             1113  AGGACCT             
RcaI          805  TCATGA               T^CATGA
             2357  TCATGA              
Tth111I       865  GACCATGTC            GAC[ACGT]^[ACGT][ACGT]GTC
             4550  GACTTTGTC           
XmnI         1054  GAAGACTTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
             1271  GAAGAAGTTC          


#########################################
# 3 OR MORE CUT ENZYMES (  112 enzymes) #
#########################################

AccI        AciI        AcsI        AfaI        AflIII      
AluI        AlwI        AlwNI       ApoI        AspHI       
AvaII       AvrII       BalI        BanI        BanII       
BbsI        BbvI        BcnI        BfaI        BlnI        
BlpI        BmyI        BpmI        Bpu1102I    BpuAI       
BsaI        BsaJI       BsiHKAI     BsiYI       BslI        
BsmAI       BsmFI       BsmI        Bsp1286I    Bsp1407I    
BspLU11I    BspMI       BsrGI       BsrI        BssKI       
BstF5I      BstNI       BstYI       Cac8I       CelII       
CfoI        Cfr13I      DdeI        DpnII       DraI        
DraII       EaeI        EarI        EcoNI       EcoO109I    
EcoRII      EcoT14I     Fnu4HI      FokI        HaeIII      
HapII       HhaI        HinP1I      HincII      HindII      
HinfI       HpaI        HpaII       HphI        ItaI        
Ksp632I     MaeI        MaeIII      MboI        MboII       
MflI        MluNI       MnlI        MscI        MseI        
MslI        MspA1I      MspI        MvaI        MwoI        
NciI        NdeII       NlaIII      NlaIV       NspI        
PleI        PstI        PvuII       RsaI        SacI        
Sau3AI      Sau96I      ScrFI       SfaNI       SfcI        
SmlI        SspBI       StyI        TaqI        TfiI        
Tru9I       TseI        Tsp45I      Tsp509I     TspRI       
TthHB8I     XhoII