##################################
# NO CUT ENZYMES ( 69 enzymes) #
##################################
AccIII AclI AflII AgeI Aor51HI
AscI AseI AsnI BbeI BbrPI
BbsI BciVI BfrI BglII BpuAI
BsaAI BsaBI BseAI BsiWI BspEI
BspHI BssHII Bst1107I BstAPI BstBI
BstZ17I DrdI EagI EclXI Eco47III
Eco52I EcoRV EcoT22I FseI HpaI
KasI KspI MamI MfeI MluI
MroI MunI NarI NotI NruI
NsiI NspV PacI PaeR7I PinAI
PmaCI PmeI PmlI PshAI PshBI
Psp1406I RcaI SacII SalI SexAI
SfoI SfuI SgrAI SnaBI SpeI
SspI SwaI XbaI XhoI
###################################
# ONE CUT ENZYMES ( 45 enzymes) #
###################################
AatII 486 GACGTC GACGT^C
Acc65I 3512 GGTACC G^GTACC
AccII 2687 CGCG CG^CG
AflIII 2365 ACATGT A^C[GA][CT]GT
AhdI 2196 GACTACCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 4197 GTGCAC G^TGCAC
ApaLI 4197 GTGCAC G^TGCAC
Asp718I 3512 GGTACC G^GTACC
AspEI 2196 GACTACCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AviII 2737 TGCGCA TGC^GCA
BsiEI 38 CGATCG CG[GA][CT]^CG
BsmI 2396 GAATGC GAATGC(1/-1)
Bsp1407I 1966 TGTACA T^GTACA
BspDI 36 ATCGAT AT^CGAT
BspLU11I 2365 ACATGT A^CATGT
BsrFI 3422 GCCGGC [GA]^CCGG[CT]
BsrGI 1966 TGTACA T^GTACA
BstUI 2687 CGCG CG^CG
Cfr10I 3422 GCCGGC [GA]^CCGG[CT]
ClaI 36 ATCGAT AT^CGAT
CpoI 2524 CGGTCCG CG^G[AT]CCG
Eam1105I 2196 GACTACCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoRI 3977 GAATTC G^AATTC
FspI 2737 TGCGCA TGC^GCA
HincII 531 GTTGAC GT[CT]^[GA]AC
HindII 531 GTTGAC GT[CT]^[GA]AC
HindIII 1437 AAGCTT A^AGCTT
KpnI 3512 GGTACC GGTAC^C
MaeII 487 ACGT A^CGT
MvnI 2687 CGCG CG^CG
NaeI 3422 GCCGGC GCC^GGC
NdeI 4575 CATATG CA^TATG
NgoMIV 3422 GCCGGC G^CCGGC
NheI 1119 GCTAGC G^CTAGC
PvuI 38 CGATCG CGAT^CG
RsrII 2524 CGGTCCG CG^G[AT]CCG
SapI 2357 GCTCTTC GCTCTTC(1/4)
SbfI 3239 CCTGCAGG CCTGCA^GG
ScaI 1048 AGTACT AGT^ACT
SfiI 1082 GGCCAGCCTGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SphI 2575 GCATGC GCATG^C
Sse8387I 3239 CCTGCAGG CCTGCA^GG
SspBI 1966 TGTACA T^GTACA
StuI 519 AGGCCT AGG^CCT
XcmI 1886 CCACTTCTGGTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 23 enzymes) #
###################################
AccI 153 GTCTAC GT^[AC][GT]AC
2918 GTAGAC
AocI 2489 CCTCAGG CC^T[ACGT]AGG
4269 CCTCAGG
AvrII 3663 CCTAGG C^CTAGG
3800 CCTAGG
BamHI 1738 GGATCC G^GATCC
3445 GGATCC
BclI 1693 TGATCA T^GATCA
2003 TGATCA
BlnI 3663 CCTAGG C^CTAGG
3800 CCTAGG
BlpI 1065 GCTGAGC GC^T[ACGT]AGC
2675 GCTGAGC
Bpu1102I 1065 GCTGAGC GC^T[ACGT]AGC
2675 GCTGAGC
BsaI 1203 GAGACC GGTCTC(1/5)
3235 GAGACC
BsaWI 1251 ACCGGA [AT]^CCGG[AT]
2383 TCCGGT
BsgI 939 CTGCAC GTGCAG(16/14)
2250 GTGCAG
BssSI 129 CACGAG CACGAG(-5/-1)
2541 CTCGTG
BstEII 728 GGTCACC G^GT[ACGT]ACC
1595 GGTGACC
BstPI 728 GGTCACC G^GT[ACGT]ACC
1595 GGTGACC
Bsu36I 2489 CCTCAGG CC^T[ACGT]AGG
4269 CCTCAGG
CelII 1065 GCTGAGC GC^T[ACGT]AGC
2675 GCTGAGC
Eco81I 2489 CCTCAGG CC^T[ACGT]AGG
4269 CCTCAGG
EcoO65I 728 GGTCACC G^GT[ACGT]ACC
1595 GGTGACC
FbaI 1693 TGATCA T^GATCA
2003 TGATCA
NcoI 973 CCATGG C^CATGG
1879 CCATGG
SmaI 1378 CCCGGG CCC^GGG
2170 CCCGGG
XmaCI 1378 CCCGGG C^CCGGG
2170 CCCGGG
XmaI 1378 CCCGGG C^CCGGG
2170 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 121 enzymes) #
#########################################
AciI AcsI AcyI AfaI AluI
AlwI AlwNI ApaI ApoI Asp700I
AspHI AspI AvaI AvaII BalI
BanI BanII BbvCI BbvI BcgI
BcnI BfaI BglI BmyI BpmI
BsaHI BsaJI BseRI BsiHKAI BsiYI
BslI BsmAI BsmBI BsmFI BsoBI
Bsp1286I BspMI BsrBI BsrDI BsrI
BssKI BstF5I BstNI BstXI BstYI
Cac8I CfoI Cfr13I DdeI DpnII
DraI DraII DraIII DsaI EaeI
EarI EcoNI EcoO109I EcoRII EcoT14I
FauI Fnu4HI FokI HaeII HaeIII
HapII HgaI HhaI Hin1I HinP1I
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeIII MboI MboII MflI
MluNI MnlI MscI MseI MslI
MspA1I MspI MvaI MwoI NciI
NdeII NlaIII NlaIV NspI PflFI
PflMI PleI PpuMI PstI PvuII
RsaI SacI Sau3AI Sau96I ScrFI
SfaNI SfcI SmlI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI Tth111I TthHB8I Van91I XhoII
XmnI