##################################
# NO CUT ENZYMES ( 81 enzymes) #
##################################
AatII Acc65I AccIII AclI AflIII
AgeI AhdI Alw44I Aor51HI ApaLI
AscI Asp718I AspEI AviII BalI
BbrPI BcgI BclI BglI BsaAI
BseAI BsiEI BsiWI BspDI BspEI
BspLU11I BsrDI BsrFI BssHII BssSI
BstBI Cfr10I ClaI CpoI DraIII
DrdI EaeI EagI Eam1105I EclXI
Eco47III Eco52I EcoRV FbaI FseI
FspI HpaI KpnI KspI MluI
MluNI MroI MscI NaeI NgoMIV
NheI NotI NruI NspV PacI
PaeR7I PinAI PmaCI PmeI PmlI
Psp1406I PvuI RsrII SacII SapI
SbfI SfiI SfuI SgrAI SmaI
SnaBI SpeI Sse8387I XhoI XmaCI
XmaI
###################################
# ONE CUT ENZYMES ( 42 enzymes) #
###################################
AccII 4457 CGCG CG^CG
AflII 2085 CTTAAG C^TTAAG
ApaI 3687 GGGCCC GGGCC^C
AseI 2587 ATTAAT AT^TAAT
AsnI 2587 ATTAAT AT^TAAT
Asp700I 1877 GAATGTATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspI 2791 GACAAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvrII 2051 CCTAGG C^CTAGG
BamHI 464 GGATCC G^GATCC
BbvCI 4291 GCTGAGG CCTCAGC(-5/-2)
BfrI 2085 CTTAAG C^TTAAG
BlnI 2051 CCTAGG C^CTAGG
BsaWI 1630 ACCGGA [AT]^CCGG[AT]
BsmBI 3722 GAGACG CGTCTC(1/5)
Bsp1407I 4413 TGTACA T^GTACA
BspHI 2398 TCATGA T^CATGA
BsrBI 4434 CCGCTC CCGCTC(-3/-3)
BsrGI 4413 TGTACA T^GTACA
Bst1107I 1803 GTATAC GTA^TAC
BstEII 1455 GGTCACC G^GT[ACGT]ACC
BstPI 1455 GGTCACC G^GT[ACGT]ACC
BstUI 4457 CGCG CG^CG
BstXI 2552 CCAGTCTCTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstZ17I 1803 GTATAC GTA^TAC
EcoNI 3653 CCTTTGTTAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 1455 GGTCACC G^GT[ACGT]ACC
HindIII 598 AAGCTT A^AGCTT
MfeI 2576 CAATTG C^AATTG
MunI 2576 CAATTG C^AATTG
MvnI 4457 CGCG CG^CG
PflFI 2791 GACAAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PshAI 691 GACATATGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI 2587 ATTAAT AT^TAAT
RcaI 2398 TCATGA T^CATGA
SacI 2156 GAGCTC GAGCT^C
SalI 1602 GTCGAC G^TCGAC
SexAI 3183 ACCAGGT A^CC[AT]GGT
SphI 391 GCATGC GCATG^C
SspBI 4413 TGTACA T^GTACA
SwaI 1268 ATTTAAAT ATTT^AAAT
Tth111I 2791 GACAAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
XmnI 1877 GAATGTATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 30 enzymes) #
###################################
AocI 2803 CCTGAGG CC^T[ACGT]AGG
4153 CCTGAGG
AvaI 3682 CTCGGG C^[CT]CG[GA]G
4136 CCCGAG
BbeI 3463 GGCGCC GGCGC^C
3774 GGCGCC
BciVI 254 GGATAC GTATCC(6/5)
3229 GTATCC
BlpI 552 GCTCAGC GC^T[ACGT]AGC
4331 GCTCAGC
Bpu1102I 552 GCTCAGC GC^T[ACGT]AGC
4331 GCTCAGC
BsoBI 3682 CTCGGG C^[CT]CG[GA]G
4136 CCCGAG
Bsu36I 2803 CCTGAGG CC^T[ACGT]AGG
4153 CCTGAGG
CelII 552 GCTCAGC GC^T[ACGT]AGC
4331 GCTCAGC
DsaI 547 CCATGG C^C[GA][CT]GG
2785 CCATGG
Eco81I 2803 CCTGAGG CC^T[ACGT]AGG
4153 CCTGAGG
EcoRI 1320 GAATTC G^AATTC
2873 GAATTC
EcoT22I 1426 ATGCAT ATGCA^T
3172 ATGCAT
HgaI 1539 GACGC GACGC(5/10)
4064 GACGC
HincII 1250 GTCAAC GT[CT]^[GA]AC
1602 GTCGAC
HindII 1250 GTCAAC GT[CT]^[GA]AC
1602 GTCGAC
KasI 3463 GGCGCC G^GCGCC
3774 GGCGCC
MaeII 1513 ACGT A^CGT
1600 ACGT
MslI 1073 CACAGTAATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
2110 CATGCAAGTG
NarI 3463 GGCGCC GG^CGCC
3774 GGCGCC
NcoI 547 CCATGG C^CATGG
2785 CCATGG
NdeI 693 CATATG CA^TATG
2016 CATATG
NsiI 1426 ATGCAT ATGCA^T
3172 ATGCAT
NspI 391 GCATGC [GA]CATG^[CT]
2109 ACATGC
PleI 3301 GAGTC GAGTC(4/5)
4111 GAGTC
PvuII 2518 CAGCTG CAG^CTG
4462 CAGCTG
ScaI 2321 AGTACT AGT^ACT
2497 AGTACT
SfoI 3463 GGCGCC GGC^GCC
3774 GGCGCC
StuI 1612 AGGCCT AGG^CCT
3661 AGGCCT
XcmI 3900 CCAAGAAAGGTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
4199 CCATTAGCCAAGTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 105 enzymes) #
#########################################
AccI AciI AcsI AcyI AfaI
AluI AlwI AlwNI ApoI AspHI
AvaII BanI BanII BbsI BbvI
BcnI BfaI BglII BmyI BpmI
BpuAI BsaBI BsaHI BsaI BsaJI
BseRI BsgI BsiHKAI BsiYI BslI
BsmAI BsmFI BsmI Bsp1286I BspMI
BsrI BssKI BstAPI BstF5I BstNI
BstYI Cac8I CfoI Cfr13I DdeI
DpnII DraI DraII EarI EcoO109I
EcoRII EcoT14I FauI Fnu4HI FokI
HaeII HaeIII HapII HhaI Hin1I
HinP1I HinfI HpaII HphI ItaI
Ksp632I MaeI MaeIII MamI MboI
MboII MflI MnlI MseI MspA1I
MspI MvaI MwoI NciI NdeII
NlaIII NlaIV PflMI PpuMI PstI
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI SmlI SspI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I Van91I XbaI XhoII