##################################
# NO CUT ENZYMES ( 92 enzymes) #
##################################
Acc65I AccIII AclI AgeI Aor51HI
ApaI AscI Asp718I AviII AvrII
BalI BanI BbeI BbrPI BbsI
BbvCI BcgI BlnI BlpI Bpu1102I
BpuAI BsaAI BseAI BsiWI BsmBI
BspEI BspHI BspLU11I BsrBI BsrFI
BssHII BssSI BstBI CelII CfoI
Cfr10I CpoI DraIII DsaI EaeI
EagI EclXI Eco47III Eco52I EcoNI
EcoT14I FseI FspI HaeII HgaI
HhaI HinP1I KasI KpnI KspI
MfeI MluI MluNI MroI MscI
MunI NaeI NarI NcoI NgoMIV
NotI NruI NspV PacI PaeR7I
PinAI PmaCI PmeI PmlI Psp1406I
RcaI RsrII SacI SacII SbfI
SfiI SfoI SfuI SgrAI SnaBI
SphI Sse8387I StuI StyI SwaI
XcmI XhoI
###################################
# ONE CUT ENZYMES ( 49 enzymes) #
###################################
AatII 2356 GACGTC GACGT^C
AccII 93 CGCG CG^CG
AcyI 2356 GACGTC G[GA]^CG[CT]C
AflII 531 CTTAAG C^TTAAG
AflIII 4012 ACACGT A^C[GA][CT]GT
AhdI 2721 GACCACAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspEI 2721 GACCACAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 1842 GACCCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 189 CCCGGG C^[CT]CG[GA]G
BamHI 26 GGATCC G^GATCC
BciVI 4269 GGATAC GTATCC(6/5)
BfrI 531 CTTAAG C^TTAAG
BglI 2511 GCCAAAAAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 959 AGATCT A^GATCT
BsaHI 2356 GACGTC G[GA]^CG[CT]C
BsaWI 2076 ACCGGA [AT]^CCGG[AT]
BseRI 483 GAGGAG GAGGAG(10/8)
BsiEI 693 CGATCG CG[GA][CT]^CG
BsmI 839 GAATGC GAATGC(1/-1)
BsoBI 189 CCCGGG C^[CT]CG[GA]G
BspDI 2083 ATCGAT AT^CGAT
BstEII 1158 GGTGACC G^GT[ACGT]ACC
BstPI 1158 GGTGACC G^GT[ACGT]ACC
BstUI 93 CGCG CG^CG
BstXI 4775 CCAAGCTGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
ClaI 2083 ATCGAT AT^CGAT
DrdI 578 GACATATGAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eam1105I 2721 GACCACAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoO65I 1158 GGTGACC G^GT[ACGT]ACC
EcoRI 2983 GAATTC G^AATTC
EcoRV 1620 GATATC GAT^ATC
FauI 2331 CCCGC CCCGC(4/6)
Hin1I 2356 GACGTC G[GA]^CG[CT]C
MspA1I 2272 CAGCTG C[AC]G^C[GT]G
MvnI 93 CGCG CG^CG
NheI 1568 GCTAGC G^CTAGC
PflFI 1842 GACCCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 3401 CCAGCATTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PvuI 693 CGATCG CGAT^CG
PvuII 2272 CAGCTG CAG^CTG
SalI 2359 GTCGAC G^TCGAC
SapI 1435 GAAGAGC GCTCTTC(1/4)
SexAI 223 ACCAGGT A^CC[AT]GGT
SmaI 189 CCCGGG CCC^GGG
SpeI 434 ACTAGT A^CTAGT
Tth111I 1842 GACCCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 3401 CCAGCATTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmaCI 189 CCCGGG C^CCGGG
XmaI 189 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 12 enzymes) #
###################################
AciI 2332 CCGC CCGC(-3/-1)
3973 CCGC
Asp700I 1278 GAACAGTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
3764 GAATTTTTTC
BanII 2456 GAGCCC G[GA]GC[CT]^C
3578 GGGCTC
BsaI 253 GGTCTC GGTCTC(1/5)
1009 GGTCTC
BspMI 1564 ACCTGC ACCTGC(4/8)
2475 GCAGGT
BsrDI 1822 GCAATG GCAATG(2/0)
2383 GCAATG
HindIII 1236 AAGCTT A^AGCTT
4941 AAGCTT
NdeI 580 CATATG CA^TATG
3749 CATATG
PleI 585 GAGTC GAGTC(4/5)
833 GAGTC
PshAI 2362 GACCCAAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
4531 GACCTTTGTC
XbaI 365 TCTAGA T^CTAGA
3714 TCTAGA
XmnI 1278 GAACAGTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
3764 GAATTTTTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 105 enzymes) #
#########################################
AccI AcsI AfaI AluI Alw44I
AlwI AlwNI AocI ApaLI ApoI
AseI AsnI AspHI AvaII BbvI
BclI BcnI BfaI BmyI BpmI
BsaBI BsaJI BsgI BsiHKAI BsiYI
BslI BsmAI BsmFI Bsp1286I Bsp1407I
BsrGI BsrI BssKI Bst1107I BstAPI
BstF5I BstNI BstYI BstZ17I Bsu36I
Cac8I Cfr13I DdeI DpnII DraI
DraII EarI Eco81I EcoO109I EcoRII
EcoT22I FbaI Fnu4HI FokI HaeIII
HapII HincII HindII HinfI HpaI
HpaII HphI ItaI Ksp632I MaeI
MaeII MaeIII MamI MboI MboII
MflI MnlI MseI MslI MspI
MvaI MwoI NciI NdeII NlaIII
NlaIV NsiI NspI PpuMI PshBI
PstI RsaI Sau3AI Sau96I ScaI
ScrFI SfaNI SfcI SmlI SspBI
SspI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I XhoII