##################################
# NO CUT ENZYMES ( 89 enzymes) #
##################################
AatII Acc65I AccII AclI AcyI
AflII Alw44I AocI Aor51HI ApaI
ApaLI AscI Asp718I AspI AviII
BamHI BbeI BbrPI BbvCI BcgI
BfrI BglI BlpI Bpu1102I BsaHI
BsiEI BsiWI BspDI BsrBI BssHII
BssSI Bst1107I BstEII BstPI BstUI
BstZ17I Bsu36I CelII ClaI CpoI
DraIII EagI EclXI Eco47III Eco52I
Eco81I EcoNI EcoO65I EcoRV FauI
FseI FspI HaeII Hin1I KasI
KpnI KspI MluI MvnI NaeI
NarI NdeI NgoMIV NotI NruI
PacI PflFI PmaCI PmeI PmlI
Psp1406I PstI PvuI RsrII SacII
SalI SapI SbfI SexAI SfiI
SfoI SgrAI SmaI Sse8387I StuI
SwaI Tth111I XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 65 enzymes) #
###################################
AccI 782 GTCTAC GT^[AC][GT]AC
AccIII 2336 TCCGGA T^CCGGA
AciI 2055 GCGG CCGC(-3/-1)
AgeI 410 ACCGGT A^CCGGT
AhdI 2468 GACAGATTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspEI 2468 GACAGATTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspHI 2206 GAGCTC G[AT]GC[AT]^C
AvaI 1960 CTCGAG C^[CT]CG[GA]G
AvrII 534 CCTAGG C^CTAGG
BalI 315 TGGCCA TGG^CCA
BanI 1583 GGTGCC G^G[CT][GA]CC
BclI 389 TGATCA T^GATCA
BcnI 1315 CCCGG CC^[GC]GG
BlnI 534 CCTAGG C^CTAGG
BsaI 2575 GGTCTC GGTCTC(1/5)
BseAI 2336 TCCGGA T^CCGGA
BseRI 2170 CTCCTC GAGGAG(10/8)
BsgI 1807 CTGCAC GTGCAG(16/14)
BsiHKAI 2206 GAGCTC G[AT]GC[AT]^C
BsiYI 4049 CCAATATTTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI 4049 CCAATATTTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BsmI 2586 GAATGC GAATGC(1/-1)
BsoBI 1960 CTCGAG C^[CT]CG[GA]G
Bsp1407I 4483 TGTACA T^GTACA
BspEI 2336 TCCGGA T^CCGGA
BspHI 1246 TCATGA T^CATGA
BspMI 1844 GCAGGT ACCTGC(4/8)
BsrFI 410 ACCGGT [GA]^CCGG[CT]
BsrGI 4483 TGTACA T^GTACA
BstAPI 1844 GCAGGTGTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI 2131 TTCGAA TT^CGAA
CfoI 3979 GCGC GCG^C
Cfr10I 410 ACCGGT [GA]^CCGG[CT]
DrdI 1779 GACTGTGTAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI 3779 CCATGG C^C[GA][CT]GG
Eam1105I 2468 GACAGATTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoT22I 664 ATGCAT ATGCA^T
FbaI 389 TGATCA T^GATCA
HhaI 3979 GCGC GCG^C
HinP1I 3979 GCGC G^CGC
HindIII 3641 AAGCTT A^AGCTT
MluNI 315 TGGCCA TGG^CCA
MroI 2336 TCCGGA T^CCGGA
MscI 315 TGGCCA TGG^CCA
MspA1I 1030 CAGCTG C[AC]G^C[GT]G
NciI 1315 CCCGG CC^[GC]GG
NcoI 3779 CCATGG C^CATGG
NheI 444 GCTAGC G^CTAGC
NsiI 664 ATGCAT ATGCA^T
NspV 2131 TTCGAA TT^CGAA
PaeR7I 1960 CTCGAG C^TCGAG
PflMI 4049 CCAATATTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PinAI 410 ACCGGT A^CCGGT
PshAI 1064 GACCTTGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PvuII 1030 CAGCTG CAG^CTG
RcaI 1246 TCATGA T^CATGA
SacI 2206 GAGCTC GAGCT^C
ScaI 1287 AGTACT AGT^ACT
SfuI 2131 TTCGAA TT^CGAA
SnaBI 2422 TACGTA TAC^GTA
SphI 4407 GCATGC GCATG^C
SspBI 4483 TGTACA T^GTACA
Van91I 4049 CCAATATTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 1057 CCAGTAGGACCTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 1960 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 19 enzymes) #
###################################
AlwNI 2223 CAGTGTCTG CAG[ACGT][ACGT][ACGT]^CTG
2832 CAGTGACTG
BanII 1311 GAGCCC G[GA]GC[CT]^C
2206 GAGCTC
BmyI 1311 GAGCCC G[AGT]GC[ACT]^C
2206 GAGCTC
BpmI 2209 CTCCAG CTGGAG(16/14)
4827 CTGGAG
BsaBI 1077 GATCTACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
2118 GATGGAGATC
BsaWI 410 ACCGGT [AT]^CCGG[AT]
2336 TCCGGA
Bsp1286I 1311 GAGCCC G[AGT]GC[ACT]^C
2206 GAGCTC
BsrDI 2440 CATTGC GCAATG(2/0)
4330 CATTGC
BstXI 2032 CCATTATTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
4556 CCATTTTTGTGG
EaeI 315 TGGCCA [CT]^GGCC[GA]
2056 CGGCCA
HgaI 1803 GACGC GACGC(5/10)
2444 GCGTC
HincII 1970 GTTAAC GT[CT]^[GA]AC
3681 GTTAAC
HindII 1970 GTTAAC GT[CT]^[GA]AC
3681 GTTAAC
HpaI 1970 GTTAAC GTT^AAC
3681 GTTAAC
MamI 1077 GATCTACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
2118 GATGGAGATC
SfcI 3787 CTACAG C^T[GA][CT]AG
4846 CTACAG
SmlI 1960 CTCGAG C^T[CT][GA]AG
4307 CTTGAG
SpeI 4167 ACTAGT A^CTAGT
4799 ACTAGT
XbaI 3002 TCTAGA T^CTAGA
4235 TCTAGA
#########################################
# 3 OR MORE CUT ENZYMES ( 85 enzymes) #
#########################################
AcsI AfaI AflIII AluI AlwI
ApoI AseI AsnI Asp700I AvaII
BbsI BbvI BciVI BfaI BglII
BpuAI BsaAI BsaJI BsmAI BsmBI
BsmFI BspLU11I BsrI BssKI BstF5I
BstNI BstYI Cac8I Cfr13I DdeI
DpnII DraI DraII EarI EcoO109I
EcoRI EcoRII EcoT14I Fnu4HI FokI
HaeIII HapII HinfI HpaII HphI
ItaI Ksp632I MaeI MaeII MaeIII
MboI MboII MfeI MflI MnlI
MseI MslI MspI MunI MvaI
MwoI NdeII NlaIII NlaIV NspI
PleI PpuMI PshBI RsaI Sau3AI
Sau96I ScrFI SfaNI SspI StyI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I XhoII XmnI