##################################
# NO CUT ENZYMES ( 62 enzymes) #
##################################
AatII Acc65I AccIII AgeI Alw44I
AlwNI Aor51HI ApaLI AscI Asp718I
AviII BciVI BclI BglI BglII
BseAI BsiEI BsiWI BspDI BspEI
BspLU11I BsrFI BssHII BstAPI BstBI
Cfr10I ClaI CpoI DraIII EagI
EclXI Eco47III Eco52I EcoNI EcoT22I
FbaI FseI FspI KpnI KspI
MfeI MluI MroI MunI NaeI
NgoMIV NheI NotI NsiI NspV
PacI PinAI PvuI RsrII SacII
SbfI SfiI SfuI SgrAI SnaBI
Sse8387I SwaI
###################################
# ONE CUT ENZYMES ( 57 enzymes) #
###################################
AclI 531 AACGTT AA^CGTT
AhdI 980 GACGATGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AseI 143 ATTAAT AT^TAAT
AsnI 143 ATTAAT AT^TAAT
Asp700I 652 GAAGGCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI 980 GACGATGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 3594 GACGGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
BbeI 3632 GGCGCC GGCGC^C
BbrPI 3141 CACGTG CAC^GTG
BbsI 900 GTCTTC GAAGAC(2/6)
BbvCI 3116 CCTCAGC CCTCAGC(-5/-2)
BcgI 1666 GCAGCAGCCTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BpuAI 900 GTCTTC GAAGAC(2/6)
BsaBI 1201 GATGTAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI 3502 TCCGGT [AT]^CCGG[AT]
Bsp1407I 3203 TGTACA T^GTACA
BsrGI 3203 TGTACA T^GTACA
BssSI 4073 CACGAG CACGAG(-5/-1)
Bst1107I 4434 GTATAC GTA^TAC
BstZ17I 4434 GTATAC GTA^TAC
DrdI 20 GACTTTGAGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI 1448 CCATGG C^C[GA][CT]GG
Eam1105I 980 GACGATGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI 4780 GAAGAG CTCTTC(1/4)
EcoRV 1570 GATATC GAT^ATC
HaeII 3632 GGCGCC [GA]GCGC^[CT]
HpaI 1712 GTTAAC GTT^AAC
KasI 3632 GGCGCC G^GCGCC
Ksp632I 4780 GAAGAG CTCTTC(1/4)
MamI 1201 GATGTAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
NarI 3632 GGCGCC GG^CGCC
NcoI 1448 CCATGG C^CATGG
NdeI 4506 CATATG CA^TATG
NruI 1631 TCGCGA TCG^CGA
PaeR7I 1271 CTCGAG C^TCGAG
PflFI 3594 GACGGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PmaCI 3141 CACGTG CAC^GTG
PmeI 4547 GTTTAAAC GTTT^AAAC
PmlI 3141 CACGTG CAC^GTG
PshAI 4809 GACTAGTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI 143 ATTAAT AT^TAAT
Psp1406I 531 AACGTT AA^CGTT
PstI 1664 CTGCAG CTGCA^G
SacI 835 GAGCTC GAGCT^C
SalI 17 GTCGAC G^TCGAC
SapI 4780 GAAGAGC GCTCTTC(1/4)
ScaI 4226 AGTACT AGT^ACT
SfoI 3632 GGCGCC GGC^GCC
SmaI 2030 CCCGGG CCC^GGG
SpeI 4810 ACTAGT A^CTAGT
SphI 4795 GCATGC GCATG^C
SspBI 3203 TGTACA T^GTACA
Tth111I 3594 GACGGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
XhoI 1271 CTCGAG C^TCGAG
XmaCI 2030 CCCGGG C^CCGGG
XmaI 2030 CCCGGG C^CCGGG
XmnI 652 GAAGGCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 31 enzymes) #
###################################
AcyI 1617 GGCGTC G[GA]^CG[CT]C
3632 GGCGCC
AflIII 2271 ACACGT A^C[GA][CT]GT
3142 ACGTGT
AocI 245 CCTGAGG CC^T[ACGT]AGG
1359 CCTCAGG
AvrII 1401 CCTAGG C^CTAGG
3832 CCTAGG
BamHI 1461 GGATCC G^GATCC
4409 GGATCC
BlnI 1401 CCTAGG C^CTAGG
3832 CCTAGG
BlpI 3605 GCTCAGC GC^T[ACGT]AGC
3917 GCTCAGC
Bpu1102I 3605 GCTCAGC GC^T[ACGT]AGC
3917 GCTCAGC
BsaAI 2272 CACGTA [CT]AC^GT[GA]
3141 CACGTG
BsaHI 1617 GGCGTC G[GA]^CG[CT]C
3632 GGCGCC
BsaI 525 GAGACC GGTCTC(1/5)
884 GAGACC
BsgI 1643 GTGCAG GTGCAG(16/14)
2940 GTGCAG
BsmI 1290 GAATGC GAATGC(1/-1)
4785 GCATTC
BspHI 2538 TCATGA T^CATGA
3925 TCATGA
BsrBI 3642 CCGCTC CCGCTC(-3/-3)
1491 GAGCGG
BsrDI 3056 CATTGC GCAATG(2/0)
4857 CATTGC
Bsu36I 245 CCTGAGG CC^T[ACGT]AGG
1359 CCTCAGG
CelII 3605 GCTCAGC GC^T[ACGT]AGC
3917 GCTCAGC
Eco81I 245 CCTGAGG CC^T[ACGT]AGG
1359 CCTCAGG
EcoRI 2473 GAATTC G^AATTC
4849 GAATTC
FauI 889 CCCGC CCCGC(4/6)
3002 GCGGG
Hin1I 1617 GGCGTC G[GA]^CG[CT]C
3632 GGCGCC
PflMI 1581 CCAGTCTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3658 CCATCTCCTGG
PpuMI 1971 AGGACCT [GA]G^G[AT]CC[CT]
2366 AGGACCC
RcaI 2538 TCATGA T^CATGA
3925 TCATGA
SexAI 758 ACCTGGT A^CC[AT]GGT
1376 ACCTGGT
SspI 1297 AATATT AAT^ATT
4381 AATATT
StuI 654 AGGCCT AGG^CCT
3851 AGGCCT
Van91I 1581 CCAGTCTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3658 CCATCTCCTGG
XbaI 2410 TCTAGA T^CTAGA
4221 TCTAGA
XcmI 1448 CCATGGAATTTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1949 CCAGACCAGTGCTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 108 enzymes) #
#########################################
AccI AccII AciI AcsI AfaI
AflII AluI AlwI ApaI ApoI
AspHI AvaI AvaII BalI BanI
BanII BbvI BcnI BfaI BfrI
BmyI BpmI BsaJI BseRI BsiHKAI
BsiYI BslI BsmAI BsmBI BsmFI
BsoBI Bsp1286I BspMI BsrI BssKI
BstEII BstF5I BstNI BstPI BstUI
BstXI BstYI Cac8I CfoI Cfr13I
DdeI DpnII DraI DraII EaeI
EcoO109I EcoO65I EcoRII EcoT14I Fnu4HI
FokI HaeIII HapII HgaI HhaI
HinP1I HincII HindII HindIII HinfI
HpaII HphI ItaI MaeI MaeII
MaeIII MboI MboII MflI MluNI
MnlI MscI MseI MslI MspA1I
MspI MvaI MvnI MwoI NciI
NdeII NlaIII NlaIV NspI PleI
PvuII RsaI Sau3AI Sau96I ScrFI
SfaNI SfcI SmlI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I XhoII