##################################
# NO CUT ENZYMES ( 77 enzymes) #
##################################
AatII AccIII AclI AgeI AhdI
Alw44I Aor51HI ApaLI AscI AseI
AsnI AspEI BciVI BclI BsaBI
BsaWI BseAI BsiEI BsiWI BsmI
Bsp1407I BspDI BspEI BspHI BspLU11I
BsrDI BsrGI BssHII Bst1107I BstZ17I
ClaI CpoI DraI DrdI EagI
Eam1105I EclXI Eco47III Eco52I EcoRI
EcoRV EcoT22I FbaI FseI HgaI
HindIII KspI MamI MfeI MluI
MroI MunI NaeI NdeI NgoMIV
NotI NsiI PacI PinAI PmeI
PshAI PshBI Psp1406I PvuI RcaI
RsrII SacII SalI SapI SbfI
SgrAI SnaBI SpeI Sse8387I SspBI
SwaI XbaI
###################################
# ONE CUT ENZYMES ( 34 enzymes) #
###################################
AccI 3722 GTAGAC GT^[AC][GT]AC
AflII 2582 CTTAAG C^TTAAG
Asp700I 4647 GAAGTACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspI 2144 GACACCGTC GAC[ACGT]^[ACGT][ACGT]GTC
AviII 1902 TGCGCA TGC^GCA
BamHI 706 GGATCC G^GATCC
BbvCI 1307 CCTCAGC CCTCAGC(-5/-2)
BcgI 1515 CGAGCTCAGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfrI 2582 CTTAAG C^TTAAG
BsaI 849 GAGACC GGTCTC(1/5)
BsrFI 500 GCCGGT [GA]^CCGG[CT]
BstBI 4468 TTCGAA TT^CGAA
BstEII 395 GGTCACC G^GT[ACGT]ACC
BstPI 395 GGTCACC G^GT[ACGT]ACC
Cfr10I 500 GCCGGT [GA]^CCGG[CT]
EarI 3147 CTCTTC CTCTTC(1/4)
EcoO65I 395 GGTCACC G^GT[ACGT]ACC
FspI 1902 TGCGCA TGC^GCA
HpaI 3317 GTTAAC GTT^AAC
Ksp632I 3147 CTCTTC CTCTTC(1/4)
NruI 2333 TCGCGA TCG^CGA
NspV 4468 TTCGAA TT^CGAA
PaeR7I 4 CTCGAG C^TCGAG
PflFI 2144 GACACCGTC GAC[ACGT]^[ACGT][ACGT]GTC
SexAI 65 ACCAGGT A^CC[AT]GGT
SfuI 4468 TTCGAA TT^CGAA
SmaI 28 CCCGGG CCC^GGG
SspI 4460 AATATT AAT^ATT
Tth111I 2144 GACACCGTC GAC[ACGT]^[ACGT][ACGT]GTC
XcmI 2181 CCACACCTTATGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 4 CTCGAG C^TCGAG
XmaCI 28 CCCGGG C^CCGGG
XmaI 28 CCCGGG C^CCGGG
XmnI 4647 GAAGTACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 30 enzymes) #
###################################
Acc65I 2029 GGTACC G^GTACC
2825 GGTACC
AccII 163 CGCG CG^CG
2334 CGCG
AflIII 1975 ACGTGT A^C[GA][CT]GT
3475 ACACGT
Asp718I 2029 GGTACC G^GTACC
2825 GGTACC
AvrII 45 CCTAGG C^CTAGG
2908 CCTAGG
BbrPI 1974 CACGTG CAC^GTG
3476 CACGTG
BglII 2199 AGATCT A^GATCT
4581 AGATCT
BlnI 45 CCTAGG C^CTAGG
2908 CCTAGG
BlpI 3623 GCTGAGC GC^T[ACGT]AGC
3900 GCTGAGC
Bpu1102I 3623 GCTGAGC GC^T[ACGT]AGC
3900 GCTGAGC
BsaAI 1974 CACGTG [CT]AC^GT[GA]
3476 CACGTG
BsmBI 206 GAGACG CGTCTC(1/5)
3282 GAGACG
BsrBI 911 GAGCGG CCGCTC(-3/-3)
2114 GAGCGG
BstUI 163 CGCG CG^CG
2334 CGCG
CelII 3623 GCTGAGC GC^T[ACGT]AGC
3900 GCTGAGC
DraIII 64 CACCAGGTG CAC[ACGT][ACGT][ACGT]^GTG
4016 CACTGGGTG
EcoNI 1156 CCTGAGCAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
3971 CCTTAGGGAGG
HincII 3219 GTTGAC GT[CT]^[GA]AC
3317 GTTAAC
HindII 3219 GTTGAC GT[CT]^[GA]AC
3317 GTTAAC
KpnI 2029 GGTACC GGTAC^C
2825 GGTACC
MvnI 163 CGCG CG^CG
2334 CGCG
NheI 872 GCTAGC G^CTAGC
3896 GCTAGC
NspI 378 GCATGC [GA]CATG^[CT]
2298 GCATGC
PflMI 1032 CCAAGGCTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3117 CCAGAGTCTGG
PmaCI 1974 CACGTG CAC^GTG
3476 CACGTG
PmlI 1974 CACGTG CAC^GTG
3476 CACGTG
SacI 1373 GAGCTC GAGCT^C
1516 GAGCTC
SphI 378 GCATGC GCATG^C
2298 GCATGC
TfiI 3481 GATTC G^A[AT]TC
3594 GAATC
Van91I 1032 CCAAGGCTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3117 CCAGAGTCTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 117 enzymes) #
#########################################
AciI AcsI AcyI AfaI AluI
AlwI AlwNI AocI ApaI ApoI
AspHI AvaI AvaII BalI BanI
BanII BbeI BbsI BbvI BcnI
BfaI BglI BmyI BpmI BpuAI
BsaHI BsaJI BseRI BsgI BsiHKAI
BsiYI BslI BsmAI BsmFI BsoBI
Bsp1286I BspMI BsrI BssKI BssSI
BstAPI BstF5I BstNI BstXI BstYI
Bsu36I Cac8I CfoI Cfr13I DdeI
DpnII DraII DsaI EaeI Eco81I
EcoO109I EcoRII EcoT14I FauI Fnu4HI
FokI HaeII HaeIII HapII HhaI
Hin1I HinP1I HinfI HpaII HphI
ItaI KasI MaeI MaeII MaeIII
MboI MboII MflI MluNI MnlI
MscI MseI MslI MspA1I MspI
MvaI MwoI NarI NciI NcoI
NdeII NlaIII NlaIV PleI PpuMI
PstI PvuII RsaI Sau3AI Sau96I
ScaI ScrFI SfaNI SfcI SfiI
SfoI SmlI StuI StyI TaqI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I XhoII