##################################
# NO CUT ENZYMES (   68 enzymes) #
##################################

Acc65I      AccIII      AclI        AflII       AgeI        
AhdI        Alw44I      Aor51HI     ApaLI       AscI        
AseI        AsnI        Asp718I     AspEI       AspI        
AviII       BbrPI       BciVI       BfrI        BseAI       
BsiEI       BsiWI       BspDI       BspEI       BspHI       
BssHII      Bst1107I    BstZ17I     ClaI        EagI        
Eam1105I    EclXI       Eco47III    Eco52I      EcoRV       
FseI        FspI        HindIII     HpaI        KpnI        
MluI        MroI        NotI        NruI        PacI        
PaeR7I      PflFI       PinAI       PmaCI       PmeI        
PmlI        PshBI       Psp1406I    PvuI        RcaI        
SalI        SbfI        SfiI        SgrAI       SmlI        
SnaBI       SpeI        SphI        Sse8387I    SspI        
SwaI        Tth111I     XhoI        


###################################
# ONE CUT ENZYMES (   46 enzymes) #
###################################

AocI         3539  CCTGAGG              CC^T[ACGT]AGG
Asp700I      4228  GAACTAATTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvrII        3855  CCTAGG               C^CTAGG
BamHI         393  GGATCC               G^GATCC
BbeI            8  GGCGCC               GGCGC^C
BcgI         1335  GCAAGATTATCG         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI         2472  TGATCA               T^GATCA
BlnI         3855  CCTAGG               C^CTAGG
BlpI         1846  GCTCAGC              GC^T[ACGT]AGC
Bpu1102I     1846  GCTCAGC              GC^T[ACGT]AGC
BsaBI        1186  GATCTTCATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI        2671  ACCGGA               [AT]^CCGG[AT]
BsmBI        2032  CGTCTC               CGTCTC(1/5)
Bsp1407I     4517  TGTACA               T^GTACA
BsrGI        4517  TGTACA               T^GTACA
BssSI        1362  CTCGTG               CACGAG(-5/-1)
BstBI         972  TTCGAA               TT^CGAA
BstEII       4580  GGTGACC              G^GT[ACGT]ACC
BstPI        4580  GGTGACC              G^GT[ACGT]ACC
Bsu36I       3539  CCTGAGG              CC^T[ACGT]AGG
CelII        1846  GCTCAGC              GC^T[ACGT]AGC
CpoI          138  CGGTCCG              CG^G[AT]CCG
DraI         4243  TTTAAA               TTT^AAA
Eco81I       3539  CCTGAGG              CC^T[ACGT]AGG
EcoO65I      4580  GGTGACC              G^GT[ACGT]ACC
EcoRI        4156  GAATTC               G^AATTC
FbaI         2472  TGATCA               T^GATCA
KasI            8  GGCGCC               G^GCGCC
KspI         1862  CCGCGG               CCGC^GG
MamI         1186  GATCTTCATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI         3637  CAATTG               C^AATTG
MunI         3637  CAATTG               C^AATTG
NaeI         3683  GCCGGC               GCC^GGC
NarI            8  GGCGCC               GG^CGCC
NdeI         4634  CATATG               CA^TATG
NgoMIV       3683  GCCGGC               G^CCGGC
NheI         1675  GCTAGC               G^CTAGC
NspV          972  TTCGAA               TT^CGAA
RsrII         138  CGGTCCG              CG^G[AT]CCG
SacII        1862  CCGCGG               CCGC^GG
ScaI          438  AGTACT               AGT^ACT
SexAI        2819  ACCTGGT              A^CC[AT]GGT
SfoI            8  GGCGCC               GGC^GCC
SfuI          972  TTCGAA               TT^CGAA
SspBI        4517  TGTACA               T^GTACA
XmnI         4228  GAACTAATTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   17 enzymes) #
###################################

AatII         593  GACGTC               GACGT^C
             1123  GACGTC              
AccI         4125  GTCTAC               GT^[AC][GT]AC
             4483  GTCTAC              
AcsI         4156  GAATTC               [GA]^AATT[CT]
             4248  AAATTC              
ApoI         4156  GAATTC               [GA]^AATT[CT]
             4248  AAATTC              
BbsI          215  GAAGAC               GAAGAC(2/6)
              328  GAAGAC              
BpuAI         215  GAAGAC               GAAGAC(2/6)
              328  GAAGAC              
BsaI         1254  GAGACC               GGTCTC(1/5)
             1295  GAGACC              
BsgI          254  GTGCAG               GTGCAG(16/14)
              457  GTGCAG              
BsrBI        4047  CCGCTC               CCGCTC(-3/-3)
             3883  GAGCGG              
BsrDI        4394  CATTGC               GCAATG(2/0)
             1893  GCAATG              
DraIII        248  CACAGCGTG            CAC[ACGT][ACGT][ACGT]^GTG
             2146  CACCCAGTG           
DrdI         4267  GACCTTGCTGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
             4366  GACCGCAGTGTC        
EcoNI        2366  CCTTACACAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
             3275  CCTCTTGCAGG         
EcoT22I      3742  ATGCAT               ATGCA^T
             4456  ATGCAT              
HaeII           8  GGCGCC               [GA]GCGC^[CT]
             3105  AGCGCC              
NsiI         3742  ATGCAT               ATGCA^T
             4456  ATGCAT              
XcmI           12  CCAATGCCCAGGTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
             2826  CCAGCGAGGAGATGG     


#########################################
# 3 OR MORE CUT ENZYMES (  127 enzymes) #
#########################################

AccII       AciI        AcyI        AfaI        AflIII      
AluI        AlwI        AlwNI       ApaI        AspHI       
AvaI        AvaII       BalI        BanI        BanII       
BbvCI       BbvI        BcnI        BfaI        BglI        
BglII       BmyI        BpmI        BsaAI       BsaHI       
BsaJI       BseRI       BsiHKAI     BsiYI       BslI        
BsmAI       BsmFI       BsmI        BsoBI       Bsp1286I    
BspLU11I    BspMI       BsrFI       BsrI        BssKI       
BstAPI      BstF5I      BstNI       BstUI       BstXI       
BstYI       Cac8I       CfoI        Cfr10I      Cfr13I      
DdeI        DpnII       DraII       DsaI        EaeI        
EarI        EcoO109I    EcoRII      EcoT14I     FauI        
Fnu4HI      FokI        HaeIII      HapII       HgaI        
HhaI        Hin1I       HinP1I      HincII      HindII      
HinfI       HpaII       HphI        ItaI        Ksp632I     
MaeI        MaeII       MaeIII      MboI        MboII       
MflI        MluNI       MnlI        MscI        MseI        
MslI        MspA1I      MspI        MvaI        MvnI        
MwoI        NciI        NcoI        NdeII       NlaIII      
NlaIV       NspI        PflMI       PleI        PpuMI       
PshAI       PstI        PvuII       RsaI        SacI        
SapI        Sau3AI      Sau96I      ScrFI       SfaNI       
SfcI        SmaI        StuI        StyI        TaqI        
TfiI        Tru9I       TseI        Tsp45I      Tsp509I     
TspRI       TthHB8I     Van91I      XbaI        XhoII       
XmaCI       XmaI