################################## # NO CUT ENZYMES ( 68 enzymes) # ################################## Acc65I AccIII AclI AflII AgeI AhdI Alw44I Aor51HI ApaLI AscI AseI AsnI Asp718I AspEI AspI AviII BbrPI BciVI BfrI BseAI BsiEI BsiWI BspDI BspEI BspHI BssHII Bst1107I BstZ17I ClaI EagI Eam1105I EclXI Eco47III Eco52I EcoRV FseI FspI HindIII HpaI KpnI MluI MroI NotI NruI PacI PaeR7I PflFI PinAI PmaCI PmeI PmlI PshBI Psp1406I PvuI RcaI SalI SbfI SfiI SgrAI SmlI SnaBI SpeI SphI Sse8387I SspI SwaI Tth111I XhoI ################################### # ONE CUT ENZYMES ( 46 enzymes) # ################################### AocI 3539 CCTGAGG CC^T[ACGT]AGG Asp700I 4228 GAACTAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AvrII 3855 CCTAGG C^CTAGG BamHI 393 GGATCC G^GATCC BbeI 8 GGCGCC GGCGC^C BcgI 1335 GCAAGATTATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BclI 2472 TGATCA T^GATCA BlnI 3855 CCTAGG C^CTAGG BlpI 1846 GCTCAGC GC^T[ACGT]AGC Bpu1102I 1846 GCTCAGC GC^T[ACGT]AGC BsaBI 1186 GATCTTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsaWI 2671 ACCGGA [AT]^CCGG[AT] BsmBI 2032 CGTCTC CGTCTC(1/5) Bsp1407I 4517 TGTACA T^GTACA BsrGI 4517 TGTACA T^GTACA BssSI 1362 CTCGTG CACGAG(-5/-1) BstBI 972 TTCGAA TT^CGAA BstEII 4580 GGTGACC G^GT[ACGT]ACC BstPI 4580 GGTGACC G^GT[ACGT]ACC Bsu36I 3539 CCTGAGG CC^T[ACGT]AGG CelII 1846 GCTCAGC GC^T[ACGT]AGC CpoI 138 CGGTCCG CG^G[AT]CCG DraI 4243 TTTAAA TTT^AAA Eco81I 3539 CCTGAGG CC^T[ACGT]AGG EcoO65I 4580 GGTGACC G^GT[ACGT]ACC EcoRI 4156 GAATTC G^AATTC FbaI 2472 TGATCA T^GATCA KasI 8 GGCGCC G^GCGCC KspI 1862 CCGCGG CCGC^GG MamI 1186 GATCTTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MfeI 3637 CAATTG C^AATTG MunI 3637 CAATTG C^AATTG NaeI 3683 GCCGGC GCC^GGC NarI 8 GGCGCC GG^CGCC NdeI 4634 CATATG CA^TATG NgoMIV 3683 GCCGGC G^CCGGC NheI 1675 GCTAGC G^CTAGC NspV 972 TTCGAA TT^CGAA RsrII 138 CGGTCCG CG^G[AT]CCG SacII 1862 CCGCGG CCGC^GG ScaI 438 AGTACT AGT^ACT SexAI 2819 ACCTGGT A^CC[AT]GGT SfoI 8 GGCGCC GGC^GCC SfuI 972 TTCGAA TT^CGAA SspBI 4517 TGTACA T^GTACA XmnI 4228 GAACTAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 17 enzymes) # ################################### AatII 593 GACGTC GACGT^C 1123 GACGTC AccI 4125 GTCTAC GT^[AC][GT]AC 4483 GTCTAC AcsI 4156 GAATTC [GA]^AATT[CT] 4248 AAATTC ApoI 4156 GAATTC [GA]^AATT[CT] 4248 AAATTC BbsI 215 GAAGAC GAAGAC(2/6) 328 GAAGAC BpuAI 215 GAAGAC GAAGAC(2/6) 328 GAAGAC BsaI 1254 GAGACC GGTCTC(1/5) 1295 GAGACC BsgI 254 GTGCAG GTGCAG(16/14) 457 GTGCAG BsrBI 4047 CCGCTC CCGCTC(-3/-3) 3883 GAGCGG BsrDI 4394 CATTGC GCAATG(2/0) 1893 GCAATG DraIII 248 CACAGCGTG CAC[ACGT][ACGT][ACGT]^GTG 2146 CACCCAGTG DrdI 4267 GACCTTGCTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 4366 GACCGCAGTGTC EcoNI 2366 CCTTACACAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG 3275 CCTCTTGCAGG EcoT22I 3742 ATGCAT ATGCA^T 4456 ATGCAT HaeII 8 GGCGCC [GA]GCGC^[CT] 3105 AGCGCC NsiI 3742 ATGCAT ATGCA^T 4456 ATGCAT XcmI 12 CCAATGCCCAGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 2826 CCAGCGAGGAGATGG ######################################### # 3 OR MORE CUT ENZYMES ( 127 enzymes) # ######################################### AccII AciI AcyI AfaI AflIII AluI AlwI AlwNI ApaI AspHI AvaI AvaII BalI BanI BanII BbvCI BbvI BcnI BfaI BglI BglII BmyI BpmI BsaAI BsaHI BsaJI BseRI BsiHKAI BsiYI BslI BsmAI BsmFI BsmI BsoBI Bsp1286I BspLU11I BspMI BsrFI BsrI BssKI BstAPI BstF5I BstNI BstUI BstXI BstYI Cac8I CfoI Cfr10I Cfr13I DdeI DpnII DraII DsaI EaeI EarI EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeIII HapII HgaI HhaI Hin1I HinP1I HincII HindII HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MluNI MnlI MscI MseI MslI MspA1I MspI MvaI MvnI MwoI NciI NcoI NdeII NlaIII NlaIV NspI PflMI PleI PpuMI PshAI PstI PvuII RsaI SacI SapI Sau3AI Sau96I ScrFI SfaNI SfcI SmaI StuI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I Van91I XbaI XhoII XmaCI XmaI