##################################
# NO CUT ENZYMES (   63 enzymes) #
##################################

AccI        AccIII      AclI        AgeI        Aor51HI     
AscI        AseI        AsnI        BamHI       BciVI       
BclI        BsaBI       BsaWI       BseAI       BsiEI       
BsiWI       BspDI       BspEI       BsrBI       BssSI       
Bst1107I    BstBI       BstZ17I     ClaI        CpoI        
EagI        EarI        EclXI       Eco47III    Eco52I      
EcoRV       FbaI        FseI        HincII      HindII      
HpaI        Ksp632I     KspI        MamI        MluI        
MroI        NotI        NruI        NspV        PacI        
PinAI       PmeI        PshAI       PshBI       Psp1406I    
PvuI        PvuII       RsrII       SacII       SalI        
SapI        SfiI        SfuI        SgrAI       SnaBI       
SpeI        SwaI        XbaI        


###################################
# ONE CUT ENZYMES (   60 enzymes) #
###################################

AatII        1588  GACGTC               GACGT^C
Acc65I        556  GGTACC               G^GTACC
AflII        3704  CTTAAG               C^TTAAG
AhdI         1828  GACAGAACGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I       1969  GTGCAC               G^TGCAC
ApaLI        1969  GTGCAC               G^TGCAC
Asp700I      1140  GAACACCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I       556  GGTACC               G^GTACC
AspEI        1828  GACAGAACGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI         3111  GACTCTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
AviII        1658  TGCGCA               TGC^GCA
AvrII        2693  CCTAGG               C^CTAGG
BbeI         1636  GGCGCC               GGCGC^C
BbrPI         153  CACGTG               CAC^GTG
BcgI          148  CGATGCACGTGC         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfrI         3704  CTTAAG               C^TTAAG
BglII        3567  AGATCT               A^GATCT
BlnI         2693  CCTAGG               C^CTAGG
BlpI          739  GCTGAGC              GC^T[ACGT]AGC
Bpu1102I      739  GCTGAGC              GC^T[ACGT]AGC
BsaI         3775  GAGACC               GGTCTC(1/5)
BsmBI        1288  GAGACG               CGTCTC(1/5)
BsmI         3276  GCATTC               GAATGC(1/-1)
BsrDI         427  GCAATG               GCAATG(2/0)
BssHII          6  GCGCGC               G^CGCGC
CelII         739  GCTGAGC              GC^T[ACGT]AGC
DraIII       1558  CACATAGTG            CAC[ACGT][ACGT][ACGT]^GTG
DrdI          856  GACAAGCACGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eam1105I     1828  GACAGAACGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoT22I      3342  ATGCAT               ATGCA^T
FspI         1658  TGCGCA               TGC^GCA
KasI         1636  GGCGCC               G^GCGCC
KpnI          556  GGTACC               GGTAC^C
MfeI         3246  CAATTG               C^AATTG
MslI          792  CACATTAGTG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MunI         3246  CAATTG               C^AATTG
NaeI         2080  GCCGGC               GCC^GGC
NarI         1636  GGCGCC               GG^CGCC
NcoI          191  CCATGG               C^CATGG
NdeI         2075  CATATG               CA^TATG
NgoMIV       2080  GCCGGC               G^CCGGC
NheI         1476  GCTAGC               G^CTAGC
NsiI         3342  ATGCAT               ATGCA^T
PaeR7I       1330  CTCGAG               C^TCGAG
PflFI        3111  GACTCTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
PmaCI         153  CACGTG               CAC^GTG
PmlI          153  CACGTG               CAC^GTG
SacI          376  GAGCTC               GAGCT^C
SbfI         1017  CCTGCAGG             CCTGCA^GG
ScaI         1196  AGTACT               AGT^ACT
SfoI         1636  GGCGCC               GGC^GCC
SmaI         3044  CCCGGG               CCC^GGG
SphI          650  GCATGC               GCATG^C
Sse8387I     1017  CCTGCAGG             CCTGCA^GG
SspI         3989  AATATT               AAT^ATT
Tth111I      3111  GACTCTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
XhoI         1330  CTCGAG               C^TCGAG
XmaCI        3044  CCCGGG               C^CCGGG
XmaI         3044  CCCGGG               C^CCGGG
XmnI         1140  GAACACCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   27 enzymes) #
###################################

AcyI         1588  GACGTC               G[GA]^CG[CT]C
             1636  GGCGCC              
AocI         2903  CCTGAGG              CC^T[ACGT]AGG
             4344  CCTAAGG             
ApaI         1037  GGGCCC               GGGCC^C
             1237  GGGCCC              
BbsI          853  GAAGAC               GAAGAC(2/6)
             2415  GTCTTC              
BglI          679  GCCTTCCTGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
             3646  GCCGCAGTGGC         
BpuAI         853  GAAGAC               GAAGAC(2/6)
             2415  GTCTTC              
BsaAI         153  CACGTG               [CT]AC^GT[GA]
             3206  CACGTA              
BsaHI        1588  GACGTC               G[GA]^CG[CT]C
             1636  GGCGCC              
Bsp1407I     3160  TGTACA               T^GTACA
             4387  TGTACA              
BspLU11I     3163  ACATGT               A^CATGT
             4043  ACATGT              
BsrFI         294  GCCGGT               [GA]^CCGG[CT]
             2080  GCCGGC              
BsrGI        3160  TGTACA               T^GTACA
             4387  TGTACA              
BstAPI        726  GCAGGAGCTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
             1705  GCATGGACTGC         
BstYI        2366  AGATCC               [GA]^GATC[CT]
             3567  AGATCT              
Bsu36I       2903  CCTGAGG              CC^T[ACGT]AGG
             4344  CCTAAGG             
Cfr10I        294  GCCGGT               [GA]^CCGG[CT]
             2080  GCCGGC              
DsaI          191  CCATGG               C^C[GA][CT]GG
             2385  CCACGG              
Eco81I       2903  CCTGAGG              CC^T[ACGT]AGG
             4344  CCTAAGG             
EcoNI         705  CCTCTCACAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
             1269  CCTGCAAAAGG         
EcoRI        2134  GAATTC               G^AATTC
             2914  GAATTC              
HgaI         1290  GACGC                GACGC(5/10)
              927  GCGTC               
Hin1I        1588  GACGTC               G[GA]^CG[CT]C
             1636  GGCGCC              
HindIII      2527  AAGCTT               A^AGCTT
             4338  AAGCTT              
MflI         2366  AGATCC               [GA]^GATC[CT]
             3567  AGATCT              
MspA1I         58  CAGCGG               C[AC]G^C[GT]G
              642  CCGCTG              
SspBI        3160  TGTACA               T^GTACA
             4387  TGTACA              
XhoII        2366  AGATCC               [GA]^GATC[CT]
             3567  AGATCT              


#########################################
# 3 OR MORE CUT ENZYMES (  108 enzymes) #
#########################################

AccII       AciI        AcsI        AfaI        AflIII      
AluI        AlwI        AlwNI       ApoI        AspHI       
AvaI        AvaII       BalI        BanI        BanII       
BbvCI       BbvI        BcnI        BfaI        BmyI        
BpmI        BsaJI       BseRI       BsgI        BsiHKAI     
BsiYI       BslI        BsmAI       BsmFI       BsoBI       
Bsp1286I    BspHI       BspMI       BsrI        BssKI       
BstEII      BstF5I      BstNI       BstPI       BstUI       
BstXI       Cac8I       CfoI        Cfr13I      DdeI        
DpnII       DraI        DraII       EaeI        EcoO109I    
EcoO65I     EcoRII      EcoT14I     FauI        Fnu4HI      
FokI        HaeII       HaeIII      HapII       HhaI        
HinP1I      HinfI       HpaII       HphI        ItaI        
MaeI        MaeII       MaeIII      MboI        MboII       
MluNI       MnlI        MscI        MseI        MspI        
MvaI        MvnI        MwoI        NciI        NdeII       
NlaIII      NlaIV       NspI        PflMI       PleI        
PpuMI       PstI        RcaI        RsaI        Sau3AI      
Sau96I      ScrFI       SexAI       SfaNI       SfcI        
SmlI        StuI        StyI        TaqI        TfiI        
Tru9I       TseI        Tsp45I      Tsp509I     TspRI       
TthHB8I     Van91I      XcmI