##################################
# NO CUT ENZYMES (   59 enzymes) #
##################################

Acc65I      AccI        AclI        AgeI        Aor51HI     
AscI        Asp700I     Asp718I     AspI        AviII       
BclI        BglII       BsaBI       BsiWI       BspDI       
BspHI       BssHII      BssSI       Bst1107I    BstBI       
BstZ17I     ClaI        CpoI        DrdI        Eco47III    
EcoRV       FbaI        FspI        HincII      HindII      
HindIII     HpaI        KpnI        MamI        MfeI        
MluI        MunI        NheI        NotI        NspV        
PacI        PaeR7I      PflFI       PinAI       PmeI        
PshAI       Psp1406I    PvuI        RcaI        RsrII       
SalI        SexAI       SfuI        SnaBI       SpeI        
SwaI        Tth111I     XhoI        XmnI        


###################################
# ONE CUT ENZYMES (   38 enzymes) #
###################################

AatII        2519  GACGTC               GACGT^C
AccIII        264  TCCGGA               T^CCGGA
AhdI         2889  GACCCACGGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AseI         5594  ATTAAT               AT^TAAT
AsnI         5594  ATTAAT               AT^TAAT
AspEI        2889  GACCCACGGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BalI         4938  TGGCCA               TGG^CCA
BbrPI        4590  CACGTG               CAC^GTG
BciVI         257  GGATAC               GTATCC(6/5)
BsaAI        4590  CACGTG               [CT]AC^GT[GA]
BseAI         264  TCCGGA               T^CCGGA
BsmI         3993  GCATTC               GAATGC(1/-1)
Bsp1407I     1762  TGTACA               T^GTACA
BspEI         264  TCCGGA               T^CCGGA
BspLU11I     1759  ACATGT               A^CATGT
BsrGI        1762  TGTACA               T^GTACA
BstEII        489  GGTCACC              G^GT[ACGT]ACC
BstPI         489  GGTCACC              G^GT[ACGT]ACC
Eam1105I     2889  GACCCACGGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoO65I       489  GGTCACC              G^GT[ACGT]ACC
EcoRI        5838  GAATTC               G^AATTC
EcoT22I      1920  ATGCAT               ATGCA^T
FseI         2286  GGCCGGCC             GGCCGG^CC
MluNI        4938  TGGCCA               TGG^CCA
MroI          264  TCCGGA               T^CCGGA
MscI         4938  TGGCCA               TGG^CCA
NdeI          520  CATATG               CA^TATG
NruI          804  TCGCGA               TCG^CGA
NsiI         1920  ATGCAT               ATGCA^T
PmaCI        4590  CACGTG               CAC^GTG
PmlI         4590  CACGTG               CAC^GTG
PshBI        5594  ATTAAT               AT^TAAT
SbfI         4984  CCTGCAGG             CCTGCA^GG
SfiI         2031  GGCCCTGGCGGCC        GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SgrAI        2303  CGCCGGTG             C[GA]^CCGG[CT]G
Sse8387I     4984  CCTGCAGG             CCTGCA^GG
SspBI        1762  TGTACA               T^GTACA
StuI         5242  AGGCCT               AGG^CCT


###################################
# TWO CUT ENZYMES (   14 enzymes) #
###################################

AflII        4146  CTTAAG               C^TTAAG
             5081  CTTAAG              
AflIII       1759  ACATGT               A^C[GA][CT]GT
             4589  ACACGT              
Alw44I       3777  GTGCAC               G^TGCAC
             4449  GTGCAC              
ApaLI        3777  GTGCAC               G^TGCAC
             4449  GTGCAC              
BamHI        2353  GGATCC               G^GATCC
             4880  GGATCC              
BcgI         1302  CGAGAACTGTGC         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
             1225  GCAAGTACTTCG        
BfrI         4146  CTTAAG               C^TTAAG
             5081  CTTAAG              
BsrDI        5351  CATTGC               GCAATG(2/0)
             3565  GCAATG              
HgaI          141  GACGC                GACGC(5/10)
             3015  GACGC               
NcoI         2026  CCATGG               C^CATGG
             4398  CCATGG              
SacI         1464  GAGCTC               GAGCT^C
             3575  GAGCTC              
SphI          554  GCATGC               GCATG^C
             4561  GCATGC              
SspI         5751  AATATT               AAT^ATT
             5807  AATATT              
XbaI         4435  TCTAGA               T^CTAGA
             4615  TCTAGA              


#########################################
# 3 OR MORE CUT ENZYMES (  147 enzymes) #
#########################################

AccII       AciI        AcsI        AcyI        AfaI        
AluI        AlwI        AlwNI       AocI        ApaI        
ApoI        AspHI       AvaI        AvaII       AvrII       
BanI        BanII       BbeI        BbsI        BbvCI       
BbvI        BcnI        BfaI        BglI        BlnI        
BlpI        BmyI        BpmI        Bpu1102I    BpuAI       
BsaHI       BsaI        BsaJI       BsaWI       BseRI       
BsgI        BsiEI       BsiHKAI     BsiYI       BslI        
BsmAI       BsmBI       BsmFI       BsoBI       Bsp1286I    
BspMI       BsrBI       BsrFI       BsrI        BssKI       
BstAPI      BstF5I      BstNI       BstUI       BstXI       
BstYI       Bsu36I      Cac8I       CelII       CfoI        
Cfr10I      Cfr13I      DdeI        DpnII       DraI        
DraII       DraIII      DsaI        EaeI        EagI        
EarI        EclXI       Eco52I      Eco81I      EcoNI       
EcoO109I    EcoRII      EcoT14I     FauI        Fnu4HI      
FokI        HaeII       HaeIII      HapII       HhaI        
Hin1I       HinP1I      HinfI       HpaII       HphI        
ItaI        KasI        Ksp632I     KspI        MaeI        
MaeII       MaeIII      MboI        MboII       MflI        
MnlI        MseI        MslI        MspA1I      MspI        
MvaI        MvnI        MwoI        NaeI        NarI        
NciI        NdeII       NgoMIV      NlaIII      NlaIV       
NspI        PflMI       PleI        PpuMI       PstI        
PvuII       RsaI        SacII       SapI        Sau3AI      
Sau96I      ScaI        ScrFI       SfaNI       SfcI        
SfoI        SmaI        SmlI        StyI        TaqI        
TfiI        Tru9I       TseI        Tsp45I      Tsp509I     
TspRI       TthHB8I     Van91I      XcmI        XhoII       
XmaCI       XmaI