################################## # NO CUT ENZYMES ( 59 enzymes) # ################################## Acc65I AccI AclI AgeI Aor51HI AscI Asp700I Asp718I AspI AviII BclI BglII BsaBI BsiWI BspDI BspHI BssHII BssSI Bst1107I BstBI BstZ17I ClaI CpoI DrdI Eco47III EcoRV FbaI FspI HincII HindII HindIII HpaI KpnI MamI MfeI MluI MunI NheI NotI NspV PacI PaeR7I PflFI PinAI PmeI PshAI Psp1406I PvuI RcaI RsrII SalI SexAI SfuI SnaBI SpeI SwaI Tth111I XhoI XmnI ################################### # ONE CUT ENZYMES ( 38 enzymes) # ################################### AatII 2519 GACGTC GACGT^C AccIII 264 TCCGGA T^CCGGA AhdI 2889 GACCCACGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AseI 5594 ATTAAT AT^TAAT AsnI 5594 ATTAAT AT^TAAT AspEI 2889 GACCCACGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC BalI 4938 TGGCCA TGG^CCA BbrPI 4590 CACGTG CAC^GTG BciVI 257 GGATAC GTATCC(6/5) BsaAI 4590 CACGTG [CT]AC^GT[GA] BseAI 264 TCCGGA T^CCGGA BsmI 3993 GCATTC GAATGC(1/-1) Bsp1407I 1762 TGTACA T^GTACA BspEI 264 TCCGGA T^CCGGA BspLU11I 1759 ACATGT A^CATGT BsrGI 1762 TGTACA T^GTACA BstEII 489 GGTCACC G^GT[ACGT]ACC BstPI 489 GGTCACC G^GT[ACGT]ACC Eam1105I 2889 GACCCACGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EcoO65I 489 GGTCACC G^GT[ACGT]ACC EcoRI 5838 GAATTC G^AATTC EcoT22I 1920 ATGCAT ATGCA^T FseI 2286 GGCCGGCC GGCCGG^CC MluNI 4938 TGGCCA TGG^CCA MroI 264 TCCGGA T^CCGGA MscI 4938 TGGCCA TGG^CCA NdeI 520 CATATG CA^TATG NruI 804 TCGCGA TCG^CGA NsiI 1920 ATGCAT ATGCA^T PmaCI 4590 CACGTG CAC^GTG PmlI 4590 CACGTG CAC^GTG PshBI 5594 ATTAAT AT^TAAT SbfI 4984 CCTGCAGG CCTGCA^GG SfiI 2031 GGCCCTGGCGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC SgrAI 2303 CGCCGGTG C[GA]^CCGG[CT]G Sse8387I 4984 CCTGCAGG CCTGCA^GG SspBI 1762 TGTACA T^GTACA StuI 5242 AGGCCT AGG^CCT ################################### # TWO CUT ENZYMES ( 14 enzymes) # ################################### AflII 4146 CTTAAG C^TTAAG 5081 CTTAAG AflIII 1759 ACATGT A^C[GA][CT]GT 4589 ACACGT Alw44I 3777 GTGCAC G^TGCAC 4449 GTGCAC ApaLI 3777 GTGCAC G^TGCAC 4449 GTGCAC BamHI 2353 GGATCC G^GATCC 4880 GGATCC BcgI 1302 CGAGAACTGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) 1225 GCAAGTACTTCG BfrI 4146 CTTAAG C^TTAAG 5081 CTTAAG BsrDI 5351 CATTGC GCAATG(2/0) 3565 GCAATG HgaI 141 GACGC GACGC(5/10) 3015 GACGC NcoI 2026 CCATGG C^CATGG 4398 CCATGG SacI 1464 GAGCTC GAGCT^C 3575 GAGCTC SphI 554 GCATGC GCATG^C 4561 GCATGC SspI 5751 AATATT AAT^ATT 5807 AATATT XbaI 4435 TCTAGA T^CTAGA 4615 TCTAGA ######################################### # 3 OR MORE CUT ENZYMES ( 147 enzymes) # ######################################### AccII AciI AcsI AcyI AfaI AluI AlwI AlwNI AocI ApaI ApoI AspHI AvaI AvaII AvrII BanI BanII BbeI BbsI BbvCI BbvI BcnI BfaI BglI BlnI BlpI BmyI BpmI Bpu1102I BpuAI BsaHI BsaI BsaJI BsaWI BseRI BsgI BsiEI BsiHKAI BsiYI BslI BsmAI BsmBI BsmFI BsoBI Bsp1286I BspMI BsrBI BsrFI BsrI BssKI BstAPI BstF5I BstNI BstUI BstXI BstYI Bsu36I Cac8I CelII CfoI Cfr10I Cfr13I DdeI DpnII DraI DraII DraIII DsaI EaeI EagI EarI EclXI Eco52I Eco81I EcoNI EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeII HaeIII HapII HhaI Hin1I HinP1I HinfI HpaII HphI ItaI KasI Ksp632I KspI MaeI MaeII MaeIII MboI MboII MflI MnlI MseI MslI MspA1I MspI MvaI MvnI MwoI NaeI NarI NciI NdeII NgoMIV NlaIII NlaIV NspI PflMI PleI PpuMI PstI PvuII RsaI SacII SapI Sau3AI Sau96I ScaI ScrFI SfaNI SfcI SfoI SmaI SmlI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I Van91I XcmI XhoII XmaCI XmaI