##################################
# NO CUT ENZYMES ( 108 enzymes) #
##################################
AatII Acc65I AccII AccIII AclI
AcyI AflII AgeI AhdI Alw44I
Aor51HI ApaLI AscI Asp718I AspEI
AspI AviII BamHI BbeI BbrPI
BcgI BciVI BfrI BglI BlpI
Bpu1102I BsaAI BsaBI BsaHI BseAI
BsiEI BsiWI Bsp1407I BspDI BspEI
BspLU11I BsrFI BsrGI BssHII BstEII
BstPI BstUI CelII CfoI Cfr10I
ClaI CpoI DraIII DrdI EagI
Eam1105I EclXI Eco47III Eco52I EcoNI
EcoO65I EcoRV FseI FspI HaeII
HgaI HhaI Hin1I HinP1I HpaI
KasI KpnI KspI MamI MluI
MroI MvnI NaeI NarI NdeI
NgoMIV NheI NotI NruI PacI
PaeR7I PflFI PflMI PinAI PmaCI
PmeI PmlI Psp1406I PvuI RsrII
SacII SalI SapI SbfI ScaI
SexAI SfiI SfoI SgrAI SnaBI
SpeI Sse8387I SspBI SwaI Tth111I
Van91I XbaI XhoI
###################################
# ONE CUT ENZYMES ( 29 enzymes) #
###################################
AflIII 4790 ACGTGT A^C[GA][CT]GT
ApaI 197 GGGCCC GGGCC^C
AvrII 5197 CCTAGG C^CTAGG
BanI 804 GGCACC G^G[CT][GA]CC
BlnI 5197 CCTAGG C^CTAGG
BsaI 481 GGTCTC GGTCTC(1/5)
BsaWI 2447 ACCGGA [AT]^CCGG[AT]
BsmBI 3028 GAGACG CGTCTC(1/5)
BspHI 1594 TCATGA T^CATGA
BsrBI 2194 GAGCGG CCGCTC(-3/-3)
BssSI 2212 CTCGTG CACGAG(-5/-1)
Bst1107I 504 GTATAC GTA^TAC
BstAPI 1842 GCAAGACTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI 4483 TTCGAA TT^CGAA
BstZ17I 504 GTATAC GTA^TAC
EcoRI 4490 GAATTC G^AATTC
EcoT22I 4212 ATGCAT ATGCA^T
MfeI 4986 CAATTG C^AATTG
MunI 4986 CAATTG C^AATTG
NsiI 4212 ATGCAT ATGCA^T
NspV 4483 TTCGAA TT^CGAA
PpuMI 3217 AGGACCC [GA]G^G[AT]CC[CT]
PshAI 2701 GACTTGGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
RcaI 1594 TCATGA T^CATGA
SacI 1781 GAGCTC GAGCT^C
SfuI 4483 TTCGAA TT^CGAA
SphI 328 GCATGC GCATG^C
StuI 3952 AGGCCT AGG^CCT
XcmI 2131 CCATGGTTCTGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 25 enzymes) #
###################################
AspHI 1774 GAGCAC G[AT]GC[AT]^C
1781 GAGCTC
AvaI 222 CCCGGG C^[CT]CG[GA]G
4704 CCCGGG
BalI 1118 TGGCCA TGG^CCA
4060 TGGCCA
BanII 197 GGGCCC G[GA]GC[CT]^C
1781 GAGCTC
BbsI 299 GAAGAC GAAGAC(2/6)
518 GTCTTC
BbvCI 2958 CCTCAGC CCTCAGC(-5/-2)
3092 CCTCAGC
BclI 190 TGATCA T^GATCA
5049 TGATCA
BpmI 1410 CTCCAG CTGGAG(16/14)
4572 CTGGAG
BpuAI 299 GAAGAC GAAGAC(2/6)
518 GTCTTC
BsiHKAI 1774 GAGCAC G[AT]GC[AT]^C
1781 GAGCTC
BsoBI 222 CCCGGG C^[CT]CG[GA]G
4704 CCCGGG
BspMI 4126 ACCTGC ACCTGC(4/8)
3614 GCAGGT
BstXI 2968 CCAGGTAGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3102 CCAACGTGCTGG
DsaI 946 CCATGG C^C[GA][CT]GG
2131 CCATGG
EaeI 1118 TGGCCA [CT]^GGCC[GA]
4060 TGGCCA
FauI 3088 CCCGC CCCGC(4/6)
2196 GCGGG
FbaI 190 TGATCA T^GATCA
5049 TGATCA
HincII 4075 GTTGAC GT[CT]^[GA]AC
4452 GTTGAC
HindII 4075 GTTGAC GT[CT]^[GA]AC
4452 GTTGAC
MluNI 1118 TGGCCA TGG^CCA
4060 TGGCCA
MscI 1118 TGGCCA TGG^CCA
4060 TGGCCA
NcoI 946 CCATGG C^CATGG
2131 CCATGG
SmaI 222 CCCGGG CCC^GGG
4704 CCCGGG
XmaCI 222 CCCGGG C^CCGGG
4704 CCCGGG
XmaI 222 CCCGGG C^CCGGG
4704 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 96 enzymes) #
#########################################
AccI AciI AcsI AfaI AluI
AlwI AlwNI AocI ApoI AseI
AsnI Asp700I AvaII BbvI BcnI
BfaI BglII BmyI BsaJI BseRI
BsgI BsiYI BslI BsmAI BsmFI
BsmI Bsp1286I BsrDI BsrI BssKI
BstF5I BstNI BstYI Bsu36I Cac8I
Cfr13I DdeI DpnII DraI DraII
EarI Eco81I EcoO109I EcoRII EcoT14I
Fnu4HI FokI HaeIII HapII HindIII
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeII MaeIII MboI MboII
MflI MnlI MseI MslI MspA1I
MspI MvaI MwoI NciI NdeII
NlaIII NlaIV NspI PleI PshBI
PstI PvuII RsaI Sau3AI Sau96I
ScrFI SfaNI SfcI SmlI SspI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I XhoII
XmnI