##################################
# NO CUT ENZYMES ( 58 enzymes) #
##################################
AatII AclI AgeI AhdI AseI
AsnI AspEI AspI AviII AvrII
BbrPI BlnI BsaAI BsaBI BsiWI
BspDI BspHI ClaI CpoI DraIII
Eam1105I EcoRI EcoT22I FspI HpaI
MamI MfeI MluI MunI NdeI
NheI NotI NruI NsiI PacI
PflFI PinAI PmaCI PmeI PmlI
PshAI PshBI Psp1406I PvuI RcaI
RsrII SalI SbfI ScaI SexAI
SnaBI SpeI SphI Sse8387I SspI
SwaI Tth111I XbaI
###################################
# ONE CUT ENZYMES ( 49 enzymes) #
###################################
Acc65I 2518 GGTACC G^GTACC
AccI 3759 GTATAC GT^[AC][GT]AC
AcyI 2051 GGCGCC G[GA]^CG[CT]C
AflII 858 CTTAAG C^TTAAG
AflIII 3177 ACATGT A^C[GA][CT]GT
AocI 1710 CCTGAGG CC^T[ACGT]AGG
Aor51HI 1603 AGCGCT AGC^GCT
AscI 102 GGCGCGCC GG^CGCGCC
Asp718I 2518 GGTACC G^GTACC
BbeI 2051 GGCGCC GGCGC^C
BbvCI 771 GCTGAGG CCTCAGC(-5/-2)
BclI 4556 TGATCA T^GATCA
BfrI 858 CTTAAG C^TTAAG
BglII 4323 AGATCT A^GATCT
BsaHI 2051 GGCGCC G[GA]^CG[CT]C
Bsp1407I 4482 TGTACA T^GTACA
BspLU11I 3177 ACATGT A^CATGT
BsrGI 4482 TGTACA T^GTACA
Bst1107I 3759 GTATAC GTA^TAC
BstAPI 4622 GCATCTCTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI 462 TTCGAA TT^CGAA
BstEII 1279 GGTGACC G^GT[ACGT]ACC
BstPI 1279 GGTGACC G^GT[ACGT]ACC
BstZ17I 3759 GTATAC GTA^TAC
Bsu36I 1710 CCTGAGG CC^T[ACGT]AGG
DrdI 137 GACCCCGCGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 1603 AGCGCT AGC^GCT
Eco81I 1710 CCTGAGG CC^T[ACGT]AGG
EcoO65I 1279 GGTGACC G^GT[ACGT]ACC
EcoRV 4389 GATATC GAT^ATC
FbaI 4556 TGATCA T^GATCA
FseI 1568 GGCCGGCC GGCCGG^CC
Hin1I 2051 GGCGCC G[GA]^CG[CT]C
HincII 4470 GTTGAC GT[CT]^[GA]AC
HindII 4470 GTTGAC GT[CT]^[GA]AC
KasI 2051 GGCGCC G^GCGCC
KpnI 2518 GGTACC GGTAC^C
NarI 2051 GGCGCC GG^CGCC
NcoI 2318 CCATGG C^CATGG
NspV 462 TTCGAA TT^CGAA
PaeR7I 1312 CTCGAG C^TCGAG
SapI 475 GAAGAGC GCTCTTC(1/4)
SfiI 1144 GGCCAGGTGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfoI 2051 GGCGCC GGC^GCC
SfuI 462 TTCGAA TT^CGAA
SgrAI 29 CGCCGGCG C[GA]^CCGG[CT]G
SspBI 4482 TGTACA T^GTACA
StuI 824 AGGCCT AGG^CCT
XhoI 1312 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 16 enzymes) #
###################################
Alw44I 2701 GTGCAC G^TGCAC
3294 GTGCAC
ApaLI 2701 GTGCAC G^TGCAC
3294 GTGCAC
Asp700I 1458 GAACAAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
3332 GAACATCTTC
BamHI 1487 GGATCC G^GATCC
4651 GGATCC
BcgI 3684 CGATCCTCCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
3908 CGATTCGCCTGC
BsmI 894 GAATGC GAATGC(1/-1)
3039 GCATTC
BspMI 1214 ACCTGC ACCTGC(4/8)
2077 ACCTGC
BsrDI 2822 GCAATG GCAATG(2/0)
3859 GCAATG
BssHII 103 GCGCGC G^CGCGC
3949 GCGCGC
DraI 1637 TTTAAA TTT^AAA
3616 TTTAAA
EcoNI 335 CCTCCGTTAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
1710 CCTGAGGGAGG
HindIII 835 AAGCTT A^AGCTT
3288 AAGCTT
KspI 141 CCGCGG CCGC^GG
300 CCGCGG
MaeII 2446 ACGT A^CGT
3558 ACGT
SacII 141 CCGCGG CCGC^GG
300 CCGCGG
XmnI 1458 GAACAAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
3332 GAACATCTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 135 enzymes) #
#########################################
AccII AccIII AciI AcsI AfaI
AluI AlwI AlwNI ApaI ApoI
AspHI AvaI AvaII BalI BanI
BanII BbsI BbvI BciVI BcnI
BfaI BglI BlpI BmyI BpmI
Bpu1102I BpuAI BsaI BsaJI BsaWI
BseAI BseRI BsgI BsiEI BsiHKAI
BsiYI BslI BsmAI BsmBI BsmFI
BsoBI Bsp1286I BspEI BsrBI BsrFI
BsrI BssKI BssSI BstF5I BstNI
BstUI BstXI BstYI Cac8I CelII
CfoI Cfr10I Cfr13I DdeI DpnII
DraII DsaI EaeI EagI EarI
EclXI Eco52I EcoO109I EcoRII EcoT14I
FauI Fnu4HI FokI HaeII HaeIII
HapII HgaI HhaI HinP1I HinfI
HpaII HphI ItaI Ksp632I MaeI
MaeIII MboI MboII MflI MluNI
MnlI MroI MscI MseI MslI
MspA1I MspI MvaI MvnI MwoI
NaeI NciI NdeII NgoMIV NlaIII
NlaIV NspI PflMI PleI PpuMI
PstI PvuII RsaI SacI Sau3AI
Sau96I ScrFI SfaNI SfcI SmaI
SmlI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
Van91I XcmI XhoII XmaCI XmaI