##################################
# NO CUT ENZYMES ( 68 enzymes) #
##################################
AatII AccII Aor51HI AscI AviII
AvrII BbrPI BcgI BglI BlnI
BsaAI BsaBI BsiEI BsiWI BspDI
BspLU11I BssHII BssSI Bst1107I BstBI
BstUI BstZ17I ClaI CpoI EagI
EclXI Eco47III Eco52I EcoRV FseI
FspI HindIII KspI MamI MfeI
MluI MunI MvnI NaeI NgoMIV
NheI NotI NruI NspV PacI
PmaCI PmeI PmlI PshAI PvuI
RsrII SacII SalI SbfI ScaI
SfiI SfuI SgrAI SmaI SnaBI
SpeI SphI Sse8387I SspI StuI
SwaI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 46 enzymes) #
###################################
AccI 3656 GTCTAC GT^[AC][GT]AC
AccIII 3592 TCCGGA T^CCGGA
AclI 2097 AACGTT AA^CGTT
AcyI 3904 GGCGCC G[GA]^CG[CT]C
AflII 5120 CTTAAG C^TTAAG
AflIII 745 ACACGT A^C[GA][CT]GT
AgeI 97 ACCGGT A^CCGGT
AseI 2106 ATTAAT AT^TAAT
AsnI 2106 ATTAAT AT^TAAT
BalI 2937 TGGCCA TGG^CCA
BbeI 3904 GGCGCC GGCGC^C
BclI 5066 TGATCA T^GATCA
BfrI 5120 CTTAAG C^TTAAG
BsaHI 3904 GGCGCC G[GA]^CG[CT]C
BseAI 3592 TCCGGA T^CCGGA
BsgI 1114 CTGCAC GTGCAG(16/14)
BsmBI 841 GAGACG CGTCTC(1/5)
BspEI 3592 TCCGGA T^CCGGA
BspHI 1832 TCATGA T^CATGA
BspMI 1112 ACCTGC ACCTGC(4/8)
BsrDI 1844 CATTGC GCAATG(2/0)
BsrFI 97 ACCGGT [GA]^CCGG[CT]
BstXI 3255 CCAGCAGCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Cfr10I 97 ACCGGT [GA]^CCGG[CT]
DraIII 4274 CACCCCGTG CAC[ACGT][ACGT][ACGT]^GTG
EaeI 2937 TGGCCA [CT]^GGCC[GA]
EcoNI 3204 CCTCCCCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoT22I 3309 ATGCAT ATGCA^T
FbaI 5066 TGATCA T^GATCA
HaeII 3904 GGCGCC [GA]GCGC^[CT]
HgaI 1481 GACGC GACGC(5/10)
Hin1I 3904 GGCGCC G[GA]^CG[CT]C
KasI 3904 GGCGCC G^GCGCC
MluNI 2937 TGGCCA TGG^CCA
MroI 3592 TCCGGA T^CCGGA
MscI 2937 TGGCCA TGG^CCA
NarI 3904 GGCGCC GG^CGCC
NdeI 1254 CATATG CA^TATG
NsiI 3309 ATGCAT ATGCA^T
PinAI 97 ACCGGT A^CCGGT
PshBI 2106 ATTAAT AT^TAAT
Psp1406I 2097 AACGTT AA^CGTT
RcaI 1832 TCATGA T^CATGA
SfoI 3904 GGCGCC GGC^GCC
XbaI 4020 TCTAGA T^CTAGA
XcmI 378 CCAGGCGTGGAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 27 enzymes) #
###################################
Acc65I 1649 GGTACC G^GTACC
1670 GGTACC
AhdI 2858 GACCGCCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
4862 GACTGGATGTC
Alw44I 2675 GTGCAC G^TGCAC
5225 GTGCAC
ApaLI 2675 GTGCAC G^TGCAC
5225 GTGCAC
Asp718I 1649 GGTACC G^GTACC
1670 GGTACC
AspEI 2858 GACCGCCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
4862 GACTGGATGTC
BamHI 63 GGATCC G^GATCC
172 GGATCC
BlpI 3688 GCTGAGC GC^T[ACGT]AGC
4224 GCTCAGC
Bpu1102I 3688 GCTGAGC GC^T[ACGT]AGC
4224 GCTCAGC
Bsp1407I 1336 TGTACA T^GTACA
3108 TGTACA
BsrBI 1320 GAGCGG CCGCTC(-3/-3)
1638 GAGCGG
BsrGI 1336 TGTACA T^GTACA
3108 TGTACA
BstEII 1785 GGTCACC G^GT[ACGT]ACC
3020 GGTGACC
BstPI 1785 GGTCACC G^GT[ACGT]ACC
3020 GGTGACC
CelII 3688 GCTGAGC GC^T[ACGT]AGC
4224 GCTCAGC
CfoI 1848 GCGC GCG^C
3905 GCGC
DraI 3728 TTTAAA TTT^AAA
5179 TTTAAA
Eam1105I 2858 GACCGCCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
4862 GACTGGATGTC
EcoO65I 1785 GGTCACC G^GT[ACGT]ACC
3020 GGTGACC
HhaI 1848 GCGC GCG^C
3905 GCGC
HinP1I 1848 GCGC G^CGC
3905 GCGC
HpaI 2100 GTTAAC GTT^AAC
3770 GTTAAC
KpnI 1649 GGTACC GGTAC^C
1670 GGTACC
PaeR7I 1410 CTCGAG C^TCGAG
3722 CTCGAG
SacI 1345 GAGCTC GAGCT^C
4795 GAGCTC
SspBI 1336 TGTACA T^GTACA
3108 TGTACA
XhoI 1410 CTCGAG C^TCGAG
3722 CTCGAG
#########################################
# 3 OR MORE CUT ENZYMES ( 117 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
AlwNI AocI ApaI ApoI Asp700I
AspHI AspI AvaI AvaII BanI
BanII BbsI BbvCI BbvI BciVI
BcnI BfaI BglII BmyI BpmI
BpuAI BsaI BsaJI BsaWI BseRI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsmI BsoBI Bsp1286I BsrI BssKI
BstAPI BstF5I BstNI BstYI Bsu36I
Cac8I Cfr13I DdeI DpnII DraII
DrdI DsaI EarI Eco81I EcoO109I
EcoRI EcoRII EcoT14I FauI Fnu4HI
FokI HaeIII HapII HincII HindII
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeII MaeIII MboI MboII
MflI MnlI MseI MslI MspA1I
MspI MvaI MwoI NciI NcoI
NdeII NlaIII NlaIV NspI PflFI
PflMI PleI PpuMI PstI PvuII
RsaI SapI Sau3AI Sau96I ScrFI
SexAI SfaNI SfcI SmlI StyI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI Tth111I TthHB8I Van91I
XhoII XmnI