################################## # NO CUT ENZYMES ( 68 enzymes) # ################################## AatII AccII Aor51HI AscI AviII AvrII BbrPI BcgI BglI BlnI BsaAI BsaBI BsiEI BsiWI BspDI BspLU11I BssHII BssSI Bst1107I BstBI BstUI BstZ17I ClaI CpoI EagI EclXI Eco47III Eco52I EcoRV FseI FspI HindIII KspI MamI MfeI MluI MunI MvnI NaeI NgoMIV NheI NotI NruI NspV PacI PmaCI PmeI PmlI PshAI PvuI RsrII SacII SalI SbfI ScaI SfiI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspI StuI SwaI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 46 enzymes) # ################################### AccI 3656 GTCTAC GT^[AC][GT]AC AccIII 3592 TCCGGA T^CCGGA AclI 2097 AACGTT AA^CGTT AcyI 3904 GGCGCC G[GA]^CG[CT]C AflII 5120 CTTAAG C^TTAAG AflIII 745 ACACGT A^C[GA][CT]GT AgeI 97 ACCGGT A^CCGGT AseI 2106 ATTAAT AT^TAAT AsnI 2106 ATTAAT AT^TAAT BalI 2937 TGGCCA TGG^CCA BbeI 3904 GGCGCC GGCGC^C BclI 5066 TGATCA T^GATCA BfrI 5120 CTTAAG C^TTAAG BsaHI 3904 GGCGCC G[GA]^CG[CT]C BseAI 3592 TCCGGA T^CCGGA BsgI 1114 CTGCAC GTGCAG(16/14) BsmBI 841 GAGACG CGTCTC(1/5) BspEI 3592 TCCGGA T^CCGGA BspHI 1832 TCATGA T^CATGA BspMI 1112 ACCTGC ACCTGC(4/8) BsrDI 1844 CATTGC GCAATG(2/0) BsrFI 97 ACCGGT [GA]^CCGG[CT] BstXI 3255 CCAGCAGCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG Cfr10I 97 ACCGGT [GA]^CCGG[CT] DraIII 4274 CACCCCGTG CAC[ACGT][ACGT][ACGT]^GTG EaeI 2937 TGGCCA [CT]^GGCC[GA] EcoNI 3204 CCTCCCCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoT22I 3309 ATGCAT ATGCA^T FbaI 5066 TGATCA T^GATCA HaeII 3904 GGCGCC [GA]GCGC^[CT] HgaI 1481 GACGC GACGC(5/10) Hin1I 3904 GGCGCC G[GA]^CG[CT]C KasI 3904 GGCGCC G^GCGCC MluNI 2937 TGGCCA TGG^CCA MroI 3592 TCCGGA T^CCGGA MscI 2937 TGGCCA TGG^CCA NarI 3904 GGCGCC GG^CGCC NdeI 1254 CATATG CA^TATG NsiI 3309 ATGCAT ATGCA^T PinAI 97 ACCGGT A^CCGGT PshBI 2106 ATTAAT AT^TAAT Psp1406I 2097 AACGTT AA^CGTT RcaI 1832 TCATGA T^CATGA SfoI 3904 GGCGCC GGC^GCC XbaI 4020 TCTAGA T^CTAGA XcmI 378 CCAGGCGTGGAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG ################################### # TWO CUT ENZYMES ( 27 enzymes) # ################################### Acc65I 1649 GGTACC G^GTACC 1670 GGTACC AhdI 2858 GACCGCCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 4862 GACTGGATGTC Alw44I 2675 GTGCAC G^TGCAC 5225 GTGCAC ApaLI 2675 GTGCAC G^TGCAC 5225 GTGCAC Asp718I 1649 GGTACC G^GTACC 1670 GGTACC AspEI 2858 GACCGCCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 4862 GACTGGATGTC BamHI 63 GGATCC G^GATCC 172 GGATCC BlpI 3688 GCTGAGC GC^T[ACGT]AGC 4224 GCTCAGC Bpu1102I 3688 GCTGAGC GC^T[ACGT]AGC 4224 GCTCAGC Bsp1407I 1336 TGTACA T^GTACA 3108 TGTACA BsrBI 1320 GAGCGG CCGCTC(-3/-3) 1638 GAGCGG BsrGI 1336 TGTACA T^GTACA 3108 TGTACA BstEII 1785 GGTCACC G^GT[ACGT]ACC 3020 GGTGACC BstPI 1785 GGTCACC G^GT[ACGT]ACC 3020 GGTGACC CelII 3688 GCTGAGC GC^T[ACGT]AGC 4224 GCTCAGC CfoI 1848 GCGC GCG^C 3905 GCGC DraI 3728 TTTAAA TTT^AAA 5179 TTTAAA Eam1105I 2858 GACCGCCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 4862 GACTGGATGTC EcoO65I 1785 GGTCACC G^GT[ACGT]ACC 3020 GGTGACC HhaI 1848 GCGC GCG^C 3905 GCGC HinP1I 1848 GCGC G^CGC 3905 GCGC HpaI 2100 GTTAAC GTT^AAC 3770 GTTAAC KpnI 1649 GGTACC GGTAC^C 1670 GGTACC PaeR7I 1410 CTCGAG C^TCGAG 3722 CTCGAG SacI 1345 GAGCTC GAGCT^C 4795 GAGCTC SspBI 1336 TGTACA T^GTACA 3108 TGTACA XhoI 1410 CTCGAG C^TCGAG 3722 CTCGAG ######################################### # 3 OR MORE CUT ENZYMES ( 117 enzymes) # ######################################### AciI AcsI AfaI AluI AlwI AlwNI AocI ApaI ApoI Asp700I AspHI AspI AvaI AvaII BanI BanII BbsI BbvCI BbvI BciVI BcnI BfaI BglII BmyI BpmI BpuAI BsaI BsaJI BsaWI BseRI BsiHKAI BsiYI BslI BsmAI BsmFI BsmI BsoBI Bsp1286I BsrI BssKI BstAPI BstF5I BstNI BstYI Bsu36I Cac8I Cfr13I DdeI DpnII DraII DrdI DsaI EarI Eco81I EcoO109I EcoRI EcoRII EcoT14I FauI Fnu4HI FokI HaeIII HapII HincII HindII HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MnlI MseI MslI MspA1I MspI MvaI MwoI NciI NcoI NdeII NlaIII NlaIV NspI PflFI PflMI PleI PpuMI PstI PvuII RsaI SapI Sau3AI Sau96I ScrFI SexAI SfaNI SfcI SmlI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI Tth111I TthHB8I Van91I XhoII XmnI