##################################
# NO CUT ENZYMES ( 72 enzymes) #
##################################
Acc65I AccI AccIII AclI AhdI
Alw44I Aor51HI ApaLI AscI AseI
AsnI Asp718I AspEI AviII BamHI
BbrPI BcgI BsaBI BseAI BsiWI
BspDI BspEI BssHII Bst1107I BstBI
BstZ17I ClaI CpoI DraIII EagI
Eam1105I EclXI Eco47III Eco52I FseI
FspI HpaI KpnI KspI MamI
MluI MroI NotI NruI NspV
PacI PaeR7I PmaCI PmeI PmlI
PshAI PshBI Psp1406I PvuI RsrII
SacII SalI SbfI ScaI SexAI
SfiI SfuI SgrAI SmaI SnaBI
SpeI SphI Sse8387I SwaI XhoI
XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 32 enzymes) #
###################################
AlwNI 838 CAGATCCTG CAG[ACGT][ACGT][ACGT]^CTG
ApaI 1747 GGGCCC GGGCC^C
AvrII 4745 CCTAGG C^CTAGG
BbvCI 2031 GCTGAGG CCTCAGC(-5/-2)
BciVI 1404 GGATAC GTATCC(6/5)
BclI 3635 TGATCA T^GATCA
BglII 4327 AGATCT A^GATCT
BlnI 4745 CCTAGG C^CTAGG
BpmI 2048 CTCCAG CTGGAG(16/14)
BsaAI 4631 TACGTG [CT]AC^GT[GA]
BsiEI 2074 CGGTCG CG[GA][CT]^CG
BsmBI 1078 GAGACG CGTCTC(1/5)
BsrBI 615 GAGCGG CCGCTC(-3/-3)
BssSI 1999 CACGAG CACGAG(-5/-1)
BstEII 418 GGTGACC G^GT[ACGT]ACC
BstPI 418 GGTGACC G^GT[ACGT]ACC
EcoO65I 418 GGTGACC G^GT[ACGT]ACC
EcoRI 4455 GAATTC G^AATTC
EcoT22I 3127 ATGCAT ATGCA^T
FbaI 3635 TGATCA T^GATCA
MfeI 4480 CAATTG C^AATTG
MunI 4480 CAATTG C^AATTG
NaeI 654 GCCGGC GCC^GGC
NgoMIV 654 GCCGGC G^CCGGC
NheI 4492 GCTAGC G^CTAGC
NsiI 3127 ATGCAT ATGCA^T
PflMI 1667 CCAAGATATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
StuI 903 AGGCCT AGG^CCT
TaqI 809 TCGA T^CGA
TthHB8I 809 TCGA T^CGA
Van91I 1667 CCAAGATATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 786 CCACATAGCCTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 32 enzymes) #
###################################
AatII 817 GACGTC GACGT^C
1324 GACGTC
AccII 1082 CGCG CG^CG
1158 CGCG
AgeI 319 ACCGGT A^CCGGT
415 ACCGGT
AocI 1399 CCTCAGG CC^T[ACGT]AGG
4791 CCTTAGG
AspI 567 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
682 GACAAGGTC
AvaI 534 CTCGGG C^[CT]CG[GA]G
1750 CCCGAG
BbeI 77 GGCGCC GGCGC^C
1886 GGCGCC
BglI 1816 GCCGTCGGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
1852 GCCTTTGCGGC
BsaI 3148 GAGACC GGTCTC(1/5)
589 GGTCTC
BsaWI 319 ACCGGT [AT]^CCGG[AT]
415 ACCGGT
BseRI 1831 CTCCTC GAGGAG(10/8)
481 GAGGAG
BsgI 1153 CTGCAC GTGCAG(16/14)
2758 GTGCAG
BsmI 29 GCATTC GAATGC(1/-1)
3138 GCATTC
BsoBI 534 CTCGGG C^[CT]CG[GA]G
1750 CCCGAG
BspHI 255 TCATGA T^CATGA
3191 TCATGA
BstUI 1082 CGCG CG^CG
1158 CGCG
Bsu36I 1399 CCTCAGG CC^T[ACGT]AGG
4791 CCTTAGG
Eco81I 1399 CCTCAGG CC^T[ACGT]AGG
4791 CCTTAGG
EcoNI 3356 CCTATTTCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
4740 CCTTTCCTAGG
HincII 564 GTTGAC GT[CT]^[GA]AC
889 GTCAAC
HindII 564 GTTGAC GT[CT]^[GA]AC
889 GTCAAC
HindIII 2892 AAGCTT A^AGCTT
3386 AAGCTT
KasI 77 GGCGCC G^GCGCC
1886 GGCGCC
MvnI 1082 CGCG CG^CG
1158 CGCG
NarI 77 GGCGCC GG^CGCC
1886 GGCGCC
PflFI 567 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
682 GACAAGGTC
PinAI 319 ACCGGT A^CCGGT
415 ACCGGT
RcaI 255 TCATGA T^CATGA
3191 TCATGA
SapI 1296 GCTCTTC GCTCTTC(1/4)
2650 GCTCTTC
SfoI 77 GGCGCC GGC^GCC
1886 GGCGCC
Tth111I 567 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
682 GACAAGGTC
XbaI 3982 TCTAGA T^CTAGA
4128 TCTAGA
#########################################
# 3 OR MORE CUT ENZYMES ( 122 enzymes) #
#########################################
AciI AcsI AcyI AfaI AflII
AflIII AluI AlwI ApoI Asp700I
AspHI AvaII BalI BanI BanII
BbsI BbvI BcnI BfaI BfrI
BlpI BmyI Bpu1102I BpuAI BsaHI
BsaJI BsiHKAI BsiYI BslI BsmAI
BsmFI Bsp1286I Bsp1407I BspLU11I BspMI
BsrDI BsrFI BsrGI BsrI BssKI
BstAPI BstF5I BstNI BstXI BstYI
Cac8I CelII CfoI Cfr10I Cfr13I
DdeI DpnII DraI DraII DrdI
DsaI EaeI EarI EcoO109I EcoRII
EcoRV EcoT14I FauI Fnu4HI FokI
HaeII HaeIII HapII HgaI HhaI
Hin1I HinP1I HinfI HpaII HphI
ItaI Ksp632I MaeI MaeII MaeIII
MboI MboII MflI MluNI MnlI
MscI MseI MslI MspA1I MspI
MvaI MwoI NciI NcoI NdeI
NdeII NlaIII NlaIV NspI PleI
PpuMI PstI PvuII RsaI SacI
Sau3AI Sau96I ScrFI SfaNI SfcI
SmlI SspBI SspI StyI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
XhoII XmnI