################################## # NO CUT ENZYMES ( 72 enzymes) # ################################## Acc65I AccI AccIII AclI AhdI Alw44I Aor51HI ApaLI AscI AseI AsnI Asp718I AspEI AviII BamHI BbrPI BcgI BsaBI BseAI BsiWI BspDI BspEI BssHII Bst1107I BstBI BstZ17I ClaI CpoI DraIII EagI Eam1105I EclXI Eco47III Eco52I FseI FspI HpaI KpnI KspI MamI MluI MroI NotI NruI NspV PacI PaeR7I PmaCI PmeI PmlI PshAI PshBI Psp1406I PvuI RsrII SacII SalI SbfI ScaI SexAI SfiI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I SwaI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 32 enzymes) # ################################### AlwNI 838 CAGATCCTG CAG[ACGT][ACGT][ACGT]^CTG ApaI 1747 GGGCCC GGGCC^C AvrII 4745 CCTAGG C^CTAGG BbvCI 2031 GCTGAGG CCTCAGC(-5/-2) BciVI 1404 GGATAC GTATCC(6/5) BclI 3635 TGATCA T^GATCA BglII 4327 AGATCT A^GATCT BlnI 4745 CCTAGG C^CTAGG BpmI 2048 CTCCAG CTGGAG(16/14) BsaAI 4631 TACGTG [CT]AC^GT[GA] BsiEI 2074 CGGTCG CG[GA][CT]^CG BsmBI 1078 GAGACG CGTCTC(1/5) BsrBI 615 GAGCGG CCGCTC(-3/-3) BssSI 1999 CACGAG CACGAG(-5/-1) BstEII 418 GGTGACC G^GT[ACGT]ACC BstPI 418 GGTGACC G^GT[ACGT]ACC EcoO65I 418 GGTGACC G^GT[ACGT]ACC EcoRI 4455 GAATTC G^AATTC EcoT22I 3127 ATGCAT ATGCA^T FbaI 3635 TGATCA T^GATCA MfeI 4480 CAATTG C^AATTG MunI 4480 CAATTG C^AATTG NaeI 654 GCCGGC GCC^GGC NgoMIV 654 GCCGGC G^CCGGC NheI 4492 GCTAGC G^CTAGC NsiI 3127 ATGCAT ATGCA^T PflMI 1667 CCAAGATATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG StuI 903 AGGCCT AGG^CCT TaqI 809 TCGA T^CGA TthHB8I 809 TCGA T^CGA Van91I 1667 CCAAGATATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XcmI 786 CCACATAGCCTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG ################################### # TWO CUT ENZYMES ( 32 enzymes) # ################################### AatII 817 GACGTC GACGT^C 1324 GACGTC AccII 1082 CGCG CG^CG 1158 CGCG AgeI 319 ACCGGT A^CCGGT 415 ACCGGT AocI 1399 CCTCAGG CC^T[ACGT]AGG 4791 CCTTAGG AspI 567 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC 682 GACAAGGTC AvaI 534 CTCGGG C^[CT]CG[GA]G 1750 CCCGAG BbeI 77 GGCGCC GGCGC^C 1886 GGCGCC BglI 1816 GCCGTCGGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC 1852 GCCTTTGCGGC BsaI 3148 GAGACC GGTCTC(1/5) 589 GGTCTC BsaWI 319 ACCGGT [AT]^CCGG[AT] 415 ACCGGT BseRI 1831 CTCCTC GAGGAG(10/8) 481 GAGGAG BsgI 1153 CTGCAC GTGCAG(16/14) 2758 GTGCAG BsmI 29 GCATTC GAATGC(1/-1) 3138 GCATTC BsoBI 534 CTCGGG C^[CT]CG[GA]G 1750 CCCGAG BspHI 255 TCATGA T^CATGA 3191 TCATGA BstUI 1082 CGCG CG^CG 1158 CGCG Bsu36I 1399 CCTCAGG CC^T[ACGT]AGG 4791 CCTTAGG Eco81I 1399 CCTCAGG CC^T[ACGT]AGG 4791 CCTTAGG EcoNI 3356 CCTATTTCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG 4740 CCTTTCCTAGG HincII 564 GTTGAC GT[CT]^[GA]AC 889 GTCAAC HindII 564 GTTGAC GT[CT]^[GA]AC 889 GTCAAC HindIII 2892 AAGCTT A^AGCTT 3386 AAGCTT KasI 77 GGCGCC G^GCGCC 1886 GGCGCC MvnI 1082 CGCG CG^CG 1158 CGCG NarI 77 GGCGCC GG^CGCC 1886 GGCGCC PflFI 567 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC 682 GACAAGGTC PinAI 319 ACCGGT A^CCGGT 415 ACCGGT RcaI 255 TCATGA T^CATGA 3191 TCATGA SapI 1296 GCTCTTC GCTCTTC(1/4) 2650 GCTCTTC SfoI 77 GGCGCC GGC^GCC 1886 GGCGCC Tth111I 567 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC 682 GACAAGGTC XbaI 3982 TCTAGA T^CTAGA 4128 TCTAGA ######################################### # 3 OR MORE CUT ENZYMES ( 122 enzymes) # ######################################### AciI AcsI AcyI AfaI AflII AflIII AluI AlwI ApoI Asp700I AspHI AvaII BalI BanI BanII BbsI BbvI BcnI BfaI BfrI BlpI BmyI Bpu1102I BpuAI BsaHI BsaJI BsiHKAI BsiYI BslI BsmAI BsmFI Bsp1286I Bsp1407I BspLU11I BspMI BsrDI BsrFI BsrGI BsrI BssKI BstAPI BstF5I BstNI BstXI BstYI Cac8I CelII CfoI Cfr10I Cfr13I DdeI DpnII DraI DraII DrdI DsaI EaeI EarI EcoO109I EcoRII EcoRV EcoT14I FauI Fnu4HI FokI HaeII HaeIII HapII HgaI HhaI Hin1I HinP1I HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MluNI MnlI MscI MseI MslI MspA1I MspI MvaI MwoI NciI NcoI NdeI NdeII NlaIII NlaIV NspI PleI PpuMI PstI PvuII RsaI SacI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI SspBI SspI StyI TfiI Tru9I TseI Tsp45I Tsp509I TspRI XhoII XmnI