##################################
# NO CUT ENZYMES (   72 enzymes) #
##################################

Acc65I      AccI        AccIII      AclI        AhdI        
Alw44I      Aor51HI     ApaLI       AscI        AseI        
AsnI        Asp718I     AspEI       AviII       BamHI       
BbrPI       BcgI        BsaBI       BseAI       BsiWI       
BspDI       BspEI       BssHII      Bst1107I    BstBI       
BstZ17I     ClaI        CpoI        DraIII      EagI        
Eam1105I    EclXI       Eco47III    Eco52I      FseI        
FspI        HpaI        KpnI        KspI        MamI        
MluI        MroI        NotI        NruI        NspV        
PacI        PaeR7I      PmaCI       PmeI        PmlI        
PshAI       PshBI       Psp1406I    PvuI        RsrII       
SacII       SalI        SbfI        ScaI        SexAI       
SfiI        SfuI        SgrAI       SmaI        SnaBI       
SpeI        SphI        Sse8387I    SwaI        XhoI        
XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   32 enzymes) #
###################################

AlwNI         838  CAGATCCTG            CAG[ACGT][ACGT][ACGT]^CTG
ApaI         1747  GGGCCC               GGGCC^C
AvrII        4745  CCTAGG               C^CTAGG
BbvCI        2031  GCTGAGG              CCTCAGC(-5/-2)
BciVI        1404  GGATAC               GTATCC(6/5)
BclI         3635  TGATCA               T^GATCA
BglII        4327  AGATCT               A^GATCT
BlnI         4745  CCTAGG               C^CTAGG
BpmI         2048  CTCCAG               CTGGAG(16/14)
BsaAI        4631  TACGTG               [CT]AC^GT[GA]
BsiEI        2074  CGGTCG               CG[GA][CT]^CG
BsmBI        1078  GAGACG               CGTCTC(1/5)
BsrBI         615  GAGCGG               CCGCTC(-3/-3)
BssSI        1999  CACGAG               CACGAG(-5/-1)
BstEII        418  GGTGACC              G^GT[ACGT]ACC
BstPI         418  GGTGACC              G^GT[ACGT]ACC
EcoO65I       418  GGTGACC              G^GT[ACGT]ACC
EcoRI        4455  GAATTC               G^AATTC
EcoT22I      3127  ATGCAT               ATGCA^T
FbaI         3635  TGATCA               T^GATCA
MfeI         4480  CAATTG               C^AATTG
MunI         4480  CAATTG               C^AATTG
NaeI          654  GCCGGC               GCC^GGC
NgoMIV        654  GCCGGC               G^CCGGC
NheI         4492  GCTAGC               G^CTAGC
NsiI         3127  ATGCAT               ATGCA^T
PflMI        1667  CCAAGATATGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
StuI          903  AGGCCT               AGG^CCT
TaqI          809  TCGA                 T^CGA
TthHB8I       809  TCGA                 T^CGA
Van91I       1667  CCAAGATATGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI          786  CCACATAGCCTTTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG


###################################
# TWO CUT ENZYMES (   32 enzymes) #
###################################

AatII         817  GACGTC               GACGT^C
             1324  GACGTC              
AccII        1082  CGCG                 CG^CG
             1158  CGCG                
AgeI          319  ACCGGT               A^CCGGT
              415  ACCGGT              
AocI         1399  CCTCAGG              CC^T[ACGT]AGG
             4791  CCTTAGG             
AspI          567  GACCCTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
              682  GACAAGGTC           
AvaI          534  CTCGGG               C^[CT]CG[GA]G
             1750  CCCGAG              
BbeI           77  GGCGCC               GGCGC^C
             1886  GGCGCC              
BglI         1816  GCCGTCGGGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
             1852  GCCTTTGCGGC         
BsaI         3148  GAGACC               GGTCTC(1/5)
              589  GGTCTC              
BsaWI         319  ACCGGT               [AT]^CCGG[AT]
              415  ACCGGT              
BseRI        1831  CTCCTC               GAGGAG(10/8)
              481  GAGGAG              
BsgI         1153  CTGCAC               GTGCAG(16/14)
             2758  GTGCAG              
BsmI           29  GCATTC               GAATGC(1/-1)
             3138  GCATTC              
BsoBI         534  CTCGGG               C^[CT]CG[GA]G
             1750  CCCGAG              
BspHI         255  TCATGA               T^CATGA
             3191  TCATGA              
BstUI        1082  CGCG                 CG^CG
             1158  CGCG                
Bsu36I       1399  CCTCAGG              CC^T[ACGT]AGG
             4791  CCTTAGG             
Eco81I       1399  CCTCAGG              CC^T[ACGT]AGG
             4791  CCTTAGG             
EcoNI        3356  CCTATTTCAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
             4740  CCTTTCCTAGG         
HincII        564  GTTGAC               GT[CT]^[GA]AC
              889  GTCAAC              
HindII        564  GTTGAC               GT[CT]^[GA]AC
              889  GTCAAC              
HindIII      2892  AAGCTT               A^AGCTT
             3386  AAGCTT              
KasI           77  GGCGCC               G^GCGCC
             1886  GGCGCC              
MvnI         1082  CGCG                 CG^CG
             1158  CGCG                
NarI           77  GGCGCC               GG^CGCC
             1886  GGCGCC              
PflFI         567  GACCCTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
              682  GACAAGGTC           
PinAI         319  ACCGGT               A^CCGGT
              415  ACCGGT              
RcaI          255  TCATGA               T^CATGA
             3191  TCATGA              
SapI         1296  GCTCTTC              GCTCTTC(1/4)
             2650  GCTCTTC             
SfoI           77  GGCGCC               GGC^GCC
             1886  GGCGCC              
Tth111I       567  GACCCTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
              682  GACAAGGTC           
XbaI         3982  TCTAGA               T^CTAGA
             4128  TCTAGA              


#########################################
# 3 OR MORE CUT ENZYMES (  122 enzymes) #
#########################################

AciI        AcsI        AcyI        AfaI        AflII       
AflIII      AluI        AlwI        ApoI        Asp700I     
AspHI       AvaII       BalI        BanI        BanII       
BbsI        BbvI        BcnI        BfaI        BfrI        
BlpI        BmyI        Bpu1102I    BpuAI       BsaHI       
BsaJI       BsiHKAI     BsiYI       BslI        BsmAI       
BsmFI       Bsp1286I    Bsp1407I    BspLU11I    BspMI       
BsrDI       BsrFI       BsrGI       BsrI        BssKI       
BstAPI      BstF5I      BstNI       BstXI       BstYI       
Cac8I       CelII       CfoI        Cfr10I      Cfr13I      
DdeI        DpnII       DraI        DraII       DrdI        
DsaI        EaeI        EarI        EcoO109I    EcoRII      
EcoRV       EcoT14I     FauI        Fnu4HI      FokI        
HaeII       HaeIII      HapII       HgaI        HhaI        
Hin1I       HinP1I      HinfI       HpaII       HphI        
ItaI        Ksp632I     MaeI        MaeII       MaeIII      
MboI        MboII       MflI        MluNI       MnlI        
MscI        MseI        MslI        MspA1I      MspI        
MvaI        MwoI        NciI        NcoI        NdeI        
NdeII       NlaIII      NlaIV       NspI        PleI        
PpuMI       PstI        PvuII       RsaI        SacI        
Sau3AI      Sau96I      ScrFI       SfaNI       SfcI        
SmlI        SspBI       SspI        StyI        TfiI        
Tru9I       TseI        Tsp45I      Tsp509I     TspRI       
XhoII       XmnI