##################################
# NO CUT ENZYMES (   82 enzymes) #
##################################

AatII       AccIII      AclI        AcyI        AhdI        
Aor51HI     AscI        AspEI       AvaI        AviII       
BalI        BbeI        BbrPI       BcgI        BsaAI       
BsaBI       BsaHI       BseAI       BsiEI       BsiWI       
BsmBI       BsmI        BsoBI       BspDI       BspEI       
BsrBI       BssHII      BssSI       BstBI       ClaI        
CpoI        DraIII      DrdI        EaeI        EagI        
Eam1105I    EclXI       Eco47III    Eco52I      EcoNI       
EcoRV       FseI        FspI        HaeII       HgaI        
Hin1I       KasI        KspI        MamI        MluI        
MluNI       MroI        MscI        NarI        NheI        
NotI        NruI        NspV        PacI        PaeR7I      
PmaCI       PmeI        PmlI        PpuMI       Psp1406I    
PvuI        RsrII       SacII       SalI        SbfI        
SfiI        SfoI        SfuI        SgrAI       SmaI        
SnaBI       Sse8387I    SspI        SwaI        XhoI        
XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   43 enzymes) #
###################################

Acc65I        158  GGTACC               G^GTACC
AccI          848  GTATAC               GT^[AC][GT]AC
AccII          82  CGCG                 CG^CG
AflIII       4228  ACATGT               A^C[GA][CT]GT
AgeI          283  ACCGGT               A^CCGGT
Asp718I       158  GGTACC               G^GTACC
AspI          884  GACACTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
AvrII        4487  CCTAGG               C^CTAGG
BbvCI        1073  CCTCAGC              CCTCAGC(-5/-2)
BciVI         973  GTATCC               GTATCC(6/5)
BglI          663  GCCTGGGAGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlnI         4487  CCTAGG               C^CTAGG
BsaWI         283  ACCGGT               [AT]^CCGG[AT]
Bsp1407I     4481  TGTACA               T^GTACA
BspHI        1641  TCATGA               T^CATGA
BspLU11I     4228  ACATGT               A^CATGT
BsrGI        4481  TGTACA               T^GTACA
Bst1107I      848  GTATAC               GTA^TAC
BstEII        770  GGTGACC              G^GT[ACGT]ACC
BstPI         770  GGTGACC              G^GT[ACGT]ACC
BstUI          82  CGCG                 CG^CG
BstZ17I       848  GTATAC               GTA^TAC
DraI         3838  TTTAAA               TTT^AAA
EcoO65I       770  GGTGACC              G^GT[ACGT]ACC
EcoRI        2103  GAATTC               G^AATTC
HincII       3662  GTTAAC               GT[CT]^[GA]AC
HindII       3662  GTTAAC               GT[CT]^[GA]AC
HpaI         3662  GTTAAC               GTT^AAC
KpnI          158  GGTACC               GGTAC^C
MfeI         3332  CAATTG               C^AATTG
MunI         3332  CAATTG               C^AATTG
MvnI           82  CGCG                 CG^CG
NaeI          458  GCCGGC               GCC^GGC
NdeI         4120  CATATG               CA^TATG
NgoMIV        458  GCCGGC               G^CCGGC
PflFI         884  GACACTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
PinAI         283  ACCGGT               A^CCGGT
RcaI         1641  TCATGA               T^CATGA
SpeI         3984  ACTAGT               A^CTAGT
SspBI        4481  TGTACA               T^GTACA
Tth111I       884  GACACTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
XbaI         2981  TCTAGA               T^CTAGA
XcmI         1468  CCAAACAGGGCGTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG


###################################
# TWO CUT ENZYMES (   26 enzymes) #
###################################

Alw44I        906  GTGCAC               G^TGCAC
             3313  GTGCAC              
ApaI         2238  GGGCCC               GGGCC^C
             4324  GGGCCC              
ApaLI         906  GTGCAC               G^TGCAC
             3313  GTGCAC              
AseI         1509  ATTAAT               AT^TAAT
             3280  ATTAAT              
AsnI         1509  ATTAAT               AT^TAAT
             3280  ATTAAT              
BamHI        1265  GGATCC               G^GATCC
             4381  GGATCC              
BclI          691  TGATCA               T^GATCA
             4534  TGATCA              
BcnI          155  CCCGG                CC^[GC]GG
             1034  CCGGG               
BglII         340  AGATCT               A^GATCT
             3833  AGATCT              
BlpI          110  GCTGAGC              GC^T[ACGT]AGC
              554  GCTGAGC             
Bpu1102I      110  GCTGAGC              GC^T[ACGT]AGC
              554  GCTGAGC             
BsrDI         436  CATTGC               GCAATG(2/0)
             1983  GCAATG              
BstAPI         98  GCACTCCCTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
              449  GCAGAAGCTGC         
CelII         110  GCTGAGC              GC^T[ACGT]AGC
              554  GCTGAGC             
EcoT22I      3210  ATGCAT               ATGCA^T
             3226  ATGCAT              
FbaI          691  TGATCA               T^GATCA
             4534  TGATCA              
HindIII      3059  AAGCTT               A^AGCTT
             3417  AAGCTT              
MaeII        2227  ACGT                 A^CGT
             2948  ACGT                
NciI          155  CCCGG                CC^[GC]GG
             1034  CCGGG               
NsiI         3210  ATGCAT               ATGCA^T
             3226  ATGCAT              
PflMI         266  CCACATCATGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
              727  CCAAGGTGTGG         
PshAI          33  GACCTCAGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
             3125  GACTGCTGTC          
PshBI        1509  ATTAAT               AT^TAAT
             3280  ATTAAT              
ScaI          236  AGTACT               AGT^ACT
             2661  AGTACT              
SexAI        3520  ACCTGGT              A^CC[AT]GGT
             4009  ACCTGGT             
Van91I        266  CCACATCATGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
              727  CCAAGGTGTGG         


#########################################
# 3 OR MORE CUT ENZYMES (  107 enzymes) #
#########################################

AciI        AcsI        AfaI        AflII       AluI        
AlwI        AlwNI       AocI        ApoI        Asp700I     
AspHI       AvaII       BanI        BanII       BbsI        
BbvI        BfaI        BfrI        BmyI        BpmI        
BpuAI       BsaI        BsaJI       BseRI       BsgI        
BsiHKAI     BsiYI       BslI        BsmAI       BsmFI       
Bsp1286I    BspMI       BsrFI       BsrI        BssKI       
BstF5I      BstNI       BstXI       BstYI       Bsu36I      
Cac8I       CfoI        Cfr10I      Cfr13I      DdeI        
DpnII       DraII       DsaI        EarI        Eco81I      
EcoO109I    EcoRII      EcoT14I     FauI        Fnu4HI      
FokI        HaeIII      HapII       HhaI        HinP1I      
HinfI       HpaII       HphI        ItaI        Ksp632I     
MaeI        MaeIII      MboI        MboII       MflI        
MnlI        MseI        MslI        MspA1I      MspI        
MvaI        MwoI        NcoI        NdeII       NlaIII      
NlaIV       NspI        PleI        PstI        PvuII       
RsaI        SacI        SapI        Sau3AI      Sau96I      
ScrFI       SfaNI       SfcI        SmlI        SphI        
StuI        StyI        TaqI        TfiI        Tru9I       
TseI        Tsp45I      Tsp509I     TspRI       TthHB8I     
XhoII       XmnI