##################################
# NO CUT ENZYMES ( 82 enzymes) #
##################################
AatII AccIII AclI AcyI AhdI
Aor51HI AscI AspEI AvaI AviII
BalI BbeI BbrPI BcgI BsaAI
BsaBI BsaHI BseAI BsiEI BsiWI
BsmBI BsmI BsoBI BspDI BspEI
BsrBI BssHII BssSI BstBI ClaI
CpoI DraIII DrdI EaeI EagI
Eam1105I EclXI Eco47III Eco52I EcoNI
EcoRV FseI FspI HaeII HgaI
Hin1I KasI KspI MamI MluI
MluNI MroI MscI NarI NheI
NotI NruI NspV PacI PaeR7I
PmaCI PmeI PmlI PpuMI Psp1406I
PvuI RsrII SacII SalI SbfI
SfiI SfoI SfuI SgrAI SmaI
SnaBI Sse8387I SspI SwaI XhoI
XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 43 enzymes) #
###################################
Acc65I 158 GGTACC G^GTACC
AccI 848 GTATAC GT^[AC][GT]AC
AccII 82 CGCG CG^CG
AflIII 4228 ACATGT A^C[GA][CT]GT
AgeI 283 ACCGGT A^CCGGT
Asp718I 158 GGTACC G^GTACC
AspI 884 GACACTGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvrII 4487 CCTAGG C^CTAGG
BbvCI 1073 CCTCAGC CCTCAGC(-5/-2)
BciVI 973 GTATCC GTATCC(6/5)
BglI 663 GCCTGGGAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlnI 4487 CCTAGG C^CTAGG
BsaWI 283 ACCGGT [AT]^CCGG[AT]
Bsp1407I 4481 TGTACA T^GTACA
BspHI 1641 TCATGA T^CATGA
BspLU11I 4228 ACATGT A^CATGT
BsrGI 4481 TGTACA T^GTACA
Bst1107I 848 GTATAC GTA^TAC
BstEII 770 GGTGACC G^GT[ACGT]ACC
BstPI 770 GGTGACC G^GT[ACGT]ACC
BstUI 82 CGCG CG^CG
BstZ17I 848 GTATAC GTA^TAC
DraI 3838 TTTAAA TTT^AAA
EcoO65I 770 GGTGACC G^GT[ACGT]ACC
EcoRI 2103 GAATTC G^AATTC
HincII 3662 GTTAAC GT[CT]^[GA]AC
HindII 3662 GTTAAC GT[CT]^[GA]AC
HpaI 3662 GTTAAC GTT^AAC
KpnI 158 GGTACC GGTAC^C
MfeI 3332 CAATTG C^AATTG
MunI 3332 CAATTG C^AATTG
MvnI 82 CGCG CG^CG
NaeI 458 GCCGGC GCC^GGC
NdeI 4120 CATATG CA^TATG
NgoMIV 458 GCCGGC G^CCGGC
PflFI 884 GACACTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PinAI 283 ACCGGT A^CCGGT
RcaI 1641 TCATGA T^CATGA
SpeI 3984 ACTAGT A^CTAGT
SspBI 4481 TGTACA T^GTACA
Tth111I 884 GACACTGTC GAC[ACGT]^[ACGT][ACGT]GTC
XbaI 2981 TCTAGA T^CTAGA
XcmI 1468 CCAAACAGGGCGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 26 enzymes) #
###################################
Alw44I 906 GTGCAC G^TGCAC
3313 GTGCAC
ApaI 2238 GGGCCC GGGCC^C
4324 GGGCCC
ApaLI 906 GTGCAC G^TGCAC
3313 GTGCAC
AseI 1509 ATTAAT AT^TAAT
3280 ATTAAT
AsnI 1509 ATTAAT AT^TAAT
3280 ATTAAT
BamHI 1265 GGATCC G^GATCC
4381 GGATCC
BclI 691 TGATCA T^GATCA
4534 TGATCA
BcnI 155 CCCGG CC^[GC]GG
1034 CCGGG
BglII 340 AGATCT A^GATCT
3833 AGATCT
BlpI 110 GCTGAGC GC^T[ACGT]AGC
554 GCTGAGC
Bpu1102I 110 GCTGAGC GC^T[ACGT]AGC
554 GCTGAGC
BsrDI 436 CATTGC GCAATG(2/0)
1983 GCAATG
BstAPI 98 GCACTCCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
449 GCAGAAGCTGC
CelII 110 GCTGAGC GC^T[ACGT]AGC
554 GCTGAGC
EcoT22I 3210 ATGCAT ATGCA^T
3226 ATGCAT
FbaI 691 TGATCA T^GATCA
4534 TGATCA
HindIII 3059 AAGCTT A^AGCTT
3417 AAGCTT
MaeII 2227 ACGT A^CGT
2948 ACGT
NciI 155 CCCGG CC^[GC]GG
1034 CCGGG
NsiI 3210 ATGCAT ATGCA^T
3226 ATGCAT
PflMI 266 CCACATCATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
727 CCAAGGTGTGG
PshAI 33 GACCTCAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
3125 GACTGCTGTC
PshBI 1509 ATTAAT AT^TAAT
3280 ATTAAT
ScaI 236 AGTACT AGT^ACT
2661 AGTACT
SexAI 3520 ACCTGGT A^CC[AT]GGT
4009 ACCTGGT
Van91I 266 CCACATCATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
727 CCAAGGTGTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 107 enzymes) #
#########################################
AciI AcsI AfaI AflII AluI
AlwI AlwNI AocI ApoI Asp700I
AspHI AvaII BanI BanII BbsI
BbvI BfaI BfrI BmyI BpmI
BpuAI BsaI BsaJI BseRI BsgI
BsiHKAI BsiYI BslI BsmAI BsmFI
Bsp1286I BspMI BsrFI BsrI BssKI
BstF5I BstNI BstXI BstYI Bsu36I
Cac8I CfoI Cfr10I Cfr13I DdeI
DpnII DraII DsaI EarI Eco81I
EcoO109I EcoRII EcoT14I FauI Fnu4HI
FokI HaeIII HapII HhaI HinP1I
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeIII MboI MboII MflI
MnlI MseI MslI MspA1I MspI
MvaI MwoI NcoI NdeII NlaIII
NlaIV NspI PleI PstI PvuII
RsaI SacI SapI Sau3AI Sau96I
ScrFI SfaNI SfcI SmlI SphI
StuI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
XhoII XmnI