################################## # NO CUT ENZYMES ( 82 enzymes) # ################################## AatII AccIII AclI AcyI AhdI Aor51HI AscI AspEI AvaI AviII BalI BbeI BbrPI BcgI BsaAI BsaBI BsaHI BseAI BsiEI BsiWI BsmBI BsmI BsoBI BspDI BspEI BsrBI BssHII BssSI BstBI ClaI CpoI DraIII DrdI EaeI EagI Eam1105I EclXI Eco47III Eco52I EcoNI EcoRV FseI FspI HaeII HgaI Hin1I KasI KspI MamI MluI MluNI MroI MscI NarI NheI NotI NruI NspV PacI PaeR7I PmaCI PmeI PmlI PpuMI Psp1406I PvuI RsrII SacII SalI SbfI SfiI SfoI SfuI SgrAI SmaI SnaBI Sse8387I SspI SwaI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 43 enzymes) # ################################### Acc65I 158 GGTACC G^GTACC AccI 848 GTATAC GT^[AC][GT]AC AccII 82 CGCG CG^CG AflIII 4228 ACATGT A^C[GA][CT]GT AgeI 283 ACCGGT A^CCGGT Asp718I 158 GGTACC G^GTACC AspI 884 GACACTGTC GAC[ACGT]^[ACGT][ACGT]GTC AvrII 4487 CCTAGG C^CTAGG BbvCI 1073 CCTCAGC CCTCAGC(-5/-2) BciVI 973 GTATCC GTATCC(6/5) BglI 663 GCCTGGGAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BlnI 4487 CCTAGG C^CTAGG BsaWI 283 ACCGGT [AT]^CCGG[AT] Bsp1407I 4481 TGTACA T^GTACA BspHI 1641 TCATGA T^CATGA BspLU11I 4228 ACATGT A^CATGT BsrGI 4481 TGTACA T^GTACA Bst1107I 848 GTATAC GTA^TAC BstEII 770 GGTGACC G^GT[ACGT]ACC BstPI 770 GGTGACC G^GT[ACGT]ACC BstUI 82 CGCG CG^CG BstZ17I 848 GTATAC GTA^TAC DraI 3838 TTTAAA TTT^AAA EcoO65I 770 GGTGACC G^GT[ACGT]ACC EcoRI 2103 GAATTC G^AATTC HincII 3662 GTTAAC GT[CT]^[GA]AC HindII 3662 GTTAAC GT[CT]^[GA]AC HpaI 3662 GTTAAC GTT^AAC KpnI 158 GGTACC GGTAC^C MfeI 3332 CAATTG C^AATTG MunI 3332 CAATTG C^AATTG MvnI 82 CGCG CG^CG NaeI 458 GCCGGC GCC^GGC NdeI 4120 CATATG CA^TATG NgoMIV 458 GCCGGC G^CCGGC PflFI 884 GACACTGTC GAC[ACGT]^[ACGT][ACGT]GTC PinAI 283 ACCGGT A^CCGGT RcaI 1641 TCATGA T^CATGA SpeI 3984 ACTAGT A^CTAGT SspBI 4481 TGTACA T^GTACA Tth111I 884 GACACTGTC GAC[ACGT]^[ACGT][ACGT]GTC XbaI 2981 TCTAGA T^CTAGA XcmI 1468 CCAAACAGGGCGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG ################################### # TWO CUT ENZYMES ( 26 enzymes) # ################################### Alw44I 906 GTGCAC G^TGCAC 3313 GTGCAC ApaI 2238 GGGCCC GGGCC^C 4324 GGGCCC ApaLI 906 GTGCAC G^TGCAC 3313 GTGCAC AseI 1509 ATTAAT AT^TAAT 3280 ATTAAT AsnI 1509 ATTAAT AT^TAAT 3280 ATTAAT BamHI 1265 GGATCC G^GATCC 4381 GGATCC BclI 691 TGATCA T^GATCA 4534 TGATCA BcnI 155 CCCGG CC^[GC]GG 1034 CCGGG BglII 340 AGATCT A^GATCT 3833 AGATCT BlpI 110 GCTGAGC GC^T[ACGT]AGC 554 GCTGAGC Bpu1102I 110 GCTGAGC GC^T[ACGT]AGC 554 GCTGAGC BsrDI 436 CATTGC GCAATG(2/0) 1983 GCAATG BstAPI 98 GCACTCCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC 449 GCAGAAGCTGC CelII 110 GCTGAGC GC^T[ACGT]AGC 554 GCTGAGC EcoT22I 3210 ATGCAT ATGCA^T 3226 ATGCAT FbaI 691 TGATCA T^GATCA 4534 TGATCA HindIII 3059 AAGCTT A^AGCTT 3417 AAGCTT MaeII 2227 ACGT A^CGT 2948 ACGT NciI 155 CCCGG CC^[GC]GG 1034 CCGGG NsiI 3210 ATGCAT ATGCA^T 3226 ATGCAT PflMI 266 CCACATCATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 727 CCAAGGTGTGG PshAI 33 GACCTCAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC 3125 GACTGCTGTC PshBI 1509 ATTAAT AT^TAAT 3280 ATTAAT ScaI 236 AGTACT AGT^ACT 2661 AGTACT SexAI 3520 ACCTGGT A^CC[AT]GGT 4009 ACCTGGT Van91I 266 CCACATCATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 727 CCAAGGTGTGG ######################################### # 3 OR MORE CUT ENZYMES ( 107 enzymes) # ######################################### AciI AcsI AfaI AflII AluI AlwI AlwNI AocI ApoI Asp700I AspHI AvaII BanI BanII BbsI BbvI BfaI BfrI BmyI BpmI BpuAI BsaI BsaJI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI Bsp1286I BspMI BsrFI BsrI BssKI BstF5I BstNI BstXI BstYI Bsu36I Cac8I CfoI Cfr10I Cfr13I DdeI DpnII DraII DsaI EarI Eco81I EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeIII HapII HhaI HinP1I HinfI HpaII HphI ItaI Ksp632I MaeI MaeIII MboI MboII MflI MnlI MseI MslI MspA1I MspI MvaI MwoI NcoI NdeII NlaIII NlaIV NspI PleI PstI PvuII RsaI SacI SapI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI SphI StuI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I XhoII XmnI