##################################
# NO CUT ENZYMES ( 58 enzymes) #
##################################
AclI AscI AseI AsnI AvrII
BbrPI BbvCI BciVI BclI BlnI
BsaAI BsaBI BsiWI BsmBI BspDI
BspHI Bst1107I BstAPI BstBI BstEII
BstPI BstZ17I ClaI DraI EcoO65I
EcoRV EcoT22I FbaI HpaI MamI
MfeI MluI MunI NdeI NotI
NruI NsiI NspV PacI PaeR7I
PmaCI PmeI PmlI PshBI Psp1406I
PvuI RcaI SalI SbfI ScaI
SfiI SfuI SnaBI SpeI Sse8387I
SspI SwaI XhoI
###################################
# ONE CUT ENZYMES ( 42 enzymes) #
###################################
AatII 2002 GACGTC GACGT^C
AccI 2864 GTCTAC GT^[AC][GT]AC
AgeI 845 ACCGGT A^CCGGT
AhdI 2154 GACTCAGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 849 GTGCAC G^TGCAC
AocI 4472 CCTCAGG CC^T[ACGT]AGG
Aor51HI 1103 AGCGCT AGC^GCT
ApaLI 849 GTGCAC G^TGCAC
Asp700I 1723 GAATCCGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI 2154 GACTCAGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AviII 1063 TGCGCA TGC^GCA
BcgI 2986 CGACCAGTGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BglII 2034 AGATCT A^GATCT
BsaI 3610 GGTCTC GGTCTC(1/5)
Bsp1407I 2589 TGTACA T^GTACA
BspLU11I 4884 ACATGT A^CATGT
BsrDI 2417 CATTGC GCAATG(2/0)
BsrGI 2589 TGTACA T^GTACA
BssSI 2030 CACGAG CACGAG(-5/-1)
Bsu36I 4472 CCTCAGG CC^T[ACGT]AGG
CpoI 690 CGGACCG CG^G[AT]CCG
DraIII 647 CACGGTGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 1592 GACTTTCCTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eam1105I 2154 GACTCAGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 1103 AGCGCT AGC^GCT
Eco81I 4472 CCTCAGG CC^T[ACGT]AGG
EcoRI 4336 GAATTC G^AATTC
FspI 1063 TGCGCA TGC^GCA
HincII 546 GTCAAC GT[CT]^[GA]AC
HindII 546 GTCAAC GT[CT]^[GA]AC
PinAI 845 ACCGGT A^CCGGT
PshAI 3604 GACGCTGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
RsrII 690 CGGACCG CG^G[AT]CCG
SacI 3990 GAGCTC GAGCT^C
SapI 2854 GAAGAGC GCTCTTC(1/4)
SexAI 2637 ACCTGGT A^CC[AT]GGT
SgrAI 844 CACCGGTG C[GA]^CCGG[CT]G
SphI 271 GCATGC GCATG^C
SspBI 2589 TGTACA T^GTACA
StuI 2313 AGGCCT AGG^CCT
XbaI 4240 TCTAGA T^CTAGA
XmnI 1723 GAATCCGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 23 enzymes) #
###################################
Acc65I 1839 GGTACC G^GTACC
3288 GGTACC
AccIII 3105 TCCGGA T^CCGGA
3357 TCCGGA
AcsI 1519 GAATTT [GA]^AATT[CT]
4336 GAATTC
AflII 4207 CTTAAG C^TTAAG
4607 CTTAAG
AflIII 303 ACACGT A^C[GA][CT]GT
4884 ACATGT
ApoI 1519 GAATTT [GA]^AATT[CT]
4336 GAATTC
Asp718I 1839 GGTACC G^GTACC
3288 GGTACC
AspI 2636 GACCTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
3098 GACAGTGTC
BamHI 120 GGATCC G^GATCC
1211 GGATCC
BfrI 4207 CTTAAG C^TTAAG
4607 CTTAAG
BseAI 3105 TCCGGA T^CCGGA
3357 TCCGGA
BsmI 2011 GAATGC GAATGC(1/-1)
986 GCATTC
BspEI 3105 TCCGGA T^CCGGA
3357 TCCGGA
BssHII 1138 GCGCGC G^CGCGC
2622 GCGCGC
EcoNI 3124 CCTGGAGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
3834 CCTCCAGGAGG
FseI 1048 GGCCGGCC GGCCGG^CC
3550 GGCCGGCC
KpnI 1839 GGTACC GGTAC^C
3288 GGTACC
MroI 3105 TCCGGA T^CCGGA
3357 TCCGGA
NaeI 1049 GCCGGC GCC^GGC
3551 GCCGGC
NgoMIV 1049 GCCGGC G^CCGGC
3551 GCCGGC
PflFI 2636 GACCTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
3098 GACAGTGTC
PvuII 3761 CAGCTG CAG^CTG
4312 CAGCTG
Tth111I 2636 GACCTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
3098 GACAGTGTC
#########################################
# 3 OR MORE CUT ENZYMES ( 135 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
AlwI AlwNI ApaI AspHI AvaI
AvaII BalI BanI BanII BbeI
BbsI BbvI BcnI BfaI BglI
BlpI BmyI BpmI Bpu1102I BpuAI
BsaHI BsaJI BsaWI BseRI BsgI
BsiEI BsiHKAI BsiYI BslI BsmAI
BsmFI BsoBI Bsp1286I BspMI BsrBI
BsrFI BsrI BssKI BstF5I BstNI
BstUI BstXI BstYI Cac8I CelII
CfoI Cfr10I Cfr13I DdeI DpnII
DraII DsaI EaeI EagI EarI
EclXI Eco52I EcoO109I EcoRII EcoT14I
FauI Fnu4HI FokI HaeII HaeIII
HapII HgaI HhaI Hin1I HinP1I
HindIII HinfI HpaII HphI ItaI
KasI Ksp632I KspI MaeI MaeII
MaeIII MboI MboII MflI MluNI
MnlI MscI MseI MslI MspA1I
MspI MvaI MvnI MwoI NarI
NciI NcoI NdeII NheI NlaIII
NlaIV NspI PflMI PleI PpuMI
PstI RsaI SacII Sau3AI Sau96I
ScrFI SfaNI SfcI SfoI SmaI
SmlI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
Van91I XcmI XhoII XmaCI XmaI