##################################
# NO CUT ENZYMES ( 88 enzymes) #
##################################
AatII AccII AccIII AcyI AflII
AgeI AhdI Aor51HI AscI AspEI
AspI AvaI BalI BbeI BbrPI
BbvCI BfrI BglII BsaHI BsaWI
BseAI BsiEI BsiWI BsoBI BspDI
BspEI BsrBI BssHII BstBI BstUI
ClaI CpoI EagI Eam1105I EclXI
Eco47III Eco52I EcoRV EcoT22I FseI
HaeII Hin1I HpaI KasI KspI
MluI MluNI MroI MscI MvnI
NarI NcoI NheI NotI NruI
NsiI NspV PacI PaeR7I PflFI
PinAI PmaCI PmeI PmlI PshAI
PvuI RsrII SacI SacII SalI
SapI SbfI SexAI SfiI SfoI
SfuI SgrAI SmaI SnaBI SpeI
Sse8387I SwaI TaqI Tth111I TthHB8I
XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 50 enzymes) #
###################################
Acc65I 4757 GGTACC G^GTACC
AclI 2218 AACGTT AA^CGTT
AocI 286 CCTGAGG CC^T[ACGT]AGG
ApaI 2053 GGGCCC GGGCC^C
AseI 1566 ATTAAT AT^TAAT
AsnI 1566 ATTAAT AT^TAAT
Asp700I 3571 GAAAGACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 4757 GGTACC G^GTACC
AviII 4484 TGCGCA TGC^GCA
AvrII 4345 CCTAGG C^CTAGG
BamHI 3092 GGATCC G^GATCC
BanI 4757 GGTACC G^G[CT][GA]CC
BcgI 3617 GCAGCTTCTTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BglI 1356 GCCGCCACGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlnI 4345 CCTAGG C^CTAGG
BsaAI 1730 TACGTG [CT]AC^GT[GA]
BsaBI 4365 GATACTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI 854 GAGACC GGTCTC(1/5)
BsrFI 2777 GCCGGC [GA]^CCGG[CT]
BssSI 1336 CACGAG CACGAG(-5/-1)
BstEII 966 GGTTACC G^GT[ACGT]ACC
BstPI 966 GGTTACC G^GT[ACGT]ACC
Bsu36I 286 CCTGAGG CC^T[ACGT]AGG
CfoI 4485 GCGC GCG^C
Cfr10I 2777 GCCGGC [GA]^CCGG[CT]
DraIII 1926 CACAGAGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 3657 GACGGAGAAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI 1360 CCACGG C^C[GA][CT]GG
EaeI 2826 CGGCCA [CT]^GGCC[GA]
Eco81I 286 CCTGAGG CC^T[ACGT]AGG
EcoO65I 966 GGTTACC G^GT[ACGT]ACC
EcoRI 3099 GAATTC G^AATTC
FauI 1443 GCGGG CCCGC(4/6)
FspI 4484 TGCGCA TGC^GCA
HhaI 4485 GCGC GCG^C
HinP1I 4485 GCGC G^CGC
KpnI 4757 GGTACC GGTAC^C
MamI 4365 GATACTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 2540 CAATTG C^AATTG
MspA1I 4898 CAGCTG C[AC]G^C[GT]G
MunI 2540 CAATTG C^AATTG
NaeI 2777 GCCGGC GCC^GGC
NgoMIV 2777 GCCGGC G^CCGGC
PshBI 1566 ATTAAT AT^TAAT
Psp1406I 2218 AACGTT AA^CGTT
PvuII 4898 CAGCTG CAG^CTG
SphI 1439 GCATGC GCATG^C
XbaI 2262 TCTAGA T^CTAGA
XcmI 40 CCATTAGCTGTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmnI 3571 GAAAGACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 29 enzymes) #
###################################
Alw44I 727 GTGCAC G^TGCAC
1967 GTGCAC
AlwNI 805 CAGTTGCTG CAG[ACGT][ACGT][ACGT]^CTG
1424 CAGAAGCTG
ApaLI 727 GTGCAC G^TGCAC
1967 GTGCAC
BanII 178 GAGCCC G[GA]GC[CT]^C
2053 GGGCCC
BbsI 2546 GAAGAC GAAGAC(2/6)
1386 GTCTTC
BciVI 1706 GGATAC GTATCC(6/5)
4300 GTATCC
BcnI 181 CCCGG CC^[GC]GG
2042 CCCGG
BpuAI 2546 GAAGAC GAAGAC(2/6)
1386 GTCTTC
BseRI 405 CTCCTC GAGGAG(10/8)
2873 CTCCTC
BsgI 2118 CTGCAC GTGCAG(16/14)
4042 CTGCAC
BsmBI 1823 CGTCTC CGTCTC(1/5)
700 GAGACG
Bsp1407I 1208 TGTACA T^GTACA
2909 TGTACA
BspHI 678 TCATGA T^CATGA
3894 TCATGA
BsrDI 87 CATTGC GCAATG(2/0)
164 GCAATG
BsrGI 1208 TGTACA T^GTACA
2909 TGTACA
Bst1107I 535 GTATAC GTA^TAC
3359 GTATAC
BstAPI 1318 GCACAGGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
1969 GCACATTTTGC
BstYI 520 GGATCT [GA]^GATC[CT]
3092 GGATCC
BstZ17I 535 GTATAC GTA^TAC
3359 GTATAC
EcoNI 2767 CCTGATGCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
3607 CCTGTCGTAGG
MflI 520 GGATCT [GA]^GATC[CT]
3092 GGATCC
NciI 181 CCCGG CC^[GC]GG
2042 CCCGG
NdeI 142 CATATG CA^TATG
491 CATATG
PpuMI 1268 AGGACCT [GA]G^G[AT]CC[CT]
2681 GGGACCT
RcaI 678 TCATGA T^CATGA
3894 TCATGA
ScaI 2819 AGTACT AGT^ACT
4156 AGTACT
SspBI 1208 TGTACA T^GTACA
2909 TGTACA
StuI 2795 AGGCCT AGG^CCT
4290 AGGCCT
XhoII 520 GGATCT [GA]^GATC[CT]
3092 GGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 91 enzymes) #
#########################################
AccI AciI AcsI AfaI AflIII
AluI AlwI ApoI AspHI AvaII
BbvI BclI BfaI BlpI BmyI
BpmI Bpu1102I BsaJI BsiHKAI BsiYI
BslI BsmAI BsmFI BsmI Bsp1286I
BspLU11I BspMI BsrI BssKI BstF5I
BstNI BstXI Cac8I CelII Cfr13I
DdeI DpnII DraI DraII EarI
EcoO109I EcoRII EcoT14I FbaI Fnu4HI
FokI HaeIII HapII HgaI HincII
HindII HindIII HinfI HpaII HphI
ItaI Ksp632I MaeI MaeII MaeIII
MboI MboII MnlI MseI MslI
MspI MvaI MwoI NdeII NlaIII
NlaIV NspI PflMI PleI PstI
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI SmlI SspI StyI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
Van91I