##################################
# NO CUT ENZYMES (   84 enzymes) #
##################################

AatII       AccII       AccIII      AcyI        AflIII      
AgeI        AhdI        ApaI        AscI        AseI        
AsnI        AspEI       AspI        AvrII       BamHI       
BbeI        BbrPI       BbvCI       BglI        BlnI        
BsaAI       BsaBI       BsaHI       BsaWI       BseAI       
BsiEI       BsiWI       BspDI       BspEI       BspLU11I    
BsrBI       BssHII      BssSI       BstBI       BstUI       
ClaI        CpoI        DsaI        EagI        Eam1105I    
EclXI       Eco52I      EcoRI       FseI        Hin1I       
HindIII     KasI        KspI        MamI        MfeI        
MluI        MroI        MunI        MvnI        NarI        
NcoI        NotI        NruI        NspV        PacI        
PaeR7I      PflFI       PinAI       PmaCI       PmeI        
PmlI        PpuMI       PshAI       PshBI       PvuI        
RsrII       SacII       SalI        SapI        SfiI        
SfoI        SfuI        SgrAI       SnaBI       StuI        
SwaI        Tth111I     XbaI        XhoI        


###################################
# ONE CUT ENZYMES (   60 enzymes) #
###################################

Acc65I       4225  GGTACC               G^GTACC
AclI         2134  AACGTT               AA^CGTT
AflII        1641  CTTAAG               C^TTAAG
Alw44I       1962  GTGCAC               G^TGCAC
AocI         3670  CCTGAGG              CC^T[ACGT]AGG
Aor51HI      1477  AGCGCT               AGC^GCT
ApaLI        1962  GTGCAC               G^TGCAC
Asp700I      1167  GAAAGTATTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I      4225  GGTACC               G^GTACC
AvaI          285  CCCGGG               C^[CT]CG[GA]G
BanI         4225  GGTACC               G^G[CT][GA]CC
BciVI        3311  GTATCC               GTATCC(6/5)
BclI         2012  TGATCA               T^GATCA
BfrI         1641  CTTAAG               C^TTAAG
BsmBI         417  GAGACG               CGTCTC(1/5)
BsoBI         285  CCCGGG               C^[CT]CG[GA]G
BspHI         144  TCATGA               T^CATGA
BstAPI       1350  GCAAATGGTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII        219  GGTCACC              G^GT[ACGT]ACC
BstPI         219  GGTCACC              G^GT[ACGT]ACC
BstXI        4211  CCATCTGATTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I       3670  CCTGAGG              CC^T[ACGT]AGG
DraII        1462  AGGGCCT              [GA]G^G[ACGT]CC[CT]
DraIII        742  CACCAGGTG            CAC[ACGT][ACGT][ACGT]^GTG
DrdI         4662  GACACTGTAGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI         2794  CTCTTC               CTCTTC(1/4)
Eco47III     1477  AGCGCT               AGC^GCT
Eco81I       3670  CCTGAGG              CC^T[ACGT]AGG
EcoO109I     1462  AGGGCCT              [GA]G^G[ACGT]CC[CT]
EcoO65I       219  GGTCACC              G^GT[ACGT]ACC
EcoRV         326  GATATC               GAT^ATC
EcoT14I      3389  CCTTGG               C^C[AT][AT]GG
EcoT22I      4303  ATGCAT               ATGCA^T
FauI         3366  CCCGC                CCCGC(4/6)
FbaI         2012  TGATCA               T^GATCA
HgaI          595  GCGTC                GACGC(5/10)
HincII        272  GTTAAC               GT[CT]^[GA]AC
HindII        272  GTTAAC               GT[CT]^[GA]AC
HpaI          272  GTTAAC               GTT^AAC
KpnI         4225  GGTACC               GGTAC^C
Ksp632I      2794  CTCTTC               CTCTTC(1/4)
MspA1I       1604  CAGCTG               C[AC]G^C[GT]G
NaeI         3661  GCCGGC               GCC^GGC
NdeI         3096  CATATG               CA^TATG
NgoMIV       3661  GCCGGC               G^CCGGC
NsiI         4303  ATGCAT               ATGCA^T
NspI         3486  GCATGC               [GA]CATG^[CT]
Psp1406I     2134  AACGTT               AA^CGTT
PvuII        1604  CAGCTG               CAG^CTG
RcaI          144  TCATGA               T^CATGA
SbfI         3102  CCTGCAGG             CCTGCA^GG
SexAI         743  ACCAGGT              A^CC[AT]GGT
SmaI          285  CCCGGG               CCC^GGG
SphI         3486  GCATGC               GCATG^C
Sse8387I     3102  CCTGCAGG             CCTGCA^GG
SspI         3152  AATATT               AAT^ATT
StyI         3389  CCTTGG               C^C[AT][AT]GG
XmaCI         285  CCCGGG               C^CCGGG
XmaI          285  CCCGGG               C^CCGGG
XmnI         1167  GAAAGTATTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   24 enzymes) #
###################################

AlwNI         986  CAGAGCCTG            CAG[ACGT][ACGT][ACGT]^CTG
             3509  CAGGAGCTG           
AviII        3473  TGCGCA               TGC^GCA
             3483  TGCGCA              
BalI          940  TGGCCA               TGG^CCA
             3076  TGGCCA              
BanII         794  GAGCTC               G[GA]GC[CT]^C
             1952  GAGCTC              
BcgI          866  CGAACCTGCTGC         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
             1340  CGATGATTTTGC        
BglII        1259  AGATCT               A^GATCT
             4795  AGATCT              
BlpI         1990  GCTTAGC              GC^T[ACGT]AGC
             4647  GCTCAGC             
Bpu1102I     1990  GCTTAGC              GC^T[ACGT]AGC
             4647  GCTCAGC             
BsaI         1008  GAGACC               GGTCTC(1/5)
             1389  GAGACC              
BspMI         869  ACCTGC               ACCTGC(4/8)
             2050  GCAGGT              
BsrDI        1577  CATTGC               GCAATG(2/0)
             1783  GCAATG              
BsrFI        2020  GCCGGT               [GA]^CCGG[CT]
             3661  GCCGGC              
CelII        1990  GCTTAGC              GC^T[ACGT]AGC
             4647  GCTCAGC             
Cfr10I       2020  GCCGGT               [GA]^CCGG[CT]
             3661  GCCGGC              
EcoNI        1058  CCTGTTCGAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
             3670  CCTGAGGGAGG         
FspI         3473  TGCGCA               TGC^GCA
             3483  TGCGCA              
HaeII        1477  AGCGCT               [GA]GCGC^[CT]
             1522  GGCGCT              
MluNI         940  TGGCCA               TGG^CCA
             3076  TGGCCA              
MscI          940  TGGCCA               TGG^CCA
             3076  TGGCCA              
NheI         4096  GCTAGC               G^CTAGC
             4529  GCTAGC              
PflMI         741  CCACCAGGTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             3377  CCAGAGTGTGG         
SacI          794  GAGCTC               GAGCT^C
             1952  GAGCTC              
ScaI          172  AGTACT               AGT^ACT
             1681  AGTACT              
Van91I        741  CCACCAGGTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             3377  CCAGAGTGTGG         


#########################################
# 3 OR MORE CUT ENZYMES (   90 enzymes) #
#########################################

AccI        AciI        AcsI        AfaI        AluI        
AlwI        ApoI        AspHI       AvaII       BbsI        
BbvI        BcnI        BfaI        BmyI        BpmI        
BpuAI       BsaJI       BseRI       BsgI        BsiHKAI     
BsiYI       BslI        BsmAI       BsmFI       BsmI        
Bsp1286I    Bsp1407I    BsrGI       BsrI        BssKI       
Bst1107I    BstF5I      BstNI       BstYI       BstZ17I     
Cac8I       CfoI        Cfr13I      DdeI        DpnII       
DraI        EaeI        EcoRII      Fnu4HI      FokI        
HaeIII      HapII       HhaI        HinP1I      HinfI       
HpaII       HphI        ItaI        MaeI        MaeII       
MaeIII      MboI        MboII       MflI        MnlI        
MseI        MslI        MspI        MvaI        MwoI        
NciI        NdeII       NlaIII      NlaIV       PleI        
PstI        RsaI        Sau3AI      Sau96I      ScrFI       
SfaNI       SfcI        SmlI        SpeI        SspBI       
TaqI        TfiI        Tru9I       TseI        Tsp45I      
Tsp509I     TspRI       TthHB8I     XcmI        XhoII