##################################
# NO CUT ENZYMES (   84 enzymes) #
##################################

Acc65I      AccI        AccII       AclI        AgeI        
AhdI        Alw44I      Aor51HI     ApaI        ApaLI       
AscI        AseI        AsnI        Asp718I     AspEI       
BanI        BbeI        BbrPI       BclI        BlpI        
Bpu1102I    BsaAI       BsiEI       BsiWI       BspDI       
BssHII      Bst1107I    BstAPI      BstBI       BstUI       
BstZ17I     CelII       ClaI        CpoI        DraII       
DraIII      DrdI        EagI        Eam1105I    EclXI       
Eco47III    Eco52I      EcoNI       EcoO109I    EcoRV       
FbaI        FseI        KasI        KpnI        KspI        
MluI        MvnI        NaeI        NarI        NcoI        
NdeI        NgoMIV      NotI        NruI        NspV        
PacI        PinAI       PmaCI       PmeI        PmlI        
PpuMI       PshBI       Psp1406I    PvuI        RsrII       
SacI        SacII       SalI        SbfI        SexAI       
SfoI        SfuI        SgrAI       SnaBI       SpeI        
Sse8387I    StuI        SwaI        XbaI        


###################################
# ONE CUT ENZYMES (   39 enzymes) #
###################################

AatII        2846  GACGTC               GACGT^C
AccIII       3148  TCCGGA               T^CCGGA
AcyI         2846  GACGTC               G[GA]^CG[CT]C
AflIII       2526  ACATGT               A^C[GA][CT]GT
AocI         2720  CCTGAGG              CC^T[ACGT]AGG
AviII        4140  TGCGCA               TGC^GCA
BamHI        3299  GGATCC               G^GATCC
BanII        1941  GAGCCC               G[GA]GC[CT]^C
BcgI          191  CGAACTTCATGC         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI         545  GTATCC               GTATCC(6/5)
BglI         4320  GCCTTGAAGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII        2683  AGATCT               A^GATCT
BsaHI        2846  GACGTC               G[GA]^CG[CT]C
BsaWI        3148  TCCGGA               [AT]^CCGG[AT]
BseAI        3148  TCCGGA               T^CCGGA
BsmBI        2848  CGTCTC               CGTCTC(1/5)
BsmI         4479  GCATTC               GAATGC(1/-1)
BspEI        3148  TCCGGA               T^CCGGA
BspLU11I     2526  ACATGT               A^CATGT
BsrBI        1190  CCGCTC               CCGCTC(-3/-3)
Bsu36I       2720  CCTGAGG              CC^T[ACGT]AGG
DsaI         1184  CCGTGG               C^C[GA][CT]GG
Eco81I       2720  CCTGAGG              CC^T[ACGT]AGG
FauI          900  GCGGG                CCCGC(4/6)
FspI         4140  TGCGCA               TGC^GCA
HaeII        2122  AGCGCC               [GA]GCGC^[CT]
Hin1I        2846  GACGTC               G[GA]^CG[CT]C
HpaI         2669  GTTAAC               GTT^AAC
MroI         3148  TCCGGA               T^CCGGA
MslI         4767  CACCAAGATG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NheI         2119  GCTAGC               G^CTAGC
PaeR7I       3166  CTCGAG               C^TCGAG
PshAI         109  GACTATTGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SfiI         4319  GGCCTTGAAGGCC        GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SmaI         4095  CCCGGG               CCC^GGG
XcmI          783  CCATTATAGTAATGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI         3166  CTCGAG               C^TCGAG
XmaCI        4095  CCCGGG               C^CCGGG
XmaI         4095  CCCGGG               C^CCGGG


###################################
# TWO CUT ENZYMES (   31 enzymes) #
###################################

AflII        2376  CTTAAG               C^TTAAG
             4484  CTTAAG              
AlwNI         571  CAGTACCTG            CAG[ACGT][ACGT][ACGT]^CTG
             2011  CAGGGTCTG           
Asp700I      3575  GAACACTTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
             5323  GAATATTTTC          
AspI         2927  GACAATGTC            GAC[ACGT]^[ACGT][ACGT]GTC
             3979  GACAAAGTC           
AvrII        2041  CCTAGG               C^CTAGG
             2564  CCTAGG              
BfrI         2376  CTTAAG               C^TTAAG
             4484  CTTAAG              
BlnI         2041  CCTAGG               C^CTAGG
             2564  CCTAGG              
BsaBI        1792  GATCACGATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
             4079  GATGCCAATC          
BsgI         3217  CTGCAC               GTGCAG(16/14)
              746  GTGCAG              
BspMI        3215  ACCTGC               ACCTGC(4/8)
             3291  ACCTGC              
BsrFI        1862  ACCGGC               [GA]^CCGG[CT]
             4274  ACCGGC              
BssSI        1311  CACGAG               CACGAG(-5/-1)
             3315  CTCGTG              
BstEII       1522  GGTGACC              G^GT[ACGT]ACC
             3324  GGTTACC             
BstPI        1522  GGTGACC              G^GT[ACGT]ACC
             3324  GGTTACC             
BstXI        3801  CCAAGCAGTTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             3858  CCAAAAGTCTGG        
CfoI         2123  GCGC                 GCG^C
             4141  GCGC                
Cfr10I       1862  ACCGGC               [GA]^CCGG[CT]
             4274  ACCGGC              
EcoO65I      1522  GGTGACC              G^GT[ACGT]ACC
             3324  GGTTACC             
HgaI         2111  GACGC                GACGC(5/10)
             2760  GCGTC               
HhaI         2123  GCGC                 GCG^C
             4141  GCGC                
HinP1I       2123  GCGC                 G^CGC
             4141  GCGC                
HindIII      4025  AAGCTT               A^AGCTT
             5312  AAGCTT              
MaeII        2847  ACGT                 A^CGT
             4374  ACGT                
MamI         1792  GATCACGATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
             4079  GATGCCAATC          
MfeI         1461  CAATTG               C^AATTG
             2599  CAATTG              
MunI         1461  CAATTG               C^AATTG
             2599  CAATTG              
PflFI        2927  GACAATGTC            GAC[ACGT]^[ACGT][ACGT]GTC
             3979  GACAAAGTC           
PvuII        1105  CAGCTG               CAG^CTG
             2546  CAGCTG              
SapI         3367  GAAGAGC              GCTCTTC(1/4)
             4358  GCTCTTC             
Tth111I      2927  GACAATGTC            GAC[ACGT]^[ACGT][ACGT]GTC
             3979  GACAAAGTC           
XmnI         3575  GAACACTTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
             5323  GAATATTTTC          


#########################################
# 3 OR MORE CUT ENZYMES (  104 enzymes) #
#########################################

AciI        AcsI        AfaI        AluI        AlwI        
ApoI        AspHI       AvaI        AvaII       BalI        
BbsI        BbvCI       BbvI        BcnI        BfaI        
BmyI        BpmI        BpuAI       BsaI        BsaJI       
BseRI       BsiHKAI     BsiYI       BslI        BsmAI       
BsmFI       BsoBI       Bsp1286I    Bsp1407I    BspHI       
BsrDI       BsrGI       BsrI        BssKI       BstF5I      
BstNI       BstYI       Cac8I       Cfr13I      DdeI        
DpnII       DraI        EaeI        EarI        EcoRI       
EcoRII      EcoT14I     EcoT22I     Fnu4HI      FokI        
HaeIII      HapII       HincII      HindII      HinfI       
HpaII       HphI        ItaI        Ksp632I     MaeI        
MaeIII      MboI        MboII       MflI        MluNI       
MnlI        MscI        MseI        MspA1I      MspI        
MvaI        MwoI        NciI        NdeII       NlaIII      
NlaIV       NsiI        NspI        PflMI       PleI        
PstI        RcaI        RsaI        Sau3AI      Sau96I      
ScaI        ScrFI       SfaNI       SfcI        SmlI        
SphI        SspBI       SspI        StyI        TaqI        
TfiI        Tru9I       TseI        Tsp45I      Tsp509I     
TspRI       TthHB8I     Van91I      XhoII