##################################
# NO CUT ENZYMES ( 84 enzymes) #
##################################
Acc65I AccI AccII AclI AgeI
AhdI Alw44I Aor51HI ApaI ApaLI
AscI AseI AsnI Asp718I AspEI
BanI BbeI BbrPI BclI BlpI
Bpu1102I BsaAI BsiEI BsiWI BspDI
BssHII Bst1107I BstAPI BstBI BstUI
BstZ17I CelII ClaI CpoI DraII
DraIII DrdI EagI Eam1105I EclXI
Eco47III Eco52I EcoNI EcoO109I EcoRV
FbaI FseI KasI KpnI KspI
MluI MvnI NaeI NarI NcoI
NdeI NgoMIV NotI NruI NspV
PacI PinAI PmaCI PmeI PmlI
PpuMI PshBI Psp1406I PvuI RsrII
SacI SacII SalI SbfI SexAI
SfoI SfuI SgrAI SnaBI SpeI
Sse8387I StuI SwaI XbaI
###################################
# ONE CUT ENZYMES ( 39 enzymes) #
###################################
AatII 2846 GACGTC GACGT^C
AccIII 3148 TCCGGA T^CCGGA
AcyI 2846 GACGTC G[GA]^CG[CT]C
AflIII 2526 ACATGT A^C[GA][CT]GT
AocI 2720 CCTGAGG CC^T[ACGT]AGG
AviII 4140 TGCGCA TGC^GCA
BamHI 3299 GGATCC G^GATCC
BanII 1941 GAGCCC G[GA]GC[CT]^C
BcgI 191 CGAACTTCATGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 545 GTATCC GTATCC(6/5)
BglI 4320 GCCTTGAAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 2683 AGATCT A^GATCT
BsaHI 2846 GACGTC G[GA]^CG[CT]C
BsaWI 3148 TCCGGA [AT]^CCGG[AT]
BseAI 3148 TCCGGA T^CCGGA
BsmBI 2848 CGTCTC CGTCTC(1/5)
BsmI 4479 GCATTC GAATGC(1/-1)
BspEI 3148 TCCGGA T^CCGGA
BspLU11I 2526 ACATGT A^CATGT
BsrBI 1190 CCGCTC CCGCTC(-3/-3)
Bsu36I 2720 CCTGAGG CC^T[ACGT]AGG
DsaI 1184 CCGTGG C^C[GA][CT]GG
Eco81I 2720 CCTGAGG CC^T[ACGT]AGG
FauI 900 GCGGG CCCGC(4/6)
FspI 4140 TGCGCA TGC^GCA
HaeII 2122 AGCGCC [GA]GCGC^[CT]
Hin1I 2846 GACGTC G[GA]^CG[CT]C
HpaI 2669 GTTAAC GTT^AAC
MroI 3148 TCCGGA T^CCGGA
MslI 4767 CACCAAGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NheI 2119 GCTAGC G^CTAGC
PaeR7I 3166 CTCGAG C^TCGAG
PshAI 109 GACTATTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SfiI 4319 GGCCTTGAAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SmaI 4095 CCCGGG CCC^GGG
XcmI 783 CCATTATAGTAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 3166 CTCGAG C^TCGAG
XmaCI 4095 CCCGGG C^CCGGG
XmaI 4095 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 31 enzymes) #
###################################
AflII 2376 CTTAAG C^TTAAG
4484 CTTAAG
AlwNI 571 CAGTACCTG CAG[ACGT][ACGT][ACGT]^CTG
2011 CAGGGTCTG
Asp700I 3575 GAACACTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
5323 GAATATTTTC
AspI 2927 GACAATGTC GAC[ACGT]^[ACGT][ACGT]GTC
3979 GACAAAGTC
AvrII 2041 CCTAGG C^CTAGG
2564 CCTAGG
BfrI 2376 CTTAAG C^TTAAG
4484 CTTAAG
BlnI 2041 CCTAGG C^CTAGG
2564 CCTAGG
BsaBI 1792 GATCACGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
4079 GATGCCAATC
BsgI 3217 CTGCAC GTGCAG(16/14)
746 GTGCAG
BspMI 3215 ACCTGC ACCTGC(4/8)
3291 ACCTGC
BsrFI 1862 ACCGGC [GA]^CCGG[CT]
4274 ACCGGC
BssSI 1311 CACGAG CACGAG(-5/-1)
3315 CTCGTG
BstEII 1522 GGTGACC G^GT[ACGT]ACC
3324 GGTTACC
BstPI 1522 GGTGACC G^GT[ACGT]ACC
3324 GGTTACC
BstXI 3801 CCAAGCAGTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3858 CCAAAAGTCTGG
CfoI 2123 GCGC GCG^C
4141 GCGC
Cfr10I 1862 ACCGGC [GA]^CCGG[CT]
4274 ACCGGC
EcoO65I 1522 GGTGACC G^GT[ACGT]ACC
3324 GGTTACC
HgaI 2111 GACGC GACGC(5/10)
2760 GCGTC
HhaI 2123 GCGC GCG^C
4141 GCGC
HinP1I 2123 GCGC G^CGC
4141 GCGC
HindIII 4025 AAGCTT A^AGCTT
5312 AAGCTT
MaeII 2847 ACGT A^CGT
4374 ACGT
MamI 1792 GATCACGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
4079 GATGCCAATC
MfeI 1461 CAATTG C^AATTG
2599 CAATTG
MunI 1461 CAATTG C^AATTG
2599 CAATTG
PflFI 2927 GACAATGTC GAC[ACGT]^[ACGT][ACGT]GTC
3979 GACAAAGTC
PvuII 1105 CAGCTG CAG^CTG
2546 CAGCTG
SapI 3367 GAAGAGC GCTCTTC(1/4)
4358 GCTCTTC
Tth111I 2927 GACAATGTC GAC[ACGT]^[ACGT][ACGT]GTC
3979 GACAAAGTC
XmnI 3575 GAACACTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
5323 GAATATTTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 104 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
ApoI AspHI AvaI AvaII BalI
BbsI BbvCI BbvI BcnI BfaI
BmyI BpmI BpuAI BsaI BsaJI
BseRI BsiHKAI BsiYI BslI BsmAI
BsmFI BsoBI Bsp1286I Bsp1407I BspHI
BsrDI BsrGI BsrI BssKI BstF5I
BstNI BstYI Cac8I Cfr13I DdeI
DpnII DraI EaeI EarI EcoRI
EcoRII EcoT14I EcoT22I Fnu4HI FokI
HaeIII HapII HincII HindII HinfI
HpaII HphI ItaI Ksp632I MaeI
MaeIII MboI MboII MflI MluNI
MnlI MscI MseI MspA1I MspI
MvaI MwoI NciI NdeII NlaIII
NlaIV NsiI NspI PflMI PleI
PstI RcaI RsaI Sau3AI Sau96I
ScaI ScrFI SfaNI SfcI SmlI
SphI SspBI SspI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I Van91I XhoII