################################## # NO CUT ENZYMES ( 83 enzymes) # ################################## Acc65I AccI AccIII AclI AflII AgeI AhdI AscI AseI AsnI Asp718I AspEI AviII BamHI BbrPI BciVI BclI BfrI BseAI BsiEI BsiWI BsmBI Bsp1407I BspDI BspEI BspHI BsrGI Bst1107I BstBI BstEII BstPI BstZ17I ClaI CpoI DraI DraIII EagI Eam1105I EclXI Eco52I EcoO65I EcoRV EcoT22I FbaI FspI HindIII HpaI KpnI MfeI MluI MroI MunI NdeI NotI NruI NsiI NspV PacI PinAI PmaCI PmeI PmlI PshBI Psp1406I PvuI RcaI RsrII SalI SapI SbfI SexAI SfuI SgrAI SmaI SnaBI SpeI Sse8387I SspBI SspI SwaI XbaI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 43 enzymes) # ################################### AatII 729 GACGTC GACGT^C AlwI 947 GATCC GGATC(4/5) AocI 2096 CCTGAGG CC^T[ACGT]AGG Aor51HI 1858 AGCGCT AGC^GCT AspI 684 GACCATGTC GAC[ACGT]^[ACGT][ACGT]GTC AvrII 237 CCTAGG C^CTAGG BalI 477 TGGCCA TGG^CCA BbsI 290 GAAGAC GAAGAC(2/6) BlnI 237 CCTAGG C^CTAGG BpuAI 290 GAAGAC GAAGAC(2/6) BsaAI 1266 TACGTG [CT]AC^GT[GA] BsaBI 1307 GATTGACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsaWI 1340 TCCGGT [AT]^CCGG[AT] BsmI 1958 GAATGC GAATGC(1/-1) BspLU11I 1030 ACATGT A^CATGT BsrDI 3155 GCAATG GCAATG(2/0) BssHII 1022 GCGCGC G^CGCGC BssSI 3551 CTCGTG CACGAG(-5/-1) BstAPI 1946 GCAGGCCATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstXI 166 CCACTGACATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG Bsu36I 2096 CCTGAGG CC^T[ACGT]AGG Eco47III 1858 AGCGCT AGC^GCT Eco81I 2096 CCTGAGG CC^T[ACGT]AGG FseI 2592 GGCCGGCC GGCCGG^CC HincII 2183 GTTGAC GT[CT]^[GA]AC HindII 2183 GTTGAC GT[CT]^[GA]AC KspI 844 CCGCGG CCGC^GG MamI 1307 GATTGACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MluNI 477 TGGCCA TGG^CCA MscI 477 TGGCCA TGG^CCA MseI 3772 TTAA T^TAA NcoI 694 CCATGG C^CATGG NheI 2083 GCTAGC G^CTAGC PaeR7I 1594 CTCGAG C^TCGAG PflFI 684 GACCATGTC GAC[ACGT]^[ACGT][ACGT]GTC PshAI 929 GACCAACGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC SacII 844 CCGCGG CCGC^GG ScaI 1204 AGTACT AGT^ACT SfiI 2805 GGCCCACTCGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC SphI 1439 GCATGC GCATG^C Tru9I 3772 TTAA T^TAA Tth111I 684 GACCATGTC GAC[ACGT]^[ACGT][ACGT]GTC XhoI 1594 CTCGAG C^TCGAG ################################### # TWO CUT ENZYMES ( 9 enzymes) # ################################### Alw44I 354 GTGCAC G^TGCAC 1832 GTGCAC ApaLI 354 GTGCAC G^TGCAC 1832 GTGCAC BbvCI 950 CCTCAGC CCTCAGC(-5/-2) 821 GCTGAGG BcgI 1151 CGAGCTGGGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) 1499 CGACAAGATTGC BglI 858 GCCCCTCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC 2806 GCCCACTCGGC DrdI 522 GACACAGCTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 3373 GACGGTGCAGTC EcoNI 40 CCTGGCTCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG 1873 CCTTTGCCAGG EcoRI 3254 GAATTC G^AATTC 3584 GAATTC HgaI 855 GACGC GACGC(5/10) 2609 GACGC ######################################### # 3 OR MORE CUT ENZYMES ( 123 enzymes) # ######################################### AccII AciI AcsI AcyI AfaI AflIII AluI AlwNI ApaI ApoI Asp700I AspHI AvaI AvaII BanI BanII BbeI BbvI BcnI BfaI BglII BlpI BmyI BpmI Bpu1102I BsaHI BsaI BsaJI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I BspMI BsrBI BsrFI BsrI BssKI BstF5I BstNI BstUI BstYI Cac8I CelII CfoI Cfr10I Cfr13I DdeI DpnII DraII DsaI EaeI EarI EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeII HaeIII HapII HhaI Hin1I HinP1I HinfI HpaII HphI ItaI KasI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MnlI MslI MspA1I MspI MvaI MvnI MwoI NaeI NarI NciI NdeII NgoMIV NlaIII NlaIV NspI PflMI PleI PpuMI PstI PvuII RsaI SacI Sau3AI Sau96I ScrFI SfaNI SfcI SfoI SmlI StuI StyI TaqI TfiI TseI Tsp45I Tsp509I TspRI TthHB8I Van91I XcmI XhoII XmnI