##################################
# NO CUT ENZYMES (   83 enzymes) #
##################################

Acc65I      AccI        AccIII      AclI        AflII       
AgeI        AhdI        AscI        AseI        AsnI        
Asp718I     AspEI       AviII       BamHI       BbrPI       
BciVI       BclI        BfrI        BseAI       BsiEI       
BsiWI       BsmBI       Bsp1407I    BspDI       BspEI       
BspHI       BsrGI       Bst1107I    BstBI       BstEII      
BstPI       BstZ17I     ClaI        CpoI        DraI        
DraIII      EagI        Eam1105I    EclXI       Eco52I      
EcoO65I     EcoRV       EcoT22I     FbaI        FspI        
HindIII     HpaI        KpnI        MfeI        MluI        
MroI        MunI        NdeI        NotI        NruI        
NsiI        NspV        PacI        PinAI       PmaCI       
PmeI        PmlI        PshBI       Psp1406I    PvuI        
RcaI        RsrII       SalI        SapI        SbfI        
SexAI       SfuI        SgrAI       SmaI        SnaBI       
SpeI        Sse8387I    SspBI       SspI        SwaI        
XbaI        XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   43 enzymes) #
###################################

AatII         729  GACGTC               GACGT^C
AlwI          947  GATCC                GGATC(4/5)
AocI         2096  CCTGAGG              CC^T[ACGT]AGG
Aor51HI      1858  AGCGCT               AGC^GCT
AspI          684  GACCATGTC            GAC[ACGT]^[ACGT][ACGT]GTC
AvrII         237  CCTAGG               C^CTAGG
BalI          477  TGGCCA               TGG^CCA
BbsI          290  GAAGAC               GAAGAC(2/6)
BlnI          237  CCTAGG               C^CTAGG
BpuAI         290  GAAGAC               GAAGAC(2/6)
BsaAI        1266  TACGTG               [CT]AC^GT[GA]
BsaBI        1307  GATTGACATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI        1340  TCCGGT               [AT]^CCGG[AT]
BsmI         1958  GAATGC               GAATGC(1/-1)
BspLU11I     1030  ACATGT               A^CATGT
BsrDI        3155  GCAATG               GCAATG(2/0)
BssHII       1022  GCGCGC               G^CGCGC
BssSI        3551  CTCGTG               CACGAG(-5/-1)
BstAPI       1946  GCAGGCCATGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstXI         166  CCACTGACATGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I       2096  CCTGAGG              CC^T[ACGT]AGG
Eco47III     1858  AGCGCT               AGC^GCT
Eco81I       2096  CCTGAGG              CC^T[ACGT]AGG
FseI         2592  GGCCGGCC             GGCCGG^CC
HincII       2183  GTTGAC               GT[CT]^[GA]AC
HindII       2183  GTTGAC               GT[CT]^[GA]AC
KspI          844  CCGCGG               CCGC^GG
MamI         1307  GATTGACATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluNI         477  TGGCCA               TGG^CCA
MscI          477  TGGCCA               TGG^CCA
MseI         3772  TTAA                 T^TAA
NcoI          694  CCATGG               C^CATGG
NheI         2083  GCTAGC               G^CTAGC
PaeR7I       1594  CTCGAG               C^TCGAG
PflFI         684  GACCATGTC            GAC[ACGT]^[ACGT][ACGT]GTC
PshAI         929  GACCAACGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SacII         844  CCGCGG               CCGC^GG
ScaI         1204  AGTACT               AGT^ACT
SfiI         2805  GGCCCACTCGGCC        GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SphI         1439  GCATGC               GCATG^C
Tru9I        3772  TTAA                 T^TAA
Tth111I       684  GACCATGTC            GAC[ACGT]^[ACGT][ACGT]GTC
XhoI         1594  CTCGAG               C^TCGAG


###################################
# TWO CUT ENZYMES (    9 enzymes) #
###################################

Alw44I        354  GTGCAC               G^TGCAC
             1832  GTGCAC              
ApaLI         354  GTGCAC               G^TGCAC
             1832  GTGCAC              
BbvCI         950  CCTCAGC              CCTCAGC(-5/-2)
              821  GCTGAGG             
BcgI         1151  CGAGCTGGGTGC         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
             1499  CGACAAGATTGC        
BglI          858  GCCCCTCTGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
             2806  GCCCACTCGGC         
DrdI          522  GACACAGCTGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
             3373  GACGGTGCAGTC        
EcoNI          40  CCTGGCTCAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
             1873  CCTTTGCCAGG         
EcoRI        3254  GAATTC               G^AATTC
             3584  GAATTC              
HgaI          855  GACGC                GACGC(5/10)
             2609  GACGC               


#########################################
# 3 OR MORE CUT ENZYMES (  123 enzymes) #
#########################################

AccII       AciI        AcsI        AcyI        AfaI        
AflIII      AluI        AlwNI       ApaI        ApoI        
Asp700I     AspHI       AvaI        AvaII       BanI        
BanII       BbeI        BbvI        BcnI        BfaI        
BglII       BlpI        BmyI        BpmI        Bpu1102I    
BsaHI       BsaI        BsaJI       BseRI       BsgI        
BsiHKAI     BsiYI       BslI        BsmAI       BsmFI       
BsoBI       Bsp1286I    BspMI       BsrBI       BsrFI       
BsrI        BssKI       BstF5I      BstNI       BstUI       
BstYI       Cac8I       CelII       CfoI        Cfr10I      
Cfr13I      DdeI        DpnII       DraII       DsaI        
EaeI        EarI        EcoO109I    EcoRII      EcoT14I     
FauI        Fnu4HI      FokI        HaeII       HaeIII      
HapII       HhaI        Hin1I       HinP1I      HinfI       
HpaII       HphI        ItaI        KasI        Ksp632I     
MaeI        MaeII       MaeIII      MboI        MboII       
MflI        MnlI        MslI        MspA1I      MspI        
MvaI        MvnI        MwoI        NaeI        NarI        
NciI        NdeII       NgoMIV      NlaIII      NlaIV       
NspI        PflMI       PleI        PpuMI       PstI        
PvuII       RsaI        SacI        Sau3AI      Sau96I      
ScrFI       SfaNI       SfcI        SfoI        SmlI        
StuI        StyI        TaqI        TfiI        TseI        
Tsp45I      Tsp509I     TspRI       TthHB8I     Van91I      
XcmI        XhoII       XmnI