##################################
# NO CUT ENZYMES ( 42 enzymes) #
##################################
AatII Acc65I AclI AhdI AscI
Asp718I AspEI BciVI BsiWI BspDI
BspHI BssSI Bst1107I BstZ17I ClaI
CpoI DraI DrdI Eam1105I EcoRV
EcoT22I FseI KpnI MluI NdeI
NheI NotI NruI NsiI PacI
PmeI Psp1406I PvuI RcaI RsrII
SalI SfiI SnaBI SpeI SspI
SwaI XbaI
###################################
# ONE CUT ENZYMES ( 41 enzymes) #
###################################
AccIII 2358 TCCGGA T^CCGGA
AgeI 3407 ACCGGT A^CCGGT
AseI 4533 ATTAAT AT^TAAT
AsnI 4533 ATTAAT AT^TAAT
Asp700I 1293 GAACCACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvrII 3793 CCTAGG C^CTAGG
BlnI 3793 CCTAGG C^CTAGG
BsaBI 3171 GATGTCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI 1543 GAGACC GGTCTC(1/5)
BseAI 2358 TCCGGA T^CCGGA
BsmI 4769 GCATTC GAATGC(1/-1)
BspEI 2358 TCCGGA T^CCGGA
BspLU11I 2720 ACATGT A^CATGT
BssHII 1863 GCGCGC G^CGCGC
BstAPI 4898 GCAGGCCTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI 136 TTCGAA TT^CGAA
BstEII 2667 GGTGACC G^GT[ACGT]ACC
BstPI 2667 GGTGACC G^GT[ACGT]ACC
DraIII 1396 CACGGAGTG CAC[ACGT][ACGT][ACGT]^GTG
EcoO65I 2667 GGTGACC G^GT[ACGT]ACC
HindIII 4130 AAGCTT A^AGCTT
HpaI 5751 GTTAAC GTT^AAC
KspI 64 CCGCGG CCGC^GG
MamI 3171 GATGTCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 1903 CAATTG C^AATTG
MroI 2358 TCCGGA T^CCGGA
MunI 1903 CAATTG C^AATTG
NaeI 25 GCCGGC GCC^GGC
NgoMIV 25 GCCGGC G^CCGGC
NspV 136 TTCGAA TT^CGAA
PaeR7I 965 CTCGAG C^TCGAG
PinAI 3407 ACCGGT A^CCGGT
PshAI 1995 GACAGGTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI 4533 ATTAAT AT^TAAT
SacII 64 CCGCGG CCGC^GG
SapI 5036 GAAGAGC GCTCTTC(1/4)
ScaI 3188 AGTACT AGT^ACT
SexAI 2483 ACCTGGT A^CC[AT]GGT
SfuI 136 TTCGAA TT^CGAA
XhoI 965 CTCGAG C^TCGAG
XmnI 1293 GAACCACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 34 enzymes) #
###################################
AccI 2488 GTAGAC GT^[AC][GT]AC
4247 GTCTAC
AflIII 1875 ACACGT A^C[GA][CT]GT
2720 ACATGT
AspI 1750 GACACCGTC GAC[ACGT]^[ACGT][ACGT]GTC
2056 GACATGGTC
AviII 569 TGCGCA TGC^GCA
2468 TGCGCA
BamHI 3990 GGATCC G^GATCC
5422 GGATCC
BbeI 350 GGCGCC GGCGC^C
2260 GGCGCC
BbrPI 598 CACGTG CAC^GTG
1876 CACGTG
BbsI 1756 GTCTTC GAAGAC(2/6)
3696 GTCTTC
BbvCI 3267 CCTCAGC CCTCAGC(-5/-2)
4273 CCTCAGC
BcgI 957 GCACCCCACTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
3979 GCAGGGGGCTCG
BclI 917 TGATCA T^GATCA
4432 TGATCA
BglII 1661 AGATCT A^GATCT
2024 AGATCT
BpuAI 1756 GTCTTC GAAGAC(2/6)
3696 GTCTTC
BsiEI 1767 CGGCCG CG[GA][CT]^CG
1792 CGGCCG
BsmBI 112 GAGACG CGTCTC(1/5)
2173 GAGACG
BsrBI 1000 CCGCTC CCGCTC(-3/-3)
2853 CCGCTC
EagI 1767 CGGCCG C^GGCCG
1792 CGGCCG
EclXI 1767 CGGCCG C^GGCCG
1792 CGGCCG
Eco52I 1767 CGGCCG C^GGCCG
1792 CGGCCG
EcoNI 1089 CCTCCTGCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
4707 CCTGGGTTAGG
EcoRI 3165 GAATTC G^AATTC
3439 GAATTC
FbaI 917 TGATCA T^GATCA
4432 TGATCA
FspI 569 TGCGCA TGC^GCA
2468 TGCGCA
KasI 350 GGCGCC G^GCGCC
2260 GGCGCC
NarI 350 GGCGCC GG^CGCC
2260 GGCGCC
PflFI 1750 GACACCGTC GAC[ACGT]^[ACGT][ACGT]GTC
2056 GACATGGTC
PmaCI 598 CACGTG CAC^GTG
1876 CACGTG
PmlI 598 CACGTG CAC^GTG
1876 CACGTG
PpuMI 2480 AGGACCT [GA]G^G[AT]CC[CT]
3955 AGGACCT
SacI 835 GAGCTC GAGCT^C
4422 GAGCTC
SfoI 350 GGCGCC GGC^GCC
2260 GGCGCC
SgrAI 24 CGCCGGCG C[GA]^CCGG[CT]G
3406 CACCGGTG
SphI 1322 GCATGC GCATG^C
1922 GCATGC
Tth111I 1750 GACACCGTC GAC[ACGT]^[ACGT][ACGT]GTC
2056 GACATGGTC
#########################################
# 3 OR MORE CUT ENZYMES ( 141 enzymes) #
#########################################
AccII AciI AcsI AcyI AfaI
AflII AluI Alw44I AlwI AlwNI
AocI Aor51HI ApaI ApaLI ApoI
AspHI AvaI AvaII BalI BanI
BanII BbvI BcnI BfaI BfrI
BglI BlpI BmyI BpmI Bpu1102I
BsaAI BsaHI BsaJI BsaWI BseRI
BsgI BsiHKAI BsiYI BslI BsmAI
BsmFI BsoBI Bsp1286I Bsp1407I BspMI
BsrDI BsrFI BsrGI BsrI BssKI
BstF5I BstNI BstUI BstXI BstYI
Bsu36I Cac8I CelII CfoI Cfr10I
Cfr13I DdeI DpnII DraII DsaI
EaeI EarI Eco47III Eco81I EcoO109I
EcoRII EcoT14I FauI Fnu4HI FokI
HaeII HaeIII HapII HgaI HhaI
Hin1I HinP1I HincII HindII HinfI
HpaII HphI ItaI Ksp632I MaeI
MaeII MaeIII MboI MboII MflI
MluNI MnlI MscI MseI MslI
MspA1I MspI MvaI MvnI MwoI
NciI NcoI NdeII NlaIII NlaIV
NspI PflMI PleI PstI PvuII
RsaI Sau3AI Sau96I SbfI ScrFI
SfaNI SfcI SmaI SmlI Sse8387I
SspBI StuI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I Van91I XcmI XhoII XmaCI
XmaI