##################################
# NO CUT ENZYMES ( 57 enzymes) #
##################################
AgeI Aor51HI ApaI AscI AseI
AsnI BamHI BbrPI BbvCI BcgI
BclI BglI BglII BsaI BsiWI
BspDI BspLU11I BssHII Bst1107I BstZ17I
ClaI CpoI DrdI Eco47III FbaI
FseI MfeI MluI MunI NdeI
NotI NruI PacI PaeR7I PinAI
PmaCI PmeI PmlI PshAI PshBI
PvuI RsrII SalI SbfI SfiI
SgrAI SmaI SnaBI SpeI SphI
Sse8387I StuI SwaI XbaI XhoI
XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 41 enzymes) #
###################################
AatII 345 GACGTC GACGT^C
Acc65I 3882 GGTACC G^GTACC
AccIII 2453 TCCGGA T^CCGGA
AclI 2614 AACGTT AA^CGTT
AflII 3287 CTTAAG C^TTAAG
AhdI 945 GACCTCATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Asp700I 3417 GAATGAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 3882 GGTACC G^GTACC
AspEI 945 GACCTCATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 342 GACGACGTC GAC[ACGT]^[ACGT][ACGT]GTC
AviII 1435 TGCGCA TGC^GCA
BciVI 3765 GTATCC GTATCC(6/5)
BfrI 3287 CTTAAG C^TTAAG
BsaAI 393 TACGTG [CT]AC^GT[GA]
BsaWI 2453 TCCGGA [AT]^CCGG[AT]
BseAI 2453 TCCGGA T^CCGGA
BspEI 2453 TCCGGA T^CCGGA
BspHI 3838 TCATGA T^CATGA
BsrBI 1100 CCGCTC CCGCTC(-3/-3)
BssSI 1353 CTCGTG CACGAG(-5/-1)
BstAPI 251 GCACACGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI 834 TTCGAA TT^CGAA
Eam1105I 945 GACCTCATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoNI 4425 CCTCCTTTAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRI 3421 GAATTC G^AATTC
EcoRV 4557 GATATC GAT^ATC
FspI 1435 TGCGCA TGC^GCA
HincII 3096 GTTAAC GT[CT]^[GA]AC
HindII 3096 GTTAAC GT[CT]^[GA]AC
HpaI 3096 GTTAAC GTT^AAC
KpnI 3882 GGTACC GGTAC^C
MroI 2453 TCCGGA T^CCGGA
NheI 2628 GCTAGC G^CTAGC
NspV 834 TTCGAA TT^CGAA
PflFI 342 GACGACGTC GAC[ACGT]^[ACGT][ACGT]GTC
Psp1406I 2614 AACGTT AA^CGTT
RcaI 3838 TCATGA T^CATGA
SapI 383 GCTCTTC GCTCTTC(1/4)
SfuI 834 TTCGAA TT^CGAA
Tth111I 342 GACGACGTC GAC[ACGT]^[ACGT][ACGT]GTC
XmnI 3417 GAATGAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 31 enzymes) #
###################################
AccI 3516 GTCTAC GT^[AC][GT]AC
4193 GTAGAC
AflIII 178 ACGTGT A^C[GA][CT]GT
4600 ACACGT
Alw44I 2203 GTGCAC G^TGCAC
2832 GTGCAC
ApaLI 2203 GTGCAC G^TGCAC
2832 GTGCAC
BbeI 144 GGCGCC GGCGC^C
1408 GGCGCC
BspMI 4791 ACCTGC ACCTGC(4/8)
3694 GCAGGT
BstEII 3893 GGTTACC G^GT[ACGT]ACC
4204 GGTGACC
BstPI 3893 GGTTACC G^GT[ACGT]ACC
4204 GGTGACC
DraI 1568 TTTAAA TTT^AAA
4942 TTTAAA
DraIII 761 CACCATGTG CAC[ACGT][ACGT][ACGT]^GTG
1552 CACCAAGTG
EagI 89 CGGCCG C^GGCCG
139 CGGCCG
EarI 384 CTCTTC CTCTTC(1/4)
3282 CTCTTC
EclXI 89 CGGCCG C^GGCCG
139 CGGCCG
Eco52I 89 CGGCCG C^GGCCG
139 CGGCCG
EcoO65I 3893 GGTTACC G^GT[ACGT]ACC
4204 GGTGACC
HgaI 275 GACGC GACGC(5/10)
624 GACGC
KasI 144 GGCGCC G^GCGCC
1408 GGCGCC
Ksp632I 384 CTCTTC CTCTTC(1/4)
3282 CTCTTC
KspI 59 CCGCGG CCGC^GG
92 CCGCGG
NaeI 81 GCCGGC GCC^GGC
141 GCCGGC
NarI 144 GGCGCC GG^CGCC
1408 GGCGCC
NcoI 524 CCATGG C^CATGG
3769 CCATGG
NgoMIV 81 GCCGGC G^CCGGC
141 GCCGGC
PflMI 2782 CCATGACATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3269 CCAAAGGTTGG
PstI 509 CTGCAG CTGCA^G
2870 CTGCAG
SacII 59 CCGCGG CCGC^GG
92 CCGCGG
ScaI 472 AGTACT AGT^ACT
1921 AGTACT
SexAI 988 ACCTGGT A^CC[AT]GGT
2795 ACCTGGT
SfoI 144 GGCGCC GGC^GCC
1408 GGCGCC
SspI 2524 AATATT AAT^ATT
4045 AATATT
Van91I 2782 CCATGACATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3269 CCAAAGGTTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 129 enzymes) #
#########################################
AccII AciI AcsI AcyI AfaI
AluI AlwI AlwNI AocI ApoI
AspHI AvaI AvaII AvrII BalI
BanI BanII BbsI BbvI BcnI
BfaI BlnI BlpI BmyI BpmI
Bpu1102I BpuAI BsaBI BsaHI BsaJI
BseRI BsgI BsiEI BsiHKAI BsiYI
BslI BsmAI BsmBI BsmFI BsmI
BsoBI Bsp1286I Bsp1407I BsrDI BsrFI
BsrGI BsrI BssKI BstF5I BstNI
BstUI BstXI BstYI Bsu36I Cac8I
CelII CfoI Cfr10I Cfr13I DdeI
DpnII DraII DsaI EaeI Eco81I
EcoO109I EcoRII EcoT14I EcoT22I FauI
Fnu4HI FokI HaeII HaeIII HapII
HhaI Hin1I HinP1I HindIII HinfI
HpaII HphI ItaI MaeI MaeII
MaeIII MamI MboI MboII MflI
MluNI MnlI MscI MseI MslI
MspA1I MspI MvaI MvnI MwoI
NciI NdeII NlaIII NlaIV NsiI
NspI PleI PpuMI PvuII RsaI
SacI Sau3AI Sau96I ScrFI SfaNI
SfcI SmlI SspBI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I XcmI XhoII