##################################
# NO CUT ENZYMES ( 91 enzymes) #
##################################
AatII AccII AccIII AclI AcyI
AgeI Alw44I AlwNI AocI Aor51HI
ApaI ApaLI AscI AspI AviII
BamHI BbeI BciVI BclI BglI
BlpI Bpu1102I BsaHI BsaWI BseAI
BsiEI BsiWI BspDI BspEI BsrBI
BsrFI BssHII BssSI BstBI BstUI
Bsu36I CelII Cfr10I ClaI DrdI
EagI EclXI Eco47III Eco52I Eco81I
EcoNI EcoRI EcoT22I FauI FbaI
FseI FspI HaeII Hin1I HpaI
KasI KspI MluI MroI MvnI
NaeI NarI NgoMIV NheI NotI
NruI NsiI NspV PacI PflFI
PinAI PmeI PshAI Psp1406I PvuI
SacI SacII SalI SapI SbfI
SexAI SfiI SfoI SfuI SgrAI
SmaI Sse8387I StuI Tth111I XmaCI
XmaI
###################################
# ONE CUT ENZYMES ( 36 enzymes) #
###################################
Acc65I 2496 GGTACC G^GTACC
AflII 1425 CTTAAG C^TTAAG
AseI 3385 ATTAAT AT^TAAT
AsnI 3385 ATTAAT AT^TAAT
Asp718I 2496 GGTACC G^GTACC
AvaI 193 CTCGAG C^[CT]CG[GA]G
AvrII 4383 CCTAGG C^CTAGG
BanII 319 GGGCTC G[GA]GC[CT]^C
BbrPI 687 CACGTG CAC^GTG
BbsI 1120 GAAGAC GAAGAC(2/6)
BfrI 1425 CTTAAG C^TTAAG
BglII 532 AGATCT A^GATCT
BlnI 4383 CCTAGG C^CTAGG
BpuAI 1120 GAAGAC GAAGAC(2/6)
BsaI 272 GAGACC GGTCTC(1/5)
BsgI 2276 GTGCAG GTGCAG(16/14)
BsmI 1663 GAATGC GAATGC(1/-1)
BsoBI 193 CTCGAG C^[CT]CG[GA]G
CfoI 2814 GCGC GCG^C
CpoI 2932 CGGACCG CG^G[AT]CCG
DraIII 3750 CACGATGTG CAC[ACGT][ACGT][ACGT]^GTG
HhaI 2814 GCGC GCG^C
HinP1I 2814 GCGC G^CGC
KpnI 2496 GGTACC GGTAC^C
PaeR7I 193 CTCGAG C^TCGAG
PflMI 3851 CCATCAATTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI 687 CACGTG CAC^GTG
PmlI 687 CACGTG CAC^GTG
PshBI 3385 ATTAAT AT^TAAT
RsrII 2932 CGGACCG CG^G[AT]CCG
ScaI 1308 AGTACT AGT^ACT
SnaBI 2115 TACGTA TAC^GTA
SpeI 1390 ACTAGT A^CTAGT
SphI 721 GCATGC GCATG^C
Van91I 3851 CCATCAATTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoI 193 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 26 enzymes) #
###################################
AhdI 2934 GACCGCCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
4877 GACTATTAGTC
AspEI 2934 GACCGCCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
4877 GACTATTAGTC
AspHI 190 GTGCTC G[AT]GC[AT]^C
4579 GAGCAC
BbvCI 2809 CCTCAGC CCTCAGC(-5/-2)
343 GCTGAGG
BcgI 1332 CGATCAGGCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
1271 GCAATGTGATCG
BsaAI 687 CACGTG [CT]AC^GT[GA]
2115 TACGTA
BseRI 2778 GAGGAG GAGGAG(10/8)
2967 GAGGAG
BsiHKAI 190 GTGCTC G[AT]GC[AT]^C
4579 GAGCAC
BsmBI 277 CGTCTC CGTCTC(1/5)
2186 GAGACG
Bsp1407I 4540 TGTACA T^GTACA
4821 TGTACA
BspHI 2892 TCATGA T^CATGA
4180 TCATGA
BspLU11I 497 ACATGT A^CATGT
4076 ACATGT
BsrGI 4540 TGTACA T^GTACA
4821 TGTACA
Cac8I 721 GCATGC GC[ACGT]^[ACGT]GC
787 GCTGGC
Eam1105I 2934 GACCGCCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
4877 GACTATTAGTC
EarI 4467 CTCTTC CTCTTC(1/4)
2848 GAAGAG
HindIII 1376 AAGCTT A^AGCTT
3842 AAGCTT
Ksp632I 4467 CTCTTC CTCTTC(1/4)
2848 GAAGAG
NcoI 668 CCATGG C^CATGG
1206 CCATGG
PpuMI 9 GGGTCCC [GA]G^G[AT]CC[CT]
1179 GGGTCCC
PvuII 1572 CAGCTG CAG^CTG
2072 CAGCTG
RcaI 2892 TCATGA T^CATGA
4180 TCATGA
SspBI 4540 TGTACA T^GTACA
4821 TGTACA
SspI 3245 AATATT AAT^ATT
4445 AATATT
SwaI 1133 ATTTAAAT ATTT^AAAT
3230 ATTTAAAT
XbaI 213 TCTAGA T^CTAGA
4042 TCTAGA
#########################################
# 3 OR MORE CUT ENZYMES ( 105 enzymes) #
#########################################
AccI AciI AcsI AfaI AflIII
AluI AlwI ApoI Asp700I AvaII
BalI BanI BbvI BcnI BfaI
BmyI BpmI BsaBI BsaJI BsiYI
BslI BsmAI BsmFI Bsp1286I BspMI
BsrDI BsrI BssKI Bst1107I BstAPI
BstEII BstF5I BstNI BstPI BstXI
BstYI BstZ17I Cfr13I DdeI DpnII
DraI DraII DsaI EaeI EcoO109I
EcoO65I EcoRII EcoRV EcoT14I Fnu4HI
FokI HaeIII HapII HgaI HincII
HindII HinfI HpaII HphI ItaI
MaeI MaeII MaeIII MamI MboI
MboII MfeI MflI MluNI MnlI
MscI MseI MslI MspA1I MspI
MunI MvaI MwoI NciI NdeI
NdeII NlaIII NlaIV NspI PleI
PstI RsaI Sau3AI Sau96I ScrFI
SfaNI SfcI SmlI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I XcmI XhoII XmnI