##################################
# NO CUT ENZYMES ( 90 enzymes) #
##################################
Acc65I AccIII AclI AflII AgeI
Alw44I AocI Aor51HI ApaLI AscI
Asp718I AvaI AviII AvrII BamHI
BbrPI BbvCI BfrI BlnI BlpI
Bpu1102I BsaAI BsaBI BsaWI BseAI
BsiWI BsmBI BsoBI BspDI BspEI
BsrBI BsrDI BsrFI BstBI Bsu36I
CelII Cfr10I ClaI CpoI DraIII
DsaI EagI EclXI Eco47III Eco52I
Eco81I EcoT22I FseI FspI KpnI
KspI MamI MfeI MluI MroI
MunI NaeI NcoI NdeI NgoMIV
NheI NotI NruI NsiI NspV
PacI PaeR7I PinAI PmaCI PmeI
PmlI Psp1406I PvuI RsrII SacI
SacII SalI SbfI ScaI SfiI
SfuI SgrAI SmaI SnaBI SpeI
Sse8387I StuI XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 39 enzymes) #
###################################
AatII 3 GACGTC GACGT^C
AflIII 2391 ACATGT A^C[GA][CT]GT
AhdI 1695 GACTGCAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
ApaI 1894 GGGCCC GGGCC^C
Asp700I 2268 GAAAGGATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI 1695 GACTGCAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspHI 633 GAGCAC G[AT]GC[AT]^C
AspI 3685 GACAGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
BbeI 265 GGCGCC GGCGC^C
BciVI 4218 GTATCC GTATCC(6/5)
BsaI 3847 GGTCTC GGTCTC(1/5)
BsiEI 185 CGGTCG CG[GA][CT]^CG
BsiHKAI 633 GAGCAC G[AT]GC[AT]^C
BsmI 4232 GAATGC GAATGC(1/-1)
BspLU11I 2391 ACATGT A^CATGT
BssHII 712 GCGCGC G^CGCGC
BssSI 3761 CTCGTG CACGAG(-5/-1)
BstEII 279 GGTTACC G^GT[ACGT]ACC
BstPI 279 GGTTACC G^GT[ACGT]ACC
DrdI 367 GACCTTGATGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eam1105I 1695 GACTGCAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoNI 4222 CCTTGTTGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 279 GGTTACC G^GT[ACGT]ACC
EcoRI 3143 GAATTC G^AATTC
HpaI 874 GTTAAC GTT^AAC
KasI 265 GGCGCC G^GCGCC
NarI 265 GGCGCC GG^CGCC
PflFI 3685 GACAGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PshAI 2678 GACAAGTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PvuII 4765 CAGCTG CAG^CTG
SapI 934 GCTCTTC GCTCTTC(1/4)
SexAI 1573 ACCTGGT A^CC[AT]GGT
SfoI 265 GGCGCC GGC^GCC
SphI 1158 GCATGC GCATG^C
SwaI 2867 ATTTAAAT ATTT^AAAT
Tth111I 3685 GACAGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
XbaI 4263 TCTAGA T^CTAGA
XcmI 836 CCAAAATCTTACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmnI 2268 GAAAGGATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 26 enzymes) #
###################################
AcyI 3 GACGTC G[GA]^CG[CT]C
265 GGCGCC
AseI 2992 ATTAAT AT^TAAT
4987 ATTAAT
AsnI 2992 ATTAAT AT^TAAT
4987 ATTAAT
BalI 833 TGGCCA TGG^CCA
1004 TGGCCA
BanII 1894 GGGCCC G[GA]GC[CT]^C
4895 GAGCCC
BcgI 456 CGAAGGGTGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
3190 GCAAAATATTCG
BclI 1881 TGATCA T^GATCA
3727 TGATCA
BglI 1944 GCCAACAAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
3751 GCCTCTCAGGC
BglII 344 AGATCT A^GATCT
1876 AGATCT
BsaHI 3 GACGTC G[GA]^CG[CT]C
265 GGCGCC
Bsp1407I 1810 TGTACA T^GTACA
4797 TGTACA
BsrGI 1810 TGTACA T^GTACA
4797 TGTACA
Bst1107I 4100 GTATAC GTA^TAC
4844 GTATAC
BstAPI 1428 GCACCAAATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
2529 GCAAGCCATGC
BstZ17I 4100 GTATAC GTA^TAC
4844 GTATAC
EcoRV 1548 GATATC GAT^ATC
4068 GATATC
FbaI 1881 TGATCA T^GATCA
3727 TGATCA
HgaI 641 GCGTC GACGC(5/10)
1901 GCGTC
Hin1I 3 GACGTC G[GA]^CG[CT]C
265 GGCGCC
MluNI 833 TGGCCA TGG^CCA
1004 TGGCCA
MscI 833 TGGCCA TGG^CCA
1004 TGGCCA
MspA1I 895 CCGCTG C[AC]G^C[GT]G
4765 CAGCTG
PshBI 2992 ATTAAT AT^TAAT
4987 ATTAAT
SspBI 1810 TGTACA T^GTACA
4797 TGTACA
TaqI 1299 TCGA T^CGA
3199 TCGA
TthHB8I 1299 TCGA T^CGA
3199 TCGA
#########################################
# 3 OR MORE CUT ENZYMES ( 103 enzymes) #
#########################################
AccI AccII AciI AcsI AfaI
AluI AlwI AlwNI ApoI AvaII
BanI BbsI BbvI BcnI BfaI
BmyI BpmI BpuAI BsaJI BseRI
BsgI BsiYI BslI BsmAI BsmFI
Bsp1286I BspHI BspMI BsrI BssKI
BstF5I BstNI BstUI BstXI BstYI
Cac8I CfoI Cfr13I DdeI DpnII
DraI DraII EaeI EarI EcoO109I
EcoRII EcoT14I FauI Fnu4HI FokI
HaeII HaeIII HapII HhaI HinP1I
HincII HindII HindIII HinfI HpaII
HphI ItaI Ksp632I MaeI MaeII
MaeIII MboI MboII MflI MnlI
MseI MslI MspI MvaI MvnI
MwoI NciI NdeII NlaIII NlaIV
NspI PflMI PleI PpuMI PstI
RcaI RsaI Sau3AI Sau96I ScrFI
SfaNI SfcI SmlI SspI StyI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI Van91I XhoII