##################################
# NO CUT ENZYMES ( 72 enzymes) #
##################################
AatII Acc65I AccIII AclI AflII
Alw44I ApaLI AscI AseI AsnI
Asp718I AviII AvrII BbrPI BbvCI
BcgI BcnI BfrI BlnI BsaBI
BseAI BsiEI BsiWI BspDI BspEI
BspHI BsrBI BssHII Bst1107I BstAPI
BstZ17I ClaI CpoI DraIII EagI
EclXI Eco52I FseI FspI HpaI
KpnI KspI MamI MroI NaeI
NciI NdeI NgoMIV NheI NotI
NruI PacI PaeR7I PmaCI PmeI
PmlI PshAI PshBI Psp1406I PvuI
RcaI RsrII SacII SalI SfiI
SgrAI SmaI SnaBI SwaI XhoI
XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 39 enzymes) #
###################################
AccI 1456 GTCTAC GT^[AC][GT]AC
AcyI 202 GGCGCC G[GA]^CG[CT]C
AgeI 3874 ACCGGT A^CCGGT
AocI 3023 CCTGAGG CC^T[ACGT]AGG
Aor51HI 3331 AGCGCT AGC^GCT
ApaI 308 GGGCCC GGGCC^C
AspI 2765 GACCATGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 918 CCCGAG C^[CT]CG[GA]G
BamHI 412 GGATCC G^GATCC
BbeI 202 GGCGCC GGCGC^C
BsaHI 202 GGCGCC G[GA]^CG[CT]C
BsmBI 361 GAGACG CGTCTC(1/5)
BsoBI 918 CCCGAG C^[CT]CG[GA]G
BsrFI 3874 ACCGGT [GA]^CCGG[CT]
BssSI 690 CTCGTG CACGAG(-5/-1)
BstBI 3291 TTCGAA TT^CGAA
BstXI 1401 CCATCCCTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 3023 CCTGAGG CC^T[ACGT]AGG
Cfr10I 3874 ACCGGT [GA]^CCGG[CT]
DraI 4618 TTTAAA TTT^AAA
Eco47III 3331 AGCGCT AGC^GCT
Eco81I 3023 CCTGAGG CC^T[ACGT]AGG
Hin1I 202 GGCGCC G[GA]^CG[CT]C
KasI 202 GGCGCC G^GCGCC
MfeI 2258 CAATTG C^AATTG
MluI 188 ACGCGT A^CGCGT
MunI 2258 CAATTG C^AATTG
NarI 202 GGCGCC GG^CGCC
NspV 3291 TTCGAA TT^CGAA
PflFI 2765 GACCATGTC GAC[ACGT]^[ACGT][ACGT]GTC
PinAI 3874 ACCGGT A^CCGGT
SacI 284 GAGCTC GAGCT^C
SbfI 680 CCTGCAGG CCTGCA^GG
SexAI 4310 ACCTGGT A^CC[AT]GGT
SfoI 202 GGCGCC GGC^GCC
SfuI 3291 TTCGAA TT^CGAA
SpeI 5155 ACTAGT A^CTAGT
Sse8387I 680 CCTGCAGG CCTGCA^GG
Tth111I 2765 GACCATGTC GAC[ACGT]^[ACGT][ACGT]GTC
###################################
# TWO CUT ENZYMES ( 28 enzymes) #
###################################
AhdI 668 GACATGGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
5115 GACTTCTTGTC
AspEI 668 GACATGGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
5115 GACTTCTTGTC
BanI 202 GGCGCC G^G[CT][GA]CC
2011 GGCACC
BclI 603 TGATCA T^GATCA
1126 TGATCA
BglI 2003 GCCTGTGAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
4324 GCCATTTGGGC
BglII 3140 AGATCT A^GATCT
4903 AGATCT
BlpI 1444 GCTCAGC GC^T[ACGT]AGC
5021 GCTAAGC
Bpu1102I 1444 GCTCAGC GC^T[ACGT]AGC
5021 GCTAAGC
BsaAI 805 CACGTA [CT]AC^GT[GA]
1459 TACGTG
BsaI 3411 GGTCTC GGTCTC(1/5)
3579 GGTCTC
Bsp1407I 4264 TGTACA T^GTACA
5494 TGTACA
BsrGI 4264 TGTACA T^GTACA
5494 TGTACA
BstEII 1198 GGTAACC G^GT[ACGT]ACC
2860 GGTGACC
BstPI 1198 GGTAACC G^GT[ACGT]ACC
2860 GGTGACC
CelII 1444 GCTCAGC GC^T[ACGT]AGC
5021 GCTAAGC
DrdI 586 GACCTTCCAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2263 GACCAGCTGGTC
Eam1105I 668 GACATGGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
5115 GACTTCTTGTC
EcoO65I 1198 GGTAACC G^GT[ACGT]ACC
2860 GGTGACC
EcoRI 1557 GAATTC G^AATTC
3692 GAATTC
EcoT22I 2920 ATGCAT ATGCA^T
4999 ATGCAT
FbaI 603 TGATCA T^GATCA
1126 TGATCA
HindIII 556 AAGCTT A^AGCTT
575 AAGCTT
NsiI 2920 ATGCAT ATGCA^T
4999 ATGCAT
SapI 1241 GAAGAGC GCTCTTC(1/4)
3993 GCTCTTC
ScaI 60 AGTACT AGT^ACT
5152 AGTACT
SspBI 4264 TGTACA T^GTACA
5494 TGTACA
SspI 480 AATATT AAT^ATT
3321 AATATT
XbaI 2582 TCTAGA T^CTAGA
2999 TCTAGA
#########################################
# 3 OR MORE CUT ENZYMES ( 119 enzymes) #
#########################################
AccII AciI AcsI AfaI AflIII
AluI AlwI AlwNI ApoI Asp700I
AspHI AvaII BalI BanII BbsI
BbvI BciVI BfaI BmyI BpmI
BpuAI BsaJI BsaWI BseRI BsgI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsmI Bsp1286I BspLU11I BspMI BsrDI
BsrI BssKI BstF5I BstNI BstUI
BstYI Cac8I CfoI Cfr13I DdeI
DpnII DraII DsaI EaeI EarI
EcoNI EcoO109I EcoRII EcoRV EcoT14I
FauI Fnu4HI FokI HaeII HaeIII
HapII HgaI HhaI HinP1I HincII
HindII HinfI HpaII HphI ItaI
Ksp632I MaeI MaeII MaeIII MboI
MboII MflI MluNI MnlI MscI
MseI MslI MspA1I MspI MvaI
MvnI MwoI NcoI NdeII NlaIII
NlaIV NspI PflMI PleI PpuMI
PstI PvuII RsaI Sau3AI Sau96I
ScrFI SfaNI SfcI SmlI SphI
StuI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
Van91I XcmI XhoII XmnI