################################## # NO CUT ENZYMES ( 59 enzymes) # ################################## Acc65I AccI AclI AflII AgeI AscI AseI AsnI Asp700I Asp718I AviII AvrII BciVI BclI BfrI BlnI BsiWI BsmI BspDI BspHI Bst1107I BstBI BstZ17I ClaI CpoI DrdI EcoRI EcoRV FbaI FseI FspI HincII HindII HpaI KpnI MfeI MluI MunI NdeI NheI NotI NruI NspV PacI PinAI PmeI PshAI PshBI Psp1406I RcaI RsrII SacI SalI ScaI SfuI SpeI SphI XbaI XmnI ################################### # ONE CUT ENZYMES ( 35 enzymes) # ################################### AatII 1272 GACGTC GACGT^C AcsI 5402 AAATTT [GA]^AATT[CT] AocI 3435 CCTGAGG CC^T[ACGT]AGG ApoI 5402 AAATTT [GA]^AATT[CT] BbrPI 2670 CACGTG CAC^GTG BbvCI 3905 GCTGAGG CCTCAGC(-5/-2) BglII 349 AGATCT A^GATCT BsaBI 350 GATCTCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC Bsp1407I 5383 TGTACA T^GTACA BspLU11I 1150 ACATGT A^CATGT BsrGI 5383 TGTACA T^GTACA BstEII 1119 GGTGACC G^GT[ACGT]ACC BstPI 1119 GGTGACC G^GT[ACGT]ACC Bsu36I 3435 CCTGAGG CC^T[ACGT]AGG DraI 5399 TTTAAA TTT^AAA EagI 4055 CGGCCG C^GGCCG EclXI 4055 CGGCCG C^GGCCG Eco52I 4055 CGGCCG C^GGCCG Eco81I 3435 CCTGAGG CC^T[ACGT]AGG EcoNI 1478 CCTCGTGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoO65I 1119 GGTGACC G^GT[ACGT]ACC HindIII 1814 AAGCTT A^AGCTT KspI 5100 CCGCGG CCGC^GG MamI 350 GATCTCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC PaeR7I 912 CTCGAG C^TCGAG PmaCI 2670 CACGTG CAC^GTG PmlI 2670 CACGTG CAC^GTG PvuI 36 CGATCG CGAT^CG SacII 5100 CCGCGG CCGC^GG SnaBI 2274 TACGTA TAC^GTA SspBI 5383 TGTACA T^GTACA SspI 5395 AATATT AAT^ATT SwaI 5398 ATTTAAAT ATTT^AAAT Tsp509I 5403 AATT ^AATT XhoI 912 CTCGAG C^TCGAG ################################### # TWO CUT ENZYMES ( 33 enzymes) # ################################### AccIII 2365 TCCGGA T^CCGGA 2993 TCCGGA AhdI 1755 GACGTGGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 4826 GACATCACGTC Aor51HI 406 AGCGCT AGC^GCT 871 AGCGCT AspEI 1755 GACGTGGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 4826 GACATCACGTC AspI 480 GACCTGGTC GAC[ACGT]^[ACGT][ACGT]GTC 4343 GACACTGTC BamHI 2851 GGATCC G^GATCC 3153 GGATCC BcgI 4660 CGACCTCACTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) 1471 GCAAGATCCTCG BlpI 188 GCTCAGC GC^T[ACGT]AGC 1073 GCTGAGC Bpu1102I 188 GCTCAGC GC^T[ACGT]AGC 1073 GCTGAGC BsaAI 2274 TACGTA [CT]AC^GT[GA] 2670 CACGTG BsaWI 2365 TCCGGA [AT]^CCGG[AT] 2993 TCCGGA BseAI 2365 TCCGGA T^CCGGA 2993 TCCGGA BsiEI 36 CGATCG CG[GA][CT]^CG 4055 CGGCCG BsmBI 1700 CGTCTC CGTCTC(1/5) 4768 GAGACG BspEI 2365 TCCGGA T^CCGGA 2993 TCCGGA BsrDI 4930 CATTGC GCAATG(2/0) 1792 GCAATG BssHII 53 GCGCGC G^CGCGC 507 GCGCGC CelII 188 GCTCAGC GC^T[ACGT]AGC 1073 GCTGAGC DraIII 1044 CACCATGTG CAC[ACGT][ACGT][ACGT]^GTG 3670 CACCGAGTG Eam1105I 1755 GACGTGGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 4826 GACATCACGTC Eco47III 406 AGCGCT AGC^GCT 871 AGCGCT EcoT22I 1594 ATGCAT ATGCA^T 3134 ATGCAT MroI 2365 TCCGGA T^CCGGA 2993 TCCGGA MseI 5400 TTAA T^TAA 5431 TTAA NcoI 160 CCATGG C^CATGG 1235 CCATGG NsiI 1594 ATGCAT ATGCA^T 3134 ATGCAT PflFI 480 GACCTGGTC GAC[ACGT]^[ACGT][ACGT]GTC 4343 GACACTGTC PflMI 3920 CCAGGCCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 4717 CCACAGCCTGG SfiI 2757 GGCCTAAAAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC 4942 GGCCCGCCAGGCC SgrAI 3161 CGCCGGCG C[GA]^CCGG[CT]G 4075 CGCCGGCG Tru9I 5400 TTAA T^TAA 5431 TTAA Tth111I 480 GACCTGGTC GAC[ACGT]^[ACGT][ACGT]GTC 4343 GACACTGTC Van91I 3920 CCAGGCCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 4717 CCACAGCCTGG ######################################### # 3 OR MORE CUT ENZYMES ( 131 enzymes) # ######################################### AccII AciI AcyI AfaI AflIII AluI Alw44I AlwI AlwNI ApaI ApaLI AspHI AvaI AvaII BalI BanI BanII BbeI BbsI BbvI BcnI BfaI BglI BmyI BpmI BpuAI BsaHI BsaI BsaJI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I BspMI BsrBI BsrFI BsrI BssKI BssSI BstAPI BstF5I BstNI BstUI BstXI BstYI Cac8I CfoI Cfr10I Cfr13I DdeI DpnII DraII DsaI EaeI EarI EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeII HaeIII HapII HgaI HhaI Hin1I HinP1I HinfI HpaII HphI ItaI KasI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MluNI MnlI MscI MslI MspA1I MspI MvaI MvnI MwoI NaeI NarI NciI NdeII NgoMIV NlaIII NlaIV NspI PleI PpuMI PstI PvuII RsaI SapI Sau3AI Sau96I SbfI ScrFI SexAI SfaNI SfcI SfoI SmaI SmlI Sse8387I StuI StyI TaqI TfiI TseI Tsp45I TspRI TthHB8I XcmI XhoII XmaCI XmaI