##################################
# NO CUT ENZYMES (   59 enzymes) #
##################################

Acc65I      AccI        AclI        AflII       AgeI        
AscI        AseI        AsnI        Asp700I     Asp718I     
AviII       AvrII       BciVI       BclI        BfrI        
BlnI        BsiWI       BsmI        BspDI       BspHI       
Bst1107I    BstBI       BstZ17I     ClaI        CpoI        
DrdI        EcoRI       EcoRV       FbaI        FseI        
FspI        HincII      HindII      HpaI        KpnI        
MfeI        MluI        MunI        NdeI        NheI        
NotI        NruI        NspV        PacI        PinAI       
PmeI        PshAI       PshBI       Psp1406I    RcaI        
RsrII       SacI        SalI        ScaI        SfuI        
SpeI        SphI        XbaI        XmnI        


###################################
# ONE CUT ENZYMES (   35 enzymes) #
###################################

AatII        1272  GACGTC               GACGT^C
AcsI         5402  AAATTT               [GA]^AATT[CT]
AocI         3435  CCTGAGG              CC^T[ACGT]AGG
ApoI         5402  AAATTT               [GA]^AATT[CT]
BbrPI        2670  CACGTG               CAC^GTG
BbvCI        3905  GCTGAGG              CCTCAGC(-5/-2)
BglII         349  AGATCT               A^GATCT
BsaBI         350  GATCTCCATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
Bsp1407I     5383  TGTACA               T^GTACA
BspLU11I     1150  ACATGT               A^CATGT
BsrGI        5383  TGTACA               T^GTACA
BstEII       1119  GGTGACC              G^GT[ACGT]ACC
BstPI        1119  GGTGACC              G^GT[ACGT]ACC
Bsu36I       3435  CCTGAGG              CC^T[ACGT]AGG
DraI         5399  TTTAAA               TTT^AAA
EagI         4055  CGGCCG               C^GGCCG
EclXI        4055  CGGCCG               C^GGCCG
Eco52I       4055  CGGCCG               C^GGCCG
Eco81I       3435  CCTGAGG              CC^T[ACGT]AGG
EcoNI        1478  CCTCGTGAAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I      1119  GGTGACC              G^GT[ACGT]ACC
HindIII      1814  AAGCTT               A^AGCTT
KspI         5100  CCGCGG               CCGC^GG
MamI          350  GATCTCCATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
PaeR7I        912  CTCGAG               C^TCGAG
PmaCI        2670  CACGTG               CAC^GTG
PmlI         2670  CACGTG               CAC^GTG
PvuI           36  CGATCG               CGAT^CG
SacII        5100  CCGCGG               CCGC^GG
SnaBI        2274  TACGTA               TAC^GTA
SspBI        5383  TGTACA               T^GTACA
SspI         5395  AATATT               AAT^ATT
SwaI         5398  ATTTAAAT             ATTT^AAAT
Tsp509I      5403  AATT                 ^AATT
XhoI          912  CTCGAG               C^TCGAG


###################################
# TWO CUT ENZYMES (   33 enzymes) #
###################################

AccIII       2365  TCCGGA               T^CCGGA
             2993  TCCGGA              
AhdI         1755  GACGTGGAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
             4826  GACATCACGTC         
Aor51HI       406  AGCGCT               AGC^GCT
              871  AGCGCT              
AspEI        1755  GACGTGGAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
             4826  GACATCACGTC         
AspI          480  GACCTGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
             4343  GACACTGTC           
BamHI        2851  GGATCC               G^GATCC
             3153  GGATCC              
BcgI         4660  CGACCTCACTGC         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
             1471  GCAAGATCCTCG        
BlpI          188  GCTCAGC              GC^T[ACGT]AGC
             1073  GCTGAGC             
Bpu1102I      188  GCTCAGC              GC^T[ACGT]AGC
             1073  GCTGAGC             
BsaAI        2274  TACGTA               [CT]AC^GT[GA]
             2670  CACGTG              
BsaWI        2365  TCCGGA               [AT]^CCGG[AT]
             2993  TCCGGA              
BseAI        2365  TCCGGA               T^CCGGA
             2993  TCCGGA              
BsiEI          36  CGATCG               CG[GA][CT]^CG
             4055  CGGCCG              
BsmBI        1700  CGTCTC               CGTCTC(1/5)
             4768  GAGACG              
BspEI        2365  TCCGGA               T^CCGGA
             2993  TCCGGA              
BsrDI        4930  CATTGC               GCAATG(2/0)
             1792  GCAATG              
BssHII         53  GCGCGC               G^CGCGC
              507  GCGCGC              
CelII         188  GCTCAGC              GC^T[ACGT]AGC
             1073  GCTGAGC             
DraIII       1044  CACCATGTG            CAC[ACGT][ACGT][ACGT]^GTG
             3670  CACCGAGTG           
Eam1105I     1755  GACGTGGAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
             4826  GACATCACGTC         
Eco47III      406  AGCGCT               AGC^GCT
              871  AGCGCT              
EcoT22I      1594  ATGCAT               ATGCA^T
             3134  ATGCAT              
MroI         2365  TCCGGA               T^CCGGA
             2993  TCCGGA              
MseI         5400  TTAA                 T^TAA
             5431  TTAA                
NcoI          160  CCATGG               C^CATGG
             1235  CCATGG              
NsiI         1594  ATGCAT               ATGCA^T
             3134  ATGCAT              
PflFI         480  GACCTGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
             4343  GACACTGTC           
PflMI        3920  CCAGGCCCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             4717  CCACAGCCTGG         
SfiI         2757  GGCCTAAAAGGCC        GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
             4942  GGCCCGCCAGGCC       
SgrAI        3161  CGCCGGCG             C[GA]^CCGG[CT]G
             4075  CGCCGGCG            
Tru9I        5400  TTAA                 T^TAA
             5431  TTAA                
Tth111I       480  GACCTGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
             4343  GACACTGTC           
Van91I       3920  CCAGGCCCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             4717  CCACAGCCTGG         


#########################################
# 3 OR MORE CUT ENZYMES (  131 enzymes) #
#########################################

AccII       AciI        AcyI        AfaI        AflIII      
AluI        Alw44I      AlwI        AlwNI       ApaI        
ApaLI       AspHI       AvaI        AvaII       BalI        
BanI        BanII       BbeI        BbsI        BbvI        
BcnI        BfaI        BglI        BmyI        BpmI        
BpuAI       BsaHI       BsaI        BsaJI       BseRI       
BsgI        BsiHKAI     BsiYI       BslI        BsmAI       
BsmFI       BsoBI       Bsp1286I    BspMI       BsrBI       
BsrFI       BsrI        BssKI       BssSI       BstAPI      
BstF5I      BstNI       BstUI       BstXI       BstYI       
Cac8I       CfoI        Cfr10I      Cfr13I      DdeI        
DpnII       DraII       DsaI        EaeI        EarI        
EcoO109I    EcoRII      EcoT14I     FauI        Fnu4HI      
FokI        HaeII       HaeIII      HapII       HgaI        
HhaI        Hin1I       HinP1I      HinfI       HpaII       
HphI        ItaI        KasI        Ksp632I     MaeI        
MaeII       MaeIII      MboI        MboII       MflI        
MluNI       MnlI        MscI        MslI        MspA1I      
MspI        MvaI        MvnI        MwoI        NaeI        
NarI        NciI        NdeII       NgoMIV      NlaIII      
NlaIV       NspI        PleI        PpuMI       PstI        
PvuII       RsaI        SapI        Sau3AI      Sau96I      
SbfI        ScrFI       SexAI       SfaNI       SfcI        
SfoI        SmaI        SmlI        Sse8387I    StuI        
StyI        TaqI        TfiI        TseI        Tsp45I      
TspRI       TthHB8I     XcmI        XhoII       XmaCI       
XmaI