##################################
# NO CUT ENZYMES ( 62 enzymes) #
##################################
AatII Acc65I AccIII AclI AgeI
AhdI Aor51HI AscI Asp718I AspEI
AspI BclI BglII BsaBI BsaWI
BseAI BsiWI BspDI BspEI BssHII
Bst1107I BstBI BstEII BstPI BstZ17I
ClaI CpoI DraI DrdI Eam1105I
Eco47III EcoO65I EcoT22I FbaI HpaI
KpnI KspI MamI MroI NotI
NruI NsiI NspV PacI PflFI
PinAI PmeI Psp1406I PvuI RsrII
SacII SalI ScaI SexAI SfiI
SfuI SgrAI SnaBI StuI SwaI
Tth111I XbaI
###################################
# ONE CUT ENZYMES ( 53 enzymes) #
###################################
AccI 188 GTCTAC GT^[AC][GT]AC
AcyI 4191 GGCGCC G[GA]^CG[CT]C
AflII 2395 CTTAAG C^TTAAG
AocI 3619 CCTCAGG CC^T[ACGT]AGG
AseI 1649 ATTAAT AT^TAAT
AsnI 1649 ATTAAT AT^TAAT
BamHI 700 GGATCC G^GATCC
BbeI 4191 GGCGCC GGCGC^C
BbsI 1537 GAAGAC GAAGAC(2/6)
BcgI 4006 GCAGCTTGTTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfrI 2395 CTTAAG C^TTAAG
BpuAI 1537 GAAGAC GAAGAC(2/6)
BsaHI 4191 GGCGCC G[GA]^CG[CT]C
BsiEI 1634 CGGCCG CG[GA][CT]^CG
BsmBI 2065 GAGACG CGTCTC(1/5)
Bsp1407I 2356 TGTACA T^GTACA
BspHI 5360 TCATGA T^CATGA
BsrFI 56 GCCGGC [GA]^CCGG[CT]
BsrGI 2356 TGTACA T^GTACA
Bsu36I 3619 CCTCAGG CC^T[ACGT]AGG
Cfr10I 56 GCCGGC [GA]^CCGG[CT]
EagI 1634 CGGCCG C^GGCCG
EclXI 1634 CGGCCG C^GGCCG
Eco52I 1634 CGGCCG C^GGCCG
Eco81I 3619 CCTCAGG CC^T[ACGT]AGG
EcoRI 199 GAATTC G^AATTC
EcoRV 618 GATATC GAT^ATC
FseI 55 GGCCGGCC GGCCGG^CC
Hin1I 4191 GGCGCC G[GA]^CG[CT]C
HindIII 1548 AAGCTT A^AGCTT
KasI 4191 GGCGCC G^GCGCC
MfeI 3301 CAATTG C^AATTG
MluI 4720 ACGCGT A^CGCGT
MunI 3301 CAATTG C^AATTG
NaeI 56 GCCGGC GCC^GGC
NarI 4191 GGCGCC GG^CGCC
NdeI 818 CATATG CA^TATG
NgoMIV 56 GCCGGC G^CCGGC
NheI 980 GCTAGC G^CTAGC
PaeR7I 4796 CTCGAG C^TCGAG
PshAI 3925 GACAGGAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI 1649 ATTAAT AT^TAAT
RcaI 5360 TCATGA T^CATGA
SapI 2989 GCTCTTC GCTCTTC(1/4)
SfoI 4191 GGCGCC GGC^GCC
SmaI 2958 CCCGGG CCC^GGG
SpeI 1205 ACTAGT A^CTAGT
SphI 337 GCATGC GCATG^C
SspBI 2356 TGTACA T^GTACA
SspI 1077 AATATT AAT^ATT
XhoI 4796 CTCGAG C^TCGAG
XmaCI 2958 CCCGGG C^CCGGG
XmaI 2958 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 27 enzymes) #
###################################
AccII 76 CGCG CG^CG
4721 CGCG
Asp700I 3770 GAAATGATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
4820 GAAACATTTC
AviII 4348 TGCGCA TGC^GCA
4453 TGCGCA
AvrII 2951 CCTAGG C^CTAGG
4087 CCTAGG
BalI 2896 TGGCCA TGG^CCA
3042 TGGCCA
BciVI 535 GGATAC GTATCC(6/5)
868 GGATAC
BglI 1661 GCCATTAGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
4028 GCCAAGCAGGC
BlnI 2951 CCTAGG C^CTAGG
4087 CCTAGG
BspLU11I 1086 ACATGT A^CATGT
2943 ACATGT
BsrBI 2318 CCGCTC CCGCTC(-3/-3)
4254 CCGCTC
BsrDI 2070 GCAATG GCAATG(2/0)
4170 GCAATG
BstUI 76 CGCG CG^CG
4721 CGCG
BstXI 774 CCAGAGCCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
4558 CCAGGAAAATGG
BstYI 700 GGATCC [GA]^GATC[CT]
1764 AGATCC
FspI 4348 TGCGCA TGC^GCA
4453 TGCGCA
HincII 3981 GTTGAC GT[CT]^[GA]AC
4101 GTTGAC
HindII 3981 GTTGAC GT[CT]^[GA]AC
4101 GTTGAC
MflI 700 GGATCC [GA]^GATC[CT]
1764 AGATCC
MluNI 2896 TGGCCA TGG^CCA
3042 TGGCCA
MscI 2896 TGGCCA TGG^CCA
3042 TGGCCA
MvnI 76 CGCG CG^CG
4721 CGCG
SacI 3118 GAGCTC GAGCT^C
3381 GAGCTC
SbfI 42 CCTGCAGG CCTGCA^GG
4883 CCTGCAGG
SmlI 2395 CTTAAG C^T[CT][GA]AG
4796 CTCGAG
Sse8387I 42 CCTGCAGG CCTGCA^GG
4883 CCTGCAGG
XhoII 700 GGATCC [GA]^GATC[CT]
1764 AGATCC
XmnI 3770 GAAATGATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
4820 GAAACATTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 116 enzymes) #
#########################################
AciI AcsI AfaI AflIII AluI
Alw44I AlwI AlwNI ApaI ApaLI
ApoI AspHI AvaI AvaII BanI
BanII BbrPI BbvCI BbvI BcnI
BfaI BlpI BmyI BpmI Bpu1102I
BsaAI BsaI BsaJI BseRI BsgI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsmI BsoBI Bsp1286I BspMI BsrI
BssKI BssSI BstAPI BstF5I BstNI
Cac8I CelII CfoI Cfr13I DdeI
DpnII DraII DraIII DsaI EaeI
EarI EcoNI EcoO109I EcoRII EcoT14I
FauI Fnu4HI FokI HaeII HaeIII
HapII HgaI HhaI HinP1I HinfI
HpaII HphI ItaI Ksp632I MaeI
MaeII MaeIII MboI MboII MnlI
MseI MslI MspA1I MspI MvaI
MwoI NciI NcoI NdeII NlaIII
NlaIV NspI PflMI PleI PmaCI
PmlI PpuMI PstI PvuII RsaI
Sau3AI Sau96I ScrFI SfaNI SfcI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I Van91I
XcmI