##################################
# NO CUT ENZYMES ( 84 enzymes) #
##################################
AatII AccIII AclI AcyI AgeI
AhdI Aor51HI ApaI AscI AspEI
AspI BamHI BbeI BbrPI BcgI
BsaAI BsaBI BsaHI BsaWI BseAI
BsiWI BsmBI Bsp1407I BspDI BspEI
BspMI BsrGI BssHII Bst1107I BstBI
BstEII BstPI BstZ17I ClaI DraIII
Eam1105I Eco47III EcoNI EcoO65I EcoRI
EcoRV EcoT22I FseI HgaI Hin1I
KasI KspI MamI MfeI MluI
MroI MunI NarI NotI NruI
NsiI NspV PaeR7I PflFI PflMI
PinAI PmaCI PmeI PmlI Psp1406I
PvuI SacI SacII SalI SbfI
ScaI SfiI SfoI SfuI SgrAI
SnaBI SphI Sse8387I SspBI StuI
SwaI Tth111I Van91I XhoI
###################################
# ONE CUT ENZYMES ( 33 enzymes) #
###################################
Acc65I 4689 GGTACC G^GTACC
AccI 2468 GTAGAC GT^[AC][GT]AC
AflII 1108 CTTAAG C^TTAAG
Alw44I 1917 GTGCAC G^TGCAC
AlwI 1034 GATCC GGATC(4/5)
ApaLI 1917 GTGCAC G^TGCAC
AseI 1940 ATTAAT AT^TAAT
AsnI 1940 ATTAAT AT^TAAT
Asp718I 4689 GGTACC G^GTACC
AviII 852 TGCGCA TGC^GCA
BciVI 3965 GGATAC GTATCC(6/5)
BfrI 1108 CTTAAG C^TTAAG
BspHI 2353 TCATGA T^CATGA
BstAPI 1379 GCAGATTCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
CpoI 196 CGGACCG CG^G[AT]CCG
FspI 852 TGCGCA TGC^GCA
HaeII 162 GGCGCT [GA]GCGC^[CT]
KpnI 4689 GGTACC GGTAC^C
NaeI 131 GCCGGC GCC^GGC
NgoMIV 131 GCCGGC G^CCGGC
NheI 2422 GCTAGC G^CTAGC
PacI 3059 TTAATTAA TTAAT^TAA
PpuMI 1805 GGGTCCC [GA]G^G[AT]CC[CT]
PshBI 1940 ATTAAT AT^TAAT
RcaI 2353 TCATGA T^CATGA
RsrII 196 CGGACCG CG^G[AT]CCG
SapI 901 GCTCTTC GCTCTTC(1/4)
SexAI 4962 ACCTGGT A^CC[AT]GGT
SpeI 2712 ACTAGT A^CTAGT
TaqI 2632 TCGA T^CGA
TthHB8I 2632 TCGA T^CGA
XbaI 2126 TCTAGA T^CTAGA
XcmI 2369 CCAGATCTTCCTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 24 enzymes) #
###################################
AlwNI 1380 CAGATTCTG CAG[ACGT][ACGT][ACGT]^CTG
3723 CAGCCTCTG
AocI 143 CCTGAGG CC^T[ACGT]AGG
1594 CCTGAGG
BalI 952 TGGCCA TGG^CCA
3792 TGGCCA
BanI 1298 GGTGCC G^G[CT][GA]CC
4689 GGTACC
BclI 4016 TGATCA T^GATCA
4411 TGATCA
BglI 15 GCCGGTGCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
2293 GCCATGGAGGC
BlpI 1734 GCTCAGC GC^T[ACGT]AGC
4349 GCTTAGC
Bpu1102I 1734 GCTCAGC GC^T[ACGT]AGC
4349 GCTTAGC
BsaI 805 GAGACC GGTCTC(1/5)
3154 GAGACC
BsrBI 509 CCGCTC CCGCTC(-3/-3)
1076 GAGCGG
BsrDI 1980 CATTGC GCAATG(2/0)
4012 GCAATG
BstXI 1122 CCACAGGAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1392 CCACTGTTCTGG
Bsu36I 143 CCTGAGG CC^T[ACGT]AGG
1594 CCTGAGG
CelII 1734 GCTCAGC GC^T[ACGT]AGC
4349 GCTTAGC
DrdI 869 GACAAACATGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3502 GACAGCCAGGTC
EarI 902 CTCTTC CTCTTC(1/4)
2266 GAAGAG
Eco81I 143 CCTGAGG CC^T[ACGT]AGG
1594 CCTGAGG
FbaI 4016 TGATCA T^GATCA
4411 TGATCA
Ksp632I 902 CTCTTC CTCTTC(1/4)
2266 GAAGAG
MluNI 952 TGGCCA TGG^CCA
3792 TGGCCA
MscI 952 TGGCCA TGG^CCA
3792 TGGCCA
NdeI 2484 CATATG CA^TATG
2922 CATATG
PshAI 519 GACCGCTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
3156 GACCGCTGTC
SmlI 1108 CTTAAG C^T[CT][GA]AG
2211 CTCAAG
#########################################
# 3 OR MORE CUT ENZYMES ( 117 enzymes) #
#########################################
AccII AciI AcsI AfaI AflIII
AluI ApoI Asp700I AspHI AvaI
AvaII AvrII BanII BbsI BbvCI
BbvI BcnI BfaI BglII BlnI
BmyI BpmI BpuAI BsaJI BseRI
BsgI BsiEI BsiHKAI BsiYI BslI
BsmAI BsmFI BsmI BsoBI Bsp1286I
BspLU11I BsrFI BsrI BssKI BssSI
BstF5I BstNI BstUI BstYI Cac8I
CfoI Cfr10I Cfr13I DdeI DpnII
DraI DraII DsaI EaeI EagI
EclXI Eco52I EcoO109I EcoRII EcoT14I
FauI Fnu4HI FokI HaeIII HapII
HhaI HinP1I HincII HindII HindIII
HinfI HpaI HpaII HphI ItaI
MaeI MaeII MaeIII MboI MboII
MflI MnlI MseI MslI MspA1I
MspI MvaI MvnI MwoI NciI
NcoI NdeII NlaIII NlaIV NspI
PleI PstI PvuII RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI SmaI
SspI StyI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI XhoII XmaCI
XmaI XmnI