##################################
# NO CUT ENZYMES (   68 enzymes) #
##################################

AatII       AccI        AhdI        Aor51HI     AscI        
AseI        AsnI        AspEI       AviII       BamHI       
BbrPI       BcgI        BlpI        Bpu1102I    BsaAI       
BsaBI       BsiEI       BsmBI       BspHI       BssHII      
BssSI       Bst1107I    BstEII      BstPI       BstZ17I     
CelII       CpoI        DrdI        EagI        Eam1105I    
EclXI       Eco47III    Eco52I      EcoO65I     EcoT22I     
FseI        FspI        HindIII     HpaI        KspI        
MamI        MfeI        MluI        MunI        NdeI        
NheI        NotI        NruI        NsiI        PacI        
PaeR7I      PmaCI       PmeI        PmlI        PshBI       
PvuI        RcaI        RsrII       SacII       SalI        
ScaI        SfiI        SgrAI       SnaBI       SpeI        
SspI        SwaI        XhoI        


###################################
# ONE CUT ENZYMES (   44 enzymes) #
###################################

AccII        2420  CGCG                 CG^CG
AccIII       1088  TCCGGA               T^CCGGA
AclI         2344  AACGTT               AA^CGTT
AflII         944  CTTAAG               C^TTAAG
AgeI           25  ACCGGT               A^CCGGT
ApaI         2869  GGGCCC               GGGCC^C
AvrII          70  CCTAGG               C^CTAGG
BbvCI        1871  CCTCAGC              CCTCAGC(-5/-2)
BciVI         492  GTATCC               GTATCC(6/5)
BclI          888  TGATCA               T^GATCA
BfrI          944  CTTAAG               C^TTAAG
BglII        1553  AGATCT               A^GATCT
BlnI           70  CCTAGG               C^CTAGG
BseAI        1088  TCCGGA               T^CCGGA
BsiWI         714  CGTACG               C^GTACG
Bsp1407I      756  TGTACA               T^GTACA
BspDI        1332  ATCGAT               AT^CGAT
BspEI        1088  TCCGGA               T^CCGGA
BspLU11I     3151  ACATGT               A^CATGT
BsrBI         967  CCGCTC               CCGCTC(-3/-3)
BsrGI         756  TGTACA               T^GTACA
BstAPI       2680  GCATCAGATGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI        1286  TTCGAA               TT^CGAA
BstUI        2420  CGCG                 CG^CG
ClaI         1332  ATCGAT               AT^CGAT
DraI         4665  TTTAAA               TTT^AAA
DraIII       4273  CACCTGGTG            CAC[ACGT][ACGT][ACGT]^GTG
FbaI          888  TGATCA               T^GATCA
HincII       3973  GTCAAC               GT[CT]^[GA]AC
HindII       3973  GTCAAC               GT[CT]^[GA]AC
MroI         1088  TCCGGA               T^CCGGA
MvnI         2420  CGCG                 CG^CG
NaeI          827  GCCGGC               GCC^GGC
NcoI         4516  CCATGG               C^CATGG
NgoMIV        827  GCCGGC               G^CCGGC
NspV         1286  TTCGAA               TT^CGAA
PinAI          25  ACCGGT               A^CCGGT
PshAI        1501  GACCCGTGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
Psp1406I     2344  AACGTT               AA^CGTT
SbfI         3864  CCTGCAGG             CCTGCA^GG
SfuI         1286  TTCGAA               TT^CGAA
Sse8387I     3864  CCTGCAGG             CCTGCA^GG
SspBI         756  TGTACA               T^GTACA
XbaI         3911  TCTAGA               T^CTAGA


###################################
# TWO CUT ENZYMES (   23 enzymes) #
###################################

Acc65I        774  GGTACC               G^GTACC
             3334  GGTACC              
AflIII       3151  ACATGT               A^C[GA][CT]GT
             4477  ACACGT              
Alw44I        542  GTGCAC               G^TGCAC
             1376  GTGCAC              
ApaLI         542  GTGCAC               G^TGCAC
             1376  GTGCAC              
Asp700I      1018  GAAGAAGTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
             4489  GAAAGTGTTC          
Asp718I       774  GGTACC               G^GTACC
             3334  GGTACC              
BbeI          830  GGCGCC               GGCGC^C
             1561  GGCGCC              
BsmI         1913  GAATGC               GAATGC(1/-1)
             2978  GCATTC              
BsrDI        2890  CATTGC               GCAATG(2/0)
             1233  GCAATG              
BsrFI          25  ACCGGT               [GA]^CCGG[CT]
              827  GCCGGC              
Cfr10I         25  ACCGGT               [GA]^CCGG[CT]
              827  GCCGGC              
EcoRI        1678  GAATTC               G^AATTC
             2281  GAATTC              
EcoRV         446  GATATC               GAT^ATC
              681  GATATC              
FauI          168  CCCGC                CCCGC(4/6)
              330  CCCGC               
HgaI         1463  GACGC                GACGC(5/10)
             2909  GCGTC               
KasI          830  GGCGCC               G^GCGCC
             1561  GGCGCC              
KpnI          774  GGTACC               GGTAC^C
             3334  GGTACC              
NarI          830  GGCGCC               GG^CGCC
             1561  GGCGCC              
SacI         3402  GAGCTC               GAGCT^C
             3763  GAGCTC              
SfoI          830  GGCGCC               GGC^GCC
             1561  GGCGCC              
SphI          987  GCATGC               GCATG^C
             2373  GCATGC              
StuI         3512  AGGCCT               AGG^CCT
             4538  AGGCCT              
XmnI         1018  GAAGAAGTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
             4489  GAAAGTGTTC          


#########################################
# 3 OR MORE CUT ENZYMES (  123 enzymes) #
#########################################

AciI        AcsI        AcyI        AfaI        AluI        
AlwI        AlwNI       AocI        ApoI        AspHI       
AspI        AvaI        AvaII       BalI        BanI        
BanII       BbsI        BbvI        BcnI        BfaI        
BglI        BmyI        BpmI        BpuAI       BsaHI       
BsaI        BsaJI       BsaWI       BseRI       BsgI        
BsiHKAI     BsiYI       BslI        BsmAI       BsmFI       
BsoBI       Bsp1286I    BspMI       BsrI        BssKI       
BstF5I      BstNI       BstXI       BstYI       Bsu36I      
Cac8I       CfoI        Cfr13I      DdeI        DpnII       
DraII       DsaI        EaeI        EarI        Eco81I      
EcoNI       EcoO109I    EcoRII      EcoT14I     Fnu4HI      
FokI        HaeII       HaeIII      HapII       HhaI        
Hin1I       HinP1I      HinfI       HpaII       HphI        
ItaI        Ksp632I     MaeI        MaeII       MaeIII      
MboI        MboII       MflI        MluNI       MnlI        
MscI        MseI        MslI        MspA1I      MspI        
MvaI        MwoI        NciI        NdeII       NlaIII      
NlaIV       NspI        PflFI       PflMI       PleI        
PpuMI       PstI        PvuII       RsaI        SapI        
Sau3AI      Sau96I      ScrFI       SexAI       SfaNI       
SfcI        SmaI        SmlI        StyI        TaqI        
TfiI        Tru9I       TseI        Tsp45I      Tsp509I     
TspRI       Tth111I     TthHB8I     Van91I      XcmI        
XhoII       XmaCI       XmaI