##################################
# NO CUT ENZYMES ( 68 enzymes) #
##################################
AatII AccI AhdI Aor51HI AscI
AseI AsnI AspEI AviII BamHI
BbrPI BcgI BlpI Bpu1102I BsaAI
BsaBI BsiEI BsmBI BspHI BssHII
BssSI Bst1107I BstEII BstPI BstZ17I
CelII CpoI DrdI EagI Eam1105I
EclXI Eco47III Eco52I EcoO65I EcoT22I
FseI FspI HindIII HpaI KspI
MamI MfeI MluI MunI NdeI
NheI NotI NruI NsiI PacI
PaeR7I PmaCI PmeI PmlI PshBI
PvuI RcaI RsrII SacII SalI
ScaI SfiI SgrAI SnaBI SpeI
SspI SwaI XhoI
###################################
# ONE CUT ENZYMES ( 44 enzymes) #
###################################
AccII 2420 CGCG CG^CG
AccIII 1088 TCCGGA T^CCGGA
AclI 2344 AACGTT AA^CGTT
AflII 944 CTTAAG C^TTAAG
AgeI 25 ACCGGT A^CCGGT
ApaI 2869 GGGCCC GGGCC^C
AvrII 70 CCTAGG C^CTAGG
BbvCI 1871 CCTCAGC CCTCAGC(-5/-2)
BciVI 492 GTATCC GTATCC(6/5)
BclI 888 TGATCA T^GATCA
BfrI 944 CTTAAG C^TTAAG
BglII 1553 AGATCT A^GATCT
BlnI 70 CCTAGG C^CTAGG
BseAI 1088 TCCGGA T^CCGGA
BsiWI 714 CGTACG C^GTACG
Bsp1407I 756 TGTACA T^GTACA
BspDI 1332 ATCGAT AT^CGAT
BspEI 1088 TCCGGA T^CCGGA
BspLU11I 3151 ACATGT A^CATGT
BsrBI 967 CCGCTC CCGCTC(-3/-3)
BsrGI 756 TGTACA T^GTACA
BstAPI 2680 GCATCAGATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI 1286 TTCGAA TT^CGAA
BstUI 2420 CGCG CG^CG
ClaI 1332 ATCGAT AT^CGAT
DraI 4665 TTTAAA TTT^AAA
DraIII 4273 CACCTGGTG CAC[ACGT][ACGT][ACGT]^GTG
FbaI 888 TGATCA T^GATCA
HincII 3973 GTCAAC GT[CT]^[GA]AC
HindII 3973 GTCAAC GT[CT]^[GA]AC
MroI 1088 TCCGGA T^CCGGA
MvnI 2420 CGCG CG^CG
NaeI 827 GCCGGC GCC^GGC
NcoI 4516 CCATGG C^CATGG
NgoMIV 827 GCCGGC G^CCGGC
NspV 1286 TTCGAA TT^CGAA
PinAI 25 ACCGGT A^CCGGT
PshAI 1501 GACCCGTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
Psp1406I 2344 AACGTT AA^CGTT
SbfI 3864 CCTGCAGG CCTGCA^GG
SfuI 1286 TTCGAA TT^CGAA
Sse8387I 3864 CCTGCAGG CCTGCA^GG
SspBI 756 TGTACA T^GTACA
XbaI 3911 TCTAGA T^CTAGA
###################################
# TWO CUT ENZYMES ( 23 enzymes) #
###################################
Acc65I 774 GGTACC G^GTACC
3334 GGTACC
AflIII 3151 ACATGT A^C[GA][CT]GT
4477 ACACGT
Alw44I 542 GTGCAC G^TGCAC
1376 GTGCAC
ApaLI 542 GTGCAC G^TGCAC
1376 GTGCAC
Asp700I 1018 GAAGAAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
4489 GAAAGTGTTC
Asp718I 774 GGTACC G^GTACC
3334 GGTACC
BbeI 830 GGCGCC GGCGC^C
1561 GGCGCC
BsmI 1913 GAATGC GAATGC(1/-1)
2978 GCATTC
BsrDI 2890 CATTGC GCAATG(2/0)
1233 GCAATG
BsrFI 25 ACCGGT [GA]^CCGG[CT]
827 GCCGGC
Cfr10I 25 ACCGGT [GA]^CCGG[CT]
827 GCCGGC
EcoRI 1678 GAATTC G^AATTC
2281 GAATTC
EcoRV 446 GATATC GAT^ATC
681 GATATC
FauI 168 CCCGC CCCGC(4/6)
330 CCCGC
HgaI 1463 GACGC GACGC(5/10)
2909 GCGTC
KasI 830 GGCGCC G^GCGCC
1561 GGCGCC
KpnI 774 GGTACC GGTAC^C
3334 GGTACC
NarI 830 GGCGCC GG^CGCC
1561 GGCGCC
SacI 3402 GAGCTC GAGCT^C
3763 GAGCTC
SfoI 830 GGCGCC GGC^GCC
1561 GGCGCC
SphI 987 GCATGC GCATG^C
2373 GCATGC
StuI 3512 AGGCCT AGG^CCT
4538 AGGCCT
XmnI 1018 GAAGAAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
4489 GAAAGTGTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 123 enzymes) #
#########################################
AciI AcsI AcyI AfaI AluI
AlwI AlwNI AocI ApoI AspHI
AspI AvaI AvaII BalI BanI
BanII BbsI BbvI BcnI BfaI
BglI BmyI BpmI BpuAI BsaHI
BsaI BsaJI BsaWI BseRI BsgI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsoBI Bsp1286I BspMI BsrI BssKI
BstF5I BstNI BstXI BstYI Bsu36I
Cac8I CfoI Cfr13I DdeI DpnII
DraII DsaI EaeI EarI Eco81I
EcoNI EcoO109I EcoRII EcoT14I Fnu4HI
FokI HaeII HaeIII HapII HhaI
Hin1I HinP1I HinfI HpaII HphI
ItaI Ksp632I MaeI MaeII MaeIII
MboI MboII MflI MluNI MnlI
MscI MseI MslI MspA1I MspI
MvaI MwoI NciI NdeII NlaIII
NlaIV NspI PflFI PflMI PleI
PpuMI PstI PvuII RsaI SapI
Sau3AI Sau96I ScrFI SexAI SfaNI
SfcI SmaI SmlI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI Tth111I TthHB8I Van91I XcmI
XhoII XmaCI XmaI