##################################
# NO CUT ENZYMES (   66 enzymes) #
##################################

AatII       AclI        AflII       AgeI        AhdI        
Alw44I      ApaLI       AscI        AspEI       AviII       
BbrPI       BclI        BfrI        BsaAI       BsiWI       
Bsp1407I    BspDI       BsrGI       BssHII      Bst1107I    
BstAPI      BstBI       BstZ17I     ClaI        CpoI        
DraI        DrdI        Eam1105I    EcoRI       EcoT22I     
FbaI        FseI        FspI        HincII      HindII      
HindIII     HpaI        KspI        MfeI        MluI        
MunI        NheI        NotI        NruI        NsiI        
NspV        PacI        PinAI       PmaCI       PmeI        
PmlI        Psp1406I    PvuI        RsrII       SacII       
SalI        SbfI        SexAI       SfuI        SnaBI       
SpeI        Sse8387I    SspBI       SspI        SwaI        
XbaI        


###################################
# ONE CUT ENZYMES (   52 enzymes) #
###################################

Acc65I       1298  GGTACC               G^GTACC
AccI         2928  GTAGAC               GT^[AC][GT]AC
AccIII         88  TCCGGA               T^CCGGA
AcsI          288  AAATTT               [GA]^AATT[CT]
AcyI         3432  GGCGCC               G[GA]^CG[CT]C
ApoI          288  AAATTT               [GA]^AATT[CT]
AseI         3750  ATTAAT               AT^TAAT
AsnI         3750  ATTAAT               AT^TAAT
Asp700I      1311  GAAGACCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I      1298  GGTACC               G^GTACC
AspI         3081  GACAAAGTC            GAC[ACGT]^[ACGT][ACGT]GTC
AvrII        4408  CCTAGG               C^CTAGG
BalI          263  TGGCCA               TGG^CCA
BbeI         3432  GGCGCC               GGCGC^C
BcgI          436  GCAAAGCCATCG         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BglII        2269  AGATCT               A^GATCT
BlnI         4408  CCTAGG               C^CTAGG
BlpI          699  GCTGAGC              GC^T[ACGT]AGC
Bpu1102I      699  GCTGAGC              GC^T[ACGT]AGC
BsaHI        3432  GGCGCC               G[GA]^CG[CT]C
BsaI         2186  GGTCTC               GGTCTC(1/5)
BseAI          88  TCCGGA               T^CCGGA
BspEI          88  TCCGGA               T^CCGGA
BspHI        4768  TCATGA               T^CATGA
BspLU11I      316  ACATGT               A^CATGT
BssSI        2922  CACGAG               CACGAG(-5/-1)
BstEII       2663  GGTGACC              G^GT[ACGT]ACC
BstPI        2663  GGTGACC              G^GT[ACGT]ACC
CelII         699  GCTGAGC              GC^T[ACGT]AGC
EagI         1425  CGGCCG               C^GGCCG
EclXI        1425  CGGCCG               C^GGCCG
Eco52I       1425  CGGCCG               C^GGCCG
EcoO65I      2663  GGTGACC              G^GT[ACGT]ACC
EcoRV        4322  GATATC               GAT^ATC
HgaI          412  GCGTC                GACGC(5/10)
Hin1I        3432  GGCGCC               G[GA]^CG[CT]C
KasI         3432  GGCGCC               G^GCGCC
KpnI         1298  GGTACC               GGTAC^C
MluNI         263  TGGCCA               TGG^CCA
MroI           88  TCCGGA               T^CCGGA
MscI          263  TGGCCA               TGG^CCA
NarI         3432  GGCGCC               GG^CGCC
NdeI         5016  CATATG               CA^TATG
PflFI        3081  GACAAAGTC            GAC[ACGT]^[ACGT][ACGT]GTC
PshAI        2871  GACCCCAGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI        3750  ATTAAT               AT^TAAT
RcaI         4768  TCATGA               T^CATGA
SapI          512  GCTCTTC              GCTCTTC(1/4)
SfoI         3432  GGCGCC               GGC^GCC
SgrAI        1200  CACCGGCG             C[GA]^CCGG[CT]G
Tth111I      3081  GACAAAGTC            GAC[ACGT]^[ACGT][ACGT]GTC
XmnI         1311  GAAGACCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   19 enzymes) #
###################################

AccII         199  CGCG                 CG^CG
             2115  CGCG                
AflIII        316  ACATGT               A^C[GA][CT]GT
              688  ACACGT              
Aor51HI       457  AGCGCT               AGC^GCT
              553  AGCGCT              
BbsI         1311  GAAGAC               GAAGAC(2/6)
             4800  GAAGAC              
BciVI        3643  GGATAC               GTATCC(6/5)
              613  GTATCC              
BpuAI        1311  GAAGAC               GAAGAC(2/6)
             4800  GAAGAC              
BsaBI        1395  GATGATTATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
             3290  GATTGAAATC          
BsiEI        1425  CGGCCG               CG[GA][CT]^CG
             3313  CGACCG              
BsmBI        1174  CGTCTC               CGTCTC(1/5)
             4847  GAGACG              
BstUI         199  CGCG                 CG^CG
             2115  CGCG                
BstXI        1118  CCAGCCCAATGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             4836  CCACCCACCTGG        
Eco47III      457  AGCGCT               AGC^GCT
              553  AGCGCT              
MamI         1395  GATGATTATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
             3290  GATTGAAATC          
MvnI          199  CGCG                 CG^CG
             2115  CGCG                
NaeI         1150  GCCGGC               GCC^GGC
             1448  GCCGGC              
NgoMIV       1150  GCCGGC               G^CCGGC
             1448  GCCGGC              
ScaI         1363  AGTACT               AGT^ACT
             1405  AGTACT              
SfiI          982  GGCCTGAGAGGCC        GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
             2855  GGCCAGCCCGGCC       
XcmI          370  CCAACCACAACATGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
             4737  CCAGACGAGGACTGG     


#########################################
# 3 OR MORE CUT ENZYMES (  121 enzymes) #
#########################################

AciI        AfaI        AluI        AlwI        AlwNI       
AocI        ApaI        AspHI       AvaI        AvaII       
BamHI       BanI        BanII       BbvCI       BbvI        
BcnI        BfaI        BglI        BmyI        BpmI        
BsaJI       BsaWI       BseRI       BsgI        BsiHKAI     
BsiYI       BslI        BsmAI       BsmFI       BsmI        
BsoBI       Bsp1286I    BspMI       BsrBI       BsrDI       
BsrFI       BsrI        BssKI       BstF5I      BstNI       
BstYI       Bsu36I      Cac8I       CfoI        Cfr10I      
Cfr13I      DdeI        DpnII       DraII       DraIII      
DsaI        EaeI        EarI        Eco81I      EcoNI       
EcoO109I    EcoRII      EcoT14I     FauI        Fnu4HI      
FokI        HaeII       HaeIII      HapII       HhaI        
HinP1I      HinfI       HpaII       HphI        ItaI        
Ksp632I     MaeI        MaeII       MaeIII      MboI        
MboII       MflI        MnlI        MseI        MslI        
MspA1I      MspI        MvaI        MwoI        NciI        
NcoI        NdeII       NlaIII      NlaIV       NspI        
PaeR7I      PflMI       PleI        PpuMI       PstI        
PvuII       RsaI        SacI        Sau3AI      Sau96I      
ScrFI       SfaNI       SfcI        SmaI        SmlI        
SphI        StuI        StyI        TaqI        TfiI        
Tru9I       TseI        Tsp45I      Tsp509I     TspRI       
TthHB8I     Van91I      XhoI        XhoII       XmaCI       
XmaI