##################################
# NO CUT ENZYMES ( 81 enzymes) #
##################################
AatII Acc65I AclI AgeI AhdI
AocI Aor51HI ApaI AscI Asp718I
AspEI AspI AviII AvrII BalI
BamHI BbrPI BbvCI BglI BlnI
BsaAI BsiWI BsmBI BspDI BsrBI
BsrFI BssHII Bst1107I BstAPI BstBI
BstZ17I Bsu36I Cfr10I ClaI CpoI
DrdI EagI Eam1105I EclXI Eco47III
Eco52I Eco81I EcoT22I FseI FspI
HpaI KpnI MluI MluNI MscI
NaeI NgoMIV NotI NruI NsiI
NspV PacI PflFI PinAI PmaCI
PmeI PmlI PshAI Psp1406I PvuI
RsrII SacI SalI SbfI ScaI
SexAI SfiI SfuI SgrAI SmaI
SnaBI Sse8387I SwaI Tth111I XmaCI
XmaI
###################################
# ONE CUT ENZYMES ( 54 enzymes) #
###################################
AccIII 89 TCCGGA T^CCGGA
AcyI 247 GGCGCC G[GA]^CG[CT]C
AflII 882 CTTAAG C^TTAAG
AflIII 5346 ACATGT A^C[GA][CT]GT
Alw44I 1336 GTGCAC G^TGCAC
ApaLI 1336 GTGCAC G^TGCAC
AvaI 2744 CTCGAG C^[CT]CG[GA]G
BanI 247 GGCGCC G^G[CT][GA]CC
BbeI 247 GGCGCC GGCGC^C
BfrI 882 CTTAAG C^TTAAG
BlpI 980 GCTTAGC GC^T[ACGT]AGC
Bpu1102I 980 GCTTAGC GC^T[ACGT]AGC
BsaHI 247 GGCGCC G[GA]^CG[CT]C
BsaI 278 GAGACC GGTCTC(1/5)
BsaWI 89 TCCGGA [AT]^CCGG[AT]
BseAI 89 TCCGGA T^CCGGA
BsgI 334 GTGCAG GTGCAG(16/14)
BsiEI 137 CGGTCG CG[GA][CT]^CG
BsmFI 967 GTCCC GGGAC(10/14)
BsoBI 2744 CTCGAG C^[CT]CG[GA]G
BspEI 89 TCCGGA T^CCGGA
BspHI 2856 TCATGA T^CATGA
BspLU11I 5346 ACATGT A^CATGT
BspMI 4393 GCAGGT ACCTGC(4/8)
BssSI 3393 CACGAG CACGAG(-5/-1)
BstEII 6 GGTAACC G^GT[ACGT]ACC
BstPI 6 GGTAACC G^GT[ACGT]ACC
BstXI 3063 CCAGTATAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CelII 980 GCTTAGC GC^T[ACGT]AGC
DraIII 4973 CACCTTGTG CAC[ACGT][ACGT][ACGT]^GTG
EaeI 57 CGGCCA [CT]^GGCC[GA]
EcoO65I 6 GGTAACC G^GT[ACGT]ACC
EcoRV 1625 GATATC GAT^ATC
HaeII 247 GGCGCC [GA]GCGC^[CT]
HgaI 2725 GACGC GACGC(5/10)
Hin1I 247 GGCGCC G[GA]^CG[CT]C
HincII 4894 GTCAAC GT[CT]^[GA]AC
HindII 4894 GTCAAC GT[CT]^[GA]AC
KasI 247 GGCGCC G^GCGCC
KspI 32 CCGCGG CCGC^GG
MfeI 3952 CAATTG C^AATTG
MroI 89 TCCGGA T^CCGGA
MunI 3952 CAATTG C^AATTG
NarI 247 GGCGCC GG^CGCC
NdeI 1034 CATATG CA^TATG
NheI 4884 GCTAGC G^CTAGC
PaeR7I 2744 CTCGAG C^TCGAG
RcaI 2856 TCATGA T^CATGA
SacII 32 CCGCGG CCGC^GG
SfoI 247 GGCGCC GGC^GCC
SpeI 4315 ACTAGT A^CTAGT
SphI 1592 GCATGC GCATG^C
StuI 2131 AGGCCT AGG^CCT
XhoI 2744 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 20 enzymes) #
###################################
AccI 4406 GTCTAC GT^[AC][GT]AC
5190 GTCTAC
AlwNI 3980 CAGAAGCTG CAG[ACGT][ACGT][ACGT]^CTG
4165 CAGTCACTG
Asp700I 1111 GAAAGCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
3007 GAAGACCTTC
BanII 85 GGGCTC G[GA]GC[CT]^C
189 GGGCTC
BcgI 2503 CGAGAACTGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
2736 GCAACTTTCTCG
BsmI 2754 GAATGC GAATGC(1/-1)
3099 GAATGC
BsrDI 1248 CATTGC GCAATG(2/0)
3103 GCAATG
DraII 2978 AGGACCT [GA]G^G[ACGT]CC[CT]
3443 AGGACCT
EcoNI 43 CCTTTGGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
881 CCTTAAGCAGG
EcoO109I 2978 AGGACCT [GA]G^G[ACGT]CC[CT]
3443 AGGACCT
HindIII 978 AAGCTT A^AGCTT
4024 AAGCTT
NcoI 582 CCATGG C^CATGG
4299 CCATGG
PflMI 2041 CCAGTGTTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2658 CCAAACACTGG
PpuMI 2978 AGGACCT [GA]G^G[AT]CC[CT]
3443 AGGACCT
PstI 1469 CTGCAG CTGCA^G
4391 CTGCAG
PvuII 1712 CAGCTG CAG^CTG
3092 CAGCTG
Van91I 2041 CCAGTGTTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2658 CCAAACACTGG
XbaI 3178 TCTAGA T^CTAGA
4824 TCTAGA
XcmI 4901 CCAAACTTCTTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
4955 CCAGGCCAGTCATGG
XmnI 1111 GAAAGCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
3007 GAAGACCTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 103 enzymes) #
#########################################
AccII AciI AcsI AfaI AluI
AlwI ApoI AseI AsnI AspHI
AvaII BbsI BbvI BciVI BclI
BcnI BfaI BglII BmyI BpmI
BpuAI BsaBI BsaJI BseRI BsiHKAI
BsiYI BslI BsmAI Bsp1286I Bsp1407I
BsrGI BsrI BssKI BstF5I BstNI
BstUI BstYI Cac8I CfoI Cfr13I
DdeI DpnII DraI DsaI EarI
EcoRI EcoRII EcoT14I FauI FbaI
Fnu4HI FokI HaeIII HapII HhaI
HinP1I HinfI HpaII HphI ItaI
Ksp632I MaeI MaeII MaeIII MamI
MboI MboII MflI MnlI MseI
MslI MspA1I MspI MvaI MvnI
MwoI NciI NdeII NlaIII NlaIV
NspI PleI PshBI RsaI SapI
Sau3AI Sau96I ScrFI SfaNI SfcI
SmlI SspBI SspI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I XhoII