##################################
# NO CUT ENZYMES (   85 enzymes) #
##################################

AatII       Acc65I      AccII       AgeI        AhdI        
AocI        Aor51HI     AscI        Asp718I     AspEI       
AspI        AvrII       BbeI        BbrPI       BbvCI       
BcgI        BciVI       BcnI        BglI        BlnI        
BsiEI       BsiWI       BspDI       BsrFI       BssHII      
BstBI       BstEII      BstPI       BstUI       Bsu36I      
Cfr10I      ClaI        CpoI        EagI        Eam1105I    
EclXI       Eco47III    Eco52I      Eco81I      EcoNI       
EcoO65I     EcoRV       FseI        HaeII       KasI        
KpnI        KspI        MluI        MvnI        NaeI        
NarI        NciI        NgoMIV      NheI        NotI        
NruI        NspV        PacI        PaeR7I      PflFI       
PinAI       PmaCI       PmeI        PmlI        PpuMI       
PvuI        RsrII       SacI        SacII       SalI        
SapI        SbfI        ScaI        SfiI        SfoI        
SfuI        SgrAI       SmaI        SnaBI       Sse8387I    
Tth111I     XcmI        XhoI        XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   54 enzymes) #
###################################

AccIII       1621  TCCGGA               T^CCGGA
AclI          331  AACGTT               AA^CGTT
AcyI         3044  GGCGTC               G[GA]^CG[CT]C
AflII        2028  CTTAAG               C^TTAAG
Alw44I       3520  GTGCAC               G^TGCAC
ApaLI        3520  GTGCAC               G^TGCAC
AvaI          611  CTCGGG               C^[CT]CG[GA]G
AvaII        2815  GGACC                G^G[AT]CC
AviII        4450  TGCGCA               TGC^GCA
BalI         1549  TGGCCA               TGG^CCA
BclI         2581  TGATCA               T^GATCA
BfrI         2028  CTTAAG               C^TTAAG
BglII         104  AGATCT               A^GATCT
BsaAI        4332  TACGTG               [CT]AC^GT[GA]
BsaHI        3044  GGCGTC               G[GA]^CG[CT]C
BsaI         3175  GAGACC               GGTCTC(1/5)
BseAI        1621  TCCGGA               T^CCGGA
BsmBI         855  GAGACG               CGTCTC(1/5)
BsoBI         611  CTCGGG               C^[CT]CG[GA]G
BspEI        1621  TCCGGA               T^CCGGA
BsrBI        3456  GAGCGG               CCGCTC(-3/-3)
Bst1107I     4306  GTATAC               GTA^TAC
BstZ17I      4306  GTATAC               GTA^TAC
CfoI         4451  GCGC                 GCG^C
DraIII       2551  CACGCAGTG            CAC[ACGT][ACGT][ACGT]^GTG
DrdI         1063  GACAAAGGAGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI          463  CCATGG               C^C[GA][CT]GG
EaeI         1549  TGGCCA               [CT]^GGCC[GA]
EcoRI        3013  GAATTC               G^AATTC
FauI         3458  GCGGG                CCCGC(4/6)
FbaI         2581  TGATCA               T^GATCA
FspI         4450  TGCGCA               TGC^GCA
HgaI         3045  GCGTC                GACGC(5/10)
HhaI         4451  GCGC                 GCG^C
Hin1I        3044  GGCGTC               G[GA]^CG[CT]C
HinP1I       4451  GCGC                 G^CGC
HincII         32  GTTAAC               GT[CT]^[GA]AC
HindII         32  GTTAAC               GT[CT]^[GA]AC
HindIII      3610  AAGCTT               A^AGCTT
HpaI           32  GTTAAC               GTT^AAC
MluNI        1549  TGGCCA               TGG^CCA
MroI         1621  TCCGGA               T^CCGGA
MscI         1549  TGGCCA               TGG^CCA
NcoI          463  CCATGG               C^CATGG
NdeI         2577  CATATG               CA^TATG
PflMI        2510  CCAACTTTTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PshAI        2816  GACCAAGGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
Psp1406I      331  AACGTT               AA^CGTT
PvuII        3555  CAGCTG               CAG^CTG
SexAI        1933  ACCTGGT              A^CC[AT]GGT
SpeI         1497  ACTAGT               A^CTAGT
StuI         2400  AGGCCT               AGG^CCT
SwaI         4802  ATTTAAAT             ATTT^AAAT
Van91I       2510  CCAACTTTTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG


###################################
# TWO CUT ENZYMES (   24 enzymes) #
###################################

AccI         2526  GTAGAC               GT^[AC][GT]AC
             4306  GTATAC              
ApaI         2726  GGGCCC               GGGCC^C
             4086  GGGCCC              
AseI         1974  ATTAAT               AT^TAAT
             2676  ATTAAT              
AsnI         1974  ATTAAT               AT^TAAT
             2676  ATTAAT              
BamHI        2489  GGATCC               G^GATCC
             3629  GGATCC              
BanI         3917  GGTGCC               G^G[CT][GA]CC
             4882  GGTGCC              
BanII        2726  GGGCCC               G[GA]GC[CT]^C
             4086  GGGCCC              
BsaBI         564  GATTTTGATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
              837  GATATAGATC          
BsaWI        1558  ACCGGA               [AT]^CCGG[AT]
             1621  TCCGGA              
BsmI          674  GAATGC               GAATGC(1/-1)
              896  GAATGC              
BspHI        1025  TCATGA               T^CATGA
             2884  TCATGA              
BssSI         253  CACGAG               CACGAG(-5/-1)
             3733  CTCGTG              
BstXI        3025  CCAATTTAGTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             3286  CCAAAATAATGG        
HapII        1559  CCGG                 C^CGG
             1622  CCGG                
HpaII        1559  CCGG                 C^CGG
             1622  CCGG                
MamI          564  GATTTTGATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
              837  GATATAGATC          
MfeI         3211  CAATTG               C^AATTG
             3220  CAATTG              
MspA1I       3555  CAGCTG               C[AC]G^C[GT]G
             4155  CAGCGG              
MspI         1559  CCGG                 C^CGG
             1622  CCGG                
MunI         3211  CAATTG               C^AATTG
             3220  CAATTG              
PshBI        1974  ATTAAT               AT^TAAT
             2676  ATTAAT              
RcaI         1025  TCATGA               T^CATGA
             2884  TCATGA              
SphI         2168  GCATGC               GCATG^C
             2980  GCATGC              
XbaI         1059  TCTAGA               T^CTAGA
             2054  TCTAGA              


#########################################
# 3 OR MORE CUT ENZYMES (   95 enzymes) #
#########################################

AciI        AcsI        AfaI        AflIII      AluI        
AlwI        AlwNI       ApoI        Asp700I     AspHI       
BbsI        BbvI        BfaI        BlpI        BmyI        
BpmI        Bpu1102I    BpuAI       BsaJI       BseRI       
BsgI        BsiHKAI     BsiYI       BslI        BsmAI       
BsmFI       Bsp1286I    Bsp1407I    BspLU11I    BspMI       
BsrDI       BsrGI       BsrI        BssKI       BstAPI      
BstF5I      BstNI       BstYI       Cac8I       CelII       
Cfr13I      DdeI        DpnII       DraI        DraII       
EarI        EcoO109I    EcoRII      EcoT14I     EcoT22I     
Fnu4HI      FokI        HaeIII      HinfI       HphI        
ItaI        Ksp632I     MaeI        MaeII       MaeIII      
MboI        MboII       MflI        MnlI        MseI        
MslI        MvaI        MwoI        NdeII       NlaIII      
NlaIV       NsiI        NspI        PleI        PstI        
RsaI        Sau3AI      Sau96I      ScrFI       SfaNI       
SfcI        SmlI        SspBI       SspI        StyI        
TaqI        TfiI        Tru9I       TseI        Tsp45I      
Tsp509I     TspRI       TthHB8I     XhoII       XmnI