##################################
# NO CUT ENZYMES ( 79 enzymes) #
##################################
AatII AccII AhdI Aor51HI ApaI
AscI AseI AsnI AspEI AviII
AvrII BamHI BbeI BbrPI BclI
BglI BlnI BsaBI BsmBI Bsp1407I
BspDI BsrBI BsrGI BssHII BssSI
Bst1107I BstBI BstEII BstPI BstUI
BstZ17I ClaI CpoI DrdI EagI
Eam1105I EclXI Eco47III Eco52I EcoO65I
EcoRV EcoT22I FbaI FseI FspI
KasI KspI MamI MfeI MluI
MunI MvnI NaeI NarI NgoMIV
NheI NotI NruI NsiI NspV
PacI PmaCI PmeI PmlI PshBI
PvuI RsrII SacII SbfI ScaI
SfiI SfoI SfuI SgrAI SnaBI
Sse8387I SspBI SspI SwaI
###################################
# ONE CUT ENZYMES ( 53 enzymes) #
###################################
AccI 2664 GTCGAC GT^[AC][GT]AC
AccIII 3613 TCCGGA T^CCGGA
AclI 4153 AACGTT AA^CGTT
AcyI 55 GACGCC G[GA]^CG[CT]C
AflII 1426 CTTAAG C^TTAAG
AgeI 2596 ACCGGT A^CCGGT
BalI 921 TGGCCA TGG^CCA
BbvCI 2602 CCTCAGC CCTCAGC(-5/-2)
BcgI 131 GCAGCCGCTTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfrI 1426 CTTAAG C^TTAAG
BglII 1433 AGATCT A^GATCT
BlpI 1450 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 1450 GCTGAGC GC^T[ACGT]AGC
BsaAI 39 CACGTA [CT]AC^GT[GA]
BsaHI 55 GACGCC G[GA]^CG[CT]C
BsaI 787 GGTCTC GGTCTC(1/5)
BseAI 3613 TCCGGA T^CCGGA
BsgI 36 CTGCAC GTGCAG(16/14)
BsiEI 2666 CGACCG CG[GA][CT]^CG
BsiWI 41 CGTACG C^GTACG
BspEI 3613 TCCGGA T^CCGGA
BspLU11I 3421 ACATGT A^CATGT
BspMI 116 ACCTGC ACCTGC(4/8)
BstAPI 257 GCAGGCACTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstXI 2147 CCAGCGGAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CelII 1450 GCTGAGC GC^T[ACGT]AGC
CfoI 2624 GCGC GCG^C
EaeI 921 TGGCCA [CT]^GGCC[GA]
EcoNI 3362 CCTAAAGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
HaeII 2623 AGCGCC [GA]GCGC^[CT]
HgaI 55 GACGC GACGC(5/10)
HhaI 2624 GCGC GCG^C
Hin1I 55 GACGCC G[GA]^CG[CT]C
HinP1I 2624 GCGC G^CGC
HindIII 1714 AAGCTT A^AGCTT
MluNI 921 TGGCCA TGG^CCA
MroI 3613 TCCGGA T^CCGGA
MscI 921 TGGCCA TGG^CCA
NcoI 3544 CCATGG C^CATGG
NdeI 2686 CATATG CA^TATG
PaeR7I 794 CTCGAG C^TCGAG
PinAI 2596 ACCGGT A^CCGGT
PshAI 403 GACACAGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
Psp1406I 4153 AACGTT AA^CGTT
SalI 2664 GTCGAC G^TCGAC
SapI 1342 GAAGAGC GCTCTTC(1/4)
SexAI 203 ACCAGGT A^CC[AT]GGT
SmaI 59 CCCGGG CCC^GGG
SpeI 3225 ACTAGT A^CTAGT
XbaI 4678 TCTAGA T^CTAGA
XhoI 794 CTCGAG C^TCGAG
XmaCI 59 CCCGGG C^CCGGG
XmaI 59 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 29 enzymes) #
###################################
Acc65I 2672 GGTACC G^GTACC
2785 GGTACC
Alw44I 271 GTGCAC G^TGCAC
577 GTGCAC
AocI 1313 CCTCAGG CC^T[ACGT]AGG
1507 CCTCAGG
ApaLI 271 GTGCAC G^TGCAC
577 GTGCAC
Asp718I 2672 GGTACC G^GTACC
2785 GGTACC
AspI 153 GACCGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
3895 GACAAAGTC
AvaI 59 CCCGGG C^[CT]CG[GA]G
794 CTCGAG
BanI 2672 GGTACC G^G[CT][GA]CC
2785 GGTACC
BsmI 561 GAATGC GAATGC(1/-1)
3530 GCATTC
BsoBI 59 CCCGGG C^[CT]CG[GA]G
794 CTCGAG
BspHI 1023 TCATGA T^CATGA
3731 TCATGA
BsrFI 2319 ACCGGC [GA]^CCGG[CT]
2596 ACCGGT
Bsu36I 1313 CCTCAGG CC^T[ACGT]AGG
1507 CCTCAGG
Cfr10I 2319 ACCGGC [GA]^CCGG[CT]
2596 ACCGGT
DraIII 202 CACCAGGTG CAC[ACGT][ACGT][ACGT]^GTG
4200 CACCAAGTG
DsaI 542 CCGTGG C^C[GA][CT]GG
3544 CCATGG
Eco81I 1313 CCTCAGG CC^T[ACGT]AGG
1507 CCTCAGG
EcoRI 1909 GAATTC G^AATTC
3641 GAATTC
HpaI 2969 GTTAAC GTT^AAC
4220 GTTAAC
KpnI 2672 GGTACC GGTAC^C
2785 GGTACC
PflFI 153 GACCGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
3895 GACAAAGTC
PflMI 2339 CCAGACCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3798 CCATAAAATGG
PpuMI 113 AGGACCT [GA]G^G[AT]CC[CT]
2961 AGGACCC
RcaI 1023 TCATGA T^CATGA
3731 TCATGA
SacI 1453 GAGCTC GAGCT^C
3937 GAGCTC
SphI 2065 GCATGC GCATG^C
2413 GCATGC
StuI 569 AGGCCT AGG^CCT
2755 AGGCCT
Tth111I 153 GACCGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
3895 GACAAAGTC
Van91I 2339 CCAGACCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3798 CCATAAAATGG
#########################################
# 3 OR MORE CUT ENZYMES ( 97 enzymes) #
#########################################
AciI AcsI AfaI AflIII AluI
AlwI AlwNI ApoI Asp700I AspHI
AvaII BanII BbsI BbvI BciVI
BcnI BfaI BmyI BpmI BpuAI
BsaJI BsaWI BseRI BsiHKAI BsiYI
BslI BsmAI BsmFI Bsp1286I BsrDI
BsrI BssKI BstF5I BstNI BstYI
Cac8I Cfr13I DdeI DpnII DraI
DraII EarI EcoO109I EcoRII EcoT14I
FauI Fnu4HI FokI HaeIII HapII
HincII HindII HinfI HpaII HphI
ItaI Ksp632I MaeI MaeII MaeIII
MboI MboII MflI MnlI MseI
MslI MspA1I MspI MvaI MwoI
NciI NdeII NlaIII NlaIV NspI
PleI PstI PvuII RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI SmlI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I XcmI
XhoII XmnI