##################################
# NO CUT ENZYMES ( 94 enzymes) #
##################################
AatII Acc65I AccIII AclI AcyI
AgeI AhdI AocI ApaI AscI
Asp718I AspEI AspI AvrII BanI
BbeI BcgI BcnI BglI BlnI
BsaBI BsaHI BsaI BsaWI BseAI
BseRI BsiEI BsiWI BspDI BspEI
BspMI BsrBI BsrFI BssHII BssSI
Bst1107I BstBI BstZ17I Bsu36I Cfr10I
ClaI CpoI DraIII DrdI EagI
Eam1105I EclXI Eco52I Eco81I EcoNI
EcoRI FseI HapII HgaI Hin1I
HpaII KasI KpnI MamI MluI
MroI MspI NaeI NarI NciI
NgoMIV NheI NotI NruI NspV
PflFI PinAI PmeI PshAI Psp1406I
PvuI RsrII SalI SbfI SexAI
SfiI SfoI SfuI SgrAI SmaI
SnaBI SphI Sse8387I StuI SwaI
Tth111I XbaI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 51 enzymes) #
###################################
AccII 1755 CGCG CG^CG
AflII 1031 CTTAAG C^TTAAG
Alw44I 1844 GTGCAC G^TGCAC
AlwNI 1610 CAGCTTCTG CAG[ACGT][ACGT][ACGT]^CTG
Aor51HI 657 AGCGCT AGC^GCT
ApaLI 1844 GTGCAC G^TGCAC
AvaI 2301 CTCGAG C^[CT]CG[GA]G
AviII 4321 TGCGCA TGC^GCA
BalI 2781 TGGCCA TGG^CCA
BbrPI 20 CACGTG CAC^GTG
BbvCI 820 GCTGAGG CCTCAGC(-5/-2)
BfrI 1031 CTTAAG C^TTAAG
BglII 3647 AGATCT A^GATCT
BsgI 809 GTGCAG GTGCAG(16/14)
BsmBI 4697 CGTCTC CGTCTC(1/5)
BsoBI 2301 CTCGAG C^[CT]CG[GA]G
Bsp1407I 3780 TGTACA T^GTACA
BspLU11I 1098 ACATGT A^CATGT
BsrGI 3780 TGTACA T^GTACA
BstEII 826 GGTGACC G^GT[ACGT]ACC
BstPI 826 GGTGACC G^GT[ACGT]ACC
BstUI 1755 CGCG CG^CG
BstXI 4306 CCACTCATTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
DraII 4521 AGGACCT [GA]G^G[ACGT]CC[CT]
EaeI 2781 TGGCCA [CT]^GGCC[GA]
Eco47III 657 AGCGCT AGC^GCT
EcoO109I 4521 AGGACCT [GA]G^G[ACGT]CC[CT]
EcoO65I 826 GGTGACC G^GT[ACGT]ACC
FspI 4321 TGCGCA TGC^GCA
HpaI 2630 GTTAAC GTT^AAC
KspI 1754 CCGCGG CCGC^GG
MfeI 627 CAATTG C^AATTG
MluNI 2781 TGGCCA TGG^CCA
MscI 2781 TGGCCA TGG^CCA
MunI 627 CAATTG C^AATTG
MvnI 1755 CGCG CG^CG
NcoI 1180 CCATGG C^CATGG
NdeI 713 CATATG CA^TATG
PacI 2387 TTAATTAA TTAAT^TAA
PaeR7I 2301 CTCGAG C^TCGAG
PflMI 1240 CCATAATTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI 20 CACGTG CAC^GTG
PmlI 20 CACGTG CAC^GTG
PpuMI 4521 AGGACCT [GA]G^G[AT]CC[CT]
SacI 3531 GAGCTC GAGCT^C
SacII 1754 CCGCGG CCGC^GG
ScaI 1108 AGTACT AGT^ACT
SspBI 3780 TGTACA T^GTACA
Van91I 1240 CCATAATTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 3798 CCAGGAATCTTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 2301 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 16 enzymes) #
###################################
AccI 1463 GTCTAC GT^[AC][GT]AC
2254 GTAGAC
BanII 121 GGGCTC G[GA]GC[CT]^C
3531 GAGCTC
BciVI 1687 GGATAC GTATCC(6/5)
1927 GGATAC
BlpI 1801 GCTGAGC GC^T[ACGT]AGC
3920 GCTCAGC
Bpu1102I 1801 GCTGAGC GC^T[ACGT]AGC
3920 GCTCAGC
BsaAI 20 CACGTG [CT]AC^GT[GA]
4387 TACGTG
BspHI 466 TCATGA T^CATGA
2933 TCATGA
Cac8I 119 GCGGGC GC[ACGT]^[ACGT]GC
2065 GCCTGC
CelII 1801 GCTGAGC GC^T[ACGT]AGC
3920 GCTCAGC
EcoRV 272 GATATC GAT^ATC
282 GATATC
FauI 119 GCGGG CCCGC(4/6)
1756 GCGGG
HaeII 583 AGCGCC [GA]GCGC^[CT]
657 AGCGCT
PstI 2067 CTGCAG CTGCA^G
3466 CTGCAG
RcaI 466 TCATGA T^CATGA
2933 TCATGA
SapI 3236 GAAGAGC GCTCTTC(1/4)
4814 GCTCTTC
SpeI 35 ACTAGT A^CTAGT
943 ACTAGT
#########################################
# 3 OR MORE CUT ENZYMES ( 97 enzymes) #
#########################################
AciI AcsI AfaI AflIII AluI
AlwI ApoI AseI AsnI Asp700I
AspHI AvaII BamHI BbsI BbvI
BclI BfaI BmyI BpmI BpuAI
BsaJI BsiHKAI BsiYI BslI BsmAI
BsmFI BsmI Bsp1286I BsrDI BsrI
BssKI BstAPI BstF5I BstNI BstYI
CfoI Cfr13I DdeI DpnII DraI
DsaI EarI EcoRII EcoT14I EcoT22I
FbaI Fnu4HI FokI HaeIII HhaI
HinP1I HincII HindII HindIII HinfI
HphI ItaI Ksp632I MaeI MaeII
MaeIII MboI MboII MflI MnlI
MseI MslI MspA1I MvaI MwoI
NdeII NlaIII NlaIV NsiI NspI
PleI PshBI PvuII RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI SmlI
SspI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
XhoII XmnI