################################## # NO CUT ENZYMES ( 63 enzymes) # ################################## AatII AclI AgeI Aor51HI AscI AseI AsnI AviII AvrII BamHI BclI BlnI BsaBI BsiWI Bsp1407I BspDI BsrGI Bst1107I BstBI BstEII BstPI BstZ17I ClaI CpoI DraI DraIII DrdI Eco47III EcoO65I EcoRI EcoRV EcoT22I FbaI FseI FspI MamI MfeI MluI MunI NcoI NdeI NotI NruI NsiI NspV PacI PaeR7I PinAI PmeI PshBI Psp1406I PvuI RsrII SalI SfiI SfuI SgrAI SnaBI SpeI SspBI SwaI XbaI XhoI ################################### # ONE CUT ENZYMES ( 40 enzymes) # ################################### AccIII 1256 TCCGGA T^CCGGA AcsI 862 GAATTT [GA]^AATT[CT] AhdI 3763 GACCTGCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Alw44I 4822 GTGCAC G^TGCAC ApaLI 4822 GTGCAC G^TGCAC ApoI 862 GAATTT [GA]^AATT[CT] Asp700I 1114 GAAGGGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AspEI 3763 GACCTGCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC BbeI 2962 GGCGCC GGCGC^C BciVI 2234 GTATCC GTATCC(6/5) BglII 4208 AGATCT A^GATCT BseAI 1256 TCCGGA T^CCGGA BsmBI 3337 GAGACG CGTCTC(1/5) BspEI 1256 TCCGGA T^CCGGA BspHI 4389 TCATGA T^CATGA BspLU11I 4719 ACATGT A^CATGT BstAPI 690 GCAGCTCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC EagI 2681 CGGCCG C^GGCCG Eam1105I 3763 GACCTGCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EclXI 2681 CGGCCG C^GGCCG Eco52I 2681 CGGCCG C^GGCCG HpaI 4860 GTTAAC GTT^AAC KasI 2962 GGCGCC G^GCGCC KspI 449 CCGCGG CCGC^GG MroI 1256 TCCGGA T^CCGGA NarI 2962 GGCGCC GG^CGCC NheI 4583 GCTAGC G^CTAGC RcaI 4389 TCATGA T^CATGA SacII 449 CCGCGG CCGC^GG SbfI 2562 CCTGCAGG CCTGCA^GG ScaI 399 AGTACT AGT^ACT SexAI 2648 ACCTGGT A^CC[AT]GGT SfoI 2962 GGCGCC GGC^GCC SmaI 3121 CCCGGG CCC^GGG SphI 4142 GCATGC GCATG^C Sse8387I 2562 CCTGCAGG CCTGCA^GG SspI 714 AATATT AAT^ATT XmaCI 3121 CCCGGG C^CCGGG XmaI 3121 CCCGGG C^CCGGG XmnI 1114 GAAGGGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 15 enzymes) # ################################### AflII 631 CTTAAG C^TTAAG 640 CTTAAG AspI 4228 GACTGTGTC GAC[ACGT]^[ACGT][ACGT]GTC 5153 GACACCGTC BcgI 504 CGAGGTCTTTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) 3372 CGAGGCCCCTGC BfrI 631 CTTAAG C^TTAAG 640 CTTAAG BglI 109 GCCGAGCCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC 3479 GCCTGCGGGGC BsaI 62 GAGACC GGTCTC(1/5) 5039 GGTCTC BsaWI 1256 TCCGGA [AT]^CCGG[AT] 1331 ACCGGA BsiEI 77 CGACCG CG[GA][CT]^CG 2681 CGGCCG BsmI 4763 GAATGC GAATGC(1/-1) 4993 GAATGC HindIII 318 AAGCTT A^AGCTT 3754 AAGCTT NaeI 114 GCCGGC GCC^GGC 499 GCCGGC NgoMIV 114 GCCGGC G^CCGGC 499 GCCGGC PflFI 4228 GACTGTGTC GAC[ACGT]^[ACGT][ACGT]GTC 5153 GACACCGTC StuI 3308 AGGCCT AGG^CCT 4127 AGGCCT Tth111I 4228 GACTGTGTC GAC[ACGT]^[ACGT][ACGT]GTC 5153 GACACCGTC ######################################### # 3 OR MORE CUT ENZYMES ( 140 enzymes) # ######################################### Acc65I AccI AccII AciI AcyI AfaI AflIII AluI AlwI AlwNI AocI ApaI Asp718I AspHI AvaI AvaII BalI BanI BanII BbrPI BbsI BbvCI BbvI BcnI BfaI BlpI BmyI BpmI Bpu1102I BpuAI BsaAI BsaHI BsaJI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I BspMI BsrBI BsrDI BsrFI BsrI BssHII BssKI BssSI BstF5I BstNI BstUI BstXI BstYI Bsu36I Cac8I CelII CfoI Cfr10I Cfr13I DdeI DpnII DraII DsaI EaeI EarI Eco81I EcoNI EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeII HaeIII HapII HgaI HhaI Hin1I HinP1I HincII HindII HinfI HpaII HphI ItaI KpnI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MluNI MnlI MscI MseI MslI MspA1I MspI MvaI MvnI MwoI NciI NdeII NlaIII NlaIV NspI PflMI PleI PmaCI PmlI PpuMI PshAI PstI PvuII RsaI SacI SapI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I Van91I XcmI XhoII