##################################
# NO CUT ENZYMES (   63 enzymes) #
##################################

AatII       AclI        AgeI        Aor51HI     AscI        
AseI        AsnI        AviII       AvrII       BamHI       
BclI        BlnI        BsaBI       BsiWI       Bsp1407I    
BspDI       BsrGI       Bst1107I    BstBI       BstEII      
BstPI       BstZ17I     ClaI        CpoI        DraI        
DraIII      DrdI        Eco47III    EcoO65I     EcoRI       
EcoRV       EcoT22I     FbaI        FseI        FspI        
MamI        MfeI        MluI        MunI        NcoI        
NdeI        NotI        NruI        NsiI        NspV        
PacI        PaeR7I      PinAI       PmeI        PshBI       
Psp1406I    PvuI        RsrII       SalI        SfiI        
SfuI        SgrAI       SnaBI       SpeI        SspBI       
SwaI        XbaI        XhoI        


###################################
# ONE CUT ENZYMES (   40 enzymes) #
###################################

AccIII       1256  TCCGGA               T^CCGGA
AcsI          862  GAATTT               [GA]^AATT[CT]
AhdI         3763  GACCTGCAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I       4822  GTGCAC               G^TGCAC
ApaLI        4822  GTGCAC               G^TGCAC
ApoI          862  GAATTT               [GA]^AATT[CT]
Asp700I      1114  GAAGGGCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI        3763  GACCTGCAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BbeI         2962  GGCGCC               GGCGC^C
BciVI        2234  GTATCC               GTATCC(6/5)
BglII        4208  AGATCT               A^GATCT
BseAI        1256  TCCGGA               T^CCGGA
BsmBI        3337  GAGACG               CGTCTC(1/5)
BspEI        1256  TCCGGA               T^CCGGA
BspHI        4389  TCATGA               T^CATGA
BspLU11I     4719  ACATGT               A^CATGT
BstAPI        690  GCAGCTCCTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
EagI         2681  CGGCCG               C^GGCCG
Eam1105I     3763  GACCTGCAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI        2681  CGGCCG               C^GGCCG
Eco52I       2681  CGGCCG               C^GGCCG
HpaI         4860  GTTAAC               GTT^AAC
KasI         2962  GGCGCC               G^GCGCC
KspI          449  CCGCGG               CCGC^GG
MroI         1256  TCCGGA               T^CCGGA
NarI         2962  GGCGCC               GG^CGCC
NheI         4583  GCTAGC               G^CTAGC
RcaI         4389  TCATGA               T^CATGA
SacII         449  CCGCGG               CCGC^GG
SbfI         2562  CCTGCAGG             CCTGCA^GG
ScaI          399  AGTACT               AGT^ACT
SexAI        2648  ACCTGGT              A^CC[AT]GGT
SfoI         2962  GGCGCC               GGC^GCC
SmaI         3121  CCCGGG               CCC^GGG
SphI         4142  GCATGC               GCATG^C
Sse8387I     2562  CCTGCAGG             CCTGCA^GG
SspI          714  AATATT               AAT^ATT
XmaCI        3121  CCCGGG               C^CCGGG
XmaI         3121  CCCGGG               C^CCGGG
XmnI         1114  GAAGGGCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   15 enzymes) #
###################################

AflII         631  CTTAAG               C^TTAAG
              640  CTTAAG              
AspI         4228  GACTGTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
             5153  GACACCGTC           
BcgI          504  CGAGGTCTTTGC         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
             3372  CGAGGCCCCTGC        
BfrI          631  CTTAAG               C^TTAAG
              640  CTTAAG              
BglI          109  GCCGAGCCGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
             3479  GCCTGCGGGGC         
BsaI           62  GAGACC               GGTCTC(1/5)
             5039  GGTCTC              
BsaWI        1256  TCCGGA               [AT]^CCGG[AT]
             1331  ACCGGA              
BsiEI          77  CGACCG               CG[GA][CT]^CG
             2681  CGGCCG              
BsmI         4763  GAATGC               GAATGC(1/-1)
             4993  GAATGC              
HindIII       318  AAGCTT               A^AGCTT
             3754  AAGCTT              
NaeI          114  GCCGGC               GCC^GGC
              499  GCCGGC              
NgoMIV        114  GCCGGC               G^CCGGC
              499  GCCGGC              
PflFI        4228  GACTGTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
             5153  GACACCGTC           
StuI         3308  AGGCCT               AGG^CCT
             4127  AGGCCT              
Tth111I      4228  GACTGTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
             5153  GACACCGTC           


#########################################
# 3 OR MORE CUT ENZYMES (  140 enzymes) #
#########################################

Acc65I      AccI        AccII       AciI        AcyI        
AfaI        AflIII      AluI        AlwI        AlwNI       
AocI        ApaI        Asp718I     AspHI       AvaI        
AvaII       BalI        BanI        BanII       BbrPI       
BbsI        BbvCI       BbvI        BcnI        BfaI        
BlpI        BmyI        BpmI        Bpu1102I    BpuAI       
BsaAI       BsaHI       BsaJI       BseRI       BsgI        
BsiHKAI     BsiYI       BslI        BsmAI       BsmFI       
BsoBI       Bsp1286I    BspMI       BsrBI       BsrDI       
BsrFI       BsrI        BssHII      BssKI       BssSI       
BstF5I      BstNI       BstUI       BstXI       BstYI       
Bsu36I      Cac8I       CelII       CfoI        Cfr10I      
Cfr13I      DdeI        DpnII       DraII       DsaI        
EaeI        EarI        Eco81I      EcoNI       EcoO109I    
EcoRII      EcoT14I     FauI        Fnu4HI      FokI        
HaeII       HaeIII      HapII       HgaI        HhaI        
Hin1I       HinP1I      HincII      HindII      HinfI       
HpaII       HphI        ItaI        KpnI        Ksp632I     
MaeI        MaeII       MaeIII      MboI        MboII       
MflI        MluNI       MnlI        MscI        MseI        
MslI        MspA1I      MspI        MvaI        MvnI        
MwoI        NciI        NdeII       NlaIII      NlaIV       
NspI        PflMI       PleI        PmaCI       PmlI        
PpuMI       PshAI       PstI        PvuII       RsaI        
SacI        SapI        Sau3AI      Sau96I      ScrFI       
SfaNI       SfcI        SmlI        StyI        TaqI        
TfiI        Tru9I       TseI        Tsp45I      Tsp509I     
TspRI       TthHB8I     Van91I      XcmI        XhoII