##################################
# NO CUT ENZYMES (   63 enzymes) #
##################################

AatII       AccIII      AclI        AgeI        AscI        
AspI        AvrII       BalI        BamHI       BclI        
BglII       BlnI        BsaBI       BseAI       BsiWI       
Bsp1407I    BspDI       BspEI       BspHI       BspLU11I    
BsrGI       BssHII      Bst1107I    BstZ17I     ClaI        
EcoRI       EcoRV       EcoT22I     FbaI        HindIII     
HpaI        MamI        MfeI        MluI        MluNI       
MroI        MscI        MunI        NcoI        NdeI        
NheI        NruI        NsiI        PacI        PflFI       
PinAI       PmeI        Psp1406I    RcaI        SacI        
SapI        SbfI        ScaI        SexAI       SfiI        
SnaBI       SphI        Sse8387I    SspBI       StuI        
SwaI        Tth111I     XbaI        


###################################
# ONE CUT ENZYMES (   45 enzymes) #
###################################

AflII        2538  CTTAAG               C^TTAAG
AhdI         3234  GACAGCTTGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AseI          464  ATTAAT               AT^TAAT
AsnI          464  ATTAAT               AT^TAAT
Asp700I       909  GAAGCGATTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI        3234  GACAGCTTGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AviII         970  TGCGCA               TGC^GCA
BciVI         489  GTATCC               GTATCC(6/5)
BfrI         2538  CTTAAG               C^TTAAG
BglI          192  GCCACTGAGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaWI        1808  TCCGGT               [AT]^CCGG[AT]
BssSI        1793  CACGAG               CACGAG(-5/-1)
BstBI        2751  TTCGAA               TT^CGAA
BstEII        951  GGTGACC              G^GT[ACGT]ACC
BstPI         951  GGTGACC              G^GT[ACGT]ACC
DraIII       4239  CACCCCGTG            CAC[ACGT][ACGT][ACGT]^GTG
DrdI         1542  GACTCAGAGGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI         4948  CGGCCG               C^GGCCG
Eam1105I     3234  GACAGCTTGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI         4840  GAAGAG               CTCTTC(1/4)
EclXI        4948  CGGCCG               C^GGCCG
Eco52I       4948  CGGCCG               C^GGCCG
EcoO65I       951  GGTGACC              G^GT[ACGT]ACC
EcoT14I      3673  CCTTGG               C^C[AT][AT]GG
FseI          264  GGCCGGCC             GGCCGG^CC
FspI          970  TGCGCA               TGC^GCA
Ksp632I      4840  GAAGAG               CTCTTC(1/4)
NotI         4947  GCGGCCGC             GC^GGCCGC
NspV         2751  TTCGAA               TT^CGAA
PaeR7I        258  CTCGAG               C^TCGAG
PflMI        2921  CCACAGCCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PshAI        2862  GACATCAGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI         464  ATTAAT               AT^TAAT
PvuI         3950  CGATCG               CGAT^CG
SalI         1551  GTCGAC               G^TCGAC
SfuI         2751  TTCGAA               TT^CGAA
SgrAI         304  CGCCGGCG             C[GA]^CCGG[CT]G
SmaI         3653  CCCGGG               CCC^GGG
SpeI         3353  ACTAGT               A^CTAGT
StyI         3673  CCTTGG               C^C[AT][AT]GG
Van91I       2921  CCACAGCCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoI          258  CTCGAG               C^TCGAG
XmaCI        3653  CCCGGG               C^CCGGG
XmaI         3653  CCCGGG               C^CCGGG
XmnI          909  GAAGCGATTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   38 enzymes) #
###################################

Acc65I       2735  GGTACC               G^GTACC
             2777  GGTACC              
AccI         1551  GTCGAC               GT^[AC][GT]AC
             4383  GTCTAC              
AcsI          525  GAATTT               [GA]^AATT[CT]
             2896  AAATTC              
Alw44I       1500  GTGCAC               G^TGCAC
             4939  GTGCAC              
AocI         1883  CCTGAGG              CC^T[ACGT]AGG
             4474  CCTCAGG             
Aor51HI      2000  AGCGCT               AGC^GCT
             3314  AGCGCT              
ApaLI        1500  GTGCAC               G^TGCAC
             4939  GTGCAC              
ApoI          525  GAATTT               [GA]^AATT[CT]
             2896  AAATTC              
Asp718I      2735  GGTACC               G^GTACC
             2777  GGTACC              
BbeI          401  GGCGCC               GGCGC^C
             4759  GGCGCC              
BbrPI        2160  CACGTG               CAC^GTG
             2819  CACGTG              
BcgI         1634  CGACCACTTTGC         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
             3992  CGAACTCCTTGC        
BlpI         1734  GCTAAGC              GC^T[ACGT]AGC
             4601  GCTCAGC             
Bpu1102I     1734  GCTAAGC              GC^T[ACGT]AGC
             4601  GCTCAGC             
BsaAI        2160  CACGTG               [CT]AC^GT[GA]
             2819  CACGTG              
BsiEI        3950  CGATCG               CG[GA][CT]^CG
             4948  CGGCCG              
BsmBI        3561  GAGACG               CGTCTC(1/5)
             4847  GAGACG              
BsrDI        2710  CATTGC               GCAATG(2/0)
             4630  CATTGC              
BstXI        3805  CCAGTGACCTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             4411  CCAAAGAGCTGG        
Bsu36I       1883  CCTGAGG              CC^T[ACGT]AGG
             4474  CCTCAGG             
CelII        1734  GCTAAGC              GC^T[ACGT]AGC
             4601  GCTCAGC             
CpoI          128  CGGACCG              CG^G[AT]CCG
              140  CGGACCG             
DraI         2283  TTTAAA               TTT^AAA
             3140  TTTAAA              
Eco47III     2000  AGCGCT               AGC^GCT
             3314  AGCGCT              
Eco81I       1883  CCTGAGG              CC^T[ACGT]AGG
             4474  CCTCAGG             
EcoNI        3224  CCTCCTGGAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
             4121  CCTCTCAGAGG         
HincII       1551  GTCGAC               GT[CT]^[GA]AC
             4625  GTCAAC              
HindII       1551  GTCGAC               GT[CT]^[GA]AC
             4625  GTCAAC              
KasI          401  GGCGCC               G^GCGCC
             4759  GGCGCC              
KpnI         2735  GGTACC               GGTAC^C
             2777  GGTACC              
MslI         2514  CACATAAATG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
             3545  CACAGAAGTG          
NarI          401  GGCGCC               GG^CGCC
             4759  GGCGCC              
NspI          988  ACATGC               [GA]CATG^[CT]
             2629  GCATGT              
PmaCI        2160  CACGTG               CAC^GTG
             2819  CACGTG              
PmlI         2160  CACGTG               CAC^GTG
             2819  CACGTG              
PvuII        1413  CAGCTG               CAG^CTG
             3571  CAGCTG              
RsrII         128  CGGACCG              CG^G[AT]CCG
              140  CGGACCG             
SfoI          401  GGCGCC               GGC^GCC
             4759  GGCGCC              


#########################################
# 3 OR MORE CUT ENZYMES (  112 enzymes) #
#########################################

AccII       AciI        AcyI        AfaI        AflIII      
AluI        AlwI        AlwNI       ApaI        AspHI       
AvaI        AvaII       BanI        BanII       BbsI        
BbvCI       BbvI        BcnI        BfaI        BmyI        
BpmI        BpuAI       BsaHI       BsaI        BsaJI       
BseRI       BsgI        BsiHKAI     BsiYI       BslI        
BsmAI       BsmFI       BsmI        BsoBI       Bsp1286I    
BspMI       BsrBI       BsrFI       BsrI        BssKI       
BstAPI      BstF5I      BstNI       BstUI       BstYI       
Cac8I       CfoI        Cfr10I      Cfr13I      DdeI        
DpnII       DraII       DsaI        EaeI        EcoO109I    
EcoRII      FauI        Fnu4HI      FokI        HaeII       
HaeIII      HapII       HgaI        HhaI        Hin1I       
HinP1I      HinfI       HpaII       HphI        ItaI        
KspI        MaeI        MaeII       MaeIII      MboI        
MboII       MflI        MnlI        MseI        MspA1I      
MspI        MvaI        MvnI        MwoI        NaeI        
NciI        NdeII       NgoMIV      NlaIII      NlaIV       
PleI        PpuMI       PstI        RsaI        SacII       
Sau3AI      Sau96I      ScrFI       SfaNI       SfcI        
SmlI        SspI        TaqI        TfiI        Tru9I       
TseI        Tsp45I      Tsp509I     TspRI       TthHB8I     
XcmI        XhoII