################################## # NO CUT ENZYMES ( 63 enzymes) # ################################## AatII AccIII AclI AgeI AscI AspI AvrII BalI BamHI BclI BglII BlnI BsaBI BseAI BsiWI Bsp1407I BspDI BspEI BspHI BspLU11I BsrGI BssHII Bst1107I BstZ17I ClaI EcoRI EcoRV EcoT22I FbaI HindIII HpaI MamI MfeI MluI MluNI MroI MscI MunI NcoI NdeI NheI NruI NsiI PacI PflFI PinAI PmeI Psp1406I RcaI SacI SapI SbfI ScaI SexAI SfiI SnaBI SphI Sse8387I SspBI StuI SwaI Tth111I XbaI ################################### # ONE CUT ENZYMES ( 45 enzymes) # ################################### AflII 2538 CTTAAG C^TTAAG AhdI 3234 GACAGCTTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AseI 464 ATTAAT AT^TAAT AsnI 464 ATTAAT AT^TAAT Asp700I 909 GAAGCGATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AspEI 3234 GACAGCTTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AviII 970 TGCGCA TGC^GCA BciVI 489 GTATCC GTATCC(6/5) BfrI 2538 CTTAAG C^TTAAG BglI 192 GCCACTGAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BsaWI 1808 TCCGGT [AT]^CCGG[AT] BssSI 1793 CACGAG CACGAG(-5/-1) BstBI 2751 TTCGAA TT^CGAA BstEII 951 GGTGACC G^GT[ACGT]ACC BstPI 951 GGTGACC G^GT[ACGT]ACC DraIII 4239 CACCCCGTG CAC[ACGT][ACGT][ACGT]^GTG DrdI 1542 GACTCAGAGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EagI 4948 CGGCCG C^GGCCG Eam1105I 3234 GACAGCTTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EarI 4840 GAAGAG CTCTTC(1/4) EclXI 4948 CGGCCG C^GGCCG Eco52I 4948 CGGCCG C^GGCCG EcoO65I 951 GGTGACC G^GT[ACGT]ACC EcoT14I 3673 CCTTGG C^C[AT][AT]GG FseI 264 GGCCGGCC GGCCGG^CC FspI 970 TGCGCA TGC^GCA Ksp632I 4840 GAAGAG CTCTTC(1/4) NotI 4947 GCGGCCGC GC^GGCCGC NspV 2751 TTCGAA TT^CGAA PaeR7I 258 CTCGAG C^TCGAG PflMI 2921 CCACAGCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PshAI 2862 GACATCAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC PshBI 464 ATTAAT AT^TAAT PvuI 3950 CGATCG CGAT^CG SalI 1551 GTCGAC G^TCGAC SfuI 2751 TTCGAA TT^CGAA SgrAI 304 CGCCGGCG C[GA]^CCGG[CT]G SmaI 3653 CCCGGG CCC^GGG SpeI 3353 ACTAGT A^CTAGT StyI 3673 CCTTGG C^C[AT][AT]GG Van91I 2921 CCACAGCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XhoI 258 CTCGAG C^TCGAG XmaCI 3653 CCCGGG C^CCGGG XmaI 3653 CCCGGG C^CCGGG XmnI 909 GAAGCGATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 38 enzymes) # ################################### Acc65I 2735 GGTACC G^GTACC 2777 GGTACC AccI 1551 GTCGAC GT^[AC][GT]AC 4383 GTCTAC AcsI 525 GAATTT [GA]^AATT[CT] 2896 AAATTC Alw44I 1500 GTGCAC G^TGCAC 4939 GTGCAC AocI 1883 CCTGAGG CC^T[ACGT]AGG 4474 CCTCAGG Aor51HI 2000 AGCGCT AGC^GCT 3314 AGCGCT ApaLI 1500 GTGCAC G^TGCAC 4939 GTGCAC ApoI 525 GAATTT [GA]^AATT[CT] 2896 AAATTC Asp718I 2735 GGTACC G^GTACC 2777 GGTACC BbeI 401 GGCGCC GGCGC^C 4759 GGCGCC BbrPI 2160 CACGTG CAC^GTG 2819 CACGTG BcgI 1634 CGACCACTTTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) 3992 CGAACTCCTTGC BlpI 1734 GCTAAGC GC^T[ACGT]AGC 4601 GCTCAGC Bpu1102I 1734 GCTAAGC GC^T[ACGT]AGC 4601 GCTCAGC BsaAI 2160 CACGTG [CT]AC^GT[GA] 2819 CACGTG BsiEI 3950 CGATCG CG[GA][CT]^CG 4948 CGGCCG BsmBI 3561 GAGACG CGTCTC(1/5) 4847 GAGACG BsrDI 2710 CATTGC GCAATG(2/0) 4630 CATTGC BstXI 3805 CCAGTGACCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 4411 CCAAAGAGCTGG Bsu36I 1883 CCTGAGG CC^T[ACGT]AGG 4474 CCTCAGG CelII 1734 GCTAAGC GC^T[ACGT]AGC 4601 GCTCAGC CpoI 128 CGGACCG CG^G[AT]CCG 140 CGGACCG DraI 2283 TTTAAA TTT^AAA 3140 TTTAAA Eco47III 2000 AGCGCT AGC^GCT 3314 AGCGCT Eco81I 1883 CCTGAGG CC^T[ACGT]AGG 4474 CCTCAGG EcoNI 3224 CCTCCTGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG 4121 CCTCTCAGAGG HincII 1551 GTCGAC GT[CT]^[GA]AC 4625 GTCAAC HindII 1551 GTCGAC GT[CT]^[GA]AC 4625 GTCAAC KasI 401 GGCGCC G^GCGCC 4759 GGCGCC KpnI 2735 GGTACC GGTAC^C 2777 GGTACC MslI 2514 CACATAAATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 3545 CACAGAAGTG NarI 401 GGCGCC GG^CGCC 4759 GGCGCC NspI 988 ACATGC [GA]CATG^[CT] 2629 GCATGT PmaCI 2160 CACGTG CAC^GTG 2819 CACGTG PmlI 2160 CACGTG CAC^GTG 2819 CACGTG PvuII 1413 CAGCTG CAG^CTG 3571 CAGCTG RsrII 128 CGGACCG CG^G[AT]CCG 140 CGGACCG SfoI 401 GGCGCC GGC^GCC 4759 GGCGCC ######################################### # 3 OR MORE CUT ENZYMES ( 112 enzymes) # ######################################### AccII AciI AcyI AfaI AflIII AluI AlwI AlwNI ApaI AspHI AvaI AvaII BanI BanII BbsI BbvCI BbvI BcnI BfaI BmyI BpmI BpuAI BsaHI BsaI BsaJI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI BsmI BsoBI Bsp1286I BspMI BsrBI BsrFI BsrI BssKI BstAPI BstF5I BstNI BstUI BstYI Cac8I CfoI Cfr10I Cfr13I DdeI DpnII DraII DsaI EaeI EcoO109I EcoRII FauI Fnu4HI FokI HaeII HaeIII HapII HgaI HhaI Hin1I HinP1I HinfI HpaII HphI ItaI KspI MaeI MaeII MaeIII MboI MboII MflI MnlI MseI MspA1I MspI MvaI MvnI MwoI NaeI NciI NdeII NgoMIV NlaIII NlaIV PleI PpuMI PstI RsaI SacII Sau3AI Sau96I ScrFI SfaNI SfcI SmlI SspI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I XcmI XhoII