##################################
# NO CUT ENZYMES ( 78 enzymes) #
##################################
Acc65I AccI AccIII AhdI Aor51HI
AscI Asp718I AspEI AspI AviII
AvrII BamHI BbeI BbvCI BciVI
BlnI BlpI Bpu1102I BseAI BsiWI
BsmI BspEI BspHI BsrBI BssHII
Bst1107I BstAPI BstZ17I CelII CpoI
DrdI EagI Eam1105I EarI EclXI
Eco47III Eco52I EcoRI EcoRV FseI
FspI HaeII HpaI KasI KpnI
Ksp632I KspI MluI MroI NaeI
NarI NdeI NgoMIV NotI NruI
PacI PaeR7I PflFI PmeI PshAI
PvuI RcaI RsrII SacII SalI
SapI SbfI ScaI SfiI SfoI
SgrAI SphI Sse8387I StuI SwaI
Tth111I XcmI XhoI
###################################
# ONE CUT ENZYMES ( 41 enzymes) #
###################################
AatII 1077 GACGTC GACGT^C
AclI 407 AACGTT AA^CGTT
AgeI 3045 ACCGGT A^CCGGT
Alw44I 3999 GTGCAC G^TGCAC
AocI 2282 CCTGAGG CC^T[ACGT]AGG
ApaI 3207 GGGCCC GGGCC^C
ApaLI 3999 GTGCAC G^TGCAC
AseI 1699 ATTAAT AT^TAAT
AsnI 1699 ATTAAT AT^TAAT
Asp700I 1224 GAATGTTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BclI 3938 TGATCA T^GATCA
BglII 3671 AGATCT A^GATCT
BseRI 1164 CTCCTC GAGGAG(10/8)
BsiEI 28 CGACCG CG[GA][CT]^CG
BspDI 4371 ATCGAT AT^CGAT
BspLU11I 266 ACATGT A^CATGT
BstBI 3160 TTCGAA TT^CGAA
Bsu36I 2282 CCTGAGG CC^T[ACGT]AGG
ClaI 4371 ATCGAT AT^CGAT
DraI 3703 TTTAAA TTT^AAA
Eco81I 2282 CCTGAGG CC^T[ACGT]AGG
EcoNI 3424 CCTGATGCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoT22I 4332 ATGCAT ATGCA^T
FauI 2772 CCCGC CCCGC(4/6)
FbaI 3938 TGATCA T^GATCA
MfeI 3490 CAATTG C^AATTG
MunI 3490 CAATTG C^AATTG
NcoI 4349 CCATGG C^CATGG
NheI 2295 GCTAGC G^CTAGC
NsiI 4332 ATGCAT ATGCA^T
NspV 3160 TTCGAA TT^CGAA
PinAI 3045 ACCGGT A^CCGGT
PshBI 1699 ATTAAT AT^TAAT
Psp1406I 407 AACGTT AA^CGTT
SfuI 3160 TTCGAA TT^CGAA
SmaI 3204 CCCGGG CCC^GGG
SpeI 865 ACTAGT A^CTAGT
XbaI 3175 TCTAGA T^CTAGA
XmaCI 3204 CCCGGG C^CCGGG
XmaI 3204 CCCGGG C^CCGGG
XmnI 1224 GAATGTTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 29 enzymes) #
###################################
AflII 3661 CTTAAG C^TTAAG
4292 CTTAAG
AvaI 658 CTCGGG C^[CT]CG[GA]G
3204 CCCGGG
BalI 635 TGGCCA TGG^CCA
3724 TGGCCA
BcgI 101 CGAGCTTATTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
160 GCAAGTTGTTCG
BfrI 3661 CTTAAG C^TTAAG
4292 CTTAAG
BsaBI 2315 GATTCTAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
3379 GATTAGAATC
BsaI 2287 GGTCTC GGTCTC(1/5)
2569 GGTCTC
BsaWI 1049 ACCGGA [AT]^CCGG[AT]
3045 ACCGGT
BsmBI 1075 GAGACG CGTCTC(1/5)
2545 GAGACG
BsoBI 658 CTCGGG C^[CT]CG[GA]G
3204 CCCGGG
Bsp1407I 1785 TGTACA T^GTACA
4246 TGTACA
BspMI 3565 ACCTGC ACCTGC(4/8)
3055 GCAGGT
BsrGI 1785 TGTACA T^GTACA
4246 TGTACA
BssSI 586 CTCGTG CACGAG(-5/-1)
1153 CTCGTG
BstEII 1158 GGTGACC G^GT[ACGT]ACC
2168 GGTAACC
BstPI 1158 GGTGACC G^GT[ACGT]ACC
2168 GGTAACC
BstXI 3563 CCACCTGCTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
4179 CCATGCTGTTGG
EcoO65I 1158 GGTGACC G^GT[ACGT]ACC
2168 GGTAACC
HindIII 3698 AAGCTT A^AGCTT
4458 AAGCTT
MamI 2315 GATTCTAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
3379 GATTAGAATC
MluNI 635 TGGCCA TGG^CCA
3724 TGGCCA
MscI 635 TGGCCA TGG^CCA
3724 TGGCCA
NspI 266 ACATGT [GA]CATG^[CT]
3817 GCATGT
PflMI 984 CCAGGGCGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1583 CCATTGGTTGG
PstI 2703 CTGCAG CTGCA^G
3927 CTGCAG
SexAI 2825 ACCTGGT A^CC[AT]GGT
3362 ACCTGGT
SnaBI 380 TACGTA TAC^GTA
2013 TACGTA
SspBI 1785 TGTACA T^GTACA
4246 TGTACA
Van91I 984 CCAGGGCGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1583 CCATTGGTTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 110 enzymes) #
#########################################
AccII AciI AcsI AcyI AfaI
AflIII AluI AlwI AlwNI ApoI
AspHI AvaII BanI BanII BbrPI
BbsI BbvI BcnI BfaI BglI
BmyI BpmI BpuAI BsaAI BsaHI
BsaJI BsgI BsiHKAI BsiYI BslI
BsmAI BsmFI Bsp1286I BsrDI BsrFI
BsrI BssKI BstF5I BstNI BstUI
BstYI Cac8I CfoI Cfr10I Cfr13I
DdeI DpnII DraII DraIII DsaI
EaeI EcoO109I EcoRII EcoT14I Fnu4HI
FokI HaeIII HapII HgaI HhaI
Hin1I HinP1I HincII HindII HinfI
HpaII HphI ItaI MaeI MaeII
MaeIII MboI MboII MflI MnlI
MseI MslI MspA1I MspI MvaI
MvnI MwoI NciI NdeII NlaIII
NlaIV PleI PmaCI PmlI PpuMI
PvuII RsaI SacI Sau3AI Sau96I
ScrFI SfaNI SfcI SmlI SspI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I XhoII