##################################
# NO CUT ENZYMES ( 80 enzymes) #
##################################
AatII Acc65I AccII AclI AgeI
AhdI Alw44I Aor51HI ApaLI AscI
Asp718I AspEI AviII AvrII BamHI
BbeI BcgI BciVI BlnI BsaBI
BsiEI BsiWI Bsp1407I BspDI BspLU11I
BsrFI BsrGI BssHII Bst1107I BstBI
BstUI BstZ17I Cfr10I ClaI CpoI
DrdI EagI Eam1105I EclXI Eco47III
Eco52I EcoRI FseI FspI HindIII
HpaI KasI KpnI KspI MamI
MluI MvnI NaeI NarI NdeI
NgoMIV NotI NruI NspV PacI
PaeR7I PinAI PmeI Psp1406I PvuI
RsrII SacII SapI SbfI SexAI
SfiI SfoI SfuI SgrAI SpeI
SphI Sse8387I SspBI SwaI XhoI
###################################
# ONE CUT ENZYMES ( 39 enzymes) #
###################################
AccIII 1311 TCCGGA T^CCGGA
AcyI 3487 GACGCC G[GA]^CG[CT]C
AflIII 2338 ACGTGT A^C[GA][CT]GT
AocI 815 CCTTAGG CC^T[ACGT]AGG
BglI 1588 GCCAGAAGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 4308 AGATCT A^GATCT
BlpI 1861 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 1861 GCTCAGC GC^T[ACGT]AGC
BsaHI 3487 GACGCC G[GA]^CG[CT]C
BseAI 1311 TCCGGA T^CCGGA
BseRI 4688 CTCCTC GAGGAG(10/8)
BspEI 1311 TCCGGA T^CCGGA
BspHI 1554 TCATGA T^CATGA
BsrBI 684 GAGCGG CCGCTC(-3/-3)
BsrDI 614 GCAATG GCAATG(2/0)
BstAPI 4100 GCAGAGATTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 4572 GGTTACC G^GT[ACGT]ACC
BstPI 4572 GGTTACC G^GT[ACGT]ACC
Bsu36I 815 CCTTAGG CC^T[ACGT]AGG
CelII 1861 GCTCAGC GC^T[ACGT]AGC
DsaI 5209 CCATGG C^C[GA][CT]GG
Eco81I 815 CCTTAGG CC^T[ACGT]AGG
EcoNI 1672 CCTGATTCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 4572 GGTTACC G^GT[ACGT]ACC
EcoRV 1732 GATATC GAT^ATC
HaeII 2816 AGCGCC [GA]GCGC^[CT]
HgaI 3487 GACGC GACGC(5/10)
Hin1I 3487 GACGCC G[GA]^CG[CT]C
MfeI 1406 CAATTG C^AATTG
MroI 1311 TCCGGA T^CCGGA
MunI 1406 CAATTG C^AATTG
NcoI 5209 CCATGG C^CATGG
NheI 3270 GCTAGC G^CTAGC
NspI 4029 GCATGT [GA]CATG^[CT]
PshAI 3487 GACGCCAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
RcaI 1554 TCATGA T^CATGA
SalI 4146 GTCGAC G^TCGAC
SnaBI 4235 TACGTA TAC^GTA
XcmI 1716 CCAGGTGGCTTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 24 enzymes) #
###################################
AccI 4146 GTCGAC GT^[AC][GT]AC
4600 GTAGAC
AflII 508 CTTAAG C^TTAAG
5197 CTTAAG
ApaI 673 GGGCCC GGGCC^C
5050 GGGCCC
AseI 1467 ATTAAT AT^TAAT
2740 ATTAAT
AsnI 1467 ATTAAT AT^TAAT
2740 ATTAAT
BbvCI 3452 GCTGAGG CCTCAGC(-5/-2)
4068 GCTGAGG
BclI 1551 TGATCA T^GATCA
2570 TGATCA
BfrI 508 CTTAAG C^TTAAG
5197 CTTAAG
BsaWI 1311 TCCGGA [AT]^CCGG[AT]
1891 ACCGGA
BsmBI 3998 CGTCTC CGTCTC(1/5)
4164 CGTCTC
BssSI 3951 CACGAG CACGAG(-5/-1)
1144 CTCGTG
BstXI 3920 CCAGCACTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
4053 CCACCTACTTGG
DraIII 652 CACCACGTG CAC[ACGT][ACGT][ACGT]^GTG
4978 CACATTGTG
EcoT22I 491 ATGCAT ATGCA^T
4227 ATGCAT
FbaI 1551 TGATCA T^GATCA
2570 TGATCA
NsiI 491 ATGCAT ATGCA^T
4227 ATGCAT
PpuMI 503 AGGACCT [GA]G^G[AT]CC[CT]
5215 AGGTCCC
PshBI 1467 ATTAAT AT^TAAT
2740 ATTAAT
PstI 1069 CTGCAG CTGCA^G
2461 CTGCAG
SacI 559 GAGCTC GAGCT^C
1198 GAGCTC
SmaI 1059 CCCGGG CCC^GGG
2606 CCCGGG
XbaI 3107 TCTAGA T^CTAGA
3848 TCTAGA
XmaCI 1059 CCCGGG C^CCGGG
2606 CCCGGG
XmaI 1059 CCCGGG C^CCGGG
2606 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 115 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
AlwNI ApoI Asp700I AspHI AspI
AvaI AvaII BalI BanI BanII
BbrPI BbsI BbvI BcnI BfaI
BmyI BpmI BpuAI BsaAI BsaI
BsaJI BsgI BsiHKAI BsiYI BslI
BsmAI BsmFI BsmI BsoBI Bsp1286I
BspMI BsrI BssKI BstF5I BstNI
BstYI Cac8I CfoI Cfr13I DdeI
DpnII DraI DraII EaeI EarI
EcoO109I EcoRII EcoT14I FauI Fnu4HI
FokI HaeIII HapII HhaI HinP1I
HincII HindII HinfI HpaII HphI
ItaI Ksp632I MaeI MaeII MaeIII
MboI MboII MflI MluNI MnlI
MscI MseI MslI MspA1I MspI
MvaI MwoI NciI NdeII NlaIII
NlaIV PflFI PflMI PleI PmaCI
PmlI PvuII RsaI Sau3AI Sau96I
ScaI ScrFI SfaNI SfcI SmlI
SspI StuI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
Tth111I TthHB8I Van91I XhoII XmnI