##################################
# NO CUT ENZYMES ( 72 enzymes) #
##################################
AatII Acc65I AccIII AgeI ApaI
AscI Asp718I AspI AvrII BamHI
BbrPI BlnI BlpI Bpu1102I BsaBI
BsaWI BseAI BsgI BsiEI BsiWI
BsmBI BsmFI BspEI BsrBI BssHII
BssSI Bst1107I BstBI BstEII BstPI
BstZ17I CelII CpoI DraIII DrdI
EagI EclXI Eco52I EcoO65I KpnI
KspI MamI MluI MroI NheI
NotI NruI NspV PacI PflFI
PflMI PinAI PmaCI PmeI PmlI
PshAI PvuI RsrII SacII SalI
SbfI SexAI SfuI SmaI SnaBI
Sse8387I SwaI Tth111I Van91I XcmI
XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 49 enzymes) #
###################################
AclI 4148 AACGTT AA^CGTT
AflIII 2709 ACATGT A^C[GA][CT]GT
Alw44I 2100 GTGCAC G^TGCAC
AocI 3748 CCTCAGG CC^T[ACGT]AGG
Aor51HI 460 AGCGCT AGC^GCT
ApaLI 2100 GTGCAC G^TGCAC
AviII 613 TGCGCA TGC^GCA
BalI 781 TGGCCA TGG^CCA
BbvCI 3633 CCTCAGC CCTCAGC(-5/-2)
BcgI 513 GCACCTGCCTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI 4370 TGATCA T^GATCA
BglII 2028 AGATCT A^GATCT
BsaAI 1368 TACGTG [CT]AC^GT[GA]
BspDI 618 ATCGAT AT^CGAT
BspLU11I 2709 ACATGT A^CATGT
BstF5I 626 GGATG GGATG(2/0)
BstXI 3776 CCAAAGTGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 3748 CCTCAGG CC^T[ACGT]AGG
ClaI 618 ATCGAT AT^CGAT
DraII 1799 GGGTCCT [GA]G^G[ACGT]CC[CT]
DsaI 943 CCATGG C^C[GA][CT]GG
Eco47III 460 AGCGCT AGC^GCT
Eco81I 3748 CCTCAGG CC^T[ACGT]AGG
EcoO109I 1799 GGGTCCT [GA]G^G[ACGT]CC[CT]
EcoRV 4748 GATATC GAT^ATC
FbaI 4370 TGATCA T^GATCA
FokI 626 GGATG GGATG(9/13)
FseI 331 GGCCGGCC GGCCGG^CC
FspI 613 TGCGCA TGC^GCA
HincII 2783 GTTAAC GT[CT]^[GA]AC
HindII 2783 GTTAAC GT[CT]^[GA]AC
HindIII 4764 AAGCTT A^AGCTT
HpaI 2783 GTTAAC GTT^AAC
MfeI 2575 CAATTG C^AATTG
MluNI 781 TGGCCA TGG^CCA
MscI 781 TGGCCA TGG^CCA
MunI 2575 CAATTG C^AATTG
NcoI 943 CCATGG C^CATGG
PaeR7I 1414 CTCGAG C^TCGAG
PpuMI 1799 GGGTCCT [GA]G^G[AT]CC[CT]
Psp1406I 4148 AACGTT AA^CGTT
PvuII 1135 CAGCTG CAG^CTG
SapI 4650 GCTCTTC GCTCTTC(1/4)
SfiI 900 GGCCTTTCAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SgrAI 538 CGCCGGTG C[GA]^CCGG[CT]G
SpeI 849 ACTAGT A^CTAGT
SphI 3663 GCATGC GCATG^C
XbaI 803 TCTAGA T^CTAGA
XhoI 1414 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 25 enzymes) #
###################################
AccI 1498 GTAGAC GT^[AC][GT]AC
2776 GTAGAC
AflII 2449 CTTAAG C^TTAAG
4753 CTTAAG
AhdI 4777 GACAGTCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
4974 GACTCTGAGTC
AseI 2990 ATTAAT AT^TAAT
4552 ATTAAT
AsnI 2990 ATTAAT AT^TAAT
4552 ATTAAT
AspEI 4777 GACAGTCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
4974 GACTCTGAGTC
BbeI 264 GGCGCC GGCGC^C
536 GGCGCC
BciVI 1752 GGATAC GTATCC(6/5)
3989 GTATCC
BfrI 2449 CTTAAG C^TTAAG
4753 CTTAAG
BsaI 103 GAGACC GGTCTC(1/5)
4001 GGTCTC
BsmI 1322 GAATGC GAATGC(1/-1)
2435 GAATGC
EaeI 43 TGGCCG [CT]^GGCC[GA]
781 TGGCCA
Eam1105I 4777 GACAGTCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
4974 GACTCTGAGTC
EcoNI 41 CCTGGCCGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
1245 CCTGCCAGAGG
KasI 264 GGCGCC G^GCGCC
536 GGCGCC
MslI 2093 CATATTTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
4641 CATTAGTGTG
MspA1I 387 CCGCTG C[AC]G^C[GT]G
1135 CAGCTG
NaeI 69 GCCGGC GCC^GGC
332 GCCGGC
NarI 264 GGCGCC GG^CGCC
536 GGCGCC
NdeI 3842 CATATG CA^TATG
3969 CATATG
NgoMIV 69 GCCGGC G^CCGGC
332 GCCGGC
NspI 2709 ACATGT [GA]CATG^[CT]
3663 GCATGC
PshBI 2990 ATTAAT AT^TAAT
4552 ATTAAT
ScaI 1562 AGTACT AGT^ACT
4733 AGTACT
SfoI 264 GGCGCC GGC^GCC
536 GGCGCC
#########################################
# 3 OR MORE CUT ENZYMES ( 112 enzymes) #
#########################################
AccII AciI AcsI AcyI AfaI
AluI AlwI AlwNI ApoI Asp700I
AspHI AvaI AvaII BanI BanII
BbsI BbvI BcnI BfaI BglI
BmyI BpmI BpuAI BsaHI BsaJI
BseRI BsiHKAI BsiYI BslI BsmAI
BsoBI Bsp1286I Bsp1407I BspHI BspMI
BsrDI BsrFI BsrGI BsrI BssKI
BstAPI BstNI BstUI BstYI Cac8I
CfoI Cfr10I Cfr13I DdeI DpnII
DraI EarI EcoRI EcoRII EcoT14I
EcoT22I FauI Fnu4HI HaeII HaeIII
HapII HgaI HhaI Hin1I HinP1I
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeII MaeIII MboI MboII
MflI MnlI MseI MspI MvaI
MvnI MwoI NciI NdeII NlaIII
NlaIV NsiI PleI PstI RcaI
RsaI SacI Sau3AI Sau96I ScrFI
SfaNI SfcI SmlI SspBI SspI
StuI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
XhoII XmnI