##################################
# NO CUT ENZYMES (   59 enzymes) #
##################################

AatII       AclI        AflII       AhdI        Aor51HI     
AscI        AseI        AsnI        Asp700I     AspEI       
AviII       BbvCI       BfrI        BglII       BsaBI       
BsiWI       Bsp1407I    BspLU11I    BsrGI       Bst1107I    
BstBI       BstZ17I     CpoI        DraI        DrdI        
Eam1105I    Eco47III    FspI        HindIII     HpaI        
MamI        MfeI        MluI        MunI        NdeI        
NheI        NotI        NruI        NspV        PacI        
PaeR7I      PmeI        PshAI       PshBI       Psp1406I    
PvuI        RsrII       SalI        ScaI        SfuI        
SgrAI       SnaBI       SpeI        SspBI       SspI        
SwaI        XbaI        XhoI        XmnI        


###################################
# ONE CUT ENZYMES (   33 enzymes) #
###################################

Acc65I       3209  GGTACC               G^GTACC
AccIII       1445  TCCGGA               T^CCGGA
AflIII       4851  ACACGT               A^C[GA][CT]GT
AgeI         4592  ACCGGT               A^CCGGT
Alw44I       3201  GTGCAC               G^TGCAC
AocI          220  CCTCAGG              CC^T[ACGT]AGG
ApaLI        3201  GTGCAC               G^TGCAC
Asp718I      3209  GGTACC               G^GTACC
BcgI         2791  GCAAGAAGTTCG         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI         639  GGATAC               GTATCC(6/5)
BclI         4776  TGATCA               T^GATCA
BseAI        1445  TCCGGA               T^CCGGA
BsmI         4292  GCATTC               GAATGC(1/-1)
BspDI        4011  ATCGAT               AT^CGAT
BspEI        1445  TCCGGA               T^CCGGA
BsrBI        4734  GAGCGG               CCGCTC(-3/-3)
BstEII       1499  GGTGACC              G^GT[ACGT]ACC
BstPI        1499  GGTGACC              G^GT[ACGT]ACC
Bsu36I        220  CCTCAGG              CC^T[ACGT]AGG
ClaI         4011  ATCGAT               AT^CGAT
Eco81I        220  CCTCAGG              CC^T[ACGT]AGG
EcoO65I      1499  GGTGACC              G^GT[ACGT]ACC
EcoRI         516  GAATTC               G^AATTC
EcoRV        2049  GATATC               GAT^ATC
EcoT22I      4872  ATGCAT               ATGCA^T
FbaI         4776  TGATCA               T^GATCA
FseI         2498  GGCCGGCC             GGCCGG^CC
KpnI         3209  GGTACC               GGTAC^C
MroI         1445  TCCGGA               T^CCGGA
NsiI         4872  ATGCAT               ATGCA^T
PinAI        4592  ACCGGT               A^CCGGT
SfiI         2468  GGCCGCGCTGGCC        GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SphI         3505  GCATGC               GCATG^C


###################################
# TWO CUT ENZYMES (   21 enzymes) #
###################################

AccI         1398  GTCTAC               GT^[AC][GT]AC
             4947  GTCTAC              
AcsI          516  GAATTC               [GA]^AATT[CT]
             4526  AAATTC              
ApoI          516  GAATTC               [GA]^AATT[CT]
             4526  AAATTC              
AspI         1095  GACGGTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
             1701  GACTTTGTC           
BalI         3776  TGGCCA               TGG^CCA
             4318  TGGCCA              
BbrPI        3766  CACGTG               CAC^GTG
             4852  CACGTG              
BspHI         755  TCATGA               T^CATGA
             3226  TCATGA              
BssSI        2841  CTCGTG               CACGAG(-5/-1)
             3772  CTCGTG              
BstAPI       3104  GCACCTGTTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
             3854  GCAGGCCCTGC         
HincII        818  GTCAAC               GT[CT]^[GA]AC
             3702  GTTGAC              
HindII        818  GTCAAC               GT[CT]^[GA]AC
             3702  GTTGAC              
MluNI        3776  TGGCCA               TGG^CCA
             4318  TGGCCA              
MscI         3776  TGGCCA               TGG^CCA
             4318  TGGCCA              
NcoI          212  CCATGG               C^CATGG
             3119  CCATGG              
PflFI        1095  GACGGTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
             1701  GACTTTGTC           
PmaCI        3766  CACGTG               CAC^GTG
             4852  CACGTG              
PmlI         3766  CACGTG               CAC^GTG
             4852  CACGTG              
RcaI          755  TCATGA               T^CATGA
             3226  TCATGA              
SapI         3086  GAAGAGC              GCTCTTC(1/4)
             4128  GCTCTTC             
SexAI        3205  ACCAGGT              A^CC[AT]GGT
             3415  ACCTGGT             
Tth111I      1095  GACGGTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
             1701  GACTTTGTC           


#########################################
# 3 OR MORE CUT ENZYMES (  145 enzymes) #
#########################################

AccII       AciI        AcyI        AfaI        AluI        
AlwI        AlwNI       ApaI        AspHI       AvaI        
AvaII       AvrII       BamHI       BanI        BanII       
BbeI        BbsI        BbvI        BcnI        BfaI        
BglI        BlnI        BlpI        BmyI        BpmI        
Bpu1102I    BpuAI       BsaAI       BsaHI       BsaI        
BsaJI       BsaWI       BseRI       BsgI        BsiEI       
BsiHKAI     BsiYI       BslI        BsmAI       BsmBI       
BsmFI       BsoBI       Bsp1286I    BspMI       BsrDI       
BsrFI       BsrI        BssHII      BssKI       BstF5I      
BstNI       BstUI       BstXI       BstYI       Cac8I       
CelII       CfoI        Cfr10I      Cfr13I      DdeI        
DpnII       DraII       DraIII      DsaI        EaeI        
EagI        EarI        EclXI       Eco52I      EcoNI       
EcoO109I    EcoRII      EcoT14I     FauI        Fnu4HI      
FokI        HaeII       HaeIII      HapII       HgaI        
HhaI        Hin1I       HinP1I      HinfI       HpaII       
HphI        ItaI        KasI        Ksp632I     KspI        
MaeI        MaeII       MaeIII      MboI        MboII       
MflI        MnlI        MseI        MslI        MspA1I      
MspI        MvaI        MvnI        MwoI        NaeI        
NarI        NciI        NdeII       NgoMIV      NlaIII      
NlaIV       NspI        PflMI       PleI        PpuMI       
PstI        PvuII       RsaI        SacI        SacII       
Sau3AI      Sau96I      SbfI        ScrFI       SfaNI       
SfcI        SfoI        SmaI        SmlI        Sse8387I    
StuI        StyI        TaqI        TfiI        Tru9I       
TseI        Tsp45I      Tsp509I     TspRI       TthHB8I     
Van91I      XcmI        XhoII       XmaCI       XmaI