################################## # NO CUT ENZYMES ( 59 enzymes) # ################################## AatII AclI AflII AhdI Aor51HI AscI AseI AsnI Asp700I AspEI AviII BbvCI BfrI BglII BsaBI BsiWI Bsp1407I BspLU11I BsrGI Bst1107I BstBI BstZ17I CpoI DraI DrdI Eam1105I Eco47III FspI HindIII HpaI MamI MfeI MluI MunI NdeI NheI NotI NruI NspV PacI PaeR7I PmeI PshAI PshBI Psp1406I PvuI RsrII SalI ScaI SfuI SgrAI SnaBI SpeI SspBI SspI SwaI XbaI XhoI XmnI ################################### # ONE CUT ENZYMES ( 33 enzymes) # ################################### Acc65I 3209 GGTACC G^GTACC AccIII 1445 TCCGGA T^CCGGA AflIII 4851 ACACGT A^C[GA][CT]GT AgeI 4592 ACCGGT A^CCGGT Alw44I 3201 GTGCAC G^TGCAC AocI 220 CCTCAGG CC^T[ACGT]AGG ApaLI 3201 GTGCAC G^TGCAC Asp718I 3209 GGTACC G^GTACC BcgI 2791 GCAAGAAGTTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BciVI 639 GGATAC GTATCC(6/5) BclI 4776 TGATCA T^GATCA BseAI 1445 TCCGGA T^CCGGA BsmI 4292 GCATTC GAATGC(1/-1) BspDI 4011 ATCGAT AT^CGAT BspEI 1445 TCCGGA T^CCGGA BsrBI 4734 GAGCGG CCGCTC(-3/-3) BstEII 1499 GGTGACC G^GT[ACGT]ACC BstPI 1499 GGTGACC G^GT[ACGT]ACC Bsu36I 220 CCTCAGG CC^T[ACGT]AGG ClaI 4011 ATCGAT AT^CGAT Eco81I 220 CCTCAGG CC^T[ACGT]AGG EcoO65I 1499 GGTGACC G^GT[ACGT]ACC EcoRI 516 GAATTC G^AATTC EcoRV 2049 GATATC GAT^ATC EcoT22I 4872 ATGCAT ATGCA^T FbaI 4776 TGATCA T^GATCA FseI 2498 GGCCGGCC GGCCGG^CC KpnI 3209 GGTACC GGTAC^C MroI 1445 TCCGGA T^CCGGA NsiI 4872 ATGCAT ATGCA^T PinAI 4592 ACCGGT A^CCGGT SfiI 2468 GGCCGCGCTGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC SphI 3505 GCATGC GCATG^C ################################### # TWO CUT ENZYMES ( 21 enzymes) # ################################### AccI 1398 GTCTAC GT^[AC][GT]AC 4947 GTCTAC AcsI 516 GAATTC [GA]^AATT[CT] 4526 AAATTC ApoI 516 GAATTC [GA]^AATT[CT] 4526 AAATTC AspI 1095 GACGGTGTC GAC[ACGT]^[ACGT][ACGT]GTC 1701 GACTTTGTC BalI 3776 TGGCCA TGG^CCA 4318 TGGCCA BbrPI 3766 CACGTG CAC^GTG 4852 CACGTG BspHI 755 TCATGA T^CATGA 3226 TCATGA BssSI 2841 CTCGTG CACGAG(-5/-1) 3772 CTCGTG BstAPI 3104 GCACCTGTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC 3854 GCAGGCCCTGC HincII 818 GTCAAC GT[CT]^[GA]AC 3702 GTTGAC HindII 818 GTCAAC GT[CT]^[GA]AC 3702 GTTGAC MluNI 3776 TGGCCA TGG^CCA 4318 TGGCCA MscI 3776 TGGCCA TGG^CCA 4318 TGGCCA NcoI 212 CCATGG C^CATGG 3119 CCATGG PflFI 1095 GACGGTGTC GAC[ACGT]^[ACGT][ACGT]GTC 1701 GACTTTGTC PmaCI 3766 CACGTG CAC^GTG 4852 CACGTG PmlI 3766 CACGTG CAC^GTG 4852 CACGTG RcaI 755 TCATGA T^CATGA 3226 TCATGA SapI 3086 GAAGAGC GCTCTTC(1/4) 4128 GCTCTTC SexAI 3205 ACCAGGT A^CC[AT]GGT 3415 ACCTGGT Tth111I 1095 GACGGTGTC GAC[ACGT]^[ACGT][ACGT]GTC 1701 GACTTTGTC ######################################### # 3 OR MORE CUT ENZYMES ( 145 enzymes) # ######################################### AccII AciI AcyI AfaI AluI AlwI AlwNI ApaI AspHI AvaI AvaII AvrII BamHI BanI BanII BbeI BbsI BbvI BcnI BfaI BglI BlnI BlpI BmyI BpmI Bpu1102I BpuAI BsaAI BsaHI BsaI BsaJI BsaWI BseRI BsgI BsiEI BsiHKAI BsiYI BslI BsmAI BsmBI BsmFI BsoBI Bsp1286I BspMI BsrDI BsrFI BsrI BssHII BssKI BstF5I BstNI BstUI BstXI BstYI Cac8I CelII CfoI Cfr10I Cfr13I DdeI DpnII DraII DraIII DsaI EaeI EagI EarI EclXI Eco52I EcoNI EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeII HaeIII HapII HgaI HhaI Hin1I HinP1I HinfI HpaII HphI ItaI KasI Ksp632I KspI MaeI MaeII MaeIII MboI MboII MflI MnlI MseI MslI MspA1I MspI MvaI MvnI MwoI NaeI NarI NciI NdeII NgoMIV NlaIII NlaIV NspI PflMI PleI PpuMI PstI PvuII RsaI SacI SacII Sau3AI Sau96I SbfI ScrFI SfaNI SfcI SfoI SmaI SmlI Sse8387I StuI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I Van91I XcmI XhoII XmaCI XmaI