##################################
# NO CUT ENZYMES ( 84 enzymes) #
##################################
AatII AccII AccIII AcyI AgeI
AhdI Alw44I Aor51HI ApaLI AscI
Asp700I AspEI AspI AviII BbeI
BcgI BclI BglI BglII BlpI
Bpu1102I BsaHI BsaI BsaWI BseAI
BsiWI BsmBI Bsp1407I BspDI BspEI
BsrBI BsrFI BsrGI BssHII Bst1107I
BstAPI BstBI BstUI BstZ17I CelII
Cfr10I ClaI CpoI DraIII DrdI
Eam1105I Eco47III EcoRV FbaI FseI
FspI Hin1I HpaI KasI KspI
MluI MroI MvnI NaeI NarI
NgoMIV NotI NruI NspV PflFI
PinAI PmeI PvuI RsrII SacII
SalI SapI SbfI SexAI SfiI
SfoI SfuI SgrAI SphI Sse8387I
SspBI Tth111I XbaI XmnI
###################################
# ONE CUT ENZYMES ( 47 enzymes) #
###################################
AccI 3851 GTCTAC GT^[AC][GT]AC
AclI 1216 AACGTT AA^CGTT
AocI 4347 CCTTAGG CC^T[ACGT]AGG
ApaI 3629 GGGCCC GGGCC^C
AspHI 300 GAGCTC G[AT]GC[AT]^C
BalI 3133 TGGCCA TGG^CCA
BbrPI 2028 CACGTG CAC^GTG
BsaBI 2840 GATTTAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsiHKAI 300 GAGCTC G[AT]GC[AT]^C
BspHI 2813 TCATGA T^CATGA
BssSI 825 CACGAG CACGAG(-5/-1)
BstEII 972 GGTTACC G^GT[ACGT]ACC
BstPI 972 GGTTACC G^GT[ACGT]ACC
BstXI 4550 CCATTATTATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 4347 CCTTAGG CC^T[ACGT]AGG
CfoI 1134 GCGC GCG^C
EagI 4911 CGGCCG C^GGCCG
EclXI 4911 CGGCCG C^GGCCG
Eco52I 4911 CGGCCG C^GGCCG
Eco81I 4347 CCTTAGG CC^T[ACGT]AGG
EcoO65I 972 GGTTACC G^GT[ACGT]ACC
HaeII 1133 AGCGCC [GA]GCGC^[CT]
HgaI 5113 GACGC GACGC(5/10)
HhaI 1134 GCGC GCG^C
HinP1I 1134 GCGC G^CGC
HincII 2068 GTCAAC GT[CT]^[GA]AC
HindII 2068 GTCAAC GT[CT]^[GA]AC
MamI 2840 GATTTAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluNI 3133 TGGCCA TGG^CCA
MscI 3133 TGGCCA TGG^CCA
NheI 2774 GCTAGC G^CTAGC
PacI 3523 TTAATTAA TTAAT^TAA
PaeR7I 513 CTCGAG C^TCGAG
PflMI 226 CCAGCCCATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI 2028 CACGTG CAC^GTG
PmlI 2028 CACGTG CAC^GTG
PpuMI 3420 AGGTCCT [GA]G^G[AT]CC[CT]
PshAI 2987 GACTCCTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
Psp1406I 1216 AACGTT AA^CGTT
PstI 645 CTGCAG CTGCA^G
RcaI 2813 TCATGA T^CATGA
SacI 300 GAGCTC GAGCT^C
SnaBI 2443 TACGTA TAC^GTA
StuI 5271 AGGCCT AGG^CCT
SwaI 2841 ATTTAAAT ATTT^AAAT
Van91I 226 CCAGCCCATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoI 513 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 33 enzymes) #
###################################
Acc65I 559 GGTACC G^GTACC
2545 GGTACC
AflII 2271 CTTAAG C^TTAAG
3883 CTTAAG
AflIII 2348 ACATGT A^C[GA][CT]GT
2377 ACATGT
AseI 2460 ATTAAT AT^TAAT
4767 ATTAAT
AsnI 2460 ATTAAT AT^TAAT
4767 ATTAAT
Asp718I 559 GGTACC G^GTACC
2545 GGTACC
AvrII 1916 CCTAGG C^CTAGG
3985 CCTAGG
BbvCI 1622 CCTCAGC CCTCAGC(-5/-2)
4878 CCTCAGC
BciVI 4038 GGATAC GTATCC(6/5)
4274 GGATAC
BfrI 2271 CTTAAG C^TTAAG
3883 CTTAAG
BlnI 1916 CCTAGG C^CTAGG
3985 CCTAGG
BsaAI 2028 CACGTG [CT]AC^GT[GA]
2443 TACGTA
BsiEI 4911 CGGCCG CG[GA][CT]^CG
4956 CGACCG
BsmI 528 GAATGC GAATGC(1/-1)
1721 GAATGC
BspLU11I 2348 ACATGT A^CATGT
2377 ACATGT
DraI 2842 TTTAAA TTT^AAA
4785 TTTAAA
EaeI 3133 TGGCCA [CT]^GGCC[GA]
4911 CGGCCG
EcoNI 4211 CCTAGCTCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
4820 CCTTTTATAGG
EcoRI 1296 GAATTC G^AATTC
3398 GAATTC
EcoT22I 1445 ATGCAT ATGCA^T
2053 ATGCAT
FauI 108 GCGGG CCCGC(4/6)
1331 GCGGG
KpnI 559 GGTACC GGTAC^C
2545 GGTACC
MfeI 1272 CAATTG C^AATTG
2834 CAATTG
MslI 2814 CATGAGAATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
4551 CATTATTATG
MunI 1272 CAATTG C^AATTG
2834 CAATTG
NsiI 1445 ATGCAT ATGCA^T
2053 ATGCAT
PshBI 2460 ATTAAT AT^TAAT
4767 ATTAAT
ScaI 140 AGTACT AGT^ACT
1813 AGTACT
SmaI 295 CCCGGG CCC^GGG
5029 CCCGGG
SpeI 959 ACTAGT A^CTAGT
3782 ACTAGT
XcmI 1673 CCAGATCCTGCTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
5219 CCAAAGGCCAGCTGG
XmaCI 295 CCCGGG C^CCGGG
5029 CCCGGG
XmaI 295 CCCGGG C^CCGGG
5029 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 94 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
AlwNI ApoI AvaI AvaII BamHI
BanI BanII BbsI BbvI BcnI
BfaI BmyI BpmI BpuAI BsaJI
BseRI BsgI BsiYI BslI BsmAI
BsmFI BsoBI Bsp1286I BspMI BsrDI
BsrI BssKI BstF5I BstNI BstYI
Cac8I Cfr13I DdeI DpnII DraII
DsaI EarI EcoO109I EcoRII EcoT14I
Fnu4HI FokI HaeIII HapII HindIII
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeII MaeIII MboI MboII
MflI MnlI MseI MspA1I MspI
MvaI MwoI NciI NcoI NdeI
NdeII NlaIII NlaIV NspI PleI
PvuII RsaI Sau3AI Sau96I ScrFI
SfaNI SfcI SmlI SspI StyI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I XhoII